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Protein

Protein SCARECROW

Gene

SCR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for cell division but not differentiation of the ground tissue. Also required for normal shoot gravitropism. Regulates the radial organization of the shoot axial organs. Binds to the promoter of MGP, NUC, RLK and SCL3. Restricts SHR movment and sequesters it into the nucleus of the endodermis.11 Publications

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • asymmetric cell division Source: TAIR
  • bundle sheath cell fate specification Source: TAIR
  • gravitropism Source: TAIR
  • leaf development Source: TAIR
  • maintenance of protein location in nucleus Source: TAIR
  • radial pattern formation Source: TAIR
  • regulation of transcription, DNA-templated Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SCARECROW
Short name:
AtSCR
Alternative name(s):
GRAS family protein 20
Short name:
AtGRAS-20
Protein SHOOT GRAVITROPISM 1
Gene namesi
Name:SCR
Synonyms:SGR1
Ordered Locus Names:At3g54220
ORF Names:F24B22.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54220.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants have a greatly reduced root length and only a single cell layer between the epidermis and the pericycle. The sgrl-1 mutant has no gravitropic response either in inflorescence stems or in hypocotyls.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi295 – 299LQCAE → AQCAA: Abolishes interaction with RBR1. 2 Publications5
Mutagenesisi490 – 653Missing in scr-3/sgr1-1; loss of shoot gravitropism. 1 PublicationAdd BLAST164

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003294151 – 653Protein SCARECROWAdd BLAST653

Proteomic databases

PaxDbiQ9M384.

Expressioni

Tissue specificityi

Expressed in siliques, leaves and roots. Detected in the initial daughter cell before its asymmetric division and remains expressed only in the endodermal cell layer after the division. Expressed in the endodermis or starch sheath of the seedling hypocotyl, in the leaf bundle sheath cells and the root quiescent center.5 Publications

Developmental stagei

Detected in the ground tissue of late heart-stage embryos. After germination, expressed also in the L1 layer throughout the shoot apical meristem including the peripheral zone. Detected in most tissues of young leaf primordia, except in the presumptive vasculature. In mature leaves, expressed in bundle sheath cells. Detected in inflorescence stems in a single internal cell layer corresponding to the starch sheath.2 Publications

Inductioni

Up-regulated by SHR and by itself.3 Publications

Gene expression databases

GenevisibleiQ9M384. AT.

Interactioni

Subunit structurei

Interacts with SHR, JKD and MGP (PubMed:16640459, PubMed:17446396, PubMed:17785527, PubMed:18500650). Interacts with SIEL (PubMed:21924907). Interacts with RBR1 through its the LxCxE motif (PubMed:22921914, PubMed:24302889).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MGPQ9ZWA63EBI-1250484,EBI-1568600
RBR1Q9LKZ34EBI-1250484,EBI-398590
SHRQ9SZF711EBI-1250484,EBI-1250472

Protein-protein interaction databases

BioGridi9906. 7 interactors.
IntActiQ9M384. 20 interactors.
STRINGi3702.AT3G54220.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M384.
SMRiQ9M384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili262 – 289Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi295 – 299LxCxE motif2 Publications5
Motifi401 – 405VHIID5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi12 – 40Pro-richAdd BLAST29
Compositional biasi22 – 28Poly-Ser7
Compositional biasi132 – 138Poly-Ser7
Compositional biasi194 – 197Poly-Ser4
Compositional biasi218 – 240Gln-richAdd BLAST23
Compositional biasi232 – 236Poly-Pro5

Sequence similaritiesi

Belongs to the GRAS family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IIRY. Eukaryota.
ENOG4110TCK. LUCA.
HOGENOMiHOG000238577.
InParanoidiQ9M384.
OMAiEVKFESW.
OrthoDBiEOG09360409.
PhylomeDBiQ9M384.

