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Q9M1E8

- SEN21_ARATH

UniProt

Q9M1E8 - SEN21_ARATH

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Protein
tRNA-splicing endonuclease subunit Sen2-1
Gene
SEN1, At3g45590, F9K21.170
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei148 – 1481 By similarity
Active sitei156 – 1561 By similarity
Active sitei190 – 1901 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

mRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciARA:AT3G45590-MONOMER.
ARA:GQT-2187-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2-1 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2-1
Short name:
AtSen1
Gene namesi
Name:SEN1
Ordered Locus Names:At3g45590
ORF Names:F9K21.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G45590.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA-splicing endonuclease subunit Sen2-1
PRO_0000109456Add
BLAST

Expressioni

Gene expression databases

ArrayExpressiQ9M1E8.
GenevestigatoriQ9M1E8.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9M1E8.
SMRiQ9M1E8. Positions 51-198.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000030044.
InParanoidiQ9M1E8.
KOiK15322.
OMAiLRSKNWI.
PhylomeDBiQ9M1E8.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M1E8-1 [UniParc]FASTAAdd to Basket

« Hide

MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES    50
EEAELLDRCC FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS 100
LENEVDLWRS MSSFKQDFAI LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH 150
HPSLVHSEYA VLVQSIGGND RLKVWSDIHC SVRLTGSVAK SLLVLYVNRK 200
VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT 237
Length:237
Mass (Da):27,359
Last modified:October 1, 2000 - v1
Checksum:i2844856CB58CD0B2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241S → L in strain: cv. Landsberg erecta. 1 Publication
Natural varianti27 – 271R → Q in strain: cv. Landsberg erecta. 1 Publication
Natural varianti52 – 521E → Q in strain: cv. Landsberg erecta. 1 Publication
Natural varianti67 – 671G → S in strain: cv. Landsberg erecta. 1 Publication
Natural varianti167 – 1671G → S in strain: cv. Landsberg erecta. 1 Publication
Natural varianti191 – 1911S → T in strain: cv. Landsberg erecta. 1 Publication
Natural varianti199 – 2057RKVNTEK → GQVKTEN in strain: cv. Landsberg erecta.
Natural varianti213 – 2142ED → DE in strain: cv. Landsberg erecta.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB036339 mRNA. Translation: BAA88627.1.
AB036340 Genomic DNA. Translation: BAA88628.1.
AL138657 Genomic DNA. Translation: CAB75488.1.
CP002686 Genomic DNA. Translation: AEE78047.1.
CP002686 Genomic DNA. Translation: AEE78048.1.
AK221492 mRNA. Translation: BAD94688.1.
PIRiT47499.
RefSeqiNP_001078245.1. NM_001084776.1.
NP_190145.1. NM_114428.3.
UniGeneiAt.22349.
At.70243.

Genome annotation databases

EnsemblPlantsiAT3G45590.1; AT3G45590.1; AT3G45590.
AT3G45590.2; AT3G45590.2; AT3G45590.
GeneIDi823701.
KEGGiath:AT3G45590.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB036339 mRNA. Translation: BAA88627.1 .
AB036340 Genomic DNA. Translation: BAA88628.1 .
AL138657 Genomic DNA. Translation: CAB75488.1 .
CP002686 Genomic DNA. Translation: AEE78047.1 .
CP002686 Genomic DNA. Translation: AEE78048.1 .
AK221492 mRNA. Translation: BAD94688.1 .
PIRi T47499.
RefSeqi NP_001078245.1. NM_001084776.1.
NP_190145.1. NM_114428.3.
UniGenei At.22349.
At.70243.

3D structure databases

ProteinModelPortali Q9M1E8.
SMRi Q9M1E8. Positions 51-198.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G45590.1 ; AT3G45590.1 ; AT3G45590 .
AT3G45590.2 ; AT3G45590.2 ; AT3G45590 .
GeneIDi 823701.
KEGGi ath:AT3G45590.

Organism-specific databases

GeneFarmi 5164. 493.
TAIRi AT3G45590.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000030044.
InParanoidi Q9M1E8.
KOi K15322.
OMAi LRSKNWI.
PhylomeDBi Q9M1E8.

Enzyme and pathway databases

BioCyci ARA:AT3G45590-MONOMER.
ARA:GQT-2187-MONOMER.

Gene expression databases

ArrayExpressi Q9M1E8.
Genevestigatori Q9M1E8.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
    Akama K., Junker V., Beier H.
    Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS LEU-24; GLN-27; GLN-52; SER-67; SER-167; THR-191; 199-GLY--ASN-205 AND 213-ASP-GLU-214.
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiSEN21_ARATH
AccessioniPrimary (citable) accession number: Q9M1E8
Secondary accession number(s): Q9S7L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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