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Protein

Mitogen-activated protein kinase 10

Gene

MPK10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89ATPPROSITE-ProRule annotation1
Active sitei186Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 74ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • auxin transport Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 10 (EC:2.7.11.24)
Short name:
AtMPK10
Short name:
MAP kinase 10
Gene namesi
Name:MPK10
Ordered Locus Names:At3g59790
ORF Names:F24G16.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G59790.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458101 – 393Mitogen-activated protein kinase 10Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphothreonineBy similarity1
Modified residuei220PhosphotyrosineBy similarity1
Modified residuei223PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9M1Z5.
PRIDEiQ9M1Z5.

PTM databases

iPTMnetiQ9M1Z5.

Expressioni

Gene expression databases

GenevisibleiQ9M1Z5. AT.

Interactioni

Subunit structurei

Interacts with MKK2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK2Q9S7U92EBI-2358527,EBI-994350
MKK9Q9FX432EBI-2358527,EBI-2128545

Protein-protein interaction databases

BioGridi10462. 4 interactors.
IntActiQ9M1Z5. 2 interactors.
STRINGi3702.AT3G59790.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M1Z5.
SMRiQ9M1Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 345Protein kinasePROSITE-ProRule annotationAdd BLAST286

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi218 – 220TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9M1Z5.
KOiK04371.
OMAiCEALAFN.
OrthoDBiEOG093613GQ.
PhylomeDBiQ9M1Z5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M1Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTNDAETL ETQGEVTTAI WPSSQILKTT IDIPGTLSHD GRYIQYNLFG
60 70 80 90 100
HIFELPAKYK PPIRPIGRGA CGIVCSAVDS ETNEKVAIKK ITQVFDNTIE
110 120 130 140 150
AKRTLREIKL LRHFDHENIV AIRDVILPPQ RDSFEDVYIV NELMEFDLYR
160 170 180 190 200
TLKSDQELTK DHGMYFMYQI LRGLKYIHSA NVLHRDLKPS NLLLSTQCDL
210 220 230 240 250
KICDFGLARA TPESNLMTEY VVTRWYRAPE LLLGSSDYTA AIDVWSVGCI
260 270 280 290 300
FMEIMNREPL FPGKDQVNQL RLLLELIGTP SEEELGSLSE YAKRYIRQLP
310 320 330 340 350
TLPRQSFTEK FPNVPPLAID LVEKMLTFDP KQRISVKEAL AHPYLSSFHD
360 370 380 390
ITDEPECSEP FNFDLDEHPF SEEQFRELIY CEALAFNPET SND
Length:393
Mass (Da):45,174
Last modified:October 1, 2000 - v1
Checksum:iF3BB2395C43CB38E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138647 Genomic DNA. Translation: CAB75798.1.
CP002686 Genomic DNA. Translation: AEE79969.1.
PIRiT47803.
RefSeqiNP_191538.1. NM_115841.2.

Genome annotation databases

EnsemblPlantsiAT3G59790.1; AT3G59790.1; AT3G59790.
GeneIDi825148.
GrameneiAT3G59790.1; AT3G59790.1; AT3G59790.
KEGGiath:AT3G59790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138647 Genomic DNA. Translation: CAB75798.1.
CP002686 Genomic DNA. Translation: AEE79969.1.
PIRiT47803.
RefSeqiNP_191538.1. NM_115841.2.

3D structure databases

ProteinModelPortaliQ9M1Z5.
SMRiQ9M1Z5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10462. 4 interactors.
IntActiQ9M1Z5. 2 interactors.
STRINGi3702.AT3G59790.1.

PTM databases

iPTMnetiQ9M1Z5.

Proteomic databases

PaxDbiQ9M1Z5.
PRIDEiQ9M1Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G59790.1; AT3G59790.1; AT3G59790.
GeneIDi825148.
GrameneiAT3G59790.1; AT3G59790.1; AT3G59790.
KEGGiath:AT3G59790.

Organism-specific databases

TAIRiAT3G59790.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9M1Z5.
KOiK04371.
OMAiCEALAFN.
OrthoDBiEOG093613GQ.
PhylomeDBiQ9M1Z5.

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9M1Z5.

Gene expression databases

GenevisibleiQ9M1Z5. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK10_ARATH
AccessioniPrimary (citable) accession number: Q9M1Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.