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Protein

DNA (cytosine-5)-methyltransferase DRM2

Gene

DRM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to fungus Source: TAIR
  2. DNA methylation Source: TAIR
  3. gene silencing Source: UniProtKB
  4. histone H3-K9 methylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G14620-MONOMER.

Protein family/group databases

REBASEi4601. M.AthDRM2.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase DRM2 (EC:2.1.1.37)
Alternative name(s):
Protein DOMAINS REARRANGED METHYLASE 2
Gene namesi
Name:DRM2
Ordered Locus Names:At5g14620/At5g14630
ORF Names:T15N1.110/T15N1.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G14620.

Subcellular locationi

Nucleusnucleoplasm 1 Publication
Note: Peri-nucleolar.

GO - Cellular componenti

  1. nucleoplasm Source: UniProtKB-SubCell
  2. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626DNA (cytosine-5)-methyltransferase DRM2PRO_0000381942Add
BLAST

Proteomic databases

PaxDbiQ9M548.
PRIDEiQ9M548.

Expressioni

Tissue specificityi

Expressed in roots, inflorescences and at lower levels in leaves.1 Publication

Gene expression databases

GenevestigatoriQ9M548.

Interactioni

Subunit structurei

Interacts with RDM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO4Q9ZVD52EBI-6923904,EBI-2352199

Protein-protein interaction databases

BioGridi16592. 2 interactions.
DIPiDIP-59279N.
IntActiQ9M548. 8 interactions.
STRINGi3702.AT5G14620.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M548.
SMRiQ9M548. Positions 276-623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 15042UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini190 – 23243UBA 2PROSITE-ProRule annotationAdd
BLAST
Domaini295 – 626332SAM-dependent MTase DRM-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DRM-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase DRM-type domain.PROSITE-ProRule annotation
Contains 2 UBA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG70699.
HOGENOMiHOG000030355.
InParanoidiQ9M548.
OMAiHSSLFYH.
PhylomeDBiQ9M548.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR030380. SAM_MeTfrase_DRM.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF53335. SSF53335. 2 hits.
PROSITEiPS51680. SAM_MT_DRM. 1 hit.
PS50030. UBA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVIWNNDDDD FLEIDNFQSS PRSSPIHAMQ CRVENLAGVA VTTSSLSSPT
60 70 80 90 100
ETTDLVQMGF SDEVFATLFD MGFPVEMISR AIKETGPNVE TSVIIDTISK
110 120 130 140 150
YSSDCEAGSS KSKAIDHFLA MGFDEEKVVK AIQEHGEDNM EAIANALLSC
160 170 180 190 200
PEAKKLPAAV EEEDGIDWSS SDDDTNYTDM LNSDDEKDPN SNENGSKIRS
210 220 230 240 250
LVKMGFSELE ASLAVERCGE NVDIAELTDF LCAAQMAREF SEFYTEHEEQ
260 270 280 290 300
KPRHNIKKRR FESKGEPRSS VDDEPIRLPN PMIGFGVPNE PGLITHRSLP
310 320 330 340 350
ELARGPPFFY YENVALTPKG VWETISRHLF EIPPEFVDSK YFCVAARKRG
360 370 380 390 400
YIHNLPINNR FQIQPPPKYT IHDAFPLSKR WWPEWDKRTK LNCILTCTGS
410 420 430 440 450
AQLTNRIRVA LEPYNEEPEP PKHVQRYVID QCKKWNLVWV GKNKAAPLEP
460 470 480 490 500
DEMESILGFP KNHTRGGGMS RTERFKSLGN SFQVDTVAYH LSVLKPIFPH
510 520 530 540 550
GINVLSLFTG IGGGEVALHR LQIKMKLVVS VEISKVNRNI LKDFWEQTNQ
560 570 580 590 600
TGELIEFSDI QHLTNDTIEG LMEKYGGFDL VIGGSPCNNL AGGNRVSRVG
610 620
LEGDQSSLFF EYCRILEVVR ARMRGS
Length:626
Mass (Da):70,431
Last modified:October 1, 2000 - v1
Checksum:i10A38FC068636DB7
GO

Sequence cautioni

The sequence CAB87629.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.Curated
The sequence CAB87630.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240695 mRNA. Translation: AAF66129.1.
AL163792 Genomic DNA. Translation: CAB87629.1. Sequence problems.
AL163792 Genomic DNA. Translation: CAB87630.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92056.1.
AK176138 mRNA. Translation: BAD43901.1.
AK220953 mRNA. Translation: BAD94486.1.
AK229197 mRNA. Translation: BAF01067.1.
PIRiT48635.
T48636.
RefSeqiNP_196966.2. NM_121466.2.
UniGeneiAt.5084.

Genome annotation databases

EnsemblPlantsiAT5G14620.1; AT5G14620.1; AT5G14620.
GeneIDi831315.
KEGGiath:AT5G14620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240695 mRNA. Translation: AAF66129.1.
AL163792 Genomic DNA. Translation: CAB87629.1. Sequence problems.
AL163792 Genomic DNA. Translation: CAB87630.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92056.1.
AK176138 mRNA. Translation: BAD43901.1.
AK220953 mRNA. Translation: BAD94486.1.
AK229197 mRNA. Translation: BAF01067.1.
PIRiT48635.
T48636.
RefSeqiNP_196966.2. NM_121466.2.
UniGeneiAt.5084.

3D structure databases

ProteinModelPortaliQ9M548.
SMRiQ9M548. Positions 276-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16592. 2 interactions.
DIPiDIP-59279N.
IntActiQ9M548. 8 interactions.
STRINGi3702.AT5G14620.1-P.

Protein family/group databases

REBASEi4601. M.AthDRM2.

Proteomic databases

PaxDbiQ9M548.
PRIDEiQ9M548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G14620.1; AT5G14620.1; AT5G14620.
GeneIDi831315.
KEGGiath:AT5G14620.

Organism-specific databases

TAIRiAT5G14620.

Phylogenomic databases

eggNOGiNOG70699.
HOGENOMiHOG000030355.
InParanoidiQ9M548.
OMAiHSSLFYH.
PhylomeDBiQ9M548.

Enzyme and pathway databases

BioCyciARA:AT5G14620-MONOMER.

Gene expression databases

GenevestigatoriQ9M548.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR030380. SAM_MeTfrase_DRM.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF53335. SSF53335. 2 hits.
PROSITEiPS51680. SAM_MT_DRM. 1 hit.
PS50030. UBA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved plant genes with similarity to mammalian de novo DNA methyltransferases."
    Cao X., Springer N.M., Muszynski M.G., Phillips R.L., Kaeppler S., Jacobsen S.E.
    Proc. Natl. Acad. Sci. U.S.A. 97:4979-4984(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes."
    Cao X., Jacobsen S.E.
    Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing."
    Cao X., Jacobsen S.E.
    Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
    Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
    Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: INTERACTION WITH RDM1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDRM2_ARATH
AccessioniPrimary (citable) accession number: Q9M548
Secondary accession number(s): Q9LYJ7, Q9LYJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.