Family and domain databases

InterProiIPR005202. TF_GRAS.
[Graphical view]
PfamiPF03514. GRAS. 1 hit.
[Graphical view]
PROSITEiPS50985. GRAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESGDFNGG QPPPHSPLRT TSSGSSSSNN RGPPPPPPPP LVMVRKRLAS
60 70 80 90 100
EMSSNPDYNN SSRPPRRVSH LLDSNYNTVT PQQPPSLTAA ATVSSQPNPP
110 120 130 140 150
LSVCGFSGLP VFPSDRGGRN VMMSVQPMDQ DSSSSSASPT VWVDAIIRDL
160 170 180 190 200
IHSSTSVSIP QLIQNVRDII FPCNPNLGAL LEYRLRSLML LDPSSSSDPS
210 220 230 240 250
PQTFEPLYQI SNNPSPPQQQ QQHQQQQQQH KPPPPPIQQQ ERENSSTDAP
260 270 280 290 300
PQPETVTATV PAVQTNTAEA LRERKEEIKR QKQDEEGLHL LTLLLQCAEA
310 320 330 340 350
VSADNLEEAN KLLLEISQLS TPYGTSAQRV AAYFSEAMSA RLLNSCLGIY
360 370 380 390 400
AALPSRWMPQ THSLKMVSAF QVFNGISPLV KFSHFTANQA IQEAFEKEDS
410 420 430 440 450
VHIIDLDIMQ GLQWPGLFHI LASRPGGPPH VRLTGLGTSM EALQATGKRL
460 470 480 490 500
SDFADKLGLP FEFCPLAEKV GNLDTERLNV RKREAVAVHW LQHSLYDVTG
510 520 530 540 550
SDAHTLWLLQ RLAPKVVTVV EQDLSHAGSF LGRFVEAIHY YSALFDSLGA
560 570 580 590 600
SYGEESEERH VVEQQLLSKE IRNVLAVGGP SRSGEVKFES WREKMQQCGF
610 620 630 640 650
KGISLAGNAA TQATLLLGMF PSDGYTLVDD NGTLKLGWKD LSLLTASAWT

PRS
Length:653
Mass (Da):71,506
Last modified:October 1, 2000 - v1
Checksum:i009A4C48C6DA0616
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti454A → T in AAB06318 (PubMed:8756724).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62798 Genomic DNA. Translation: AAB06318.1.
AL132957 Genomic DNA. Translation: CAB70996.1.
CP002686 Genomic DNA. Translation: AEE79200.1.
AY056315 mRNA. Translation: AAL07164.1.
AY080840 mRNA. Translation: AAL87315.1.
AY113991 mRNA. Translation: AAM45039.1.
PIRiT47581.
T51244.
RefSeqiNP_190990.1. NM_115282.4.
UniGeneiAt.71814.
At.932.

Genome annotation databases

EnsemblPlantsiAT3G54220.1; AT3G54220.1; AT3G54220.
GeneIDi824589.
GrameneiAT3G54220.1; AT3G54220.1; AT3G54220.
KEGGiath:AT3G54220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62798 Genomic DNA. Translation: AAB06318.1.
AL132957 Genomic DNA. Translation: CAB70996.1.
CP002686 Genomic DNA. Translation: AEE79200.1.
AY056315 mRNA. Translation: AAL07164.1.
AY080840 mRNA. Translation: AAL87315.1.
AY113991 mRNA. Translation: AAM45039.1.
PIRiT47581.
T51244.
RefSeqiNP_190990.1. NM_115282.4.
UniGeneiAt.71814.
At.932.

3D structure databases

ProteinModelPortaliQ9M384.
SMRiQ9M384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9906. 7 interactors.
IntActiQ9M384. 20 interactors.
STRINGi3702.AT3G54220.1.

Proteomic databases

PaxDbiQ9M384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54220.1; AT3G54220.1; AT3G54220.
GeneIDi824589.
GrameneiAT3G54220.1; AT3G54220.1; AT3G54220.
KEGGiath:AT3G54220.

Organism-specific databases

TAIRiAT3G54220.

Phylogenomic databases

eggNOGiENOG410IIRY. Eukaryota.
ENOG4110TCK. LUCA.
HOGENOMiHOG000238577.
InParanoidiQ9M384.
OMAiEVKFESW.
OrthoDBiEOG09360409.
PhylomeDBiQ9M384.

Miscellaneous databases

PROiQ9M384.

Gene expression databases

GenevisibleiQ9M384. AT.

Family and domain databases

InterProiIPR005202. TF_GRAS.
[Graphical view]
PfamiPF03514. GRAS. 1 hit.
[Graphical view]
PROSITEiPS50985. GRAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCR_ARATH
AccessioniPrimary (citable) accession number: Q9M384
Secondary accession number(s): Q96304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.