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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial

Gene

KPHMT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (KPHMT1), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At2g43720), 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial (KPHMT2), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At3g55820)
  2. Putative 2-dehydropantoate 2-reductase (KPR)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85MagnesiumBy similarity1
Metal bindingi124MagnesiumBy similarity1
Binding sitei124Alpha-ketoisovalerateBy similarity1
Binding sitei154Alpha-ketoisovalerateBy similarity1
Metal bindingi156MagnesiumBy similarity1
Active sitei224Proton acceptorBy similarity1

GO - Molecular functioni

  • 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: TAIR
  • cobalt ion binding Source: TAIR
  • methyltransferase activity Source: UniProtKB-KW
  • zinc ion binding Source: TAIR

GO - Biological processi

  • pantothenate biosynthetic process Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial (EC:2.1.2.11)
Alternative name(s):
Ketopantoate hydroxymethyltransferase 2
Gene namesi
Name:KPHMT2
Synonyms:PANB2
Ordered Locus Names:At3g61530
ORF Names:F2A19.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G61530
TAIRilocus:2082847 AT3G61530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionSequence analysisAdd BLAST50
ChainiPRO_000042956751 – 3543-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrialAdd BLAST304

Proteomic databases

PaxDbiQ9M315
PRIDEiQ9M315

Expressioni

Gene expression databases

ExpressionAtlasiQ9M315 baseline and differential
GenevisibleiQ9M315 AT

Interactioni

Protein-protein interaction databases

BioGridi10640, 1 interactor
IntActiQ9M315, 1 interactor
STRINGi3702.AT3G61530.1

Structurei

3D structure databases

ProteinModelPortaliQ9M315
SMRiQ9M315
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Alpha-ketoisovalerate bindingBy similarity2

Sequence similaritiesi

Belongs to the PanB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2949 Eukaryota
COG0413 LUCA
HOGENOMiHOG000078427
InParanoidiQ9M315
KOiK00606
OMAiGHIGLMP
OrthoDBiEOG09360FPX
PhylomeDBiQ9M315

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASLVRSCC SRASRAITTV RFMSNVPEDT VYGGPKPQNS NQRVTLTQLR
60 70 80 90 100
QKHRKGEPIT MVTAYDYPSA VHIDTAGIDV CLVGDSAAMV VHGYDTTLPI
110 120 130 140 150
SLEEMLVHCR AVARGAKRPL LVGDLPFGTY ESSTNQAVDT AVRVLKEGGM
160 170 180 190 200
DAIKLEGGSP SRITAAKSIV EAGIAVMGHV GLTPQAISVL GGFRPQGKNI
210 220 230 240 250
ASAVKVVETA MALQEAGCFS VVLECVPPPV AAAATSALNI PTIGIGAGPF
260 270 280 290 300
CSGQVLVYHD LLGMMQHPHH AKVTPKFCKQ YAQVGEVINK ALLEYKEEVS
310 320 330 340 350
KHLFPGPSHS PYKISSSDLD GFLSELQKLG LDKAASDAAA SAEKMDHSDS

PSSQ
Length:354
Mass (Da):37,364
Last modified:October 1, 2000 - v1
Checksum:iE8CF63A1D0BCB4DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132962 Genomic DNA Translation: CAB71083.1
CP002686 Genomic DNA Translation: AEE80218.1
CP002686 Genomic DNA Translation: AEE80219.1
BT025255 mRNA Translation: ABF19008.1
AY087528 mRNA Translation: AAM65070.1
PIRiT47945
RefSeqiNP_191712.1, NM_116018.4
NP_974474.1, NM_202745.4
UniGeneiAt.20584

Genome annotation databases

EnsemblPlantsiAT3G61530.1; AT3G61530.1; AT3G61530
AT3G61530.2; AT3G61530.2; AT3G61530
GeneIDi825326
GrameneiAT3G61530.1; AT3G61530.1; AT3G61530
AT3G61530.2; AT3G61530.2; AT3G61530
KEGGiath:AT3G61530

Similar proteinsi

Entry informationi

Entry nameiPANB2_ARATH
AccessioniPrimary (citable) accession number: Q9M315
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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