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Protein

Methionine--tRNA ligase, chloroplastic/mitochondrial

Gene

OVA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei369ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • methionine-tRNA ligase activity Source: TAIR

GO - Biological processi

  • methionyl-tRNA aminoacylation Source: GO_Central
  • plant ovule development Source: TAIR

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.10Curated)
Alternative name(s):
Methionyl-tRNA synthetaseCurated
Short name:
AtcpMetRS1 Publication
Short name:
MetRSCurated
Protein OVULE ABORTION 11 Publication
Gene namesi
Name:OVA11 Publication
Ordered Locus Names:At3g55400Imported
ORF Names:T22E16.60Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G55400
TAIRilocus:2099966 AT3G55400

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000433535? – 616Methionine--tRNA ligase, chloroplastic/mitochondrialCurated
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9M2T9
PRIDEiQ9M2T9

Expressioni

Gene expression databases

ExpressionAtlasiQ9M2T9 baseline and differential
GenevisibleiQ9M2T9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G55400.1

Structurei

3D structure databases

ProteinModelPortaliQ9M2T9
SMRiQ9M2T9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 88"HIGH" regionCuratedAdd BLAST11
Motifi366 – 370"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0436 Eukaryota
COG0143 LUCA
InParanoidiQ9M2T9
KOiK01874
OMAiTYVWFDA
OrthoDBiEOG093605RL
PhylomeDBiQ9M2T9

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_01228 Met_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014758 Met-tRNA_synth
IPR023457 Met-tRNA_synth_2
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF09334 tRNA-synt_1g, 2 hits
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 1 hit
TIGRFAMsiTIGR00398 metG, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According EST sequences.Curated
Isoform 1 (identifier: Q9M2T9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARINTSLH NALSFLKPFN TPLNTKPFSF RRNSFRFSKK LPYYSQFSSG
60 70 80 90 100
KRALYCTSSS QESTVDEGET FVLTTPLYYV NAPPHMGSAY TTIAADSIAR
110 120 130 140 150
FQRLLGKKVI FITGTDEHGE KIATSAAANG RNPPEHCDLI SQSYRTLWKD
160 170 180 190 200
LDIAYDKFIR TTDPKHEAIV KEFYARVFAN GDIYRADYEG LYCVNCEEYK
210 220 230 240 250
DEKELLENNC CPVHQMPCVA RKEDNYFFAL SKYQKPLEDI LAQNPRFVQP
260 270 280 290 300
SYRLNEVQSW IKSGLRDFSI SRALVDWGIP VPDDDKQTIY VWFDALLGYI
310 320 330 340 350
SALTEDNKQQ NLETAVSFGW PASLHLIGKD ILRFHAVYWP AMLMSAGLEL
360 370 380 390 400
PKMVFGHGFL TKDGMKMGKS LGNTLEPFEL VQKFGPDAVR YFFLREVEFG
410 420 430 440 450
NDGDYSEDRF IKIVNAHLAN TIGNLLNRTL GLLKKNCEST LVVDSTVAAE
460 470 480 490 500
GVPLKDTVEK LVEKARTNYE NLSLSSACEA VLEIGNAGNT YMDQRAPWFL
510 520 530 540 550
FKQGGVSAEE AAKDLVIILE VMRVIAVALS PVAPCLSLRI YSQLGYTEDQ
560 570 580 590 600
FNSITWSDTK WGGLKGGQVM EQASPVFARI ELNPEKEEDE KKPKVGKKTG
610
KAKVKVVEQT PTVAEA
Length:616
Mass (Da):69,274
Last modified:October 1, 2000 - v1
Checksum:i1E6A0B4DE127B4DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99A → S in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti204E → G in AAK32940 (PubMed:14593172).Curated1
Sequence conflicti204E → G in AAM91427 (PubMed:14593172).Curated1
Sequence conflicti344M → I in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti540I → V in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti547T → S in CAA74281 (PubMed:9724821).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13943 mRNA Translation: CAA74281.1
AL132975 Genomic DNA Translation: CAB75898.1
CP002686 Genomic DNA Translation: AEE79379.1
AF367354 mRNA Translation: AAK32940.1
AY133597 mRNA Translation: AAM91427.1
PIRiT47679
T50641
RefSeqiNP_191100.1, NM_115398.3 [Q9M2T9-1]
UniGeneiAt.249
At.35029

Genome annotation databases

EnsemblPlantsiAT3G55400.1; AT3G55400.1; AT3G55400 [Q9M2T9-1]
GeneIDi824706
GrameneiAT3G55400.1; AT3G55400.1; AT3G55400 [Q9M2T9-1]
KEGGiath:AT3G55400

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYMM_ARATH
AccessioniPrimary (citable) accession number: Q9M2T9
Secondary accession number(s): O23761, Q9ASP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health