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Q9M384 (SCR_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SCARECROW

Short name=AtSCR
Alternative name(s):
GRAS family protein 20
Short name=AtGRAS-20
Protein SHOOT GRAVITROPISM 1
Gene names
Name:SCR
Synonyms:SGR1
Ordered Locus Names:At3g54220
ORF Names:F24B22.180
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for cell division but not differentiation of the ground tissue. Also required for normal shoot gravitropism. Regulates the radial organization of the shoot axial organs. Binds to the promoter of MGP, NUC, RLK and SCL3. Restricts SHR movment and sequesters it into the nucleus of the endodermis. Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14

Subunit structure

Interacts with SHR, JKD and MGP. Ref.13 Ref.14 Ref.15 Ref.16

Subcellular location

Nucleus.

Tissue specificity

Expressed in siliques, leaves and roots. Detected in the initial daughter cell before its asymmetric division and remains expressed only in the endodermal cell layer after the division. Expressed in the endodermis or starch sheath of the seedling hypocotyl, in the leaf bundle sheath cells and the root quiescent center. Ref.1 Ref.5 Ref.7 Ref.8 Ref.9

Developmental stage

Detected in the ground tissue of late heart-stage embryos. After germination, expressed also in the L1 layer throughout the shoot apical meristem including the peripheral zone. Detected in most tissues of young leaf primordia, except in the presumptive vasculature. In mature leaves, expressed in bundle sheath cells. Detected in inflorescence stems in a single internal cell layer corresponding to the starch sheath. Ref.1 Ref.7

Induction

Up-regulated by SHR and by itself. Ref.8 Ref.9 Ref.12

Disruption phenotype

Plants have a greatly reduced root length and only a single cell layer between the epidermis and the pericycle. Ref.1 Ref.6 Ref.7

Sequence similarities

Belongs to the GRAS family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 653653Protein SCARECROW
PRO_0000329415

Regions

Coiled coil262 – 28928 Potential
Motif401 – 4055VHIID
Compositional bias12 – 4029Pro-rich
Compositional bias22 – 287Poly-Ser
Compositional bias132 – 1387Poly-Ser
Compositional bias194 – 1974Poly-Ser
Compositional bias218 – 24023Gln-rich
Compositional bias232 – 2365Poly-Pro

Experimental info

Mutagenesis490 – 653164Missing in scr-3/sgr1-1; loss of shoot gravitropism. Ref.6
Sequence conflict4541A → T in AAB06318. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9M384 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 009A4C48C6DA0616

FASTA65371,506
        10         20         30         40         50         60 
MAESGDFNGG QPPPHSPLRT TSSGSSSSNN RGPPPPPPPP LVMVRKRLAS EMSSNPDYNN 

        70         80         90        100        110        120 
SSRPPRRVSH LLDSNYNTVT PQQPPSLTAA ATVSSQPNPP LSVCGFSGLP VFPSDRGGRN 

       130        140        150        160        170        180 
VMMSVQPMDQ DSSSSSASPT VWVDAIIRDL IHSSTSVSIP QLIQNVRDII FPCNPNLGAL 

       190        200        210        220        230        240 
LEYRLRSLML LDPSSSSDPS PQTFEPLYQI SNNPSPPQQQ QQHQQQQQQH KPPPPPIQQQ 

       250        260        270        280        290        300 
ERENSSTDAP PQPETVTATV PAVQTNTAEA LRERKEEIKR QKQDEEGLHL LTLLLQCAEA 

       310        320        330        340        350        360 
VSADNLEEAN KLLLEISQLS TPYGTSAQRV AAYFSEAMSA RLLNSCLGIY AALPSRWMPQ 

       370        380        390        400        410        420 
THSLKMVSAF QVFNGISPLV KFSHFTANQA IQEAFEKEDS VHIIDLDIMQ GLQWPGLFHI 

       430        440        450        460        470        480 
LASRPGGPPH VRLTGLGTSM EALQATGKRL SDFADKLGLP FEFCPLAEKV GNLDTERLNV 

       490        500        510        520        530        540 
RKREAVAVHW LQHSLYDVTG SDAHTLWLLQ RLAPKVVTVV EQDLSHAGSF LGRFVEAIHY 

       550        560        570        580        590        600 
YSALFDSLGA SYGEESEERH VVEQQLLSKE IRNVLAVGGP SRSGEVKFES WREKMQQCGF 

