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Protein

Meiotic recombination protein SPO11-1

Gene

SPO11-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763).4 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Nucleophile1 Publication
Metal bindingi189 – 1891MagnesiumBy similarity
Metal bindingi241 – 2411MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • chiasma assembly Source: TAIR
  • DNA catabolic process, endonucleolytic Source: InterPro
  • meiotic DNA double-strand break formation Source: TAIR
  • reciprocal meiotic recombination Source: TAIR
  • synapsis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Isomerase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:GQT-2289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11-1 (EC:5.99.1.3Curated)
Short name:
AtSPO11-1
Gene namesi
Name:SPO11-1
Ordered Locus Names:At3g13170
ORF Names:MJG19.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G13170.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: InterPro
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants show a semi-sterile phenotype and a drastic decrease of meiotic recombination, indicating that SPO11-2 and SPO11-3 are not functionally redundant. SPO11-1 and SPO11-2 are both required for double-strand breaks induction.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi103 – 1031Y → F: Loss of double-strand breaks induction. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Meiotic recombination protein SPO11-1PRO_0000346110Add
BLAST

Proteomic databases

PaxDbiQ9M4A2.
PRIDEiQ9M4A2.

Expressioni

Tissue specificityi

Expressed in shoots, young seedlings, flowers and reproductive tissues. Not found in roots or rosette leaves.1 Publication

Developmental stagei

Accumulates in early G2 phase and then disappears over a period of between 1 and 5 hours post-S phase.1 Publication

Gene expression databases

GenevisibleiQ9M4A2. AT.

Interactioni

Subunit structurei

Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2 MTOPVIB chains (Probable). Interacts with MTOPVIB (PubMed:26917763). May form a heterodimer with SPO11-2. Interacts with PRD1 (PubMed:17762870). Does not interact with TOP6B (PubMed:11410368).3 Publications

Protein-protein interaction databases

IntActiQ9M4A2. 1 interaction.
MINTiMINT-5222860.
STRINGi3702.AT3G13170.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M4A2.
SMRiQ9M4A2. Positions 39-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000225930.
KOiK10878.
OMAiLANDMFC.
OrthoDBiEOG09360E5O.
PhylomeDBiQ9M4A2.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013048. Meiotic_Spo11.
IPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01551. SPO11HOMOLOG.
PR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9M4A2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKFAISES TNLLQRIKDF TQSVVVDLAE GRSPKISINQ FRNYCMNPEA
60 70 80 90 100
DCLCSSDKPK GQEIFTLKKE PQTYRIDMLL RVLLIVQQLL QENRHASKRD
110 120 130 140 150
IYYMHPSAFK AQSIVDRAIG DICILFQCSR YNLNVVSVGN GLVMGWLKFR
160 170 180 190 200
EAGRKFDCLN SLNTAYPVPV LVEEVEDIVS LAEYILVVEK ETVFQRLAND
210 220 230 240 250
MFCKTNRCIV ITGRGYPDVS TRRFLRLLME KLHLPVHCLV DCDPYGFEIL
260 270 280 290 300
ATYRFGSMQM AYDIESLRAP DMKWLGAFPS DSEVYSVPKQ CLLPLTEEDK
310 320 330 340 350
KRTEAMLLRC YLKREMPQWR LELETMLKRG VKFEIEALSV HSLSFLSEVY
360
IPSKIRREVS SP
Length:362
Mass (Da):41,804
Last modified:October 1, 2000 - v1
Checksum:iAE82942C4F456770
GO

Sequence cautioni

The sequence BAB01408 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51F → I in AAK21002 (PubMed:11157765).Curated
Sequence conflicti10 – 101S → T in AAK21002 (PubMed:11157765).Curated
Sequence conflicti26 – 261V → E in AAK21002 (PubMed:11157765).Curated
Sequence conflicti39 – 391N → S in AAK21002 (PubMed:11157765).Curated
Sequence conflicti65 – 651F → L in AAK21002 (PubMed:11157765).Curated
Sequence conflicti349 – 3491V → I in AAK21002 (PubMed:11157765).Curated
Sequence conflicti359 – 3591V → L in AAK21002 (PubMed:11157765).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251989 mRNA. Translation: CAB81544.1.
AF302928 Genomic DNA. Translation: AAK21002.1.
AP000375 Genomic DNA. Translation: BAB01408.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75304.1.
BT028955 mRNA. Translation: ABI49502.1.
PIRiT52652.
RefSeqiNP_187923.1. NM_112156.1. [Q9M4A2-1]
UniGeneiAt.48652.

Genome annotation databases

EnsemblPlantsiAT3G13170.1; AT3G13170.1; AT3G13170. [Q9M4A2-1]
GeneIDi820506.
KEGGiath:AT3G13170.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251989 mRNA. Translation: CAB81544.1.
AF302928 Genomic DNA. Translation: AAK21002.1.
AP000375 Genomic DNA. Translation: BAB01408.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75304.1.
BT028955 mRNA. Translation: ABI49502.1.
PIRiT52652.
RefSeqiNP_187923.1. NM_112156.1. [Q9M4A2-1]
UniGeneiAt.48652.

3D structure databases

ProteinModelPortaliQ9M4A2.
SMRiQ9M4A2. Positions 39-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9M4A2. 1 interaction.
MINTiMINT-5222860.
STRINGi3702.AT3G13170.1.

Proteomic databases

PaxDbiQ9M4A2.
PRIDEiQ9M4A2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G13170.1; AT3G13170.1; AT3G13170. [Q9M4A2-1]
GeneIDi820506.
KEGGiath:AT3G13170.

Organism-specific databases

TAIRiAT3G13170.

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000225930.
KOiK10878.
OMAiLANDMFC.
OrthoDBiEOG09360E5O.
PhylomeDBiQ9M4A2.

Enzyme and pathway databases

BioCyciARA:GQT-2289-MONOMER.

Miscellaneous databases

PROiQ9M4A2.

Gene expression databases

GenevisibleiQ9M4A2. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013048. Meiotic_Spo11.
IPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01551. SPO11HOMOLOG.
PR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPO11_ARATH
AccessioniPrimary (citable) accession number: Q9M4A2
Secondary accession number(s): Q9C5S3, Q9LK56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.