Reviewed, UniProtKB/Swiss-Prot Q9M3G7 (ATM_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATM
    EC=2.7.11.1
Alternative name(s):
    Ataxia telangiectasia mutated homolog
      Short name=AtATM

Gene names

Name:	ATM
Ordered Locus Names:	At3g48190
ORF Names:	T24C20.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	3856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR) or DNA replication stalling. Plays a central role in the perception and response to both stress-induced damage in somatic cells and developmentally programmed DNA damage during meiosis. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone variant H2AX to form H2AXS139ph at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Involved in transcriptional regulation of RAD51, PARP1, GR1, and LIG4 in response to DNA double strand breaks. Plays a dual role by activating the DNA damage response at dysfunctional telomeres and yet preventing this activation at functional telomeres. Not required for telomere length homeostasis. Ref.3 Ref.4 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus Probable.
Tissue specificity	Ubiquitously expressed at low levels with slightly higher levels in flower buds. Ref.1
Disruption phenotype	Plants show meiotic defects, hypersensitivity to double strand breaks-inducing agents, and an inability to properly induce IR-mediated transcription of several DNA repair genes. Ref.3
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain. Contains 1 PWWP domain.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair Meiosis
Cellular component	Chromosomal protein Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 3856	3856	Serine/threonine-protein kinase ATM		PRO_0000088843
Regions
	Domain	108 – 162	55	PWWP
	Domain	2727 – 3393	667	FAT
	Domain	3525 – 3773	249	PI3K/PI4K
	Domain	3824 – 3856	33	FATC
	Motif	3233 – 3249	17	Bipartite nuclear localization signal Potential
Experimental info
	Sequence conflict	3640	1	P → S in CAB86487. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9M3G7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 934D08DC3C67A180	FASTA	3,856	435,114
	10 20 30 40 50 60 MKLQNPDKKT LREGFSQESS VVALDSGVLA MSGLKCDGKF PVKDVLMEEG GDKVRKIQVS 70 80 90 100 110 120 GGNISLVVDF SGARTSSNNF FESNASCVNE NLVKGNGYRE DETQEFLVGN LVWVMTKYKK 130 140 150 160 170 180 WWPGEVVDFK ADAKESFMVR SIGQSHLVSW FASSKLKPFK ESFEQVLNQR NDNGFFDALQ 190 200 210 220 230 240 KAMSLLSNSL KLDMTCSCIA DGNGIVSAQN ITTRKNKPLI LREFSVDRLE PKEFVTQLKN 250 260 270 280 290 300 IAKCVLNAGV LESTVMQSQL SAFYTLFGHK QIPMAQLHEN EGRKSFTAKM SDSKFIGSPS 310 320 330 340 350 360 ICAGNSRKRF RKEWFRKFVS EVDNVSARDD