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Protein

Meiotic recombination protein SPO11-2

Gene

SPO11-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:17018031, PubMed:17965269, PubMed:26917763).3 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Nucleophile1 Publication1
Metal bindingi217MagnesiumBy similarity1
Metal bindingi270MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chiasma assembly Source: TAIR
  • DNA catabolic process, endonucleolytic Source: InterPro
  • embryo sac development Source: TAIR
  • meiotic cell cycle Source: TAIR
  • meiotic DNA double-strand break processing Source: GO_Central
  • pollen development Source: TAIR
  • seed development Source: TAIR

Keywordsi

Molecular functionDNA-binding, Hydrolase, Isomerase
Biological processMeiosis
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein SPO11-2 (EC:5.99.1.3Curated)
Short name:
AtSPO11-2
Gene namesi
Name:SPO11-2
Ordered Locus Names:At1g63990
ORF Names:F22C12.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G63990
TAIRilocus:2024598 AT1G63990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants show a semi-sterile phenotype and a drastic decrease of meiotic recombination, indicating that SPO11-1 and SPO11-3 are not functionally redundant. SPO11-1 and SPO11-2 are both required for double-strand breaks induction.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124Y → F: Loss of double-strand breaks induction. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003461111 – 383Meiotic recombination protein SPO11-2Add BLAST383

Proteomic databases

PaxDbiQ9M4A1

Expressioni

Tissue specificityi

Very low expression in flowers and shoots.1 Publication

Gene expression databases

ExpressionAtlasiQ9M4A1 baseline and differential
GenevisibleiQ9M4A1 AT

Interactioni

Subunit structurei

Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2 MTOPVIB chains (Probable). Interacts with MTOPVIB (PubMed:26917763). May form a heterodimer with SPO11-1. Interacts with PRD1 (PubMed:17762870). Does not interact with TOP6B (PubMed:11410368).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-40433N
IntActiQ9M4A1, 3 interactors
STRINGi3702.AT1G63990.1

Structurei

3D structure databases

ProteinModelPortaliQ9M4A1
SMRiQ9M4A1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

eggNOGiKOG2795 Eukaryota
COG1697 LUCA
HOGENOMiHOG000084591
InParanoidiQ9M4A1
KOiK10878
OMAiCNVKWIG
OrthoDBiEOG09360F8Q
PhylomeDBiQ9M4A1

Family and domain databases

CDDicd00223 TOPRIM_TopoIIB_SPO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR002815 Spo11/TopoVI_A
IPR013049 Spo11/TopoVI_A_N
IPR036078 Spo11/TopoVI_A_sf
IPR034136 TOPRIM_Topo6A/Spo11
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10848 PTHR10848, 1 hit
PfamiView protein in Pfam
PF04406 TP6A_N, 1 hit
PRINTSiPR01550 TOP6AFAMILY
SUPFAMiSSF56726 SSF56726, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M4A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEESSGLSSM KFFSDQHLSY ADILLPHEAR ARIEVSVLNL LRILNSPDPA
60 70 80 90 100
ISDLSLINRK RSNSCINKGI LTDVSYIFLS TSFTKSSLTN AKTAKAFVRV
110 120 130 140 150
WKVMEICFQI LLQEKRVTQR ELFYKLLCDS PDYFSSQIEV NRSVQDVVAL
160 170 180 190 200
LRCSRYSLGI MASSRGLVAG RLFLQEPGKE AVDCSACGSS GFAITGDLNL
210 220 230 240 250
LDNTIMRTDA RYIIIVEKHA IFHRLVEDRV FNHIPCVFIT AKGYPDIATR
260 270 280 290 300
FFLHRMSTTF PDLPILVLVD WNPAGLAILC TFKFGSIGMG LEAYRYACNV
310 320 330 340 350
KWIGLRGDDL NLIPEESLVP LKPKDSQIAK SLLSSKILQE NYIEELSLMV
360 370 380
QTGKRAEIEA LYCHGYNYLG KYIATKIVQG KYI
Length:383
Mass (Da):43,126
Last modified:October 1, 2000 - v1
Checksum:i08AB5FA102A1F189
GO

Sequence cautioni

The sequence AAF24569 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251990 mRNA Translation: CAB81545.1
AC007764 Genomic DNA Translation: AAF24569.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34178.1
DQ446392 mRNA Translation: ABE65738.1
PIRiA96665
T52651
RefSeqiNP_176582.2, NM_105072.3
UniGeneiAt.48342

Genome annotation databases

EnsemblPlantsiAT1G63990.1; AT1G63990.1; AT1G63990
GeneIDi842702
GrameneiAT1G63990.1; AT1G63990.1; AT1G63990
KEGGiath:AT1G63990

Similar proteinsi

Entry informationi

Entry nameiSPO12_ARATH
AccessioniPrimary (citable) accession number: Q9M4A1
Secondary accession number(s): Q9SH50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health