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Q9M1E8

- SEN21_ARATH

UniProt

Q9M1E8 - SEN21_ARATH

Protein

tRNA-splicing endonuclease subunit Sen2-1

Gene

SEN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei148 – 1481By similarity
    Active sitei156 – 1561By similarity
    Active sitei190 – 1901By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Enzyme and pathway databases

    BioCyciARA:AT3G45590-MONOMER.
    ARA:GQT-2187-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen2-1 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease Sen2-1
    Short name:
    AtSen1
    Gene namesi
    Name:SEN1
    Ordered Locus Names:At3g45590
    ORF Names:F9K21.170
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G45590.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237tRNA-splicing endonuclease subunit Sen2-1PRO_0000109456Add
    BLAST

    Expressioni

    Gene expression databases

    ArrayExpressiQ9M1E8.
    GenevestigatoriQ9M1E8.

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M1E8.
    SMRiQ9M1E8. Positions 51-198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000030044.
    InParanoidiQ9M1E8.
    KOiK15322.
    OMAiLRSKNWI.
    PhylomeDBiQ9M1E8.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9M1E8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES    50
    EEAELLDRCC FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS 100
    LENEVDLWRS MSSFKQDFAI LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH 150
    HPSLVHSEYA VLVQSIGGND RLKVWSDIHC SVRLTGSVAK SLLVLYVNRK 200
    VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT 237
    Length:237
    Mass (Da):27,359
    Last modified:October 1, 2000 - v1
    Checksum:i2844856CB58CD0B2
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241S → L in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti27 – 271R → Q in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti52 – 521E → Q in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti67 – 671G → S in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti167 – 1671G → S in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti191 – 1911S → T in strain: cv. Landsberg erecta. 1 Publication
    Natural varianti199 – 2057RKVNTEK → GQVKTEN in strain: cv. Landsberg erecta.
    Natural varianti213 – 2142ED → DE in strain: cv. Landsberg erecta.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB036339 mRNA. Translation: BAA88627.1.
    AB036340 Genomic DNA. Translation: BAA88628.1.
    AL138657 Genomic DNA. Translation: CAB75488.1.
    CP002686 Genomic DNA. Translation: AEE78047.1.
    CP002686 Genomic DNA. Translation: AEE78048.1.
    AK221492 mRNA. Translation: BAD94688.1.
    PIRiT47499.
    RefSeqiNP_001078245.1. NM_001084776.1.
    NP_190145.1. NM_114428.3.
    UniGeneiAt.22349.
    At.70243.

    Genome annotation databases

    EnsemblPlantsiAT3G45590.1; AT3G45590.1; AT3G45590.
    AT3G45590.2; AT3G45590.2; AT3G45590.
    GeneIDi823701.
    KEGGiath:AT3G45590.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB036339 mRNA. Translation: BAA88627.1 .
    AB036340 Genomic DNA. Translation: BAA88628.1 .
    AL138657 Genomic DNA. Translation: CAB75488.1 .
    CP002686 Genomic DNA. Translation: AEE78047.1 .
    CP002686 Genomic DNA. Translation: AEE78048.1 .
    AK221492 mRNA. Translation: BAD94688.1 .
    PIRi T47499.
    RefSeqi NP_001078245.1. NM_001084776.1.
    NP_190145.1. NM_114428.3.
    UniGenei At.22349.
    At.70243.

    3D structure databases

    ProteinModelPortali Q9M1E8.
    SMRi Q9M1E8. Positions 51-198.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G45590.1 ; AT3G45590.1 ; AT3G45590 .
    AT3G45590.2 ; AT3G45590.2 ; AT3G45590 .
    GeneIDi 823701.
    KEGGi ath:AT3G45590.

    Organism-specific databases

    GeneFarmi 5164. 493.
    TAIRi AT3G45590.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000030044.
    InParanoidi Q9M1E8.
    KOi K15322.
    OMAi LRSKNWI.
    PhylomeDBi Q9M1E8.

    Enzyme and pathway databases

    BioCyci ARA:AT3G45590-MONOMER.
    ARA:GQT-2187-MONOMER.

    Gene expression databases

    ArrayExpressi Q9M1E8.
    Genevestigatori Q9M1E8.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
      Akama K., Junker V., Beier H.
      Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS LEU-24; GLN-27; GLN-52; SER-67; SER-167; THR-191; 199-GLY--ASN-205 AND 213-ASP-GLU-214.
      Strain: cv. Columbia and cv. Landsberg erecta.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiSEN21_ARATH
    AccessioniPrimary (citable) accession number: Q9M1E8
    Secondary accession number(s): Q9S7L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3