Q9M1E8 (SEN21_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 75.
History...
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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: tRNA-splicing endonuclease subunit Sen2-1 EC=3.1.27.9 Alternative name(s): tRNA-intron endonuclease Sen2-1 Short name=AtSen1 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 237 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity. |
| Catalytic activity | Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. |
| Subunit structure | tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity. |
| Subcellular location | Nucleus By similarity. |
| Sequence similarities | Belongs to the tRNA-intron endonuclease family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing tRNA processing |
| Cellular component | Nucleus |
| Coding sequence diversity | Polymorphism |
| Molecular function | Endonuclease Hydrolase Nuclease |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysisInferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligationInferred from electronic annotation. Source: InterPro |
| Cellular_component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 237 | 237 | tRNA-splicing endonuclease subunit Sen2-1 | PRO_0000109456 | |||||
Sites | |||||||||
| Active site | 148 | 1 | By similarity | ||||||
| Active site | 156 | 1 | By similarity | ||||||
| Active site | 190 | 1 | By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 24 | 1 | S → L in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 27 | 1 | R → Q in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 52 | 1 | E → Q in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 67 | 1 | G → S in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 167 | 1 | G → S in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 191 | 1 | S → T in strain: cv. Landsberg erecta. Ref.1 | ||||||
| Natural variant | 199 – 205 | 7 | RKVNTEK → GQVKTEN in strain: cv. Landsberg erecta. | ||||||
| Natural variant | 213 – 214 | 2 | ED → DE in strain: cv. Landsberg erecta. | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana." Akama K., Junker V., Beier H. Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS LEU-24; GLN-27; GLN-52; SER-67; SER-167; THR-191; 199-GLY--ASN-205 AND 213-ASP-GLU-214. Strain: cv. Columbia and cv. Landsberg erecta. |
| [2] | "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.  Tabata S.Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB036339 mRNA. Translation: BAA88627.1. AB036340 Genomic DNA. Translation: BAA88628.1. AL138657 Genomic DNA. Translation: CAB75488.1. CP002686 Genomic DNA. Translation: AEE78047.1. CP002686 Genomic DNA. Translation: AEE78048.1. AK221492 mRNA. Translation: BAD94688.1. |
| IPI | IPI00525737. |
| PIR | T47499. |
| RefSeq | NP_001078245.1. NM_001084776.1. NP_190145.1. NM_114428.3. |
| UniGene | At.22349. At.70243. |
3D structure databases | |
| ProteinModelPortal | Q9M1E8. |
| SMR | Q9M1E8. Positions 51-198. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT3G45590.1; AT3G45590.1; AT3G45590. AT3G45590.2; AT3G45590.2; AT3G45590. |
| GeneID | 823701. |
| KEGG | ath:AT3G45590. |
Organism-specific databases | |
| GeneFarm | 5164. 493. |
| TAIR | At3g45590. |
Phylogenomic databases | |
| eggNOG | COG1676. |
| HOGENOM | HOG000030044. |
| InParanoid | Q9M1E8. |
| KO | K15322. |
| OMA | NWIVRSG. |
| PhylomeDB | Q9M1E8. |
| ProtClustDB | CLSN2684758. |
Gene expression databases | |
| ArrayExpress | Q9M1E8. |
| Genevestigator | Q9M1E8. |
| GermOnline | AT3G45590. Arabidopsis thaliana. |
Family and domain databases | |
| Gene3D | 3.40.1350.10. 1 hit. |
| InterPro | IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. [Graphical view] |
| Pfam | PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view] |
| SUPFAM | SSF53032. tRNA_int_endo_C. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | SEN21_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9M1E8 Secondary accession number(s): Q9S7L3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |