Reviewed, UniProtKB/Swiss-Prot Q9M591 (CRD1_ARATH)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81
Alternative name(s):
    Copper response defect 1 protein
    Dicarboxylate diiron protein
      Short name=AtZIP

Gene names

Name:	CRD1
Synonyms:	AT103, CHL27, ZIP
Ordered Locus Names:	At3g56940
ORF Names:	F24I3.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.6
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. Ref.6 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. Ref.6 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. Ref.6
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast inner membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein. Ref.6
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Membrane Plastid Plastid inner membrane Thylakoid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast inner membrane Ref.6 Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Ref.6 Inferred from direct assay. Source: TAIR
Molecular function	DNA binding Traceable author statement. Source: TAIR iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Ref.6 Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Chloroplast Potential
	Chain	37 – 409	373	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic		PRO_0000000598
Experimental info
	Sequence conflict	14	1	F → I Ref.1
	Sequence conflict	157	1	L → S in AAB18942. Ref.1
	Sequence conflict	216 – 219	4	TYLS → RAAR Ref.5
	Sequence conflict	299	1	C → W in AAF63476. Ref.2
	Sequence conflict	310 – 312	3	LNT → FKH Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9M591-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: B8808079C07B8C68	FASTA	409	47,631
	10 20 30 40 50 60 MAAEMALVKP ISKFSSPKLS NPSKFLSGRR FSTVIRMSAS SSPPPPTTAT SKSKKGTKKE 70 80 90 100 110 120 IQESLLTPRF YTTDFEEMEQ LFNTEINKNL NEAEFEALLQ EFKTDYNQTH FVRNKEFKEA 130 140 150 160 170 180 ADKLQGPLRQ IFVEFLERSC TAEFSGFLLY KELGRRLKKT NPVVAEIFSL MSRDEARHAG 190 200 210 220 230 240 FLNKGLSDFN LALDLGFLTK ARKYTFFKPK FIFYATYLSE KIGYWRYITI YRHLKENPEF 250 260 270 280 290 300 QCYPIFKYFE NWCQDENRHG DFFSALMKAQ PQFLNDWQAK LWSRFFCLSV YVTMYLNDCQ 310 320 330 340 350 360 RTNFYEGIGL NTKEFDMHVI IETNRTTARI FPAVLDVENP EFKRKLDRMV VSYEKLLAIG 370 380 390 400 ETDDASFIKT LKRIPLVTSL ASEILAAYLM PPVESGSVDF AEFEPNLVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PNZIP is a novel mesophyll-specific cDNA that is regulated by phytochrome and the circadian rhythm and encodes a protein with a leucine zipper motif." Zheng C.C., Porat R., Lu P., O'Neill S.D. Plant Physiol. 116:27-35(1998) [PubMed: 9449833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii." Moseley J.L., Quinn J., Eriksson M., Merchant S. EMBO J. 19:2139-2151(2000) [PubMed: 10811605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Cloning and characteristics of AT103 gene promoter." Liu N., Zheng C. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-76.
[5]	"Isolation of molecular markers for salt stress responses in Arabidopsis thaliana." Pih K.T., Jang H.J., Kang S.G., Piao H.L., Hwang I. Mol. Cells 7:567-571(1997) [PubMed: 9339905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 215-313. Strain: cv. Columbia.
[6]	"Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide." Tottey S., Block M.A., Allen M., Westergren T., Albrieux C., Scheller H.V., Merchant S., Jensen P.E. Proc. Natl. Acad. Sci. U.S.A. 100:16119-16124(2003) [PubMed: 14673103] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38232 mRNA. Translation: AAB18942.1. Different initiation. AF236101 mRNA. Translation: AAF63476.1. AL138655 Genomic DNA. Translation: CAB72164.1. AY170319 Genomic DNA. Translation: AAO11785.1. U75599 mRNA. Translation: AAB51703.1.
IPI	IPI00517126.
PIR	T47754.
RefSeq	NP_191253.1.
UniGene	At.48778
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9M591.
Genome annotation databases
GeneID	824861.
GenomeReviews	Gene locus AT3G56940 in contig BA000014_GR.
KEGG	ath:AT3G56940.
NMPDR	fig|3702.1.peg.17023.
Organism-specific databases
TAIR	At3g56940.
Phylogenomic databases
OMA	Q9M591. TAEFSGC.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G56940-MON.
BRENDA	1.14.13.81. 302.
Gene expression databases
GermOnline	AT3G56940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_ARATH

Accession

Primary (citable) accession number: Q9M591
Secondary accession number(s): O04051 , Q38892, Q8GUS3, Q9M1K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)