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Protein

Probable protein S-acyltransferase 4

Gene

PAT04

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei176S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • root hair elongation Source: TAIR

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 4 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At3g56930
Zinc finger DHHC domain-containing protein At3g56930
Gene namesi
Name:PAT04
Ordered Locus Names:At3g56930
ORF Names:F24I3.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G56930.
TAIRilocus:2080555. AT3G56930.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei68 – 88HelicalSequence analysisAdd BLAST21
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • root hair Source: TAIR
  • trans-Golgi network transport vesicle Source: TAIR

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003635991 – 477Probable protein S-acyltransferase 4Add BLAST477

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9M1K5.

PTM databases

iPTMnetiQ9M1K5.

Expressioni

Gene expression databases

ExpressionAtlasiQ9M1K5. baseline and differential.
GenevisibleiQ9M1K5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G56930.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 196DHHCPROSITE-ProRule annotationAdd BLAST51

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi313 – 316Poly-Met4
Compositional biasi380 – 390His-richAdd BLAST11

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000242943.
InParanoidiQ9M1K5.
KOiK16675.
OMAiHMDEKIS.
OrthoDBiEOG09360EBP.
PhylomeDBiQ9M1K5.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9M1K5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWNETKLKR LYQVWRGSNK FLCGGRLIFG PDASSLYLST ILILGPAVMF
60 70 80 90 100
FVKMYTKMAD PRTKNPNLCI PILCVSWILT ILDIFFLLMT SSRDPGIVPR
110 120 130 140 150
SFRPPETDDA PDSTTPSMEW VSGRTPNIRI PRVKDVTVNG HTVKVKFCDT
160 170 180 190 200
CLLYRPPRAS HCSICNNCVQ RFDHHCPWVG QCIGVRNYRF FFMFISTSTT
210 220 230 240 250
LCIYVFAFSW LNIFQRHMDE KISIWKAISK DVLSDILIVY CFITVWFVGG
260 270 280 290 300
LTIFHSYLIC TNQTTYENFR YRYDKKENPY NKGILGNIWE IFLSKIPPSM
310 320 330 340 350
NKFRSFVKEE DYMMMMVETP TSNLGESLVS SKEKIDIEMG GGRIVDESGK
360 370 380 390 400
SYSLPEILRN LNYEDLEDDC EEDDLKAKDH HHHHHHQHQH NEGIIPPFDP
410 420 430 440 450
FFTNEIGSNK DERNGEESGG SSSDGENTGK RVRVSDEDEE KVEGYERNWS
460 470
TDKGMNINAG SEDGASSPVS TSPMLRK
Length:477
Mass (Da):54,777
Last modified:October 1, 2000 - v1
Checksum:i132CE8502333C5FB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti466S → I in AAM20704 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138655 Genomic DNA. Translation: CAB72163.1.
CP002686 Genomic DNA. Translation: AEE79587.1.
AK117663 mRNA. Translation: BAC42316.1.
AY099853 mRNA. Translation: AAM20704.1.
BT008533 mRNA. Translation: AAP40360.1.
PIRiT47753.
RefSeqiNP_191252.1. NM_115552.4. [Q9M1K5-1]
UniGeneiAt.34882.

Genome annotation databases

EnsemblPlantsiAT3G56930.1; AT3G56930.1; AT3G56930. [Q9M1K5-1]
GeneIDi824860.
GrameneiAT3G56930.1; AT3G56930.1; AT3G56930. [Q9M1K5-1]
KEGGiath:AT3G56930.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiZDH13_ARATH
AccessioniPrimary (citable) accession number: Q9M1K5
Secondary accession number(s): Q8L5Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families