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Q9M548

- DRM2_ARATH

UniProt

Q9M548 - DRM2_ARATH

Protein

DNA (cytosine-5)-methyltransferase DRM2

Gene

DRM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.3 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. defense response to fungus Source: TAIR
    2. DNA methylation Source: TAIR
    3. gene silencing Source: UniProtKB
    4. histone H3-K9 methylation Source: TAIR

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT5G14620-MONOMER.

    Protein family/group databases

    REBASEi4601. M.AthDRM2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase DRM2 (EC:2.1.1.37)
    Alternative name(s):
    Protein DOMAINS REARRANGED METHYLASE 2
    Gene namesi
    Name:DRM2
    Ordered Locus Names:At5g14620/At5g14630
    ORF Names:T15N1.110/T15N1.120
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G14620.

    Subcellular locationi

    Nucleusnucleoplasm 1 Publication
    Note: Peri-nucleolar.

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB-SubCell
    2. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 626626DNA (cytosine-5)-methyltransferase DRM2PRO_0000381942Add
    BLAST

    Proteomic databases

    PaxDbiQ9M548.
    PRIDEiQ9M548.

    Expressioni

    Tissue specificityi

    Expressed in roots, inflorescences and at lower levels in leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ9M548.

    Interactioni

    Subunit structurei

    Interacts with RDM1.1 Publication

    Protein-protein interaction databases

    BioGridi16592. 2 interactions.
    DIPiDIP-59279N.
    IntActiQ9M548. 1 interaction.
    STRINGi3702.AT5G14620.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M548.
    SMRiQ9M548. Positions 502-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini109 – 15042UBA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini190 – 23243UBA 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini295 – 626332SAM-dependent MTase DRM-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DRM-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase DRM-type domain.PROSITE-ProRule annotation
    Contains 2 UBA domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG70699.
    HOGENOMiHOG000030355.
    InParanoidiQ9M548.
    OMAiRTKLNCI.
    PhylomeDBiQ9M548.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR001525. C5_MeTfrase.
    IPR025822. C5_MeTrfase_DMR.
    IPR029063. SAM-dependent_MTases-like.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view]
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    SMARTiSM00165. UBA. 3 hits.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 3 hits.
    SSF53335. SSF53335. 2 hits.
    PROSITEiPS51680. SAM_MT_DRM. 1 hit.
    PS50030. UBA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9M548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVIWNNDDDD FLEIDNFQSS PRSSPIHAMQ CRVENLAGVA VTTSSLSSPT    50
    ETTDLVQMGF SDEVFATLFD MGFPVEMISR AIKETGPNVE TSVIIDTISK 100
    YSSDCEAGSS KSKAIDHFLA MGFDEEKVVK AIQEHGEDNM EAIANALLSC 150
    PEAKKLPAAV EEEDGIDWSS SDDDTNYTDM LNSDDEKDPN SNENGSKIRS 200
    LVKMGFSELE ASLAVERCGE NVDIAELTDF LCAAQMAREF SEFYTEHEEQ 250
    KPRHNIKKRR FESKGEPRSS VDDEPIRLPN PMIGFGVPNE PGLITHRSLP 300
    ELARGPPFFY YENVALTPKG VWETISRHLF EIPPEFVDSK YFCVAARKRG 350
    YIHNLPINNR FQIQPPPKYT IHDAFPLSKR WWPEWDKRTK LNCILTCTGS 400
    AQLTNRIRVA LEPYNEEPEP PKHVQRYVID QCKKWNLVWV GKNKAAPLEP 450
    DEMESILGFP KNHTRGGGMS RTERFKSLGN SFQVDTVAYH LSVLKPIFPH 500
    GINVLSLFTG IGGGEVALHR LQIKMKLVVS VEISKVNRNI LKDFWEQTNQ 550
    TGELIEFSDI QHLTNDTIEG LMEKYGGFDL VIGGSPCNNL AGGNRVSRVG 600
    LEGDQSSLFF EYCRILEVVR ARMRGS 626
    Length:626
    Mass (Da):70,431
    Last modified:October 1, 2000 - v1
    Checksum:i10A38FC068636DB7
    GO

    Sequence cautioni

    The sequence CAB87629.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.
    The sequence CAB87630.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240695 mRNA. Translation: AAF66129.1.
    AL163792 Genomic DNA. Translation: CAB87629.1. Sequence problems.
    AL163792 Genomic DNA. Translation: CAB87630.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92056.1.
    AK176138 mRNA. Translation: BAD43901.1.
    AK220953 mRNA. Translation: BAD94486.1.
    AK229197 mRNA. Translation: BAF01067.1.
    PIRiT48635.
    T48636.
    RefSeqiNP_196966.2. NM_121466.2.
    UniGeneiAt.5084.

    Genome annotation databases

    EnsemblPlantsiAT5G14620.1; AT5G14620.1; AT5G14620.
    GeneIDi831315.
    KEGGiath:AT5G14620.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240695 mRNA. Translation: AAF66129.1 .
    AL163792 Genomic DNA. Translation: CAB87629.1 . Sequence problems.
    AL163792 Genomic DNA. Translation: CAB87630.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92056.1 .
    AK176138 mRNA. Translation: BAD43901.1 .
    AK220953 mRNA. Translation: BAD94486.1 .
    AK229197 mRNA. Translation: BAF01067.1 .
    PIRi T48635.
    T48636.
    RefSeqi NP_196966.2. NM_121466.2.
    UniGenei At.5084.

    3D structure databases

    ProteinModelPortali Q9M548.
    SMRi Q9M548. Positions 502-622.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16592. 2 interactions.
    DIPi DIP-59279N.
    IntActi Q9M548. 1 interaction.
    STRINGi 3702.AT5G14620.1-P.

    Protein family/group databases

    REBASEi 4601. M.AthDRM2.

    Proteomic databases

    PaxDbi Q9M548.
    PRIDEi Q9M548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G14620.1 ; AT5G14620.1 ; AT5G14620 .
    GeneIDi 831315.
    KEGGi ath:AT5G14620.

    Organism-specific databases

    TAIRi AT5G14620.

    Phylogenomic databases

    eggNOGi NOG70699.
    HOGENOMi HOG000030355.
    InParanoidi Q9M548.
    OMAi RTKLNCI.
    PhylomeDBi Q9M548.

    Enzyme and pathway databases

    BioCyci ARA:AT5G14620-MONOMER.

    Gene expression databases

    Genevestigatori Q9M548.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR001525. C5_MeTfrase.
    IPR025822. C5_MeTrfase_DMR.
    IPR029063. SAM-dependent_MTases-like.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view ]
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    SMARTi SM00165. UBA. 3 hits.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 3 hits.
    SSF53335. SSF53335. 2 hits.
    PROSITEi PS51680. SAM_MT_DRM. 1 hit.
    PS50030. UBA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conserved plant genes with similarity to mammalian de novo DNA methyltransferases."
      Cao X., Springer N.M., Muszynski M.G., Phillips R.L., Kaeppler S., Jacobsen S.E.
      Proc. Natl. Acad. Sci. U.S.A. 97:4979-4984(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes."
      Cao X., Jacobsen S.E.
      Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing."
      Cao X., Jacobsen S.E.
      Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
      Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
      Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: INTERACTION WITH RDM1, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDRM2_ARATH
    AccessioniPrimary (citable) accession number: Q9M548
    Secondary accession number(s): Q9LYJ7, Q9LYJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3