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Protein

ATPase 8, plasma membrane-type

Gene

AHA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses (By similarity).By similarity

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 33214-aspartylphosphate intermediateBy similarity
Metal bindingi591 – 5911MagnesiumBy similarity
Metal bindingi595 – 5951MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G42640-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 8, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 8
Gene namesi
Name:AHA8
Ordered Locus Names:At3g42640
ORF Names:T12K4_90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G42640.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6464CytoplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8420Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini85 – 9612ExtracellularSequence analysisAdd
BLAST
Transmembranei97 – 11721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini118 – 246129CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26721Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini268 – 2769ExtracellularSequence analysis
Transmembranei277 – 29418Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini295 – 646352CytoplasmicSequence analysisAdd
BLAST
Transmembranei647 – 66822Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini669 – 6735ExtracellularSequence analysis
Transmembranei674 – 69623Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini697 – 71216CytoplasmicSequence analysisAdd
BLAST
Transmembranei713 – 73321Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini734 – 75421ExtracellularSequence analysisAdd
BLAST
Transmembranei755 – 77521Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini776 – 78712CytoplasmicSequence analysisAdd
BLAST
Transmembranei788 – 80821Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini809 – 8168ExtracellularSequence analysis
Transmembranei817 – 83721Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini838 – 948111CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948ATPase 8, plasma membrane-typePRO_0000046281Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei884 – 8841PhosphothreonineBy similarity
Modified residuei930 – 9301PhosphoserineBy similarity
Modified residuei947 – 9471PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9M2A0.
PRIDEiQ9M2A0.

PTM databases

iPTMnetiQ9M2A0.

Expressioni

Gene expression databases

GenevisibleiQ9M2A0. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-947. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

BioGridi8606. 20 interactions.
STRINGi3702.AT3G42640.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M2A0.
SMRiQ9M2A0. Positions 15-846, 919-948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni946 – 9483Interaction with 14-3-3 proteinsBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9M2A0.
KOiK01535.
OMAiHEVIDGF.
OrthoDBiEOG093602AD.
PhylomeDBiQ9M2A0.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M2A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATEFSWDEI KKENVDLERI PVEEVFEQLK CSKEGLSSDE GAKRLEIFGA
60 70 80 90 100
NKLEEKSENK FLKFLGFMWN PLSWVMESAA IMAIVLANGG GKAPDWQDFI
110 120 130 140 150
GIMVLLIINS TISFIEENNA GNAAAALMAN LAPKTKVLRD GKWGEQEASI
160 170 180 190 200
LVPGDLISIK LGDIVPADAR LLEGDPLKID QSALTGESLP TTKHPGDEVF
210 220 230 240 250
SGSTCKQGEI EAVVIATGVH TFFGKAAHLV DSTNNVGHFQ KVLTSIGNFC
260 270 280 290 300
ICSIGLGMLI EILIMYPIQH RTYRDGIDNL LVLLIGGIPI AMPTVLSVTM
310 320 330 340 350
AIGSHRLSQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDKSLIE
360 370 380 390 400
VFPKNMDSDS VVLMAARASR IENQDAIDAS IVGMLGDPKE ARAGITEVHF
410 420 430 440 450
LPFNPVDKRT AITYIDESGD WHRSSKGAPE QIIELCNLQG ETKRKAHEVI
460 470 480 490 500
DGFAERGLRS LGVAQQTVPE KTKESDGSPW EFVGLLPLFD PPRHDSAETI
510 520 530 540 550
RRALELGVNV KMITGDQLAI GIETGRRLGM GTNMYPSTSL LGNSKDESLV
560 570 580 590 600
GIPIDELIEK ADGFAGVFPE HKYEIVKKLQ ERKHICGMTG DGVNDAPALK
610 620 630 640 650
KADIGIAVAD ATDAARSASD IVLTEPGLSV IISAVLTSRA IFQRMKNYTI
660 670 680 690 700
YAVSITIRIV LGFMLVALIW RFDFAPFMVL IIAILNDGTI MTISKDRVKP
710 720 730 740 750
SPVPDSWKLN EIFATGVVLG TYMALTTVLF FWLAHDTDFF SKTFGVRSIQ
760 770 780 790 800
GNEEELMAAL YLQVSIISQA LIFVTRSRSW SFVERPGFLL LIAFVIAQLV
810 820 830 840 850
ATLIAVYANW GFARIVGCGW GWAGGIWVYS IITYIPLDIL KFIIRYALTG
860 870 880 890 900
KAWDNMINQK TAFTTKKDYG KGEREAQWAL AQRTLHGLPP PEAMFNDNKN
910 920 930 940
ELSEIAEQAK RRAEVARLRE LHTLKGHVES VVKLKGLDID TIQQHYTV
Length:948
Mass (Da):104,131
Last modified:October 1, 2000 - v1
Checksum:iA45CA0F478F7576B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138640 Genomic DNA. Translation: CAB86447.1.
CP002686 Genomic DNA. Translation: AEE77743.1.
PIRiT47322.
RefSeqiNP_189850.1. NM_114131.2.
UniGeneiAt.43630.

Genome annotation databases

EnsemblPlantsiAT3G42640.1; AT3G42640.1; AT3G42640.
GeneIDi823281.
GrameneiAT3G42640.1; AT3G42640.1; AT3G42640.
KEGGiath:AT3G42640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138640 Genomic DNA. Translation: CAB86447.1.
CP002686 Genomic DNA. Translation: AEE77743.1.
PIRiT47322.
RefSeqiNP_189850.1. NM_114131.2.
UniGeneiAt.43630.

3D structure databases

ProteinModelPortaliQ9M2A0.
SMRiQ9M2A0. Positions 15-846, 919-948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi8606. 20 interactions.
STRINGi3702.AT3G42640.1.

PTM databases

iPTMnetiQ9M2A0.

Proteomic databases

PaxDbiQ9M2A0.
PRIDEiQ9M2A0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G42640.1; AT3G42640.1; AT3G42640.
GeneIDi823281.
GrameneiAT3G42640.1; AT3G42640.1; AT3G42640.
KEGGiath:AT3G42640.

Organism-specific databases

TAIRiAT3G42640.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9M2A0.
KOiK01535.
OMAiHEVIDGF.
OrthoDBiEOG093602AD.
PhylomeDBiQ9M2A0.

Enzyme and pathway databases

BioCyciARA:AT3G42640-MONOMER.

Miscellaneous databases

PROiQ9M2A0.

Gene expression databases

GenevisibleiQ9M2A0. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA8_ARATH
AccessioniPrimary (citable) accession number: Q9M2A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.