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Protein

Mitogen-activated protein kinase 10

Gene

MPK10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89ATPPROSITE-ProRule annotation1
Active sitei186Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 74ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • auxin transport Source: TAIR
  • regulation of gene expression Source: GO_Central
  • xylem and phloem pattern formation Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056 MAPK3 (ERK1) activation
R-ATH-112409 RAF-independent MAPK1/3 activation
R-ATH-112411 MAPK1 (ERK2) activation
R-ATH-198753 ERK/MAPK targets
R-ATH-198765 Signalling to ERK5
R-ATH-202670 ERKs are inactivated
R-ATH-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-375165 NCAM signaling for neurite out-growth
R-ATH-442742 CREB phosphorylation through the activation of Ras
R-ATH-444257 RSK activation
R-ATH-445144 Signal transduction by L1
R-ATH-5673001 RAF/MAP kinase cascade
R-ATH-5674135 MAP2K and MAPK activation
R-ATH-5674499 Negative feedback regulation of MAPK pathway
R-ATH-6798695 Neutrophil degranulation
R-ATH-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 10 (EC:2.7.11.24)
Short name:
AtMPK10
Short name:
MAP kinase 10
Gene namesi
Name:MPK10
Ordered Locus Names:At3g59790
ORF Names:F24G16.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G59790
TAIRilocus:2080457 AT3G59790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458101 – 393Mitogen-activated protein kinase 10Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphothreonineBy similarity1
Modified residuei220PhosphotyrosineBy similarity1
Modified residuei223PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9M1Z5
PRIDEiQ9M1Z5

PTM databases

iPTMnetiQ9M1Z5

Expressioni

Gene expression databases

ExpressionAtlasiQ9M1Z5 baseline and differential
GenevisibleiQ9M1Z5 AT

Interactioni

Subunit structurei

Interacts with MKK2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi10462, 4 interactors
IntActiQ9M1Z5, 2 interactors
STRINGi3702.AT3G59790.1

Structurei

3D structure databases

ProteinModelPortaliQ9M1Z5
SMRiQ9M1Z5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 345Protein kinasePROSITE-ProRule annotationAdd BLAST286

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi218 – 220TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ9M1Z5
KOiK04371
OMAiCEALAFN
OrthoDBiEOG093613GQ
PhylomeDBiQ9M1Z5

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M1Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTNDAETL ETQGEVTTAI WPSSQILKTT IDIPGTLSHD GRYIQYNLFG
60 70 80 90 100
HIFELPAKYK PPIRPIGRGA CGIVCSAVDS ETNEKVAIKK ITQVFDNTIE
110 120 130 140 150
AKRTLREIKL LRHFDHENIV AIRDVILPPQ RDSFEDVYIV NELMEFDLYR
160 170 180 190 200
TLKSDQELTK DHGMYFMYQI LRGLKYIHSA NVLHRDLKPS NLLLSTQCDL
210 220 230 240 250
KICDFGLARA TPESNLMTEY VVTRWYRAPE LLLGSSDYTA AIDVWSVGCI
260 270 280 290 300
FMEIMNREPL FPGKDQVNQL RLLLELIGTP SEEELGSLSE YAKRYIRQLP
310 320 330 340 350
TLPRQSFTEK FPNVPPLAID LVEKMLTFDP KQRISVKEAL AHPYLSSFHD
360 370 380 390
ITDEPECSEP FNFDLDEHPF SEEQFRELIY CEALAFNPET SND
Length:393
Mass (Da):45,174
Last modified:October 1, 2000 - v1
Checksum:iF3BB2395C43CB38E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138647 Genomic DNA Translation: CAB75798.1
CP002686 Genomic DNA Translation: AEE79969.1
PIRiT47803
RefSeqiNP_191538.1, NM_115841.2

Genome annotation databases

EnsemblPlantsiAT3G59790.1; AT3G59790.1; AT3G59790
GeneIDi825148
GrameneiAT3G59790.1; AT3G59790.1; AT3G59790
KEGGiath:AT3G59790

Similar proteinsi

Entry informationi

Entry nameiMPK10_ARATH
AccessioniPrimary (citable) accession number: Q9M1Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health