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Q9M3B6

- PKP4_ARATH

UniProt

Q9M3B6 - PKP4_ARATH

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Protein

Plastidial pyruvate kinase 4, chloroplastic

Gene

PKP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651SubstrateBy similarity
Metal bindingi267 – 2671PotassiumBy similarity
Metal bindingi299 – 2991PotassiumBy similarity
Sitei583 – 5831Transition state stabilizerBy similarity
Metal bindingi585 – 5851MagnesiumSequence Analysis
Binding sitei614 – 6141Substrate; via amide nitrogenBy similarity
Metal bindingi615 – 6151MagnesiumBy similarity
Binding sitei615 – 6151Substrate; via amide nitrogenBy similarity
Binding sitei647 – 6471SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciARA:AT3G49160-MONOMER.
ReactomeiREACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 4, chloroplastic (EC:2.7.1.40)
Short name:
PKp4
Gene namesi
Name:PKP4
Ordered Locus Names:At3g49160
ORF Names:F2K15.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G49160.

Subcellular locationi

Plastidchloroplast stroma By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 710Plastidial pyruvate kinase 4, chloroplasticPRO_0000416990
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PaxDbiQ9M3B6.
PRIDEiQ9M3B6.

Expressioni

Gene expression databases

GenevestigatoriQ9M3B6.

Interactioni

Protein-protein interaction databases

IntActiQ9M3B6. 7 interactions.
STRINGi3702.AT3G49160.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M3B6.
SMRiQ9M3B6. Positions 233-362, 437-694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000224464.
InParanoidiQ9M3B6.
KOiK00873.
OMAiPVIMATQ.
PhylomeDBiQ9M3B6.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015793. Pyrv_Knase_brl.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 2 hits.
[Graphical view]
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M3B6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVGLDSSHLL RDKILCFSSR SHINNQHKKT SYALSLNHMK LPIQRTLAFA
60 70 80 90 100
LARGKGEAES FSRLEATFGD NTSTECTWSF DFPDSKDAMS HLKSEADLSG
110 120 130 140 150
SNGANNVASV IEKLNALRSH LLAAEKWNAS QLHLCDSKYL ECATNLVHYM
160 170 180 190 200
ALRSLDIEQL NSHLASLGLS SLDNNNLDVL AHLNASINLL MNDQNAVTES
210 220 230 240 250
WTNVYPKGKS TKKNDKGRVL SYKESLLGKL REGRSTHIMV TIGEEATLSE
260 270 280 290 300
TFITDILKAG TSVIRINCAH GDPSIWGEII KRVRRTSQML EMPCRVHMDL
310 320 330 340 350
AGPKLRTGTL KPGPCVMKIS PKKDAYGNVV SPALVWLCLT GTEPPAHVSP
360 370 380 390 400
DATISVQGQD FLAGLQIGDS IRLCDARGRK RRLKISKEFH VFNSTGFVAE
410 420 430 440 450
CFDTAYIESG TELSVKGKKG RRLVGRVVDV PPKESFVRLK VGDLLVITRE
460 470 480 490 500
GSLDEPSVTV PGAHRLTCPS GYLFDSVKPG ETIGFDDGKI WGVIKGTSPS
510 520 530 540 550
EVIVSITHAR PKGTKLGSEK SINIPQSDIH FKGLTSKDIK DLDYVASHAD
560 570 580 590 600
MVGISFIRDV HDITVLRQEL KKRKLDDLGI VLKIETKSGF KNLSLILLEA
610 620 630 640 650
MKCSNPLGIM IARGDLAVEC GWERLANMQE EIIAICKAAR VPVIMATQVL
660 670 680 690 700
ESLVKSGVPT RAEITDAANA KRASCVMLNK GKNIVEAVSM LDTILHTKLI
710
YKKSDSENLH
Length:710
Mass (Da):77,827
Last modified:October 1, 2000 - v1
Checksum:i515D788AD6609E70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132956 Genomic DNA. Translation: CAB66395.1.
CP002686 Genomic DNA. Translation: AEE78506.1.
AY072177 mRNA. Translation: AAL59999.1.
AY096527 mRNA. Translation: AAM20177.1.
PIRiT45821.
RefSeqiNP_190485.1. NM_114775.2.
UniGeneiAt.35642.

Genome annotation databases

EnsemblPlantsiAT3G49160.1; AT3G49160.1; AT3G49160.
GeneIDi824077.
KEGGiath:AT3G49160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132956 Genomic DNA. Translation: CAB66395.1 .
CP002686 Genomic DNA. Translation: AEE78506.1 .
AY072177 mRNA. Translation: AAL59999.1 .
AY096527 mRNA. Translation: AAM20177.1 .
PIRi T45821.
RefSeqi NP_190485.1. NM_114775.2.
UniGenei At.35642.

3D structure databases

ProteinModelPortali Q9M3B6.
SMRi Q9M3B6. Positions 233-362, 437-694.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9M3B6. 7 interactions.
STRINGi 3702.AT3G49160.1-P.

Proteomic databases

PaxDbi Q9M3B6.
PRIDEi Q9M3B6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G49160.1 ; AT3G49160.1 ; AT3G49160 .
GeneIDi 824077.
KEGGi ath:AT3G49160.

Organism-specific databases

TAIRi AT3G49160.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000224464.
InParanoidi Q9M3B6.
KOi K00873.
OMAi PVIMATQ.
PhylomeDBi Q9M3B6.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci ARA:AT3G49160-MONOMER.
Reactomei REACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.

Gene expression databases

Genevestigatori Q9M3B6.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015793. Pyrv_Knase_brl.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 2 hits.
[Graphical view ]
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
    Andre C., Froehlich J.E., Moll M.R., Benning C.
    Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiPKP4_ARATH
AccessioniPrimary (citable) accession number: Q9M3B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3