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Q9M1Z5

- MPK10_ARATH

UniProt

Q9M1Z5 - MPK10_ARATH

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Protein

Mitogen-activated protein kinase 10

Gene

MPK10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891ATPPROSITE-ProRule annotation
Active sitei186 – 1861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 749ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G59790-MONOMER.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 10 (EC:2.7.11.24)
Short name:
AtMPK10
Short name:
MAP kinase 10
Gene namesi
Name:MPK10
Ordered Locus Names:At3g59790
ORF Names:F24G16.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G59790.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Mitogen-activated protein kinase 10PRO_0000245810Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181PhosphothreonineBy similarity
Modified residuei220 – 2201PhosphotyrosineBy similarity
Modified residuei223 – 2231PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9M1Z5.
PRIDEiQ9M1Z5.

Expressioni

Gene expression databases

GenevestigatoriQ9M1Z5.

Interactioni

Subunit structurei

Interacts with MKK2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK2Q9S7U92EBI-2358527,EBI-994350
MKK9Q9FX432EBI-2358527,EBI-2128545

Protein-protein interaction databases

IntActiQ9M1Z5. 2 interactions.
STRINGi3702.AT3G59790.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M1Z5.
SMRiQ9M1Z5. Positions 49-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 345286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi218 – 2203TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9M1Z5.
KOiK04371.
OMAiCEALAFN.
PhylomeDBiQ9M1Z5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M1Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPTNDAETL ETQGEVTTAI WPSSQILKTT IDIPGTLSHD GRYIQYNLFG
60 70 80 90 100
HIFELPAKYK PPIRPIGRGA CGIVCSAVDS ETNEKVAIKK ITQVFDNTIE
110 120 130 140 150
AKRTLREIKL LRHFDHENIV AIRDVILPPQ RDSFEDVYIV NELMEFDLYR
160 170 180 190 200
TLKSDQELTK DHGMYFMYQI LRGLKYIHSA NVLHRDLKPS NLLLSTQCDL
210 220 230 240 250
KICDFGLARA TPESNLMTEY VVTRWYRAPE LLLGSSDYTA AIDVWSVGCI
260 270 280 290 300
FMEIMNREPL FPGKDQVNQL RLLLELIGTP SEEELGSLSE YAKRYIRQLP
310 320 330 340 350
TLPRQSFTEK FPNVPPLAID LVEKMLTFDP KQRISVKEAL AHPYLSSFHD
360 370 380 390
ITDEPECSEP FNFDLDEHPF SEEQFRELIY CEALAFNPET SND
Length:393
Mass (Da):45,174
Last modified:October 1, 2000 - v1
Checksum:iF3BB2395C43CB38E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138647 Genomic DNA. Translation: CAB75798.1.
CP002686 Genomic DNA. Translation: AEE79969.1.
PIRiT47803.
RefSeqiNP_191538.1. NM_115841.1.
UniGeneiAt.54009.

Genome annotation databases

EnsemblPlantsiAT3G59790.1; AT3G59790.1; AT3G59790.
GeneIDi825148.
KEGGiath:AT3G59790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138647 Genomic DNA. Translation: CAB75798.1 .
CP002686 Genomic DNA. Translation: AEE79969.1 .
PIRi T47803.
RefSeqi NP_191538.1. NM_115841.1.
UniGenei At.54009.

3D structure databases

ProteinModelPortali Q9M1Z5.
SMRi Q9M1Z5. Positions 49-388.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9M1Z5. 2 interactions.
STRINGi 3702.AT3G59790.1-P.

Proteomic databases

PaxDbi Q9M1Z5.
PRIDEi Q9M1Z5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G59790.1 ; AT3G59790.1 ; AT3G59790 .
GeneIDi 825148.
KEGGi ath:AT3G59790.

Organism-specific databases

GeneFarmi 844. 89.
TAIRi AT3G59790.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q9M1Z5.
KOi K04371.
OMAi CEALAFN.
PhylomeDBi Q9M1Z5.

Enzyme and pathway databases

BioCyci ARA:AT3G59790-MONOMER.
Reactomei REACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Gene expression databases

Genevestigatori Q9M1Z5.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  4. Cited for: GENE FAMILY.
  5. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
    Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
    Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK2.

Entry informationi

Entry nameiMPK10_ARATH
AccessioniPrimary (citable) accession number: Q9M1Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3