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Q9M1Z5

- MPK10_ARATH

UniProt

Q9M1Z5 - MPK10_ARATH

Protein

Mitogen-activated protein kinase 10

Gene

MPK10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891ATPPROSITE-ProRule annotation
    Active sitei186 – 1861Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi66 – 749ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. MAPK cascade Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G59790-MONOMER.
    ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 10 (EC:2.7.11.24)
    Short name:
    AtMPK10
    Short name:
    MAP kinase 10
    Gene namesi
    Name:MPK10
    Ordered Locus Names:At3g59790
    ORF Names:F24G16.60
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G59790.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Mitogen-activated protein kinase 10PRO_0000245810Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei218 – 2181PhosphothreonineBy similarity
    Modified residuei220 – 2201PhosphotyrosineBy similarity
    Modified residuei223 – 2231PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9M1Z5.
    PRIDEiQ9M1Z5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9M1Z5.

    Interactioni

    Subunit structurei

    Interacts with MKK2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MKK2Q9S7U92EBI-2358527,EBI-994350
    MKK9Q9FX432EBI-2358527,EBI-2128545

    Protein-protein interaction databases

    IntActiQ9M1Z5. 2 interactions.
    STRINGi3702.AT3G59790.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M1Z5.
    SMRiQ9M1Z5. Positions 35-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini60 – 345286Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi218 – 2203TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9M1Z5.
    KOiK04371.
    OMAiCEALAFN.
    PhylomeDBiQ9M1Z5.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9M1Z5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPTNDAETL ETQGEVTTAI WPSSQILKTT IDIPGTLSHD GRYIQYNLFG    50
    HIFELPAKYK PPIRPIGRGA CGIVCSAVDS ETNEKVAIKK ITQVFDNTIE 100
    AKRTLREIKL LRHFDHENIV AIRDVILPPQ RDSFEDVYIV NELMEFDLYR 150
    TLKSDQELTK DHGMYFMYQI LRGLKYIHSA NVLHRDLKPS NLLLSTQCDL 200
    KICDFGLARA TPESNLMTEY VVTRWYRAPE LLLGSSDYTA AIDVWSVGCI 250
    FMEIMNREPL FPGKDQVNQL RLLLELIGTP SEEELGSLSE YAKRYIRQLP 300
    TLPRQSFTEK FPNVPPLAID LVEKMLTFDP KQRISVKEAL AHPYLSSFHD 350
    ITDEPECSEP FNFDLDEHPF SEEQFRELIY CEALAFNPET SND 393
    Length:393
    Mass (Da):45,174
    Last modified:October 1, 2000 - v1
    Checksum:iF3BB2395C43CB38E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138647 Genomic DNA. Translation: CAB75798.1.
    CP002686 Genomic DNA. Translation: AEE79969.1.
    PIRiT47803.
    RefSeqiNP_191538.1. NM_115841.1.
    UniGeneiAt.54009.

    Genome annotation databases

    EnsemblPlantsiAT3G59790.1; AT3G59790.1; AT3G59790.
    GeneIDi825148.
    KEGGiath:AT3G59790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138647 Genomic DNA. Translation: CAB75798.1 .
    CP002686 Genomic DNA. Translation: AEE79969.1 .
    PIRi T47803.
    RefSeqi NP_191538.1. NM_115841.1.
    UniGenei At.54009.

    3D structure databases

    ProteinModelPortali Q9M1Z5.
    SMRi Q9M1Z5. Positions 35-388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9M1Z5. 2 interactions.
    STRINGi 3702.AT3G59790.1-P.

    Proteomic databases

    PaxDbi Q9M1Z5.
    PRIDEi Q9M1Z5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G59790.1 ; AT3G59790.1 ; AT3G59790 .
    GeneIDi 825148.
    KEGGi ath:AT3G59790.

    Organism-specific databases

    GeneFarmi 844. 89.
    TAIRi AT3G59790.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9M1Z5.
    KOi K04371.
    OMAi CEALAFN.
    PhylomeDBi Q9M1Z5.

    Enzyme and pathway databases

    BioCyci ARA:AT3G59790-MONOMER.
    Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Gene expression databases

    Genevestigatori Q9M1Z5.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    4. Cited for: GENE FAMILY.
    5. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
      Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
      Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK2.

    Entry informationi

    Entry nameiMPK10_ARATH
    AccessioniPrimary (citable) accession number: Q9M1Z5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3