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Protein

Probable pectinesterase/pectinesterase inhibitor 34

Gene

PME34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Pectinesterase (PMEPCRF), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At3g43380), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (At5g26810), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (At3g49220), Probable pectinesterase 29 (PME29), Pectinesterase (AXX17_At4g38040), Pectinesterase (At5g20860), Probable pectinesterase 8 (PME8), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Probable pectinesterase 67 (PME67), Probable pectinesterase 56 (PME56), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Uncharacterized protein (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (PME44), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (At4g15980), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Uncharacterized protein (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Pectate lyase (At3g27400), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Pectate lyase (At5g04310), Probable pectate lyase 22 (At5g63180), Pectate lyase (At5g09280), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase, Pectate lyase, Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (At3g09540), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (AXX17_At4g28670), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase, Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei360Substrate; for pectinesterase activityBy similarity1
Binding sitei390Substrate; for pectinesterase activityBy similarity1
Sitei412Transition state stabilizerBy similarity1
Active sitei413Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei434Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei502Substrate; for pectinesterase activityBy similarity1
Binding sitei504Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall modification Source: InterPro
  • pectin catabolic process Source: UniProtKB-UniPathway
  • regulation of stomatal movement Source: TAIR

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 34
Including the following 2 domains:
Pectinesterase inhibitor 34
Alternative name(s):
Pectin methylesterase inhibitor 34
Pectinesterase 34 (EC:3.1.1.11)
Short name:
PE 34
Alternative name(s):
Pectin methylesterase 34
Short name:
AtPME34
Gene namesi
Name:PME34
Synonyms:ARATH34
Ordered Locus Names:At3g49220
ORF Names:F2K15.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G49220
TAIRilocus:2082951 AT3G49220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003716861 – 598Probable pectinesterase/pectinesterase inhibitor 34Add BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi427 ↔ 447By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9M3B0
PRIDEiQ9M3B0

PTM databases

iPTMnetiQ9M3B0

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

ExpressionAtlasiQ9M3B0 baseline and differential
GenevisibleiQ9M3B0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G49220.1

Structurei

3D structure databases

ProteinModelPortaliQ9M3B0
SMRiQ9M3B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 232Pectinesterase inhibitor 34Add BLAST152
Regioni284 – 582Pectinesterase 34Add BLAST299

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIF6 Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9M3B0
OMAiMMSYIGG
OrthoDBiEOG093605GG
PhylomeDBiQ9M3B0

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9M3B0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGYERLGPSG ATGSVTTSTT TAPILNQVST SEQPENNNRR SKKKLVVSSI
60 70 80 90 100
VLAISLILAA AIFAGVRSRL KLNQSVPGLA RKPSQAISKA CELTRFPELC
110 120 130 140 150
VDSLMDFPGS LAASSSKDLI HVTVNMTLHH FSHALYSSAS LSFVDMPPRA
160 170 180 190 200
RSAYDSCVEL LDDSVDALSR ALSSVVSSSA KPQDVTTWLS AALTNHDTCT
210 220 230 240 250
EGFDGVDDGG VKDHMTAALQ NLSELVSNCL AIFSASHDGD DFAGVPIQNR
260 270 280 290 300
RLLGVEEREE KFPRWMRPKE REILEMPVSQ IQADIIVSKD GNGTCKTISE
310 320 330 340 350
AIKKAPQNST RRIIIYVKAG RYEENNLKVG RKKINLMFVG DGKGKTVISG
360 370 380 390 400
GKSIFDNITT FHTASFAATG AGFIARDITF ENWAGPAKHQ AVALRIGADH
410 420 430 440 450
AVIYRCNIIG YQDTLYVHSN RQFFRECDIY GTVDFIFGNA AVVLQNCSIY
460 470 480 490 500
ARKPMDFQKN TITAQNRKDP NQNTGISIHA SRVLAASDLQ ATNGSTQTYL
510 520 530 540 550
GRPWKLFSRT VYMMSYIGGH VHTRGWLEWN TTFALDTLYY GEYLNSGPGS
560 570 580 590
GLGQRVSWPG YRVINSTAEA NRFTVAEFIY GSSWLPSTGV SFLAGLSI
Length:598
Mass (Da):65,307
Last modified:October 1, 2000 - v1
Checksum:i7FDB338A79857A6D
GO
Isoform 2 (identifier: Q9M3B0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-377: AATGAGFIARD → GEFFLTSLFLF
     378-598: Missing.

Show »
Length:377
Mass (Da):40,896
Checksum:iEE457950C82DF5F8
GO

Sequence cautioni

The sequence AAG40402 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037091367 – 377AATGAGFIARD → GEFFLTSLFLF in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_037092378 – 598Missing in isoform 2. 1 PublicationAdd BLAST221

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132956 Genomic DNA Translation: CAB66401.1
CP002686 Genomic DNA Translation: AEE78512.1
AY059834 mRNA Translation: AAL24316.1
BT008355 mRNA Translation: AAP37714.1
AF325050 mRNA Translation: AAG40402.1 Different initiation.
AK226562 mRNA Translation: BAE98692.1
AK226672 mRNA Translation: BAE98780.1
AK229067 mRNA Translation: BAF00948.1
PIRiT45827
RefSeqiNP_190491.1, NM_114781.5 [Q9M3B0-1]
UniGeneiAt.24776

Genome annotation databases

EnsemblPlantsiAT3G49220.1; AT3G49220.1; AT3G49220 [Q9M3B0-1]
AT3G49220.3; AT3G49220.3; AT3G49220
GeneIDi824083
GrameneiAT3G49220.1; AT3G49220.1; AT3G49220 [Q9M3B0-1]
AT3G49220.3; AT3G49220.3; AT3G49220
KEGGiath:AT3G49220

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPME34_ARATH
AccessioniPrimary (citable) accession number: Q9M3B0
Secondary accession number(s): Q0WPK1, Q0WVR8, Q9FPG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health