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Protein

Methionine--tRNA ligase, chloroplastic/mitochondrial

Gene

OVA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei369ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • methionine-tRNA ligase activity Source: TAIR

GO - Biological processi

  • methionyl-tRNA aminoacylation Source: GO_Central
  • plant ovule development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G55400-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.10Curated)
Alternative name(s):
Methionyl-tRNA synthetaseCurated
Short name:
AtcpMetRS1 Publication
Short name:
MetRSCurated
Protein OVULE ABORTION 11 Publication
Gene namesi
Name:OVA11 Publication
Ordered Locus Names:At3g55400Imported
ORF Names:T22E16.60Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G55400.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: GO_Central
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000433535? – 616Methionine--tRNA ligase, chloroplastic/mitochondrialCurated
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9M2T9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9M2T9. baseline and differential.
GenevisibleiQ9M2T9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G55400.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M2T9.
SMRiQ9M2T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 88"HIGH" regionCuratedAdd BLAST11
Motifi366 – 370"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0436. Eukaryota.
COG0143. LUCA.
InParanoidiQ9M2T9.
KOiK01874.
OMAiPKEHCDA.
OrthoDBiEOG093605RL.
PhylomeDBiQ9M2T9.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 2 hits.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According EST sequences.Curated
Isoform 1 (identifier: Q9M2T9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARINTSLH NALSFLKPFN TPLNTKPFSF RRNSFRFSKK LPYYSQFSSG
60 70 80 90 100
KRALYCTSSS QESTVDEGET FVLTTPLYYV NAPPHMGSAY TTIAADSIAR
110 120 130 140 150
FQRLLGKKVI FITGTDEHGE KIATSAAANG RNPPEHCDLI SQSYRTLWKD
160 170 180 190 200
LDIAYDKFIR TTDPKHEAIV KEFYARVFAN GDIYRADYEG LYCVNCEEYK
210 220 230 240 250
DEKELLENNC CPVHQMPCVA RKEDNYFFAL SKYQKPLEDI LAQNPRFVQP
260 270 280 290 300
SYRLNEVQSW IKSGLRDFSI SRALVDWGIP VPDDDKQTIY VWFDALLGYI
310 320 330 340 350
SALTEDNKQQ NLETAVSFGW PASLHLIGKD ILRFHAVYWP AMLMSAGLEL
360 370 380 390 400
PKMVFGHGFL TKDGMKMGKS LGNTLEPFEL VQKFGPDAVR YFFLREVEFG
410 420 430 440 450
NDGDYSEDRF IKIVNAHLAN TIGNLLNRTL GLLKKNCEST LVVDSTVAAE
460 470 480 490 500
GVPLKDTVEK LVEKARTNYE NLSLSSACEA VLEIGNAGNT YMDQRAPWFL
510 520 530 540 550
FKQGGVSAEE AAKDLVIILE VMRVIAVALS PVAPCLSLRI YSQLGYTEDQ
560 570 580 590 600
FNSITWSDTK WGGLKGGQVM EQASPVFARI ELNPEKEEDE KKPKVGKKTG
610
KAKVKVVEQT PTVAEA
Length:616
Mass (Da):69,274
Last modified:October 1, 2000 - v1
Checksum:i1E6A0B4DE127B4DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99A → S in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti204E → G in AAK32940 (PubMed:14593172).Curated1
Sequence conflicti204E → G in AAM91427 (PubMed:14593172).Curated1
Sequence conflicti344M → I in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti540I → V in CAA74281 (PubMed:9724821).Curated1
Sequence conflicti547T → S in CAA74281 (PubMed:9724821).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13943 mRNA. Translation: CAA74281.1.
AL132975 Genomic DNA. Translation: CAB75898.1.
CP002686 Genomic DNA. Translation: AEE79379.1.
AF367354 mRNA. Translation: AAK32940.1.
AY133597 mRNA. Translation: AAM91427.1.
PIRiT47679.
T50641.
RefSeqiNP_191100.1. NM_115398.3. [Q9M2T9-1]
UniGeneiAt.249.
At.35029.

Genome annotation databases

EnsemblPlantsiAT3G55400.1; AT3G55400.1; AT3G55400. [Q9M2T9-1]
GeneIDi824706.
GrameneiAT3G55400.1; AT3G55400.1; AT3G55400.
KEGGiath:AT3G55400.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13943 mRNA. Translation: CAA74281.1.
AL132975 Genomic DNA. Translation: CAB75898.1.
CP002686 Genomic DNA. Translation: AEE79379.1.
AF367354 mRNA. Translation: AAK32940.1.
AY133597 mRNA. Translation: AAM91427.1.
PIRiT47679.
T50641.
RefSeqiNP_191100.1. NM_115398.3. [Q9M2T9-1]
UniGeneiAt.249.
At.35029.

3D structure databases

ProteinModelPortaliQ9M2T9.
SMRiQ9M2T9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G55400.1.

Proteomic databases

PaxDbiQ9M2T9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G55400.1; AT3G55400.1; AT3G55400. [Q9M2T9-1]
GeneIDi824706.
GrameneiAT3G55400.1; AT3G55400.1; AT3G55400.
KEGGiath:AT3G55400.

Organism-specific databases

TAIRiAT3G55400.

Phylogenomic databases

eggNOGiKOG0436. Eukaryota.
COG0143. LUCA.
InParanoidiQ9M2T9.
KOiK01874.
OMAiPKEHCDA.
OrthoDBiEOG093605RL.
PhylomeDBiQ9M2T9.

Enzyme and pathway databases

BioCyciARA:AT3G55400-MONOMER.

Miscellaneous databases

PROiQ9M2T9.

Gene expression databases

ExpressionAtlasiQ9M2T9. baseline and differential.
GenevisibleiQ9M2T9. AT.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 2 hits.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYMM_ARATH
AccessioniPrimary (citable) accession number: Q9M2T9
Secondary accession number(s): O23761, Q9ASP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.