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Protein

Beta-glucosidase 16

Gene

BGLU16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49SubstrateBy similarity1
Binding sitei153SubstrateBy similarity1
Binding sitei198SubstrateBy similarity1
Active sitei199Proton donorBy similarity1
Binding sitei343SubstrateBy similarity1
Active sitei413NucleophileBy similarity1
Binding sitei458SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G60130-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 16 (EC:3.2.1.21)
Short name:
AtBGLU16
Alternative name(s):
Protein YELLOW-LEAF-SPECIFIC GENE 1
Gene namesi
Name:BGLU16
Synonyms:YLS1
Ordered Locus Names:At3g60130
ORF Names:T2O9.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G60130.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000038957922 – 514Beta-glucosidase 16Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi218 ↔ 226By similarity
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9M1D0.

Expressioni

Tissue specificityi

Expressed at low levels in cauline leaves and flowers.1 Publication

Developmental stagei

Up-regulated in leaves during natural senescence.1 Publication

Inductioni

By abscisic acid (ABA) and dark.1 Publication

Gene expression databases

ExpressionAtlasiQ9M1D0. baseline and differential.
GenevisibleiQ9M1D0. AT.

Interactioni

Protein-protein interaction databases

BioGridi10497. 1 interactor.
IntActiQ9M1D0. 1 interactor.
STRINGi3702.AT3G60130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M1D0.
SMRiQ9M1D0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni465 – 466Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ9M1D0.
KOiK01188.
OMAiTRREYLM.
OrthoDBiEOG093606W7.
PhylomeDBiQ9M1D0.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9M1D0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGKFLSLLL LITLACIGVS AKKHSTRPRL RRNDFPQDFV FGSATSAYQC
60 70 80 90 100
EGAAHEDGRG PSIWDSFSEK FPEKIMDGSN GSIADDSYNL YKEDVNLLHQ
110 120 130 140 150
IGFDAYRFSI SWSRILPRGT LKGGINQAGI EYYNNLINQL ISKGVKPFVT
160 170 180 190 200
LFHWDLPDAL ENAYGGLLGD EFVNDFRDYA ELCFQKFGDR VKQWTTLNEP
210 220 230 240 250
YTMVHEGYIT GQKAPGRCSN FYKPDCLGGD AATEPYIVGH NLLLAHGVAV
260 270 280 290 300
KVYREKYQAT QKGEIGIALN TAWHYPYSDS YADRLAATRA TAFTFDYFME
310 320 330 340 350
PIVYGRYPIE MVSHVKDGRL PTFTPEESEM LKGSYDFIGV NYYSSLYAKD
360 370 380 390 400
VPCATENITM TTDSCVSLVG ERNGVPIGPA AGSDWLLIYP KGIRDLLLHA
410 420 430 440 450
KFRYNDPVLY ITENGVDEAN IGKIFLNDDL RIDYYAHHLK MVSDAISIGV
460 470 480 490 500
NVKGYFAWSL MDNFEWSEGY TVRFGLVFVD FEDGRKRYLK KSAKWFRRLL
510
KGAHGGTNEQ VAVI
Length:514
Mass (Da):58,043
Last modified:October 1, 2000 - v1
Checksum:iED42E2FC638FDB6D
GO
Isoform 2 (identifier: Q9M1D0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-391: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:503
Mass (Da):56,798
Checksum:iC7042262947B460A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038455381 – 391Missing in isoform 2. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138658 Genomic DNA. Translation: CAB75928.1.
CP002686 Genomic DNA. Translation: AEE80014.1.
AY045953 mRNA. Translation: AAK76627.1.
AY113935 mRNA. Translation: AAM44983.1.
AB047804 mRNA. Translation: BAB32881.1.
PIRiT47837.
RefSeqiNP_191572.1. NM_115876.5. [Q9M1D0-1]
UniGeneiAt.24631.

Genome annotation databases

EnsemblPlantsiAT3G60130.1; AT3G60130.1; AT3G60130. [Q9M1D0-1]
GeneIDi825183.
GrameneiAT3G60130.1; AT3G60130.1; AT3G60130.
KEGGiath:AT3G60130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138658 Genomic DNA. Translation: CAB75928.1.
CP002686 Genomic DNA. Translation: AEE80014.1.
AY045953 mRNA. Translation: AAK76627.1.
AY113935 mRNA. Translation: AAM44983.1.
AB047804 mRNA. Translation: BAB32881.1.
PIRiT47837.
RefSeqiNP_191572.1. NM_115876.5. [Q9M1D0-1]
UniGeneiAt.24631.

3D structure databases

ProteinModelPortaliQ9M1D0.
SMRiQ9M1D0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10497. 1 interactor.
IntActiQ9M1D0. 1 interactor.
STRINGi3702.AT3G60130.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ9M1D0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G60130.1; AT3G60130.1; AT3G60130. [Q9M1D0-1]
GeneIDi825183.
GrameneiAT3G60130.1; AT3G60130.1; AT3G60130.
KEGGiath:AT3G60130.

Organism-specific databases

TAIRiAT3G60130.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ9M1D0.
KOiK01188.
OMAiTRREYLM.
OrthoDBiEOG093606W7.
PhylomeDBiQ9M1D0.

Enzyme and pathway databases

BioCyciARA:AT3G60130-MONOMER.

Miscellaneous databases

PROiQ9M1D0.

Gene expression databases

ExpressionAtlasiQ9M1D0. baseline and differential.
GenevisibleiQ9M1D0. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL16_ARATH
AccessioniPrimary (citable) accession number: Q9M1D0
Secondary accession number(s): Q9CAZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.