LVNVPPSDLI SKLKLLAVGY NCSEETENIG 370 380 390 400 410 420 LFEWFFSKFR ISVYHDENAY KMQLANMAGF KDLMLATNAN RGTVQKTLKS KKIGKSKMEP 430 440 450 460 470 480 LNGVSVADTE QKTFELQISK KSNIESLNGV SVADTEQKTF ELQILEKSNI ESLNGVSTPN 490 500 510 520 530 540 IDHEASKSNN SGKTKINHII GHSNFPSSVA KVQLAKDFQD KLLVQAPDRK AMTADTLSRP 550 560 570 580 590 600 AAILVPDLNS GGNALGTAEF DHMQRPETLI QHNVCPQEEK TPRSTILNFQ VTAHQGVSGT 610 620 630 640 650 660 QFVSSQPTSY KHFTSADLFT YSGKKKRGRK RKNAEELPIV AHASATTGIP DLNGTNTEPT 670 680 690 700 710 720 LVLPQVEPTQ RRRRRKKEES PNGLTRGITI LFLKFSSQVS MPSRDDLTST FSAFGPLDSS 730 740 750 760 770 780 ETHVSEEFSG AQVAFVSSAD AIEAVKSLEK ANPFGETLVN FRLQQKLITV QRNIAPRMPV 790 800 810 820 830 840 ISHVSPVPKP NNIPTSMDAM RQNLLMMTAM LEKSGDSLSR ETKAKLKSEI TGLLEKDGVK 850 860 870 880 890 900 LLNTWLEGER SITFCRFLSQ NTAKLKLDEI PNAETWPFLV KLLLQCVSME VSGSKRRMPK 910 920 930 940 950 960 PTFAKTLRVV VQRTEETKFP GVQFPLLSMA KTLFTHVHDI LSNTPSFQSE YGTILRHLLE 970 980 990 1000 1010 1020 IKEYRFQMRK RTYSSLVLLY MERAETGFCE KNSGQHSQKE EAFRYILTLQ SLLENSPGDF 1030 1040 1050 1060 1070 1080 PDDLREEIVN GLIHIFSSVR DEGKLSRKLI ECVNTFLLKD GPNLGSLSLE IHNAVEQFVF 1090 1100 1110 1120 1130 1140 RCWLTTHDKN LKEILVSYGR LQLNLTRDSS ESSSLVEQLL DVVTRELDLG SSSSSASWGD 1150 1160 1170 1180 1190 1200 TTKDEKLGAL SSYQNSLVEL AAHVFYRACV NTSRPSLSEK RARRQHIAMR MVDALTEGKW 1210 1220 1230 1240 1250 1260 LWCAAFGCLV RNYCARINMD LLIYWFEAIC TNFQRLLEDA SMRRSYDGLL WTLRSLQGLS 1270 1280 1290 1300 1310 1320 SGLSLPDITM DISKSSASSS ELDRGWQSIW SSLIHGLATF SSMSVIVDAV LVLLGSIISS 1330 1340 1350 1360 1370 1380 NHITVKILPQ EVWDHQLFRH IPSEPALYFI ACYFSRMGCQ GNLQDDLHLR RNLLRAVCAP 1390 1400 1410 1420 1430 1440 LSWKVRLTLD ERMVQLLPAA AFSLCAGFKV SLPLPKEHLP TPSQWDVCEQ IDDVDRERNF 1450 1460 1470 1480 1490 1500 GLFECSVEAL TRICSNSSKI SGCQVPDVVQ LPLVLRDPLL HDMDIYFLSI IPEVKEKGPL 1510 1520 1530 1540 1550 1560 SDIFMGCALL CHFMHGSYIT RKGKGSSSFF LKACQYLLEG LDHAVESVSK SLNDLQRRGS 1570 1580 1590 1600 1610 1620 LGFGSDFNEK GSIIVSLRSF TQSPVFSNRR DQNLLGASYD FVIHSLENLL RSFAKVYEEY 1630 1640 1650 1660 1670 1680 TEHAWNTHSD TVPSKSLAPD SPEVGRIVDM DLDLAEDTKE RDIIAAGGKA VPGLPVSMGN 1690 1700 1710 1720 1730 1740 WKLGMVSLIS CFSPVLQFPT WDVLYNLLEK ESDPKVLENI LYHLCKLSCL TSIPKVDDLV 1750 1760 1770 1780 1790 1800 IFLDGMLSTQ VKMKRNCLNI VTALHVLLHT LSSSRRDSSG VEKNCGLSLK EAESFQVFVQ 1810 1820 1830 1840 1850 1860 LGAMVNKVSE FGLLGWFGRV KLINCICDLV LLNPQTGQTM IERLLLMLSD SDYRVRFVLA 1870 1880 1890 1900 1910 1920 RQIGILFQTW DGHEALFQDI CSSFGIKLVT SSKEKLVTAK DVLAVGPQPR QKMETVIITL 1930 1940 1950 1960 1970 1980 MHLAYHSENI ELQAVFMMCA VSAKDPCQRE LIIAALDNLS AQLHYPSRFK YLEELLGPIL 1990 2000 2010 2020 2030 2040 FHWIASGVSL