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Protein

Meiotic recombination protein SPO11-1

Gene

SPO11-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763).4 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103Nucleophile1 Publication1
Metal bindingi189MagnesiumBy similarity1
Metal bindingi241MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chiasma assembly Source: TAIR
  • DNA catabolic process, endonucleolytic Source: InterPro
  • double-strand break repair via homologous recombination Source: EnsemblPlants
  • meiotic DNA double-strand break formation Source: TAIR
  • reciprocal meiotic recombination Source: TAIR
  • synapsis Source: TAIR

Keywordsi

Molecular functionDNA-binding, Hydrolase, Isomerase
Biological processMeiosis
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11-1 (EC:5.99.1.3Curated)
Short name:
AtSPO11-1
Gene namesi
Name:SPO11-1
Ordered Locus Names:At3g13170
ORF Names:MJG19.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G13170.
TAIRilocus:2090044. AT3G13170.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants show a semi-sterile phenotype and a drastic decrease of meiotic recombination, indicating that SPO11-2 and SPO11-3 are not functionally redundant. SPO11-1 and SPO11-2 are both required for double-strand breaks induction.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103Y → F: Loss of double-strand breaks induction. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003461101 – 362Meiotic recombination protein SPO11-1Add BLAST362

Proteomic databases

PaxDbiQ9M4A2.

Expressioni

Tissue specificityi

Expressed in shoots, young seedlings, flowers and reproductive tissues. Not found in roots or rosette leaves.1 Publication

Developmental stagei

Accumulates in early G2 phase and then disappears over a period of between 1 and 5 hours post-S phase.1 Publication

Gene expression databases

ExpressionAtlasiQ9M4A2. baseline and differential.
GenevisibleiQ9M4A2. AT.

Interactioni

Subunit structurei

Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2 MTOPVIB chains (Probable). Interacts with MTOPVIB (PubMed:26917763). May form a heterodimer with SPO11-2. Interacts with PRD1 (PubMed:17762870). Does not interact with TOP6B (PubMed:11410368).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-62004N.
IntActiQ9M4A2. 3 interactors.
MINTiMINT-5222860.
STRINGi3702.AT3G13170.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M4A2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000225930.
KOiK10878.
OMAiCLPDCCL.
OrthoDBiEOG09360E5O.
PhylomeDBiQ9M4A2.

Family and domain databases

CDDicd00223. TOPRIM_TopoIIB_SPO. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR013048. Meiotic_Spo11.
IPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR036078. Spo11/TopoVI_A_sf.
IPR034136. TOPRIM_Topo6A/Spo11.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiView protein in Pfam
PF04406. TP6A_N. 1 hit.
PRINTSiPR01551. SPO11HOMOLOG.
PR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9M4A2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGKFAISES TNLLQRIKDF TQSVVVDLAE GRSPKISINQ FRNYCMNPEA
60 70 80 90 100
DCLCSSDKPK GQEIFTLKKE PQTYRIDMLL RVLLIVQQLL QENRHASKRD
110 120 130 140 150
IYYMHPSAFK AQSIVDRAIG DICILFQCSR YNLNVVSVGN GLVMGWLKFR
160 170 180 190 200
EAGRKFDCLN SLNTAYPVPV LVEEVEDIVS LAEYILVVEK ETVFQRLAND
210 220 230 240 250
MFCKTNRCIV ITGRGYPDVS TRRFLRLLME KLHLPVHCLV DCDPYGFEIL
260 270 280 290 300
ATYRFGSMQM AYDIESLRAP DMKWLGAFPS DSEVYSVPKQ CLLPLTEEDK
310 320 330 340 350
KRTEAMLLRC YLKREMPQWR LELETMLKRG VKFEIEALSV HSLSFLSEVY
360
IPSKIRREVS SP
Length:362
Mass (Da):41,804
Last modified:October 1, 2000 - v1
Checksum:iAE82942C4F456770
GO

Sequence cautioni

Q9M4A2: The sequence BAB01408 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5F → I in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti10S → T in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti26V → E in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti39N → S in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti65F → L in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti349V → I in AAK21002 (PubMed:11157765).Curated1
Sequence conflicti359V → L in AAK21002 (PubMed:11157765).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251989 mRNA. Translation: CAB81544.1.
AF302928 Genomic DNA. Translation: AAK21002.1.
AP000375 Genomic DNA. Translation: BAB01408.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75304.1.
BT028955 mRNA. Translation: ABI49502.1.
PIRiT52652.
RefSeqiNP_187923.1. NM_112156.2. [Q9M4A2-1]
UniGeneiAt.48652.

Genome annotation databases

EnsemblPlantsiAT3G13170.1; AT3G13170.1; AT3G13170. [Q9M4A2-1]
GeneIDi820506.
GrameneiAT3G13170.1; AT3G13170.1; AT3G13170.
KEGGiath:AT3G13170.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPO11_ARATH
AccessioniPrimary (citable) accession number: Q9M4A2
Secondary accession number(s): Q9C5S3, Q9LK56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families