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Protein

Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic

Gene

HMA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in cadmium/zinc transport.Curated

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4534-aspartylphosphate intermediateBy similarity1
Metal bindingi682MagnesiumBy similarity1
Metal bindingi686MagnesiumBy similarity1

GO - Molecular functioni

  • ATPase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • cadmium-exporting ATPase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW
  • zinc-exporting ATPase activity Source: UniProtKB-EC
  • zinc-transporting ATPase activity Source: TAIR

GO - Biological processi

  • cellular copper ion homeostasis Source: TAIR
  • response to light intensity Source: TAIR
  • zinc ion homeostasis Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G37270-MONOMER

Protein family/group databases

TCDBi3.A.3.6.6 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic (EC:3.6.3.3, EC:3.6.3.5)
Alternative name(s):
Protein HEAVY METAL ATPASE 1
Gene namesi
Name:HMA1
Ordered Locus Names:At4g37270
ORF Names:AP22.4, C7A10.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37270
TAIRilocus:2115020 AT4G37270

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 122StromalSequence analysisAdd BLAST105
Transmembranei123 – 144HelicalSequence analysisAdd BLAST22
Topological domaini145 – 153LumenalSequence analysis9
Transmembranei154 – 173HelicalSequence analysisAdd BLAST20
Topological domaini174 – 180StromalSequence analysis7
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202LumenalSequence analysis1
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 361StromalSequence analysisAdd BLAST138
Transmembranei362 – 384HelicalSequence analysisAdd BLAST23
Topological domaini385 – 398LumenalSequence analysisAdd BLAST14
Transmembranei399 – 416HelicalSequence analysisAdd BLAST18
Topological domaini417 – 737StromalSequence analysisAdd BLAST321
Transmembranei738 – 757HelicalSequence analysisAdd BLAST20
Topological domaini758 – 762LumenalSequence analysis5
Transmembranei763 – 781HelicalSequence analysisAdd BLAST19
Topological domaini782 – 819StromalSequence analysisAdd BLAST38

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17ChloroplastSequence analysisAdd BLAST17
ChainiPRO_000004639818 – 819Probable cadmium/zinc-transporting ATPase HMA1, chloroplasticAdd BLAST802

Proteomic databases

PaxDbiQ9M3H5
PRIDEiQ9M3H5

Expressioni

Gene expression databases

ExpressionAtlasiQ9M3H5 baseline and differential
GenevisibleiQ9M3H5 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37270.1

Structurei

3D structure databases

ProteinModelPortaliQ9M3H5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi79 – 89Poly-HisAdd BLAST11
Compositional biasi809 – 817Poly-Ser9

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207 Eukaryota
COG2217 LUCA
HOGENOMiHOG000250399
InParanoidiQ9M3H5
KOiK01534
OMAiRAVEDHH
OrthoDBiEOG093603VO
PhylomeDBiQ9M3H5

Family and domain databases

Gene3Di3.40.1110.101 hit
3.40.50.10002 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
SUPFAMiSSF56784 SSF56784, 3 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M3H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPATLTRSS SLTRFPYRRG LSTLRLARVN SFSILPPKTL LRQKPLRISA
60 70 80 90 100
SLNLPPRSIR LRAVEDHHHD HHHDDEQDHH NHHHHHHQHG CCSVELKAES
110 120 130 140 150
KPQKMLFGFA KAIGWVRLAN YLREHLHLCC SAAAMFLAAA VCPYLAPEPY
160 170 180 190 200
IKSLQNAFMI VGFPLVGVSA SLDALMDIAG GKVNIHVLMA LAAFASVFMG
210 220 230 240 250
NALEGGLLLA MFNLAHIAEE FFTSRSMVDV KELKESNPDS ALLIEVHNGN
260 270 280 290 300
VPNISDLSYK SVPVHSVEVG SYVLVGTGEI VPVDCEVYQG SATITIEHLT
310 320 330 340 350
GEVKPLEAKA GDRVPGGARN LDGRMIVKAT KAWNDSTLNK IVQLTEEAHS
360 370 380 390 400
NKPKLQRWLD EFGENYSKVV VVLSLAIAFL GPFLFKWPFL STAACRGSVY
410 420 430 440 450
RALGLMVAAS PCALAVAPLA YATAISSCAR KGILLKGAQV LDALASCHTI
460 470 480 490 500
AFDKTGTLTT GGLTCKAIEP IYGHQGGTNS SVITCCIPNC EKEALAVAAA
510 520 530 540 550
MEKGTTHPIG RAVVDHSVGK DLPSIFVESF EYFPGRGLTA TVNGVKTVAE
560 570 580 590 600
ESRLRKASLG SIEFITSLFK SEDESKQIKD AVNASSYGKD FVHAALSVDQ
610 620 630 640 650
KVTLIHLEDQ PRPGVSGVIA ELKSWARLRV MMLTGDHDSS AWRVANAVGI
660 670 680 690 700
TEVYCNLKPE DKLNHVKNIA REAGGGLIMV GEGINDAPAL AAATVGIVLA
710 720 730 740 750
QRASATAIAV ADILLLRDNI TGVPFCVAKS RQTTSLVKQN VALALTSIFL
760 770 780 790 800
AALPSVLGFV PLWLTVLLHE GGTLLVCLNS VRGLNDPSWS WKQDIVHLIN
810
KLRSQEPTSS SSNSLSSAH
Length:819
Mass (Da):88,189
Last modified:January 11, 2001 - v2
Checksum:iF281EA8F33C0ED52
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti53N → S in strain: cv. Landsberg erecta. 1
Natural varianti105M → V in strain: cv. Landsberg erecta. 1
Natural varianti659P → S in strain: cv. Landsberg erecta. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ400906 mRNA Translation: CAB90352.1
AJ872069 mRNA Translation: CAI43274.1
Z99707 Genomic DNA Translation: CAB16773.1
AL161591 Genomic DNA Translation: CAB80393.1
CP002687 Genomic DNA Translation: AEE86775.1
PIRiD85440
RefSeqiNP_195444.1, NM_119890.7
UniGeneiAt.56989
At.74483

Genome annotation databases

EnsemblPlantsiAT4G37270.1; AT4G37270.1; AT4G37270
GeneIDi829881
GrameneiAT4G37270.1; AT4G37270.1; AT4G37270
KEGGiath:AT4G37270

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHMA1_ARATH
AccessioniPrimary (citable) accession number: Q9M3H5
Secondary accession number(s): Q5JZZ1, Q9SW66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: April 25, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome