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Protein

Probable glutamate carboxypeptidase 2

Gene

AMP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May modulate the level of one or more small signaling molecules that have a role in regulating meristem function. May play a role in balancing and restricting the meristem-promoting activity of auxin signaling.1 Publication

Catalytic activityi

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi356 – 3561Zinc 1By similarity
Metal bindingi366 – 3661Zinc 1By similarity
Metal bindingi366 – 3661Zinc 2By similarity
Active sitei403 – 4031NucleophileBy similarity
Metal bindingi404 – 4041Zinc 2By similarity
Metal bindingi432 – 4321Zinc 1By similarity
Metal bindingi514 – 5141Zinc 2By similarity

GO - Molecular functioni

  • carboxypeptidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • flower development Source: TAIR
  • leaf vascular tissue pattern formation Source: TAIR
  • meristem development Source: TAIR
  • photomorphogenesis Source: TAIR
  • proteolysis Source: UniProtKB
  • regulation of floral meristem growth Source: UniProtKB
  • regulation of inflorescence meristem growth Source: UniProtKB
  • regulation of root meristem growth Source: UniProtKB
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G54720-MONOMER.
ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).

Protein family/group databases

MEROPSiM28.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate carboxypeptidase 2 (EC:3.4.17.21)
Alternative name(s):
Probable glutamate carboxypeptidase II
Gene namesi
Name:AMP1
Ordered Locus Names:At3g54720
ORF Names:T5N23_80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54720.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence analysisAdd
BLAST
Transmembranei25 – 4218Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini43 – 705663ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi404 – 4041E → K in PT; show pleiotropic phenotypes, including altered shoot apical meristems, increased cell proliferation, polycotyly, constitutive photomorphogenesis, early flowering time, increased levels of endogenous cytokinin, and increased cyclin cycD3 expression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Probable glutamate carboxypeptidase 2PRO_0000174126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence analysis
Glycosylationi676 – 6761N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M1S8.
PRIDEiQ9M1S8.

Expressioni

Tissue specificityi

Expressed in all plant parts. Highest levels in the bolt stem, inflorescence, root and silique. Low level in leaves.

Gene expression databases

ExpressionAtlasiQ9M1S8. baseline and differential.
GenevisibleiQ9M1S8. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO1O043792EBI-6912802,EBI-6912745

Protein-protein interaction databases

BioGridi9953. 1 interaction.
IntActiQ9M1S8. 1 interaction.
STRINGi3702.AT3G54720.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M1S8.
SMRiQ9M1S8. Positions 64-673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni255 – 548294CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M28 family. M28B subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
HOGENOMiHOG000211921.
InParanoidiQ9M1S8.
KOiK01301.
OMAiWIPRRTI.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M1S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPLTTRPT VTGISIIPFR QPPPLCSFLF VIVLFVATFY TLHHPDAVTP
60 70 80 90 100
PLLFSRNAYN ALRLRRLFLS SASNATISSY LRELTRHPHL AGTKPSLDTL
110 120 130 140 150
HYVFNHFQSL GLETHVAEYE ALLSYPTHIS VTASFSNTTT LEFDLNDVPG
160 170 180 190 200
DSPVVRPYHA YSPSGSAQGN VVFVNHGEER DYHALESIGV SVKGCVVLAR
210 220 230 240 250
KGENLGRGAI VKIAEAKGAL GVLIYAENDG GGFGGIERGT VMRGIGDPVS
260 270 280 290 300
PGWPGVVGGE KLSLDDELVT RRFPKIPSLP LSLRNAEIIL ASLGGARAPL
310 320 330 340 350
EWRNSGRVGP GQRVGPGRMV INMTFQGEMK MKKINNVVVT IRGSEEADRY
360 370 380 390 400
VILGNHRDAW TYGAVDPNSG TSALLDISRR FALLLKSGWR PRRTILLCSW
410 420 430 440 450
DAEEFGMIGS TEWIEENVLN LGASAVAYLN VDCAVQGSGF FAGATPQLDG
460 470 480 490 500
LLVDVLKLVQ DPDAVGLTVE ETFKSQNNII QRLSRVDSDF SGFLHHAGIP
510 520 530 540 550
SIDMYYGADY PVYHTAFDSY DWMIHNADPL FHRHVAMAGI WGLLGILLAD
560 570 580 590 600
EPLIPFDYIS YADQLQAHRD KLSKLLEGKV SVNPLSMAIQ EFSLVAKEAA
610 620 630 640 650
DEAKKLKGKS YSKNDVAAAA KRRELNDRLM LVERGFLDAE GIKGKEWFKH
660 670 680 690 700
LVYGPAAEPE SKLGFFPGIA DAIAMNASEG IIEHEIWRVA RAIQRASKAL

KGGFT
Length:705
Mass (Da):77,153
Last modified:May 3, 2011 - v3
Checksum:i8F4310FA19F5C22B
GO

Sequence cautioni

The sequence CAB77592.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti563 – 5631D → E in AAL03993 (PubMed:11549767).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF357217 mRNA. Translation: AAL03993.1.
AL138650 Genomic DNA. Translation: CAB77592.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79270.1.
PIRiT47631.
RefSeqiNP_567007.1. NM_115329.2.
UniGeneiAt.1213.

Genome annotation databases

EnsemblPlantsiAT3G54720.1; AT3G54720.1; AT3G54720.
GeneIDi824637.
GrameneiAT3G54720.1; AT3G54720.1; AT3G54720.
KEGGiath:AT3G54720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF357217 mRNA. Translation: AAL03993.1.
AL138650 Genomic DNA. Translation: CAB77592.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79270.1.
PIRiT47631.
RefSeqiNP_567007.1. NM_115329.2.
UniGeneiAt.1213.

3D structure databases

ProteinModelPortaliQ9M1S8.
SMRiQ9M1S8. Positions 64-673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9953. 1 interaction.
IntActiQ9M1S8. 1 interaction.
STRINGi3702.AT3G54720.1.

Protein family/group databases

MEROPSiM28.007.

Proteomic databases

PaxDbiQ9M1S8.
PRIDEiQ9M1S8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54720.1; AT3G54720.1; AT3G54720.
GeneIDi824637.
GrameneiAT3G54720.1; AT3G54720.1; AT3G54720.
KEGGiath:AT3G54720.

Organism-specific databases

TAIRiAT3G54720.

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
HOGENOMiHOG000211921.
InParanoidiQ9M1S8.
KOiK01301.
OMAiWIPRRTI.

Enzyme and pathway databases

BioCyciARA:AT3G54720-MONOMER.
ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ9M1S8.

Gene expression databases

ExpressionAtlasiQ9M1S8. baseline and differential.
GenevisibleiQ9M1S8. AT.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis AMP1 gene encodes a putative glutamate carboxypeptidase."
    Helliwell C.A., Chin-Atkins A.N., Wilson I.W., Chapple R., Dennis E.S., Chaudhury A.
    Plant Cell 13:2115-2125(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLU-404.
    Strain: cv. Landsberg erecta.
    Tissue: Silique.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "AMP1 and MP antagonistically regulate embryo and meristem development in Arabidopsis."
    Vidaurre D.P., Ploense S., Krogan N.T., Berleth T.
    Development 134:2561-2567(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGCP2_ARATH
AccessioniPrimary (citable) accession number: Q9M1S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: May 3, 2011
Last modified: May 11, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.