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Protein

RNA cytidine acetyltransferase 2

Gene

At3g57940

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding adapter protein for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.UniRule annotation

Catalytic activityi

A cytidine in 18S rRNA + ATP + acetyl-CoA + H2O = a N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA.UniRule annotation
A cytidine in tRNA + ATP + acetyl-CoA + H2O = a N(4)-acetylcytidine in tRNAA + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei458ATPUniRule annotation1
Binding sitei717Acetyl-CoAUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 295ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA cytidine acetyltransferase 2UniRule annotation (EC:2.3.1.-UniRule annotation)
Alternative name(s):
18S rRNA cytosine acetyltransferase 2UniRule annotation
Gene namesi
Ordered Locus Names:At3g57940
ORF Names:T10K17.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G57940.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158881 – 1028RNA cytidine acetyltransferase 2Add BLAST1028

Proteomic databases

PaxDbiQ9M2Q4.

PTM databases

iPTMnetiQ9M2Q4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9M2Q4. baseline and differential.
GenevisibleiQ9M2Q4. AT.

Interactioni

Protein-protein interaction databases

BioGridi10278. 1 interactor.
MINTiMINT-8060991.
STRINGi3702.AT3G57940.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M2Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini546 – 729N-acetyltransferaseUniRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni617 – 619Acetyl-CoA bindingUniRule annotation3
Regioni624 – 630Acetyl-CoA bindingUniRule annotation7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi977 – 1020Ser-richAdd BLAST44

Sequence similaritiesi

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2036. Eukaryota.
COG1444. LUCA.
HOGENOMiHOG000210833.
InParanoidiQ9M2Q4.
KOiK14521.
OMAiSSHILNI.
OrthoDBiEOG0936023W.
PhylomeDBiQ9M2Q4.

Family and domain databases

HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PTHR10925:SF5. PTHR10925:SF5. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9M2Q4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKKVDERIR TLIENGVKLR HRSMFVIIGD KSRDQIVNLH HMLSKAVIKC
60 70 80 90 100
NPSVLWCYKD KLDISSHKQK RSKQLKRLRE RGQLDPEKLD AFSRLLDVGR
110 120 130 140 150
VTHCLYKDSE RILGNTFGMC ILQDFEALTP NLLARTIETV EGGGLVVLIL
160 170 180 190 200
RSLTSLTSLC TMVMDVHDRF RTESHSEAAG RFNERFLLSL ASCKACVVMD
210 220 230 240 250
DELNILPLSS HIRSITQVPT EKDSEGLSEA ERDLKSLKED LSDDFPVGPL
260 270 280 290 300
IKKCCTLDQG KAVVTFFDAI LDKALRSIVA LIASRGRGKS AALGLAVAGA
310 320 330 340 350
VAAGYSNIYI TAPSPDNLKT FFEFVCKGFD ALEYKEHLDY DVVKSANPDF
360 370 380 390 400
KKAVVRINIF KQHRQTIQYI QPHEHEKLSQ VELLVIDEAA AIPLPVVKSL
410 420 430 440 450
LGPYLVFLSS TVSGYEGTGR SLSLKLLQQL DEQSRAPATG LEGSGCLFKK
460 470 480 490 500
IELTESIRYG SGDPIESWLN GLLCLDVATC LPNPACHPSP SQCDLYYVNR
510 520 530 540 550
DTLFSYHKDS ELFLQRMMAL CVSSHYKNSP NDLQLLADAP AHHLFVLLGP
560 570 580 590 600
VDESQNKIPD ILCVIQVCLE GKISENSALQ SLRDGHSPYG DQIPWKFCEQ
610 620 630 640 650
FRDTEFPGFS GARIVRIAVH PNAMKMGYGS AAVELLTRYF EGQIAPISEA
660 670 680 690 700
EDKVDVEHAP IKVTEAAEKV SMLEEQVKPR TNLPPLLVPL HDRRPEKLHY
710 720 730 740 750
IGVSFGLTLD LFRFWRKHNF APFYVSQIPS AVTGEHTCML LKPLKNDELE
760 770 780 790 800
VNESDELGFF TPFYKDFKIR FSKLLSDKFK KMDYKLAMSV LNPKINFAEV
810 820 830 840 850
DSSGSSSGGF LKTLNGILSP YDMERLRAYT ENLTDFNLVY DICKTLAHQY
860 870 880 890 900
FEEKLPVSLS YVQASILLCL GLQETDFSSI ERQMQLERGQ IHSLLLKVAR
910 920 930 940 950
ELYKYLNGVA GKEIKSALPR LKERELTAHN VSVDDDIREG AKQVEEQMKK
960 970 980 990 1000
EKIEGLMDSE LQQYVIGDKE AEALQHSKIS SSGIISVKST KSENENGFDK
1010 1020
STKKRSSDKR SSSSSKSKSS KKRKSLKE
Length:1,028
Mass (Da):115,476
Last modified:June 26, 2007 - v2
Checksum:i5031CC843340284A
GO

Sequence cautioni

The sequence CAB67622 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132977 Genomic DNA. Translation: CAB67622.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79720.1.
AK229357 mRNA. Translation: BAF01220.1.
PIRiT46016.
RefSeqiNP_191353.2. NM_115656.4. [Q9M2Q4-1]
UniGeneiAt.34782.

Genome annotation databases

EnsemblPlantsiAT3G57940.1; AT3G57940.1; AT3G57940. [Q9M2Q4-1]
GeneIDi824963.
GrameneiAT3G57940.1; AT3G57940.1; AT3G57940.
KEGGiath:AT3G57940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132977 Genomic DNA. Translation: CAB67622.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79720.1.
AK229357 mRNA. Translation: BAF01220.1.
PIRiT46016.
RefSeqiNP_191353.2. NM_115656.4. [Q9M2Q4-1]
UniGeneiAt.34782.

3D structure databases

ProteinModelPortaliQ9M2Q4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10278. 1 interactor.
MINTiMINT-8060991.
STRINGi3702.AT3G57940.1.

PTM databases

iPTMnetiQ9M2Q4.

Proteomic databases

PaxDbiQ9M2Q4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G57940.1; AT3G57940.1; AT3G57940. [Q9M2Q4-1]
GeneIDi824963.
GrameneiAT3G57940.1; AT3G57940.1; AT3G57940.
KEGGiath:AT3G57940.

Organism-specific databases

TAIRiAT3G57940.

Phylogenomic databases

eggNOGiKOG2036. Eukaryota.
COG1444. LUCA.
HOGENOMiHOG000210833.
InParanoidiQ9M2Q4.
KOiK14521.
OMAiSSHILNI.
OrthoDBiEOG0936023W.
PhylomeDBiQ9M2Q4.

Miscellaneous databases

PROiQ9M2Q4.

Gene expression databases

ExpressionAtlasiQ9M2Q4. baseline and differential.
GenevisibleiQ9M2Q4. AT.

Family and domain databases

HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PTHR10925:SF5. PTHR10925:SF5. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNT102_ARATH
AccessioniPrimary (citable) accession number: Q9M2Q4
Secondary accession number(s): Q0WNS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 26, 2007
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.