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Q9M3B6

- PKP4_ARATH

UniProt

Q9M3B6 - PKP4_ARATH

Protein

Plastidial pyruvate kinase 4, chloroplastic

Gene

PKP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651SubstrateBy similarity
    Metal bindingi267 – 2671PotassiumBy similarity
    Metal bindingi299 – 2991PotassiumBy similarity
    Sitei583 – 5831Transition state stabilizerBy similarity
    Metal bindingi585 – 5851MagnesiumSequence Analysis
    Binding sitei614 – 6141Substrate; via amide nitrogenBy similarity
    Metal bindingi615 – 6151MagnesiumBy similarity
    Binding sitei615 – 6151Substrate; via amide nitrogenBy similarity
    Binding sitei647 – 6471SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciARA:AT3G49160-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plastidial pyruvate kinase 4, chloroplastic (EC:2.7.1.40)
    Short name:
    PKp4
    Gene namesi
    Name:PKP4
    Ordered Locus Names:At3g49160
    ORF Names:F2K15.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G49160.

    Subcellular locationi

    Plastidchloroplast stroma By similarity

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 710Plastidial pyruvate kinase 4, chloroplasticPRO_0000416990
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PaxDbiQ9M3B6.
    PRIDEiQ9M3B6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9M3B6.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9M3B6. 7 interactions.
    STRINGi3702.AT3G49160.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M3B6.
    SMRiQ9M3B6. Positions 233-362, 437-694.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000224464.
    InParanoidiQ9M3B6.
    KOiK00873.
    OMAiPVIMATQ.
    PhylomeDBiQ9M3B6.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015793. Pyrv_Knase_brl.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 2 hits.
    [Graphical view]
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9M3B6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGLDSSHLL RDKILCFSSR SHINNQHKKT SYALSLNHMK LPIQRTLAFA    50
    LARGKGEAES FSRLEATFGD NTSTECTWSF DFPDSKDAMS HLKSEADLSG 100
    SNGANNVASV IEKLNALRSH LLAAEKWNAS QLHLCDSKYL ECATNLVHYM 150
    ALRSLDIEQL NSHLASLGLS SLDNNNLDVL AHLNASINLL MNDQNAVTES 200
    WTNVYPKGKS TKKNDKGRVL SYKESLLGKL REGRSTHIMV TIGEEATLSE 250
    TFITDILKAG TSVIRINCAH GDPSIWGEII KRVRRTSQML EMPCRVHMDL 300
    AGPKLRTGTL KPGPCVMKIS PKKDAYGNVV SPALVWLCLT GTEPPAHVSP 350
    DATISVQGQD FLAGLQIGDS IRLCDARGRK RRLKISKEFH VFNSTGFVAE 400
    CFDTAYIESG TELSVKGKKG RRLVGRVVDV PPKESFVRLK VGDLLVITRE 450
    GSLDEPSVTV PGAHRLTCPS GYLFDSVKPG ETIGFDDGKI WGVIKGTSPS 500
    EVIVSITHAR PKGTKLGSEK SINIPQSDIH FKGLTSKDIK DLDYVASHAD 550
    MVGISFIRDV HDITVLRQEL KKRKLDDLGI VLKIETKSGF KNLSLILLEA 600
    MKCSNPLGIM IARGDLAVEC GWERLANMQE EIIAICKAAR VPVIMATQVL 650
    ESLVKSGVPT RAEITDAANA KRASCVMLNK GKNIVEAVSM LDTILHTKLI 700
    YKKSDSENLH 710
    Length:710
    Mass (Da):77,827
    Last modified:October 1, 2000 - v1
    Checksum:i515D788AD6609E70
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL132956 Genomic DNA. Translation: CAB66395.1.
    CP002686 Genomic DNA. Translation: AEE78506.1.
    AY072177 mRNA. Translation: AAL59999.1.
    AY096527 mRNA. Translation: AAM20177.1.
    PIRiT45821.
    RefSeqiNP_190485.1. NM_114775.2.
    UniGeneiAt.35642.

    Genome annotation databases

    EnsemblPlantsiAT3G49160.1; AT3G49160.1; AT3G49160.
    GeneIDi824077.
    KEGGiath:AT3G49160.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL132956 Genomic DNA. Translation: CAB66395.1 .
    CP002686 Genomic DNA. Translation: AEE78506.1 .
    AY072177 mRNA. Translation: AAL59999.1 .
    AY096527 mRNA. Translation: AAM20177.1 .
    PIRi T45821.
    RefSeqi NP_190485.1. NM_114775.2.
    UniGenei At.35642.

    3D structure databases

    ProteinModelPortali Q9M3B6.
    SMRi Q9M3B6. Positions 233-362, 437-694.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9M3B6. 7 interactions.
    STRINGi 3702.AT3G49160.1-P.

    Proteomic databases

    PaxDbi Q9M3B6.
    PRIDEi Q9M3B6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G49160.1 ; AT3G49160.1 ; AT3G49160 .
    GeneIDi 824077.
    KEGGi ath:AT3G49160.

    Organism-specific databases

    TAIRi AT3G49160.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000224464.
    InParanoidi Q9M3B6.
    KOi K00873.
    OMAi PVIMATQ.
    PhylomeDBi Q9M3B6.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci ARA:AT3G49160-MONOMER.

    Gene expression databases

    Genevestigatori Q9M3B6.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015793. Pyrv_Knase_brl.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
      Andre C., Froehlich J.E., Moll M.R., Benning C.
      Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiPKP4_ARATH
    AccessioniPrimary (citable) accession number: Q9M3B6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3