Reviewed, UniProtKB/Swiss-Prot Q9M547 (FLS_EUSGR)

Names and origin

Protein names

Recommended name:
    Flavonol synthase/flavanone 3-hydroxylase
      Short name=FLS
    EC=1.14.11.23
    EC=1.14.11.9

Gene names

Name:

FLS

Organism

Eustoma grandiflorum (Bluebells) (Lisianthus russellianus)

Taxonomic identifier

52518 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Gentianaceae › Chironieae › Eustoma

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin By similarity.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O. A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Flavonol synthase/flavanone 3-hydroxylase		PRO_0000067293
Sites
	Metal binding	220	1	Iron By similarity
	Metal binding	222	1	Iron By similarity
	Metal binding	276	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9M547-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4097830ABE2534E2	FASTA	334	38,081
	10 20 30 40 50 60 MEVQRVQEIA SLSKVIDTIP AEYIRSENEQ PVISTVHGVV LEVPVIDLSD SDEKKIVGLV 70 80 90 100 110 120 SEASKEWGIF QVVNHGIPNE VIRKLQEVGK HFFELPQEEK ELIAKPEGSQ SIEGYGTRLQ 130 140 150 160 170 180 KEVDGKKGWV DHLFHKIWPP SAINYQFWPK NPPAYREANE EYAKRLQLVV DNLFKYLSLG 190 200 210 220 230 240 LDLEPNSFKD GAGGDDLVYL MKINYYPPCP RPDLALGVAH TDMSAITVLV PNEVPGLQVY 250 260 270 280 290 300 KDGHWYDCKY IPNALIVHIG DQVEIMSNGK YKSVYHRTTV NKEKTRMSWP VFLEPPPDHE 310 320 330 VGPIPKLVNE ENPAKFKTKK YKDYAYCKLN KLPQ

References

[1]	"cDNA cloning of flavonol synthase and antisense suppression of expression in Lisianthus grandiflorum grise." Nielsen K.M. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF240764 mRNA. Translation: AAF64168.1.
3D structure databases
HSSP	HSSP built from PDB template 1GP6 based on UniProtKB Q96323.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.23. 276991. 1.14.11.9. 276991.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLS_EUSGR

Accession

Primary (citable) accession number: Q9M547

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)