AGLIETSQLF IPNAEPKYFI HFCSHWLLPA LLLHEDHTNL DWVAKMAGQP 2050 2060 2070 2080 2090 2100 VVVLVKENFV PIFSICMGLH CSKTSECDKG AMVLQNSILY VGETSENERD KLIKQNMVSI 2110 2120 2130 2140 2150 2160 VSFILSCASS SPEPPVPTFS RDTISLAVQT VVDGFLENTD YPKNAAITDR INIFRPDRVF 2170 2180 2190 2200 2210 2220 MFITEMHYRM SAACHHRHTR HHLAALEELT ILLGHRALVP SSLNYIFNLV GQFIGYPSLQ 2230 2240 2250 2260 2270 2280 DQCCSIASCL LDLFKSNPAK EIVSVLGDQL QFLVSKLVTC CIDAEADTKI SGAKSSQLVN 2290 2300 2310 2320 2330 2340 LLHKLVVSSD SSLNEDIRDL EPLPDLKYFQ VIRESHIRIC EAYSPRNHLL KVEHSTFLIY 2350 2360 2370 2380 2390 2400 IFLEILSLSN FLFLSCSTIQ QCSRRSNYLP PRFLSRSLQA LHNKLIASEV SQEDTNGETA 2410 2420 2430 2440 2450 2460 ETFWQSDDEI VNAVWTLVRV SASDEADSMR LLVSDFLSRI GIRDPHTVVF HLPGNLVSMH 2470 2480 2490 2500 2510 2520 GLQGFGHNTG SKVRSLTENG ISDETLITLL NFLKKYLLDD SVKIIDVTSQ TLRGILSTER 2530 2540 2550 2560 2570 2580 GQQALSSFDS CERALIEVHG RGVNLDIVEK ILLDSQKQFK AEKFSLETPE VWSTDNKNFD 2590 2600 2610 2620 2630 2640 RWICQLVYCM IALCEDVPIR LCQNIALLKA EISELLFPSV VVSLAGRIGM DINLHDLITS 2650 2660 2670 2680 2690 2700 QVKEHIFTDS NKLTKSKQVM LNTLNELRMC YVLERSIFSG QTKREKNSRS CSTAAKIRDV 2710 2720 2730 2740 2750 2760 ESGSNGMAAS ITTNWEKVYW LSIDYLVVAG SAVVCGAYLT ASMYVEYWCE EKFGNLSLGD 2770 2780 2790 2800 2810 2820 PDFSYHDKLP DHVEILVSAI TRINEPDSLY GVIHSNKLSA QIITFEHEGN WTRALEYYDL 2830 2840 2850 2860 2870 2880 QARSQKMVVP SSLSENLEVE QFQPTTSARH SVFGEGEVQR QPFKGLIRSL QQTGCMHVLD 2890 2900 2910 2920 2930 2940 LYCRGLTSRE GCFQYDPEFI ELQYEAAWRA GKWDFSLLYP QTHCQPLQHA KNNNYHESLH 2950 2960 2970 2980 2990 3000 CCLRALQEGD YDGFYGKLKD TKKELVLSIS RASEESTEFI YSTVVKLQIL HHLGLVWDLR 3010 3020 3030 3040 3050 3060 WTTSSHQSVH GYLVKQMACV DPVIPTMDQL SWLNKDWNSI ITQTQLHMTL LEPFIAFRRV 3070 3080 3090 3100 3110 3120 LLQILGCEKC TMQHLLQSAS LLRKGTRFSH AAASLHEFKF LCARSNGQQP VPDWLGKLEE 3130 3140 3150 3160 3170 3180 AKLLHAQGRH EVSISLANYI LHNYQLKEEA SDIYRVIGKW LAETRSSNSR TILEKYLRPA 3190 3200 3210 3220 3230 3240 VSLAEEQSSK ICKRLVDRQS QTWFHLAHYA DALFKSYEER LSSSEWQAAL RLRKHKTKEL 3250 3260 3270 3280 3290 3300 EVFIKRFKSS KKAEQSDYSL KIQDLQKQLT MDKEEAEKLQ VDRDNFLKLA LEGYKRCLEI 3310 3320 3330 3340 3350 3360 GDKYDVRVVF RQVSMWFSLA SQKNVIDNML STIKEVQSYK FIPLVYQIAS RLGSSKDESG 3370 3380 3390 3400 3410 3420 SNSFQSALVS LIRKMAIDHP YHTILQLLAL ANGDRIKDNQ RSRNSFVVDM DKKLAAEHLL 3430 3440 3450 3460 3470 3480 QDVSHYHGPM IRQMKQLVDI YIKLAELETR REDTNRKVAL PREIRSVKQL ELVPVVTATI 3490 3500 3510 3520 3530 3540 PVDRSCQYNE GSFPFFRGLS DSVTVMNGIN APKVVECFGS DGQKYKQLAK SGNDDLRQDA 3550 3560 3570 3580 3590 3600 VMEQFFGLVN TFLHNNRDTW KRRLAVRTYK VIPFTPSAGV LEWVDGTIPL GDYLIGSSRS 3610 3620 3630 3640 3650 3660 EGAHGRYGIG NWKYPKCREH MSSAKDKRKA FVDVCTNFRP VMHYFFLEKF LQPADWFVKR 3670 3680 3690 3700 3710 3720 LAYTRSVAAS SMVGYIVGLG DRHAMNILID QATAEVVHID LGVAFEQGLM LKTPERVPFR 3730 3740 3750 3760 3770 3780 LTRDIIDGMG ITGVEGVFRR CCEETLSVMR TNKEALLTIV EVFIHDPLYK WALSPLKALQ 3790 3800 3810 3820 3830 3840 RQKETEDYDG MNLEGLQEEF EGNKDATRAL MRVKQKLDGY EGGEMRSIHG QAQQLIQDAI 3850 DTDRLSHMFP GWGAWM

