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Q9M1Z5 (MPK10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 10

Short name=AtMPK10
Short name=MAP kinase 10
EC=2.7.11.24
Gene names
Name:MPK10
Ordered Locus Names:At3g59790
ORF Names:F24G16.60
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Subunit structure

Interacts with MKK2. Ref.5

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Mitogen-activated protein kinase 10
PRO_0000245810

Regions

Domain60 – 345286Protein kinase
Nucleotide binding66 – 749ATP By similarity
Motif218 – 2203TXY

Sites

Active site1861Proton acceptor By similarity
Binding site891ATP By similarity

Amino acid modifications

Modified residue2181Phosphothreonine By similarity
Modified residue2201Phosphotyrosine By similarity
Modified residue2231Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9M1Z5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F3BB2395C43CB38E

FASTA39345,174
        10         20         30         40         50         60 
MEPTNDAETL ETQGEVTTAI WPSSQILKTT IDIPGTLSHD GRYIQYNLFG HIFELPAKYK 

        70         80         90        100        110        120 
PPIRPIGRGA CGIVCSAVDS ETNEKVAIKK ITQVFDNTIE AKRTLREIKL LRHFDHENIV 

       130        140        150        160        170        180 
AIRDVILPPQ RDSFEDVYIV NELMEFDLYR TLKSDQELTK DHGMYFMYQI LRGLKYIHSA 

       190        200        210        220        230        240 
NVLHRDLKPS NLLLSTQCDL KICDFGLARA TPESNLMTEY VVTRWYRAPE LLLGSSDYTA 

       250        260        270        280        290        300 
AIDVWSVGCI FMEIMNREPL FPGKDQVNQL RLLLELIGTP SEEELGSLSE YAKRYIRQLP 

       310        320        330        340        350        360 
TLPRQSFTEK FPNVPPLAID LVEKMLTFDP KQRISVKEAL AHPYLSSFHD ITDEPECSEP 

       370        380        390 
FNFDLDEHPF SEEQFRELIY CEALAFNPET SND 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[4]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[5]"Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL138647 Genomic DNA. Translation: CAB75798.1.
CP002686 Genomic DNA. Translation: AEE79969.1.
PIRT47803.
RefSeqNP_191538.1. NM_115841.1.
UniGeneAt.54009.

3D structure databases

ProteinModelPortalQ9M1Z5.
SMRQ9M1Z5. Positions 35-388.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9M1Z5. 2 interactions.
STRING3702.AT3G59790.1-P.

Proteomic databases

PaxDbQ9M1Z5.
PRIDEQ9M1Z5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G59790.1; AT3G59790.1; AT3G59790.
GeneID825148.
KEGGath:AT3G59790.

Organism-specific databases

GeneFarm844. 89.
TAIRAT3G59790.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9M1Z5.
KOK04371.
OMACEALAFN.
PhylomeDBQ9M1Z5.

Enzyme and pathway databases

BioCycARA:AT3G59790-MONOMER.

Gene expression databases

GenevestigatorQ9M1Z5.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK10_ARATH
AccessionPrimary (citable) accession number: Q9M1Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names