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Protein

Citrate synthase 5, mitochondrial

Gene

CSY5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase 5, mitochondrial (CSY5), Citrate synthase 1, peroxisomal (CSY1), Citrate synthase 2, peroxisomal (CSY2), Citrate synthase 3, peroxisomal (CSY3), Citrate synthase 4, mitochondrial (CSY4)
  2. Aconitate hydratase 3, mitochondrial (ACO3), Aconitate hydratase 1 (ACO1), Aconitate hydratase 2, mitochondrial (ACO2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei300PROSITE-ProRule annotation1
Active sitei346PROSITE-ProRule annotation1
Active sitei401PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-ATH-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 5, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:CSY5
Ordered Locus Names:At3g60100
ORF Names:T2O9.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G60100
TAIRilocus:2101467 AT3G60100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionSequence analysisAdd BLAST25
ChainiPRO_000000548526 – 464Citrate synthase 5, mitochondrialAdd BLAST439

Proteomic databases

PaxDbiQ9M1D3
PRIDEiQ9M1D3

Expressioni

Gene expression databases

ExpressionAtlasiQ9M1D3 differential
GenevisibleiQ9M1D3 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G60100.1

Structurei

3D structure databases

ProteinModelPortaliQ9M1D3
SMRiQ9M1D3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
HOGENOMiHOG000130831
InParanoidiQ9M1D3
KOiK01647
OMAiNSVRWLH
OrthoDBiEOG0936083W

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M1D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFFRSVSAI SRLRSRAVQQ SSLSNSVRWL HSSELDLKSQ MQEIIPEQQD
60 70 80 90 100
RLKKLKSEQG KVPVGNITVD MVLGGMRGMT GLLWETSLLD ADEGIRFRGM
110 120 130 140 150
SIPECQKILP SAESGEEPLP ESLLWLLLTG KVPTKEQANA LSTELAHRAA
160 170 180 190 200
VPAIDALPST AHPMTQFASG VMALQVQSEF QKAYEQGDIS KSKYWEPTFE
210 220 230 240 250
DALNLIARVP VVASYVYRRM YKDGSIIPLD DSLDYGANFS HMLGFDSPQM
260 270 280 290 300
KELMRLYVTI HSDHEGGNVS AHAGHLVGSA LSDPYLSFAA ALNGLAGPLH
310 320 330 340 350
GLANQEVLLW IKLVVEECGE SISKEQLKDY VWKTLNSGKV VPGYGHGVLR
360 370 380 390 400
KTDPRYICQR EFALKHLPDD PLFQLVSKLY EVVPPILTEL GKVKNPWPNV
410 420 430 440 450
DAHSGVLLNY YGLTEARYYT VLFGVSRSLG ICSQLIWDRA LGLPLERPKS
460
VNMDWLDNFT RLNR
Length:464
Mass (Da):51,724
Last modified:February 22, 2012 - v2
Checksum:i2D2AE88A6AA29BB2
GO

Sequence cautioni

The sequence CAB75925 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138658 Genomic DNA Translation: CAB75925.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE80011.1
PIRiT47834
RefSeqiNP_191569.2, NM_115873.3
UniGeneiAt.70776

Genome annotation databases

EnsemblPlantsiAT3G60100.1; AT3G60100.1; AT3G60100
GeneIDi825180
GrameneiAT3G60100.1; AT3G60100.1; AT3G60100
KEGGiath:AT3G60100

Similar proteinsi

Entry informationi

Entry nameiCISY5_ARATH
AccessioniPrimary (citable) accession number: Q9M1D3
Secondary accession number(s): F4JAL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 22, 2012
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health