Reviewed, UniProtKB/Swiss-Prot Q9M1K2 (PI5K4_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4-phosphate 5-kinase 4
      Short name=AtPIP5K4
    EC=2.7.1.68
Alternative name(s):
    1-phosphatidylinositol-4-phosphate kinase 4
    PtdIns(4)P-5-kinase 4
    Diphosphoinositide kinase 4

Gene names

Name:	PIP5K4
Ordered Locus Names:	At3g56960
ORF Names:	F24I3.40

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Sequence similarities	Contains 8 MORN repeats. Contains 1 PIPK domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from mutant phenotype. Source: TAIR establishment of tissue polarity Inferred from mutant phenotype. Source: TAIR phosphatidylinositol metabolic process Inferred from direct assay. Source: TAIR plant-type cell wall modification Inferred from mutant phenotype. Source: TAIR pollen germination Inferred from genetic interaction. Source: TAIR pollen tube growth Inferred from mutant phenotype. Source: TAIR stomatal movement Inferred from mutant phenotype. Source: TAIR
Cellular component	apical plasma membrane Inferred from direct assay. Source: TAIR cytosolic part Inferred from direct assay. Source: TAIR
Molecular function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from direct assay. Source: TAIR ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Phosphatidylinositol-4-phosphate 5-kinase 4		PRO_0000185476
Regions
	Repeat	77 – 99	23	MORN 1
	Repeat	100 – 122	23	MORN 2
	Repeat	123 – 145	23	MORN 3
	Repeat	146 – 168	23	MORN 4
	Repeat	169 – 191	23	MORN 5
	Repeat	192 – 214	23	MORN 6
	Repeat	215 – 237	23	MORN 7
	Repeat	238 – 259	22	MORN 8
	Domain	382 – 775	394	PIPK
	Region	735 – 756	22	Activation loop By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9M1K2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FAEB3BF1603C18B0	FASTA	779	89,238
	10 20 30 40 50 60 MSKEQSCVLK AWEVTVRKTQ QAKKRANSIF GTVSVAPQTD DDATTTTEEN DDETSTNRSS 70 80 90 100 110 120 IGELYHAERI LPNGDYYTGQ WYDSFPHGHG KYLWTDGCMY IGDWYNGKTM GRGKFGWPSG 130 140 150 160 170 180 ATYEGEFKSG YMDGVGLYTG PSGDTYKGQW VMNLKHGHGI KRFANGDVYD GEWRRGLQEA 190 200 210 220 230 240 QGKYQWRDGS YYMGEWKNAT ICGKGTFIWT DGNRYDGFWD DGFPRGNGTF KWADGSFYVG 250 260 270 280 290 300 HWSNDPEEMN GTYYPSGDDG SPEWDPKDVF TNLSEYKICS GERVPVLPSQ KKLSVWNSSK 310 320 330 340 350 360 RVEKPRRMSV DGRVSVGVDR AFEKMNMWGT ESGEGAADID STTRRDLDAE MMRLEAEGFI 370 380 390 400 410 420 QSLRPSPAPM RLPRAGRKQG ETISKGHRNY ELMLNLQLGI RHAVGKQAPV VSLDLKHSAF 430 440 450 460 470 480 DPKEKVWTRF PPEGTKYTPP HQSSEFKWKD YCPLVFRSLR KLFKVDPADY MLSICGNDAL 490 500 510 520 530 540 RELSSPGKSG SFFYLTNDDR YMIKTMKKSE TKVLLRMLAA YYNHVRAFEN TLVIRFYGLH 550 560 570 580 590 600 CVKLTGPIQK KVRFVIMGNL FCSEYSIHRR FDLKGSSLGR TTDKPESEIN SNTILKDLDL 610 620 630 640 650 660 NFIFRLQKAW YQEFIRQVDK DCEFLEQERI MDYSLLVGIH FREASVAGEL IPSGARTPIG 670 680 690 700 710 720 EFEDESAPRL SRADVDQLLS DPTRWASIRL GGNMPARAER TMRRSDCEFQ LVGEPTGEYY 730 740 750 760 770 EVVMIFGIID ILQDYDISKK LEHAYKSIQY DPTSISAVDP RLYSRRFRDF IFKVFTEDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL138655 Genomic DNA. Translation: CAB72166.1.
IPI	IPI00523818.
PIR	T47756.
RefSeq	NP_191255.1.
UniGene	At.53961
3D structure databases
SMR	Q9M1K2. Positions 95-205, 388-776.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9M1K2.
Proteomic databases
PRIDE	Q9M1K2.
ProMEX	Q9M1K2.
Genome annotation databases
GeneID	824863.
GenomeReviews	Gene locus AT3G56960 in contig BA000014_GR.
KEGG	ath:AT3G56960.
NMPDR	fig|3702.1.peg.17025.
Organism-specific databases
TAIR	At3g56960.
Phylogenomic databases
eggNOG	KOG0229.
HOGENOM	HBG588608.
InParanoid	Q9M1K2.
OMA	RRMSVDG.
PhylomeDB	Q9M1K2.
Enzyme and pathway databases
BRENDA	2.7.1.68. 302.
Gene expression databases
Genevestigator	Q9M1K2.
GermOnline	AT3G56960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003409. MORN. IPR017163. PIno-4-P-5_kinase_pln. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PIP5K. 1 hit.
Pfam	PF02493. MORN. 8 hits. PF01504. PIP5K. 1 hit. [Graphical view]
PIRSF	PIRSF037274. PIP5K_plant_prd. 1 hit.
SMART	SM00698. MORN. 7 hits. SM00330. PIPKc. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI5K4_ARATH

Accession

Primary (citable) accession number: Q9M1K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)