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Protein

Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic

Gene

HMA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in cadmium/zinc transport.Curated

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4534-aspartylphosphate intermediateBy similarity1
Metal bindingi682MagnesiumBy similarity1
Metal bindingi686MagnesiumBy similarity1

GO - Molecular functioni

  • ATPase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • cadmium-exporting ATPase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW
  • zinc-exporting ATPase activity Source: UniProtKB-EC
  • zinc-transporting ATPase activity Source: TAIR

GO - Biological processi

  • cellular copper ion homeostasis Source: TAIR
  • response to light intensity Source: TAIR
  • zinc ion homeostasis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G37270-MONOMER.

Protein family/group databases

TCDBi3.A.3.6.6. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic (EC:3.6.3.3, EC:3.6.3.5)
Alternative name(s):
Protein HEAVY METAL ATPASE 1
Gene namesi
Name:HMA1
Ordered Locus Names:At4g37270
ORF Names:AP22.4, C7A10.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G37270.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 122StromalSequence analysisAdd BLAST105
Transmembranei123 – 144HelicalSequence analysisAdd BLAST22
Topological domaini145 – 153LumenalSequence analysis9
Transmembranei154 – 173HelicalSequence analysisAdd BLAST20
Topological domaini174 – 180StromalSequence analysis7
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202LumenalSequence analysis1
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 361StromalSequence analysisAdd BLAST138
Transmembranei362 – 384HelicalSequence analysisAdd BLAST23
Topological domaini385 – 398LumenalSequence analysisAdd BLAST14
Transmembranei399 – 416HelicalSequence analysisAdd BLAST18
Topological domaini417 – 737StromalSequence analysisAdd BLAST321
Transmembranei738 – 757HelicalSequence analysisAdd BLAST20
Topological domaini758 – 762LumenalSequence analysis5
Transmembranei763 – 781HelicalSequence analysisAdd BLAST19
Topological domaini782 – 819StromalSequence analysisAdd BLAST38

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast inner membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17ChloroplastSequence analysisAdd BLAST17
ChainiPRO_000004639818 – 819Probable cadmium/zinc-transporting ATPase HMA1, chloroplasticAdd BLAST802

Proteomic databases

PaxDbiQ9M3H5.
PRIDEiQ9M3H5.

Expressioni

Gene expression databases

GenevisibleiQ9M3H5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M3H5.
SMRiQ9M3H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi79 – 89Poly-HisAdd BLAST11
Compositional biasi809 – 817Poly-Ser9

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiQ9M3H5.
OMAiFKSEDES.
OrthoDBiEOG093603VO.
PhylomeDBiQ9M3H5.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M3H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPATLTRSS SLTRFPYRRG LSTLRLARVN SFSILPPKTL LRQKPLRISA
60 70 80 90 100
SLNLPPRSIR LRAVEDHHHD HHHDDEQDHH NHHHHHHQHG CCSVELKAES
110 120 130 140 150
KPQKMLFGFA KAIGWVRLAN YLREHLHLCC SAAAMFLAAA VCPYLAPEPY
160 170 180 190 200
IKSLQNAFMI VGFPLVGVSA SLDALMDIAG GKVNIHVLMA LAAFASVFMG
210 220 230 240 250
NALEGGLLLA MFNLAHIAEE FFTSRSMVDV KELKESNPDS ALLIEVHNGN
260 270 280 290 300
VPNISDLSYK SVPVHSVEVG SYVLVGTGEI VPVDCEVYQG SATITIEHLT
310 320 330 340 350
GEVKPLEAKA GDRVPGGARN LDGRMIVKAT KAWNDSTLNK IVQLTEEAHS
360 370 380 390 400
NKPKLQRWLD EFGENYSKVV VVLSLAIAFL GPFLFKWPFL STAACRGSVY
410 420 430 440 450
RALGLMVAAS PCALAVAPLA YATAISSCAR KGILLKGAQV LDALASCHTI
460 470 480 490 500
AFDKTGTLTT GGLTCKAIEP IYGHQGGTNS SVITCCIPNC EKEALAVAAA
510 520 530 540 550
MEKGTTHPIG RAVVDHSVGK DLPSIFVESF EYFPGRGLTA TVNGVKTVAE
560 570 580 590 600
ESRLRKASLG SIEFITSLFK SEDESKQIKD AVNASSYGKD FVHAALSVDQ
610 620 630 640 650
KVTLIHLEDQ PRPGVSGVIA ELKSWARLRV MMLTGDHDSS AWRVANAVGI
660 670 680 690 700
TEVYCNLKPE DKLNHVKNIA REAGGGLIMV GEGINDAPAL AAATVGIVLA
710 720 730 740 750
QRASATAIAV ADILLLRDNI TGVPFCVAKS RQTTSLVKQN VALALTSIFL
760 770 780 790 800
AALPSVLGFV PLWLTVLLHE GGTLLVCLNS VRGLNDPSWS WKQDIVHLIN
810
KLRSQEPTSS SSNSLSSAH
Length:819
Mass (Da):88,189
Last modified:January 11, 2001 - v2
Checksum:iF281EA8F33C0ED52
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti53N → S in strain: cv. Landsberg erecta. 1
Natural varianti105M → V in strain: cv. Landsberg erecta. 1
Natural varianti659P → S in strain: cv. Landsberg erecta. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ400906 mRNA. Translation: CAB90352.1.
AJ872069 mRNA. Translation: CAI43274.1.
Z99707 Genomic DNA. Translation: CAB16773.1.
AL161591 Genomic DNA. Translation: CAB80393.1.
CP002687 Genomic DNA. Translation: AEE86775.1.
PIRiD85440.
RefSeqiNP_195444.1. NM_119890.7.
UniGeneiAt.56989.
At.74483.

Genome annotation databases

EnsemblPlantsiAT4G37270.1; AT4G37270.1; AT4G37270.
GeneIDi829881.
GrameneiAT4G37270.1; AT4G37270.1; AT4G37270.
KEGGiath:AT4G37270.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ400906 mRNA. Translation: CAB90352.1.
AJ872069 mRNA. Translation: CAI43274.1.
Z99707 Genomic DNA. Translation: CAB16773.1.
AL161591 Genomic DNA. Translation: CAB80393.1.
CP002687 Genomic DNA. Translation: AEE86775.1.
PIRiD85440.
RefSeqiNP_195444.1. NM_119890.7.
UniGeneiAt.56989.
At.74483.

3D structure databases

ProteinModelPortaliQ9M3H5.
SMRiQ9M3H5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G37270.1.

Protein family/group databases

TCDBi3.A.3.6.6. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiQ9M3H5.
PRIDEiQ9M3H5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37270.1; AT4G37270.1; AT4G37270.
GeneIDi829881.
GrameneiAT4G37270.1; AT4G37270.1; AT4G37270.
KEGGiath:AT4G37270.

Organism-specific databases

TAIRiAT4G37270.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiQ9M3H5.
OMAiFKSEDES.
OrthoDBiEOG093603VO.
PhylomeDBiQ9M3H5.

Enzyme and pathway databases

BioCyciARA:AT4G37270-MONOMER.

Miscellaneous databases

PROiQ9M3H5.

Gene expression databases

GenevisibleiQ9M3H5. AT.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMA1_ARATH
AccessioniPrimary (citable) accession number: Q9M3H5
Secondary accession number(s): Q5JZZ1, Q9SW66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.