Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9M1E8 (SEN21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit Sen2-1

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease Sen2-1
Short name=AtSen1
Gene names
Name:SEN1
Ordered Locus Names:At3g45590
ORF Names:F9K21.170
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
   Biological processmRNA processing
tRNA processing
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237tRNA-splicing endonuclease subunit Sen2-1
PRO_0000109456

Sites

Active site1481 By similarity
Active site1561 By similarity
Active site1901 By similarity

Natural variations

Natural variant241S → L in strain: cv. Landsberg erecta. Ref.1
Natural variant271R → Q in strain: cv. Landsberg erecta. Ref.1
Natural variant521E → Q in strain: cv. Landsberg erecta. Ref.1
Natural variant671G → S in strain: cv. Landsberg erecta. Ref.1
Natural variant1671G → S in strain: cv. Landsberg erecta. Ref.1
Natural variant1911S → T in strain: cv. Landsberg erecta. Ref.1
Natural variant199 – 2057RKVNTEK → GQVKTEN in strain: cv. Landsberg erecta.
Natural variant213 – 2142ED → DE in strain: cv. Landsberg erecta.

Sequences

Sequence LengthMass (Da)Tools
Q9M1E8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 2844856CB58CD0B2

FASTA23727,359
        10         20         30         40         50         60 
MAPRWKWKGA EAKALAEPVS KTVSELRSSL TQTEALGFLS SCNVLLSVES EEAELLDRCC 

        70         80         90        100        110        120 
FGRLVVGAEK DKRWIQLSFE EAFFLFYKLK CIKICLHGRS LENEVDLWRS MSSFKQDFAI 

       130        140        150        160        170        180 
LYKAYSHLRS KNWIVRSGLQ YGVDFVVYRH HPSLVHSEYA VLVQSIGGND RLKVWSDIHC 

       190        200        210        220        230 
SVRLTGSVAK SLLVLYVNRK VNTEKMNLPL CLEDYTVEEQ TIRRWSPELS REDETRT 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
Akama K., Junker V., Beier H.
Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS LEU-24; GLN-27; GLN-52; SER-67; SER-167; THR-191; 199-GLY--ASN-205 AND 213-ASP-GLU-214.
Strain: cv. Columbia and cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB036339 mRNA. Translation: BAA88627.1.
AB036340 Genomic DNA. Translation: BAA88628.1.
AL138657 Genomic DNA. Translation: CAB75488.1.
CP002686 Genomic DNA. Translation: AEE78047.1.
CP002686 Genomic DNA. Translation: AEE78048.1.
AK221492 mRNA. Translation: BAD94688.1.
PIRT47499.
RefSeqNP_001078245.1. NM_001084776.1.
NP_190145.1. NM_114428.3.
UniGeneAt.22349.
At.70243.

3D structure databases

ProteinModelPortalQ9M1E8.
SMRQ9M1E8. Positions 51-198.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G45590.1; AT3G45590.1; AT3G45590.
AT3G45590.2; AT3G45590.2; AT3G45590.
GeneID823701.
KEGGath:AT3G45590.

Organism-specific databases

GeneFarm5164. 493.
TAIRAT3G45590.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000030044.
InParanoidQ9M1E8.
KOK15322.
OMALRSKNWI.
PhylomeDBQ9M1E8.

Enzyme and pathway databases

BioCycARA:AT3G45590-MONOMER.
ARA:GQT-2187-MONOMER.

Gene expression databases

ArrayExpressQ9M1E8.
GenevestigatorQ9M1E8.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSEN21_ARATH
AccessionPrimary (citable) accession number: Q9M1E8
Secondary accession number(s): Q9S7L3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names