Q9M4S8 (TPIC_FRAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase, chloroplastic
Short name=TIM
Short name=Triose-phosphate isomerase
EC=5.3.1.1

Gene names

Name:

TPI

Organism

Fragaria ananassa (Strawberry)

Taxonomic identifier

3747 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Rosoideae › Rosodae › Potentilleae › Fragaria

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Miscellaneous	In plants, there are two types of TPIS, cytosolic and plastid.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Chloroplast By similarity
	Chain	60 – 314	255	Triosephosphate isomerase, chloroplastic		PRO_0000035650
Sites
	Active site	154	1	Electrophile By similarity
	Active site	224	1	Proton acceptor By similarity
	Binding site	70	1	Substrate By similarity
	Binding site	72	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9M4S8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A7D8BBD98CE55853	FASTA	314	33,526
	10 20 30 40 50 60 MAVASTSLAS QLSGPKSLSQ PYSGLRRSCP KLDQSHSSLF QHLSLSSSSR KASRAVVAMA 70 80 90 100 110 120 GTGKFFVGGN WKCNGTKDLI SKLVSDLNSA KLEPDVDVVV APPFLYLDQV KSSLTDRIEI 130 140 150 160 170 180 SGQNSWVAKG GAFTGEISVE QLKDIGRKWV ILGHSERRHV IGEDDQFIGK KAAYALNEGL 190 200 210 220 230 240 GVIACIGEKL EEREAGKTFD VCYEQLKAFA DAVPSWENIV VAYEPVWAIG TGKVASPQQA 250 260 270 280 290 300 QEVHVAVREW LKKNVSAEVA SKTRIIYGGS VNGGNSAELA KEEDIDGFLV GGASLKGPEF 310 ATIVNAVTSK KVAA

References

[1]	"Cloning of a strawberry gene encoding a chloroplast triosephosphate isomerase." Kim I.-J., Chung W.-I. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Duch..
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF257322 mRNA. Translation: AAF66071.1.
3D structure databases
ProteinModelPortal	Q9M4S8.
SMR	Q9M4S8. Positions 64-306.
ModBase	Search...
Proteomic databases
PRIDE	Q9M4S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIC_FRAAN

Accession

Primary (citable) accession number: Q9M4S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	May 31, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program