References

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[1]	"An ATM homologue from Arabidopsis thaliana: complete genomic organisation and expression analysis." Garcia V., Salanoubat M., Choisne N., Tissier A. Nucleic Acids Res. 28:1692-1699(2000) [PubMed: 10734187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"AtATM is essential for meiosis and the somatic response to DNA damage in plants." Garcia V., Bruchet H., Camescasse D., Granier F., Bouchez D., Tissier A. Plant Cell 15:119-132(2003) [PubMed: 12509526] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]	"ATM and ATR make distinct contributions to chromosome end protection and the maintenance of telomeric DNA in Arabidopsis." Vespa L., Couvillion M., Spangler E., Shippen D.E. Genes Dev. 19:2111-2115(2005) [PubMed: 16166376] [Abstract] Cited for: FUNCTION.
[5]	"Ionizing radiation-dependent gamma-H2AX focus formation requires ataxia telangiectasia mutated and ataxia telangiectasia mutated and Rad3-related." Friesner J.D., Liu B., Culligan K., Britt A.B. Mol. Biol. Cell 16:2566-2576(2005) [PubMed: 15772150] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ250248 mRNA. Translation: CAB86487.1. AL096856 Genomic DNA. Translation: CAB92122.1.
IPI	IPI00526448.
PIR	T51174.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9M3G7.
Genome annotation databases
GenomeReviews	Gene locus AT3G48190 in contig BA000014_GR.
Organism-specific databases
GeneFarm	4562.
TAIR	At3g48190.
Enzyme and pathway databases
BRENDA	2.7.11.1. 302.
Gene expression databases
ArrayExpress	Q9M3G7.
GermOnline	AT3G48190. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR015519. Ataxia_Telang_Mut. IPR003152. FATC. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR003151. PIK-rel_kinase_FAT. IPR014009. PIK_FAT. IPR000313. PWWP. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR11139:SF3. Ataxia_Telang_Mut. 1 hit.
Pfam	PF02259. FAT. 1 hit. PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. PF00855. PWWP. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS50812. PWWP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATM_ARATH

Accession

Primary (citable) accession number: Q9M3G7
Secondary accession number(s): Q9M4D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)