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Protein

Probable glutamate carboxypeptidase AMP1

Gene

AMP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May modulate the level of one or more small signaling molecules that have a role in regulating meristem function. May play a role in balancing and restricting the meristem-promoting activity of auxin signaling (PubMed:17553903). Involved in ethylene and giberellin (GA) signaling pathways or in a parallel pathway controlling cell and hypocotyl elongation and cellular organization (PubMed:17006669). Involved in abscisic acid (ABA) signaling pathway. Plays a negative role in ABA-mediated seed germination and seedling development (PubMed:23603279). Acts in association with LAMP1 to suppress ectopic stem cell niche formation in the shoot apical meristem (SAM) indenpendently of cytokinin signaling pathway (PubMed:25673776). Modulates responses to ABA, oxidative stress and abotic stress (PubMed:23621575). Acts as negative regulator of the ABA signaling pathway to modulate freezing and drought stress responses. Mediates carbon and amino acid metabolism (PubMed:23621575, PubMed:23603279). May be involved in the acquisition and/or maintenance of seed dormancy (PubMed:21637772). Involved in the regulation of response to heat shock and plant defense (PubMed:27743891).7 Publications

Catalytic activityi

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.Curated

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi356Zinc 1; via tele nitrogen; catalyticBy similarity1
Metal bindingi366Zinc 1; catalyticBy similarity1
Metal bindingi366Zinc 2By similarity1
Active sitei403NucleophileBy similarity1
Metal bindingi404Zinc 2By similarity1
Metal bindingi432Zinc 1; catalyticBy similarity1
Metal bindingi514Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • flower development Source: TAIR
  • leaf vascular tissue pattern formation Source: TAIR
  • meristem development Source: TAIR
  • photomorphogenesis Source: TAIR
  • proteolysis Source: UniProtKB
  • regulation of floral meristem growth Source: UniProtKB
  • regulation of inflorescence meristem growth Source: UniProtKB
  • regulation of root meristem growth Source: UniProtKB
  • root development Source: TAIR

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G54720-MONOMER
ReactomeiR-ATH-70614 Amino acid synthesis and interconversion (transamination)

Protein family/group databases

MEROPSiM28.007

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate carboxypeptidase AMP1Curated (EC:3.4.17.21Curated)
Alternative name(s):
Probable glutamate carboxypeptidase 2Curated
Probable glutamate carboxypeptidase IICurated
Protein ALTERED MERISTEM PROGRAM 11 Publication
Short name:
AtAMP11 Publication
Protein CONSTITUTIVE MORPHOGENESIS 2
Protein HAUPTLINGCurated
Protein MULTIFOLIACurated
Protein PRIMORDIA TIMING
Gene namesi
Name:AMP11 Publication
Synonyms:COP2Curated, HPTCurated, MFO1Curated, PT1 Publication
Ordered Locus Names:At3g54720Imported
ORF Names:T5N23_80Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54720
TAIRilocus:2102470 AT3G54720

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicCuratedAdd BLAST24
Transmembranei25 – 42Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini43 – 705ExtracellularCuratedAdd BLAST663

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi404E → K in PT; shows pleiotropic phenotypes, including altered shoot apical meristems, increased cell proliferation, polycotyly, constitutive photomorphogenesis, early flowering time, increased levels of endogenous cytokinin, and increased cyclin cycD3 expression. 1 Publication1
Mutagenesisi545G → E in amp1-7; weak allele showing some pleiotropic phenotypes, including altered shoot apical meristem, increased cell proliferation, serrated leaves prior to full expansion, early flowering and increased levels of endogenous cytokinin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741261 – 705Probable glutamate carboxypeptidase AMP1Add BLAST705

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi137N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi322N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi676N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M1S8
PRIDEiQ9M1S8

Expressioni

Tissue specificityi

Expressed in all plant parts. Highest levels in the bolt stem, inflorescence, root and silique. Low level in leaves.1 Publication

Inductioni

Down-regulated by abscisic acid (ABA).2 Publications

Gene expression databases

ExpressionAtlasiQ9M1S8 baseline and differential
GenevisibleiQ9M1S8 AT

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO1O043792EBI-6912802,EBI-6912745

