Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic precursor

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Q9M591 (CRD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic
Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC=1.14.13.81

Alternative name(s):
Copper response defect 1 protein
Dicarboxylate diiron protein
Short name=AtZIP

Gene names

Name:	CRD1
Synonyms:	AT103, CHL27, ZIP
Ordered Locus Names:	At3g56940
ORF Names:	F24I3.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.7
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13₁-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O. Ref.7 13₁-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13₁-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O. Ref.7 13₁-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O. Ref.7
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast inner membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein Ref.7.
Sequence similarities	Belongs to the AcsF family.
Sequence caution	The sequence AAB18942.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Membrane Plastid Plastid inner membrane Thylakoid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast inner membrane Inferred from direct assay Ref.7. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay Ref.7. Source: TAIR
Molecular function	DNA binding Traceable author statement. Source: TAIR magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from mutant phenotype Ref.7. Source: TAIR transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9M591-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 36

Chloroplast Potential

Chain

37 – 409

373

Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic

PRO_0000000598

Experimental info

Sequence conflict

F → I in AAB18942. Ref.1

Sequence conflict

157

L → S in AAB18942. Ref.1

Sequence conflict

216 – 219

TYLS → RAAR in AAB51703. Ref.6

Sequence conflict

299

C → W in AAF63476. Ref.2

Sequence conflict

310 – 312

LNT → FKH in AAB51703. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: B8808079C07B8C68
Show »

FASTA

409

47,631

        10         20         30         40         50         60 
MAAEMALVKP ISKFSSPKLS NPSKFLSGRR FSTVIRMSAS SSPPPPTTAT SKSKKGTKKE 

        70         80         90        100        110        120 
IQESLLTPRF YTTDFEEMEQ LFNTEINKNL NEAEFEALLQ EFKTDYNQTH FVRNKEFKEA 

       130        140        150        160        170        180 
ADKLQGPLRQ IFVEFLERSC TAEFSGFLLY KELGRRLKKT NPVVAEIFSL MSRDEARHAG 

       190        200        210        220        230        240 
FLNKGLSDFN LALDLGFLTK ARKYTFFKPK FIFYATYLSE KIGYWRYITI YRHLKENPEF 

       250        260        270        280        290        300 
QCYPIFKYFE NWCQDENRHG DFFSALMKAQ PQFLNDWQAK LWSRFFCLSV YVTMYLNDCQ 

       310        320        330        340        350        360 
RTNFYEGIGL NTKEFDMHVI IETNRTTARI FPAVLDVENP EFKRKLDRMV VSYEKLLAIG 

       370        380        390        400 
ETDDASFIKT LKRIPLVTSL ASEILAAYLM PPVESGSVDF AEFEPNLVY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PNZIP is a novel mesophyll-specific cDNA that is regulated by phytochrome and the circadian rhythm and encodes a protein with a leucine zipper motif." Zheng C.C., Porat R., Lu P., O'Neill S.D. Plant Physiol. 116:27-35(1998) [PubMed: 9449833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii." Moseley J.L., Quinn J., Eriksson M., Merchant S. EMBO J. 19:2139-2151(2000) [PubMed: 10811605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Cloning and characteristics of AT103 gene promoter." Liu N., Zheng C. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-76.
[6]	"Isolation of molecular markers for salt stress responses in Arabidopsis thaliana." Pih K.T., Jang H.J., Kang S.G., Piao H.L., Hwang I. Mol. Cells 7:567-571(1997) [PubMed: 9339905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 215-313. Strain: cv. Columbia.
[7]	"Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide." Tottey S., Block M.A., Allen M., Westergren T., Albrieux C., Scheller H.V., Merchant S., Jensen P.E. Proc. Natl. Acad. Sci. U.S.A. 100:16119-16124(2003) [PubMed: 14673103] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38232 mRNA. Translation: AAB18942.1. Different initiation. AF236101 mRNA. Translation: AAF63476.1. AL138655 Genomic DNA. Translation: CAB72164.1. CP002686 Genomic DNA. Translation: AEE79589.1. AY170319 Genomic DNA. Translation: AAO11785.1. U75599 mRNA. Translation: AAB51703.1.
IPI	IPI00517126.
PIR	T47754.
RefSeq	NP_191253.1. NM_115553.4.
UniGene	At.48778. At.70999.
3D structure databases
ProteinModelPortal	Q9M591.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9M591.
Proteomic databases
PRIDE	Q9M591.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G56940.1; AT3G56940.1; AT3G56940.
GeneID	824861.
GenomeReviews	Gene locus AT3G56940 in contig BA000014_GR.
KEGG	ath:AT3G56940.
NMPDR	fig\|3702.1.peg.17023.
Organism-specific databases
TAIR	At3g56940.
Phylogenomic databases
eggNOG	NOG10633.
GeneTree	EPGT00050000018409.
HOGENOM	HBG284843.
InParanoid	Q9M591.
OMA	HGDFFDA.
PhylomeDB	Q9M591.
ProtClustDB	PLN02508.
Enzyme and pathway databases
BioCyc	ARA:AT3G56940-MONOMER. MetaCyc:AT3G56940-MONOMER.
Gene expression databases
Genevestigator	Q9M591.
GermOnline	AT3G56940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008434. AcsF. IPR012347. Ferritin-rel. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KO	K04035.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_ARATH

Accession

Primary (citable) accession number: Q9M591
Secondary accession number(s): O04051

Q9M1K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	December 14, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents