Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9M1D0 (BGL16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 16

Short name=AtBGLU16
EC=3.2.1.21
Alternative name(s):
Protein YELLOW-LEAF-SPECIFIC GENE 1
Gene names
Name:BGLU16
Synonyms:YLS1
Ordered Locus Names:At3g60130
ORF Names:T2O9.110
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Tissue specificity

Expressed at low levels in cauline leaves and flowers. Ref.4

Developmental stage

Up-regulated in leaves during natural senescence. Ref.4

Induction

By abscisic acid (ABA) and dark. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9M1D0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9M1D0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     381-391: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 514493Beta-glucosidase 16
PRO_0000389579

Regions

Region465 – 4662Substrate binding By similarity

Sites

Active site1991Proton donor By similarity
Active site4131Nucleophile By similarity
Binding site491Substrate By similarity
Binding site1531Substrate By similarity
Binding site1981Substrate By similarity
Binding site3431Substrate By similarity
Binding site4581Substrate By similarity

Amino acid modifications

Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation3571N-linked (GlcNAc...) Potential
Disulfide bond218 ↔ 226 By similarity

Natural variations

Alternative sequence381 – 39111Missing in isoform 2.
VSP_038455

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: ED42E2FC638FDB6D

FASTA51458,043
        10         20         30         40         50         60 
MRGKFLSLLL LITLACIGVS AKKHSTRPRL RRNDFPQDFV FGSATSAYQC EGAAHEDGRG 

        70         80         90        100        110        120 
PSIWDSFSEK FPEKIMDGSN GSIADDSYNL YKEDVNLLHQ IGFDAYRFSI SWSRILPRGT 

       130        140        150        160        170        180 
LKGGINQAGI EYYNNLINQL ISKGVKPFVT LFHWDLPDAL ENAYGGLLGD EFVNDFRDYA 

       190        200        210        220        230        240 
ELCFQKFGDR VKQWTTLNEP YTMVHEGYIT GQKAPGRCSN FYKPDCLGGD AATEPYIVGH 

       250        260        270        280        290        300 
NLLLAHGVAV KVYREKYQAT QKGEIGIALN TAWHYPYSDS YADRLAATRA TAFTFDYFME 

       310        320        330        340        350        360 
PIVYGRYPIE MVSHVKDGRL PTFTPEESEM LKGSYDFIGV NYYSSLYAKD VPCATENITM 

       370        380        390        400        410        420 
TTDSCVSLVG ERNGVPIGPA AGSDWLLIYP KGIRDLLLHA KFRYNDPVLY ITENGVDEAN 

       430        440        450        460        470        480 
IGKIFLNDDL RIDYYAHHLK MVSDAISIGV NVKGYFAWSL MDNFEWSEGY TVRFGLVFVD 

       490        500        510 
FEDGRKRYLK KSAKWFRRLL KGAHGGTNEQ VAVI 

« Hide

Isoform 2 [UniParc].

Checksum: C7042262947B460A
Show »

FASTA50356,798

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana."
Yoshida S., Ito M., Nishida I., Watanabe A.
Plant Cell Physiol. 42:170-178(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-510 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Tissue: Leaf.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL138658 Genomic DNA. Translation: CAB75928.1.
CP002686 Genomic DNA. Translation: AEE80014.1.
AY045953 mRNA. Translation: AAK76627.1.
AY113935 mRNA. Translation: AAM44983.1.
AB047804 mRNA. Translation: BAB32881.1.
PIRT47837.
RefSeqNP_191572.1. NM_115876.4. [Q9M1D0-1]
UniGeneAt.24631.

3D structure databases

ProteinModelPortalQ9M1D0.
SMRQ9M1D0. Positions 30-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10497. 1 interaction.
IntActQ9M1D0. 1 interaction.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ9M1D0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G60130.1; AT3G60130.1; AT3G60130. [Q9M1D0-1]
GeneID825183.
KEGGath:AT3G60130.

Organism-specific databases

TAIRAT3G60130.

Phylogenomic databases

HOGENOMHOG000088630.
InParanoidQ9M1D0.
KOK01188.
OMACATENIT.
PhylomeDBQ9M1D0.

Enzyme and pathway databases

BioCycARA:AT3G60130-MONOMER.
ARA:GQT-68-MONOMER.
ARA:GQT-69-MONOMER.

Gene expression databases

ArrayExpressQ9M1D0.
GenevestigatorQ9M1D0.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL16_ARATH
AccessionPrimary (citable) accession number: Q9M1D0
Secondary accession number(s): Q9CAZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names