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Protein

Agamous-like MADS-box protein AGL18

Gene

AGL18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable transcription factor involved in the negative regulation of flowering, probably through the photoperiodic pathway. Prevents premature flowering. Downstream regulator of a subset of the MIKC* MADS-controlled genes required during pollen maturation.3 Publications

GO - Molecular functioni

GO - Biological processi

  • flower development Source: UniProtKB-KW
  • MAPK cascade Source: InterPro
  • negative regulation of flower development Source: UniProtKB
  • negative regulation of short-day photoperiodism, flowering Source: UniProtKB
  • pollen development Source: TAIR
  • positive regulation of transcription from RNA polymerase II promoter Source: InterPro
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Agamous-like MADS-box protein AGL18
Gene namesi
Name:AGL18
Ordered Locus Names:At3g57390
ORF Names:F28O9.240
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G57390.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Altered expression of MIKC* MADS-controlled genes during pollen maturation. Early flowering under short-days conditions (SD) when combined with AGL15 disruption.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004125311 – 256Agamous-like MADS-box protein AGL18Add BLAST256

Proteomic databases

PaxDbiQ9M2K8.

Expressioni

Tissue specificityi

Mostly expressed in pollen, roots, flowers and siliques, and to a lower extent, in stems and leaves. Expressed in the endosperm and in developing male and female gametophytes. Also present in seedlings.5 Publications

Developmental stagei

During the reproductive phase, accumulates in immature buds and at the base of the floral organs, and in the receptacle, ovules, anther filaments, and stigma and style of open flowers. Later observed in sporogenous tissue of anthers. During male gametogenesis, expressed in the microspores before they separate from each other. Later present at high levels within pollen grains up to stage 13 of flower development, when anthers dehisce. During carpel development, first detected in developing ovules. After fertilization, confined to globular structures or nodules of proliferating free nuclear endosperm required for embryo development. Disappears from the endosperm at to the heart stage of embryo development, when very little nuclear endosperm remains. Never detected in developing embryos at any stage. In young seedlings, present everywhere except in a portion of the hypocotyl and in newly emerging leaves.2 Publications

Gene expression databases

GenevisibleiQ9M2K8. AT.

Interactioni

Protein-protein interaction databases

BioGridi10222. 3 interactors.
STRINGi3702.AT3G57390.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M2K8.
SMRiQ9M2K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 61MADS-boxPROSITE-ProRule annotationAdd BLAST61
Domaini94 – 184K-boxPROSITE-ProRule annotationAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi205 – 208Poly-Ser4

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiQ9M2K8.
OMAiKDQKTQI.
OrthoDBiEOG09360OGB.
PhylomeDBiQ9M2K8.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9M2K8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRIEIKK IENINSRQVT FSKRRNGLIK KAKELSILCD AEVALIIFSS
60 70 80 90 100
TGKIYDFSSV CMEQILSRYG YTTASTEHKQ QREHQLLICA SHGNEAVLRN
110 120 130 140 150
DDSMKGELER LQLAIERLKG KELEGMSFPD LISLENQLNE SLHSVKDQKT
160 170 180 190 200
QILLNQIERS RIQEKKALEE NQILRKQVEM LGRGSGPKVL NERPQDSSPE
210 220 230 240 250
ADPESSSSEE DENDNEEHHS DTSLQLGLSS TGYCTKRKKP KIELVCDNSG

SQVASD
Length:256
Mass (Da):28,874
Last modified:October 1, 2000 - v1
Checksum:iECF106A22F6F8627
GO
Isoform 2 (identifier: Q9M2K8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.
Show »
Length:195
Mass (Da):22,015
Checksum:i860631E803034575
GO
Isoform 3 (identifier: Q9M2K8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-154: TQILL → VKTKY
     155-256: Missing.

Note: No experimental confirmation available.
Show »
Length:154
Mass (Da):17,588
Checksum:i48D574DD7FE3C7FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106G → V in AAM65178 (Ref. 6) Curated1
Sequence conflicti134L → F in AAM65178 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0416891 – 61Missing in isoform 2. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_041690150 – 154TQILL → VKTKY in isoform 3. 1 Publication5
Alternative sequenceiVSP_041691155 – 256Missing in isoform 3. 1 PublicationAdd BLAST102

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312663 mRNA. Translation: AAG37900.1.
AL137080 Genomic DNA. Translation: CAB68145.1.
CP002686 Genomic DNA. Translation: AEE79649.1.
CP002686 Genomic DNA. Translation: AEE79650.1.
AK220679 mRNA. Translation: BAD93735.1.
AK222220 mRNA. Translation: BAD95389.1.
BT025774 mRNA. Translation: ABF83664.1.
AY087639 mRNA. Translation: AAM65178.1.
PIRiT45817.
RefSeqiNP_191298.1. NM_115599.4. [Q9M2K8-1]
NP_974450.1. NM_202721.1. [Q9M2K8-2]
UniGeneiAt.24026.

Genome annotation databases

EnsemblPlantsiAT3G57390.1; AT3G57390.1; AT3G57390. [Q9M2K8-1]
GeneIDi824906.
GrameneiAT3G57390.1; AT3G57390.1; AT3G57390.
KEGGiath:AT3G57390.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312663 mRNA. Translation: AAG37900.1.
AL137080 Genomic DNA. Translation: CAB68145.1.
CP002686 Genomic DNA. Translation: AEE79649.1.
CP002686 Genomic DNA. Translation: AEE79650.1.
AK220679 mRNA. Translation: BAD93735.1.
AK222220 mRNA. Translation: BAD95389.1.
BT025774 mRNA. Translation: ABF83664.1.
AY087639 mRNA. Translation: AAM65178.1.
PIRiT45817.
RefSeqiNP_191298.1. NM_115599.4. [Q9M2K8-1]
NP_974450.1. NM_202721.1. [Q9M2K8-2]
UniGeneiAt.24026.

3D structure databases

ProteinModelPortaliQ9M2K8.
SMRiQ9M2K8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10222. 3 interactors.
STRINGi3702.AT3G57390.1.

Proteomic databases

PaxDbiQ9M2K8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G57390.1; AT3G57390.1; AT3G57390. [Q9M2K8-1]
GeneIDi824906.
GrameneiAT3G57390.1; AT3G57390.1; AT3G57390.
KEGGiath:AT3G57390.

Organism-specific databases

TAIRiAT3G57390.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiQ9M2K8.
OMAiKDQKTQI.
OrthoDBiEOG09360OGB.
PhylomeDBiQ9M2K8.

Miscellaneous databases

PROiQ9M2K8.

Gene expression databases

GenevisibleiQ9M2K8. AT.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGL18_ARATH
AccessioniPrimary (citable) accession number: Q9M2K8
Secondary accession number(s): Q1ECG0, Q570M9, Q8LAS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.