Reviewed, UniProtKB/Swiss-Prot Q9M1X9 (ASHH4_ARATH)

Names and origin

Protein names

Recommended name:
    Putative histone-lysine N-methyltransferase ASHH4
    EC=2.1.1.43
Alternative name(s):
    ASH1 homolog 4
    Protein SET DOMAIN GROUP 24

Gene names

Name:	ASHH4
Synonyms:	SDG24, SET24
Ordered Locus Names:	At3g59960
ORF Names:	F24G16.230

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subcellular location	Nucleus By similarity. Centromere By similarity. Note: Associates with centromeric constitutive heterochromatin By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 AWS domain. Contains 1 post-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Centromere Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Putative histone-lysine N-methyltransferase ASHH4		PRO_0000233373
Regions
	Domain	60 – 109	50	AWS
	Domain	110 – 232	123	SET
	Domain	234 – 250	17	Post-SET

Sequences

	Sequence		Length	Mass (Da)
	Q9M1X9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 85D73D69D8493D6A	FASTA	352	40,123
	10 20 30 40 50 60 MSSSKKGSDR NQIRKSLRKL KKQIGELEKL ESPDRLNNVK PIFIKRNIYL KKKLKKKVKD 70 80 90 100 110 120 HGIFCSCSLD PGSSTLCGSD CNCGILLSSC SSSCKCSSEC TNKPFQQRHI KKMKLVQTEK 130 140 150 160 170 180 CGYGIVADED INSGEFIIEY VGEVIDDKIC EERLWKLNHK VETNFYLCQI NWNMVIDATH 190 200 210 220 230 240 KGNKSRYINH SCSPNTEMQK WIIDGETRIG IFATRFINKG EQLTYDYQFV QFGADQDCYC 250 260 270 280 290 300 GAVCCRKKLG AKPCKTKNTT LEEAVKPVAC KVTWKTPKLL NSEVRETNLD ASGQAWNNHS 310 320 330 340 350 QRKICCRDCI GAYYTAQMKV LTLVVDIFQV MYEDGVTEII DMCREVWKVV TA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL138647 Genomic DNA. Translation: CAB75815.1.
IPI	IPI00537449.
PIR	T47820.
RefSeq	NP_191555.1.
UniGene	At.54011
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9M1X9.
Genome annotation databases
GeneID	825166.
GenomeReviews	Gene locus AT3G59960 in contig BA000014_GR.
KEGG	ath:AT3G59960.
NMPDR	fig|3702.1.peg.17354.
Organism-specific databases
TAIR	At3g59960.
Phylogenomic databases
OMA	MSKEDWE.
Enzyme and pathway databases
BRENDA	2.1.1.43. 302.
Gene expression databases
Genevestigator	Q9M1X9.
Family and domain databases
InterPro	IPR006560. AWS. IPR003616. Post-SET_Zn_bd. IPR001214. SET. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00570. AWS. 1 hit. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51215. AWS. 1 hit. PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASHH4_ARATH

Accession

Primary (citable) accession number: Q9M1X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)