Protein-protein interaction databases

BioGridi9953, 1 interactor
IntActiQ9M1S8, 1 interactor
STRINGi3702.AT3G54720.1

Structurei

3D structure databases

ProteinModelPortaliQ9M1S8
SMRiQ9M1S8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni255 – 548CatalyticCuratedAdd BLAST294

Sequence similaritiesi

Belongs to the peptidase M28 family. M28B subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2195 Eukaryota
COG2234 LUCA
HOGENOMiHOG000211921
InParanoidiQ9M1S8
KOiK01301
OMAiYVAFQDI
OrthoDBiEOG09360AY4

Family and domain databases

Gene3Di1.20.930.40, 1 hit
InterProiView protein in InterPro
IPR003137 PA_domain
IPR007484 Peptidase_M28
IPR007365 TFR-like_dimer_dom
IPR036757 TFR-like_dimer_dom_sf
PfamiView protein in Pfam
PF02225 PA, 1 hit
PF04389 Peptidase_M28, 1 hit
PF04253 TFR_dimer, 1 hit
SUPFAMiSSF47672 SSF47672, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M1S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPLTTRPT VTGISIIPFR QPPPLCSFLF VIVLFVATFY TLHHPDAVTP
60 70 80 90 100
PLLFSRNAYN ALRLRRLFLS SASNATISSY LRELTRHPHL AGTKPSLDTL
110 120 130 140 150
HYVFNHFQSL GLETHVAEYE ALLSYPTHIS VTASFSNTTT LEFDLNDVPG
160 170 180 190 200
DSPVVRPYHA YSPSGSAQGN VVFVNHGEER DYHALESIGV SVKGCVVLAR
210 220 230 240 250
KGENLGRGAI VKIAEAKGAL GVLIYAENDG GGFGGIERGT VMRGIGDPVS
260 270 280 290 300
PGWPGVVGGE KLSLDDELVT RRFPKIPSLP LSLRNAEIIL ASLGGARAPL
310 320 330 340 350
EWRNSGRVGP GQRVGPGRMV INMTFQGEMK MKKINNVVVT IRGSEEADRY
360 370 380 390 400
VILGNHRDAW TYGAVDPNSG TSALLDISRR FALLLKSGWR PRRTILLCSW
410 420 430 440 450
DAEEFGMIGS TEWIEENVLN LGASAVAYLN VDCAVQGSGF FAGATPQLDG
460 470 480 490 500
LLVDVLKLVQ DPDAVGLTVE ETFKSQNNII QRLSRVDSDF SGFLHHAGIP
510 520 530 540 550
SIDMYYGADY PVYHTAFDSY DWMIHNADPL FHRHVAMAGI WGLLGILLAD
560 570 580 590 600
EPLIPFDYIS YADQLQAHRD KLSKLLEGKV SVNPLSMAIQ EFSLVAKEAA
610 620 630 640 650
DEAKKLKGKS YSKNDVAAAA KRRELNDRLM LVERGFLDAE GIKGKEWFKH
660 670 680 690 700
LVYGPAAEPE SKLGFFPGIA DAIAMNASEG IIEHEIWRVA RAIQRASKAL

KGGFT
Length:705
Mass (Da):77,153
Last modified:May 3, 2011 - v3
Checksum:i8F4310FA19F5C22B
GO

Sequence cautioni

The sequence CAB77592 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti563D → E in AAL03993 (PubMed:11549767).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF357217 mRNA Translation: AAL03993.1
AL138650 Genomic DNA Translation: CAB77592.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE79270.1
BT046200 mRNA Translation: ACI49799.1
PIRiT47631
RefSeqiNP_567007.1, NM_115329.3
UniGeneiAt.1213

Genome annotation databases

EnsemblPlantsiAT3G54720.1; AT3G54720.1; AT3G54720
GeneIDi824637
GrameneiAT3G54720.1; AT3G54720.1; AT3G54720
KEGGiath:AT3G54720

Similar proteinsi

Entry informationi

Entry nameiGCP2_ARATH
AccessioniPrimary (citable) accession number: Q9M1S8
Secondary accession number(s): B5X581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health