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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial

Gene

KPHMT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (KPHMT1), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At2g43720), 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial (KPHMT2), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At3g55820)
  2. Putative 2-dehydropantoate 2-reductase (KPR)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85MagnesiumBy similarity1
Metal bindingi124MagnesiumBy similarity1
Binding sitei124Alpha-ketoisovalerateBy similarity1
Binding sitei154Alpha-ketoisovalerateBy similarity1
Metal bindingi156MagnesiumBy similarity1
Active sitei224Proton acceptorBy similarity1

GO - Molecular functioni

  • 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: TAIR
  • cobalt ion binding Source: TAIR
  • methyltransferase activity Source: UniProtKB-KW
  • zinc ion binding Source: TAIR

GO - Biological processi

  • pantothenate biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003.

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial (EC:2.1.2.11)
Alternative name(s):
Ketopantoate hydroxymethyltransferase 2
Gene namesi
Name:KPHMT2
Synonyms:PANB2
Ordered Locus Names:At3g61530
ORF Names:F2A19.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G61530.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionSequence analysisAdd BLAST50
ChainiPRO_000042956751 – 3543-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrialAdd BLAST304

Proteomic databases

PaxDbiQ9M315.

Expressioni

Gene expression databases

GenevisibleiQ9M315. AT.

Interactioni

Protein-protein interaction databases

BioGridi10640. 1 interactor.
IntActiQ9M315. 1 interactor.
STRINGi3702.AT3G61530.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M315.
SMRiQ9M315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Alpha-ketoisovalerate bindingBy similarity2

Sequence similaritiesi

Belongs to the PanB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2949. Eukaryota.
COG0413. LUCA.
HOGENOMiHOG000078427.
InParanoidiQ9M315.
KOiK00606.
OMAiRPQGKNI.
OrthoDBiEOG09360FPX.
PhylomeDBiQ9M315.

Family and domain databases

CDDicd06557. KPHMT-like. 1 hit.
Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB. 1 hit.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASLVRSCC SRASRAITTV RFMSNVPEDT VYGGPKPQNS NQRVTLTQLR
60 70 80 90 100
QKHRKGEPIT MVTAYDYPSA VHIDTAGIDV CLVGDSAAMV VHGYDTTLPI
110 120 130 140 150
SLEEMLVHCR AVARGAKRPL LVGDLPFGTY ESSTNQAVDT AVRVLKEGGM
160 170 180 190 200
DAIKLEGGSP SRITAAKSIV EAGIAVMGHV GLTPQAISVL GGFRPQGKNI
210 220 230 240 250
ASAVKVVETA MALQEAGCFS VVLECVPPPV AAAATSALNI PTIGIGAGPF
260 270 280 290 300
CSGQVLVYHD LLGMMQHPHH AKVTPKFCKQ YAQVGEVINK ALLEYKEEVS
310 320 330 340 350
KHLFPGPSHS PYKISSSDLD GFLSELQKLG LDKAASDAAA SAEKMDHSDS

PSSQ
Length:354
Mass (Da):37,364
Last modified:October 1, 2000 - v1
Checksum:iE8CF63A1D0BCB4DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132962 Genomic DNA. Translation: CAB71083.1.
CP002686 Genomic DNA. Translation: AEE80218.1.
CP002686 Genomic DNA. Translation: AEE80219.1.
BT025255 mRNA. Translation: ABF19008.1.
AY087528 mRNA. Translation: AAM65070.1.
PIRiT47945.
RefSeqiNP_191712.1. NM_116018.4.
NP_974474.1. NM_202745.4.
UniGeneiAt.20584.

Genome annotation databases

EnsemblPlantsiAT3G61530.1; AT3G61530.1; AT3G61530.
AT3G61530.2; AT3G61530.2; AT3G61530.
GeneIDi825326.
GrameneiAT3G61530.1; AT3G61530.1; AT3G61530.
AT3G61530.2; AT3G61530.2; AT3G61530.
KEGGiath:AT3G61530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132962 Genomic DNA. Translation: CAB71083.1.
CP002686 Genomic DNA. Translation: AEE80218.1.
CP002686 Genomic DNA. Translation: AEE80219.1.
BT025255 mRNA. Translation: ABF19008.1.
AY087528 mRNA. Translation: AAM65070.1.
PIRiT47945.
RefSeqiNP_191712.1. NM_116018.4.
NP_974474.1. NM_202745.4.
UniGeneiAt.20584.

3D structure databases

ProteinModelPortaliQ9M315.
SMRiQ9M315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10640. 1 interactor.
IntActiQ9M315. 1 interactor.
STRINGi3702.AT3G61530.1.

Proteomic databases

PaxDbiQ9M315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G61530.1; AT3G61530.1; AT3G61530.
AT3G61530.2; AT3G61530.2; AT3G61530.
GeneIDi825326.
GrameneiAT3G61530.1; AT3G61530.1; AT3G61530.
AT3G61530.2; AT3G61530.2; AT3G61530.
KEGGiath:AT3G61530.

Organism-specific databases

TAIRiAT3G61530.

Phylogenomic databases

eggNOGiKOG2949. Eukaryota.
COG0413. LUCA.
HOGENOMiHOG000078427.
InParanoidiQ9M315.
KOiK00606.
OMAiRPQGKNI.
OrthoDBiEOG09360FPX.
PhylomeDBiQ9M315.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003.

Miscellaneous databases

PROiQ9M315.

Gene expression databases

GenevisibleiQ9M315. AT.

Family and domain databases

CDDicd06557. KPHMT-like. 1 hit.
Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_00156. PanB. 1 hit.
InterProiIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiPF02548. Pantoate_transf. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPANB2_ARATH
AccessioniPrimary (citable) accession number: Q9M315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.