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Protein

tRNA-splicing endonuclease subunit Sen2-1

Gene

SEN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148By similarity1
Active sitei156By similarity1
Active sitei190By similarity1

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • tRNA-type intron splice site recognition and cleavage Source: GO_Central
  • zygote asymmetric cytokinesis in embryo sac Source: TAIR

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Enzyme and pathway databases

BRENDAi4.6.1.16 399

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2-1 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2-1
Short name:
AtSen1
Gene namesi
Name:SEN1
Ordered Locus Names:At3g45590
ORF Names:F9K21.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G45590
TAIRilocus:2085687 AT3G45590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094561 – 237tRNA-splicing endonuclease subunit Sen2-1Add BLAST237

Proteomic databases

PaxDbiQ9M1E8
PRIDEiQ9M1E8

Expressioni

Gene expression databases

ExpressionAtlasiQ9M1E8 baseline and differential
GenevisibleiQ9M1E8 AT

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G45590.1

Structurei

3D structure databases

ProteinModelPortaliQ9M1E8
SMRiQ9M1E8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4685 Eukaryota
COG1676 LUCA
HOGENOMiHOG000030044
InParanoidiQ9M1E8
KOiK15322
OMAiVYKLKFR
OrthoDBiEOG09360PFO
PhylomeDBiQ9M1E8

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR006676 tRNA_splic
IPR016589 tRNA_splic_SEN2
PANTHERiPTHR21227 PTHR21227, 2 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF011789 tRNA_splic_SEN2, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M1E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES
60 70 80 90 100
EEAELLDRCC FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS
110 120 130 140 150
LENEVDLWRS MSSFKQDFAI LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH
160 170 180 190 200
HPSLVHSEYA VLVQSIGGND RLKVWSDIHC SVRLTGSVAK SLLVLYVNRK
210 220 230
VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT
Length:237
Mass (Da):27,359
Last modified:October 1, 2000 - v1
Checksum:i2844856CB58CD0B2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti24S → L in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti27R → Q in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti52E → Q in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti67G → S in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti167G → S in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti191S → T in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti199 – 205RKVNTEK → GQVKTEN in strain: cv. Landsberg erecta. 7
Natural varianti213 – 214ED → DE in strain: cv. Landsberg erecta. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036339 mRNA Translation: BAA88627.1
AB036340 Genomic DNA Translation: BAA88628.1
AL138657 Genomic DNA Translation: CAB75488.1
CP002686 Genomic DNA Translation: AEE78047.1
CP002686 Genomic DNA Translation: AEE78048.1
AK221492 mRNA Translation: BAD94688.1
PIRiT47499
RefSeqiNP_001078245.1, NM_001084776.2
NP_190145.1, NM_114428.4
UniGeneiAt.22349
At.70243

Genome annotation databases

EnsemblPlantsiAT3G45590.1; AT3G45590.1; AT3G45590
AT3G45590.2; AT3G45590.2; AT3G45590
GeneIDi823701
GrameneiAT3G45590.1; AT3G45590.1; AT3G45590
AT3G45590.2; AT3G45590.2; AT3G45590
KEGGiath:AT3G45590

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSEN21_ARATH
AccessioniPrimary (citable) accession number: Q9M1E8
Secondary accession number(s): Q9S7L3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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