       610        620        630        640        650 
KGISLAGNAA TQATLLLGMF PSDGYTLVDD NGTLKLGWKD LSLLTASAWT PRS 

« Hide

References

« Hide 'large scale' references
[1]"The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root."
Di Laurenzio L., Wysocka-Diller J.W., Malamy J.E., Pysh L.D., Helariutta Y., Freshour G., Hahn M.G., Feldmann K.A., Benfey P.N.
Cell 86:423-433(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Analysis of SCARECROW expression using a rapid system for assessing transgene expression in Arabidopsis roots."
Malamy J.E., Benfey P.N.
Plant J. 12:957-963(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"Genetic evidence that the endodermis is essential for shoot gravitropism in Arabidopsis thaliana."
Fukaki H., Wysocka-Diller J.W., Kato T., Fujisawa H., Benfey P.N., Tasaka M.
Plant J. 14:425-430(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 490-TRP--SER-653.
[7]"Molecular analysis of SCARECROW function reveals a radial patterning mechanism common to root and shoot."
Wysocka-Diller J.W., Helariutta Y., Fukaki H., Malamy J.E., Benfey P.N.
Development 127:595-603(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[8]"The SHORT-ROOT gene controls radial patterning of the Arabidopsis root through radial signaling."
Helariutta Y., Fukaki H., Wysocka-Diller J.W., Nakajima K., Jung J., Sena G., Hauser M.-T., Benfey P.N.
Cell 101:555-567(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
[9]"Intercellular movement of the putative transcription factor SHR in root patterning."
Nakajima K., Sena G., Nawy T., Benfey P.N.
Nature 413:307-311(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
[10]"SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem."
Sabatini S., Heidstra R., Wildwater M., Scheres B.
Genes Dev. 17:354-358(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression."
Sena G., Jung J.W., Benfey P.N.
Development 131:2817-2826(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division."
Heidstra R., Welch D., Scheres B.
Genes Dev. 18:1964-1969(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[13]"Whole-genome analysis of the SHORT-ROOT developmental pathway in Arabidopsis."
Levesque M.P., Vernoux T., Busch W., Cui H., Wang J.Y., Blilou I., Hassan H., Nakajima K., Matsumoto N., Lohmann J.U., Scheres B., Benfey P.N.
PLoS Biol. 4:739-752(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SHR.
[14]"An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants."
Cui H., Levesque M.P., Vernoux T., Jung J.W., Paquette A.J., Gallagher K.L., Wang J.Y., Blilou I., Scheres B., Benfey P.N.
Science 316:421-425(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SHR.
[15]"Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action."
Welch D., Hassan H., Blilou I., Immink R., Heidstra R., Scheres B.
Genes Dev. 21:2196-2204(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHR; JKD AND MGP.
[16]"Large-scale analysis of the GRAS gene family in Arabidopsis thaliana."
Lee M.-H., Kim B., Song S.-K., Heo J.-O., Yu N.-I., Lee S.A., Kim M., Kim D.G., Sohn S.O., Lim C.E., Chang K.S., Lee M.M., Lim J.
Plant Mol. Biol. 67:659-670(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U62798 Genomic DNA. Translation: AAB06318.1.
AL132957 Genomic DNA. Translation: CAB70996.1.
CP002686 Genomic DNA. Translation: AEE79200.1.
AY056315 mRNA. Translation: AAL07164.1.
AY080840 mRNA. Translation: AAL87315.1.
AY113991 mRNA. Translation: AAM45039.1.
PIRT47581.
T51244.
RefSeqNP_190990.1. NM_115282.3.
UniGeneAt.71814.
At.932.

3D structure databases

ProteinModelPortalQ9M384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid9906. 4 interactions.
IntActQ9M384. 20 interactions.

Proteomic databases

PRIDEQ9M384.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G54220.1; AT3G54220.1; AT3G54220.
GeneID824589.
KEGGath:AT3G54220.

Organism-specific databases

GeneFarm4242. 427.
TAIRAT3G54220.

Phylogenomic databases

eggNOGNOG311437.
HOGENOMHOG000238577.
InParanoidQ9M384.
OMAFESWREK.
PhylomeDBQ9M384.
ProtClustDBCLSN2915643.

Gene expression databases

GenevestigatorQ9M384.

Family and domain databases

InterProIPR005202. TF_GRAS.
[Graphical view]
PfamPF03514. GRAS. 1 hit.
[Graphical view]
PROSITEPS50985. GRAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSCR_ARATH
AccessionPrimary (citable) accession number: Q9M384
Secondary accession number(s): Q96304
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names