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Protein

Branched-chain-amino-acid aminotransferase 2, chloroplastic

Gene

BCAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and 2-oxoglutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala.1 Publication

Miscellaneous

Branched-chain amino acids are synthesized in chloroplasts, whereas the degradation takes place in mitochondria.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.1 Publication
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.1 Publication

Cofactori

Kineticsi

  1. KM=0.71 mM for L-leucine1 Publication

    Pathwayi: L-isoleucine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810), Acetolactate synthase (AXX17_At3g42690)
    2. Ketol-acid reductoisomerase (AXX17_At3g53150), Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
    3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
    4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
    This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-leucine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. 2-isopropylmalate synthase 2, chloroplastic (IPMS2), 2-isopropylmalate synthase 1, chloroplastic (IPMS1)
    2. 3-isopropylmalate dehydratase small subunit 1 (IPMI2), 3-isopropylmalate dehydratase small subunit 3 (At2g43090), 3-isopropylmalate dehydratase small subunit 2 (IPMI1), 3-isopropylmalate dehydratase large subunit, chloroplastic (IIL1)
    3. 3-isopropylmalate dehydrogenase (At1g31180/F28K20_14), 3-isopropylmalate dehydrogenase (IMD1), 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3), 3-isopropylmalate dehydrogenase 2, chloroplastic (IMDH2), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (AXX17_At1g31810), 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (AXX17_At5g13660), 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase (IMD3), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase 1, chloroplastic (IMDH1)
    4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
    This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

    Pathwayi: L-valine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes L-valine from pyruvate.
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810), Acetolactate synthase (AXX17_At3g42690)
    2. Ketol-acid reductoisomerase (AXX17_At3g53150), Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
    3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
    4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
    This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminotransferase, Transferase
    Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.
    UniPathwayiUPA00047; UER00058.
    UPA00048; UER00073.
    UPA00049; UER00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Branched-chain-amino-acid aminotransferase 2, chloroplastic (EC:2.6.1.42)
    Short name:
    Atbcat-2
    Gene namesi
    Name:BCAT2
    Ordered Locus Names:At1g10070
    ORF Names:T27I1.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G10070.
    TAIRilocus:2201921. AT1G10070.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 22ChloroplastSequence analysisAdd BLAST22
    ChainiPRO_000000127623 – 388Branched-chain-amino-acid aminotransferase 2, chloroplasticAdd BLAST366

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei235N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiQ9M439.

    PTM databases

    iPTMnetiQ9M439.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ9M439. baseline and differential.
    GenevisibleiQ9M439. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi22783. 8 interactors.
    IntActiQ9M439. 2 interactors.
    STRINGi3702.AT1G10070.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M439.
    SMRiQ9M439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG0975. Eukaryota.
    COG0115. LUCA.
    HOGENOMiHOG000276704.
    InParanoidiQ9M439.
    KOiK00826.
    OMAiKGWGQPR.
    OrthoDBiEOG09360CSJ.
    PhylomeDBiQ9M439.

    Family and domain databases

    CDDicd01557. BCAT_beta_family. 1 hit.
    InterProiView protein in InterPro
    IPR001544. Aminotrans_IV.
    IPR018300. Aminotrans_IV_CS.
    IPR005786. B_amino_transII.
    IPR033939. BCAT_family.
    PfamiView protein in Pfam
    PF01063. Aminotran_4. 1 hit.
    PIRSFiPIRSF006468. BCAT1. 1 hit.
    SUPFAMiSSF56752. SSF56752. 1 hit.
    TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
    PROSITEiView protein in PROSITE
    PS00770. AA_TRANSFER_CLASS_4. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9M439-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MIKTITSLRK TLVLPLHLHI RTLQTFAKYN AQAASALREE RKKPLYQNGD
    60 70 80 90 100
    DVYADLDWDN LGFGLNPADY MYVMKCSKDG EFTQGELSPY GNIQLSPSAG
    110 120 130 140 150
    VLNYGQAIYE GTKAYRKENG KLLLFRPDHN AIRMKLGAER MLMPSPSVDQ
    160 170 180 190 200
    FVNAVKQTAL ANKRWVPPAG KGTLYIRPLL MGSGPILGLG PAPEYTFIVY
    210 220 230 240 250
    ASPVGNYFKE GMAALNLYVE EEYVRAAPGG AGGVKSITNY APVLKALSRA
    260 270 280 290 300
    KSRGFSDVLY LDSVKKKYLE EASSCNVFVV KGRTISTPAT NGTILEGITR
    310 320 330 340 350
    KSVMEIASDQ GYQVVEKAVH VDEVMDADEV FCTGTAVVVA PVGTITYQEK
    360 370 380
    RVEYKTGDES VCQKLRSVLV GIQTGLIEDN KGWVTDIN
    Length:388
    Mass (Da):42,591
    Last modified:October 1, 2000 - v1
    Checksum:iC457DBADF362DCF0
    GO

    Sequence cautioni

    The sequence AAC34333 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ271731 mRNA. Translation: CAB93128.1.
    AC004122 Genomic DNA. Translation: AAC34333.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28537.1.
    CP002684 Genomic DNA. Translation: AEE28538.1.
    AY051038 mRNA. Translation: AAK93715.1.
    AF370135 mRNA. Translation: AAK43950.1.
    PIRiT00626.
    RefSeqiNP_001031015.1. NM_001035938.2. [Q9M439-1]
    NP_001031016.2. NM_001035939.3.
    NP_172478.1. NM_100881.3. [Q9M439-1]
    UniGeneiAt.18974.
    At.48176.

    Genome annotation databases

    EnsemblPlantsiAT1G10070.1; AT1G10070.1; AT1G10070. [Q9M439-1]
    AT1G10070.2; AT1G10070.2; AT1G10070. [Q9M439-1]
    GeneIDi837543.
    GrameneiAT1G10070.1; AT1G10070.1; AT1G10070.
    AT1G10070.2; AT1G10070.2; AT1G10070.
    KEGGiath:AT1G10070.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiBCAT2_ARATH
    AccessioniPrimary (citable) accession number: Q9M439
    Secondary accession number(s): O80598
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: October 1, 2000
    Last modified: August 30, 2017
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families