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Protein

Meiotic recombination protein SPO11-2

Gene

SPO11-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:17018031, PubMed:17965269, PubMed:26917763).3 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241Nucleophile1 Publication
Metal bindingi217 – 2171MagnesiumBy similarity
Metal bindingi270 – 2701MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • chiasma assembly Source: TAIR
  • DNA catabolic process, endonucleolytic Source: InterPro
  • embryo sac development Source: TAIR
  • meiotic nuclear division Source: TAIR
  • pollen development Source: TAIR
  • seed development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Isomerase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:GQT-2072-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11-2 (EC:5.99.1.3Curated)
Short name:
AtSPO11-2
Gene namesi
Name:SPO11-2
Ordered Locus Names:At1g63990
ORF Names:F22C12.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G63990.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants show a semi-sterile phenotype and a drastic decrease of meiotic recombination, indicating that SPO11-1 and SPO11-3 are not functionally redundant. SPO11-1 and SPO11-2 are both required for double-strand breaks induction.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi124 – 1241Y → F: Loss of double-strand breaks induction. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Meiotic recombination protein SPO11-2PRO_0000346111Add
BLAST

Proteomic databases

PaxDbiQ9M4A1.
PRIDEiQ9M4A1.

Expressioni

Tissue specificityi

Very low expression in flowers and shoots.1 Publication

Gene expression databases

GenevisibleiQ9M4A1. AT.

Interactioni

Subunit structurei

Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2 MTOPVIB chains (Probable). Interacts with MTOPVIB (PubMed:26917763). May form a heterodimer with SPO11-1. Interacts with PRD1 (PubMed:17762870). Does not interact with TOP6B (PubMed:11410368).3 Publications

Protein-protein interaction databases

IntActiQ9M4A1. 1 interaction.
STRINGi3702.AT1G63990.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M4A1.
SMRiQ9M4A1. Positions 94-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000084591.
InParanoidiQ9M4A1.
KOiK10878.
OMAiRSLMRPN.
PhylomeDBiQ9M4A1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M4A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEESSGLSSM KFFSDQHLSY ADILLPHEAR ARIEVSVLNL LRILNSPDPA
60 70 80 90 100
ISDLSLINRK RSNSCINKGI LTDVSYIFLS TSFTKSSLTN AKTAKAFVRV
110 120 130 140 150
WKVMEICFQI LLQEKRVTQR ELFYKLLCDS PDYFSSQIEV NRSVQDVVAL
160 170 180 190 200
LRCSRYSLGI MASSRGLVAG RLFLQEPGKE AVDCSACGSS GFAITGDLNL
210 220 230 240 250
LDNTIMRTDA RYIIIVEKHA IFHRLVEDRV FNHIPCVFIT AKGYPDIATR
260 270 280 290 300
FFLHRMSTTF PDLPILVLVD WNPAGLAILC TFKFGSIGMG LEAYRYACNV
310 320 330 340 350
KWIGLRGDDL NLIPEESLVP LKPKDSQIAK SLLSSKILQE NYIEELSLMV
360 370 380
QTGKRAEIEA LYCHGYNYLG KYIATKIVQG KYI
Length:383
Mass (Da):43,126
Last modified:October 1, 2000 - v1
Checksum:i08AB5FA102A1F189
GO

Sequence cautioni

The sequence AAF24569.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251990 mRNA. Translation: CAB81545.1.
AC007764 Genomic DNA. Translation: AAF24569.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34178.1.
DQ446392 mRNA. Translation: ABE65738.1.
PIRiA96665.
T52651.
RefSeqiNP_176582.2. NM_105072.2.
UniGeneiAt.48342.

Genome annotation databases

EnsemblPlantsiAT1G63990.1; AT1G63990.1; AT1G63990.
GeneIDi842702.
GrameneiAT1G63990.1; AT1G63990.1; AT1G63990.
KEGGiath:AT1G63990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251990 mRNA. Translation: CAB81545.1.
AC007764 Genomic DNA. Translation: AAF24569.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34178.1.
DQ446392 mRNA. Translation: ABE65738.1.
PIRiA96665.
T52651.
RefSeqiNP_176582.2. NM_105072.2.
UniGeneiAt.48342.

3D structure databases

ProteinModelPortaliQ9M4A1.
SMRiQ9M4A1. Positions 94-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9M4A1. 1 interaction.
STRINGi3702.AT1G63990.1.

Proteomic databases

PaxDbiQ9M4A1.
PRIDEiQ9M4A1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G63990.1; AT1G63990.1; AT1G63990.
GeneIDi842702.
GrameneiAT1G63990.1; AT1G63990.1; AT1G63990.
KEGGiath:AT1G63990.

Organism-specific databases

TAIRiAT1G63990.

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000084591.
InParanoidiQ9M4A1.
KOiK10878.
OMAiRSLMRPN.
PhylomeDBiQ9M4A1.

Enzyme and pathway databases

BioCyciARA:GQT-2072-MONOMER.

Miscellaneous databases

PROiQ9M4A1.

Gene expression databases

GenevisibleiQ9M4A1. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana."
    Hartung F., Puchta H.
    Nucleic Acids Res. 28:1548-1554(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Flower.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Molecular characterization of homologues of both subunits A (SPO11) and B of the archaebacterial topoisomerase 6 in plants."
    Hartung F., Puchta H.
    Gene 271:81-86(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF INTERACTION WITH TOP6B.
    Strain: cv. Columbia.
    Tissue: Flower.
  6. Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  7. "AtPRD1 is required for meiotic double strand break formation in Arabidopsis thaliana."
    De Muyt A., Vezon D., Gendrot G., Gallois J.-L., Stevens R., Grelon M.
    EMBO J. 26:4126-4137(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRD1.
  8. "The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis."
    Hartung F., Wurz-Wildersinn R., Fuchs J., Schubert I., Suer S., Puchta H.
    Plant Cell 19:3090-3099(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, ACTIVE SITE, MUTAGENESIS OF TYR-124.
  9. "A DNA topoisomerase VI-like complex initiates meiotic recombination."
    Vrielynck N., Chambon A., Vezon D., Pereira L., Chelysheva L., De Muyt A., Mezard C., Mayer C., Grelon M.
    Science 351:939-943(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MTOPVIB.

Entry informationi

Entry nameiSPO12_ARATH
AccessioniPrimary (citable) accession number: Q9M4A1
Secondary accession number(s): Q9SH50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.