Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9M547 (FLS_EUSER) Reviewed, UniProtKB/Swiss-Prot

Last modified November 28, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flavonol synthase/flavanone 3-hydroxylase
Short name=FLS
EC=1.14.11.23
EC=1.14.11.9
Gene names
Name:FLS
OrganismEustoma exaltatum subsp. russellianum (Bluebells) (Eustoma grandiflorum)
Taxonomic identifier52518 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Gentianaceae › Chironieae › Eustoma ›

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin By similarity.

Catalytic activity

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.

A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 ascorbate molecule per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Flavonol synthase/flavanone 3-hydroxylase
PRO_0000067293

Regions

Domain196 – 295100Fe2OG dioxygenase

Sites

Metal binding2201Iron By similarity
Metal binding2221Iron By similarity
Metal binding2761Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9M547 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4097830ABE2534E2

FASTA33438,081
        10         20         30         40         50         60 
MEVQRVQEIA SLSKVIDTIP AEYIRSENEQ PVISTVHGVV LEVPVIDLSD SDEKKIVGLV 

        70         80         90        100        110        120 
SEASKEWGIF QVVNHGIPNE VIRKLQEVGK HFFELPQEEK ELIAKPEGSQ SIEGYGTRLQ 

       130        140        150        160        170        180 
KEVDGKKGWV DHLFHKIWPP SAINYQFWPK NPPAYREANE EYAKRLQLVV DNLFKYLSLG 

       190        200        210        220        230        240 
LDLEPNSFKD GAGGDDLVYL MKINYYPPCP RPDLALGVAH TDMSAITVLV PNEVPGLQVY 

       250        260        270        280        290        300 
KDGHWYDCKY IPNALIVHIG DQVEIMSNGK YKSVYHRTTV NKEKTRMSWP VFLEPPPDHE 

       310        320        330 
VGPIPKLVNE ENPAKFKTKK YKDYAYCKLN KLPQ

« Hide

References

[1]"cDNA cloning of flavonol synthase and antisense suppression of expression in Lisianthus grandiflorum grise."
Nielsen K.M.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF240764 mRNA. Translation: AAF64168.1.

3D structure databases

ProteinModelPortalQ9M547.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

InterProIPR026992. DIOX_N.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFLS_EUSER
AccessionPrimary (citable) accession number: Q9M547
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: October 1, 2000
Last modified: November 28, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents