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Q9M1D0

- BGL16_ARATH

UniProt

Q9M1D0 - BGL16_ARATH

Protein

Beta-glucosidase 16

Gene

BGLU16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491SubstrateBy similarity
    Binding sitei153 – 1531SubstrateBy similarity
    Binding sitei198 – 1981SubstrateBy similarity
    Active sitei199 – 1991Proton donorBy similarity
    Binding sitei343 – 3431SubstrateBy similarity
    Active sitei413 – 4131NucleophileBy similarity
    Binding sitei458 – 4581SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT3G60130-MONOMER.
    ARA:GQT-68-MONOMER.
    ARA:GQT-69-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 16 (EC:3.2.1.21)
    Short name:
    AtBGLU16
    Alternative name(s):
    Protein YELLOW-LEAF-SPECIFIC GENE 1
    Gene namesi
    Name:BGLU16
    Synonyms:YLS1
    Ordered Locus Names:At3g60130
    ORF Names:T2O9.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G60130.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 514493Beta-glucosidase 16PRO_0000389579Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi218 ↔ 226By similarity
    Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9M1D0.

    Expressioni

    Tissue specificityi

    Expressed at low levels in cauline leaves and flowers.1 Publication

    Developmental stagei

    Up-regulated in leaves during natural senescence.1 Publication

    Inductioni

    By abscisic acid (ABA) and dark.1 Publication

    Gene expression databases

    ArrayExpressiQ9M1D0.
    GenevestigatoriQ9M1D0.

    Interactioni

    Protein-protein interaction databases

    BioGridi10497. 1 interaction.
    IntActiQ9M1D0. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M1D0.
    SMRiQ9M1D0. Positions 30-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni465 – 4662Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000088630.
    InParanoidiQ9M1D0.
    KOiK01188.
    OMAiCATENIT.
    PhylomeDBiQ9M1D0.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9M1D0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRGKFLSLLL LITLACIGVS AKKHSTRPRL RRNDFPQDFV FGSATSAYQC    50
    EGAAHEDGRG PSIWDSFSEK FPEKIMDGSN GSIADDSYNL YKEDVNLLHQ 100
    IGFDAYRFSI SWSRILPRGT LKGGINQAGI EYYNNLINQL ISKGVKPFVT 150
    LFHWDLPDAL ENAYGGLLGD EFVNDFRDYA ELCFQKFGDR VKQWTTLNEP 200
    YTMVHEGYIT GQKAPGRCSN FYKPDCLGGD AATEPYIVGH NLLLAHGVAV 250
    KVYREKYQAT QKGEIGIALN TAWHYPYSDS YADRLAATRA TAFTFDYFME 300
    PIVYGRYPIE MVSHVKDGRL PTFTPEESEM LKGSYDFIGV NYYSSLYAKD 350
    VPCATENITM TTDSCVSLVG ERNGVPIGPA AGSDWLLIYP KGIRDLLLHA 400
    KFRYNDPVLY ITENGVDEAN IGKIFLNDDL RIDYYAHHLK MVSDAISIGV 450
    NVKGYFAWSL MDNFEWSEGY TVRFGLVFVD FEDGRKRYLK KSAKWFRRLL 500
    KGAHGGTNEQ VAVI 514
    Length:514
    Mass (Da):58,043
    Last modified:October 1, 2000 - v1
    Checksum:iED42E2FC638FDB6D
    GO
    Isoform 2 (identifier: Q9M1D0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         381-391: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:503
    Mass (Da):56,798
    Checksum:iC7042262947B460A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei381 – 39111Missing in isoform 2. CuratedVSP_038455Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138658 Genomic DNA. Translation: CAB75928.1.
    CP002686 Genomic DNA. Translation: AEE80014.1.
    AY045953 mRNA. Translation: AAK76627.1.
    AY113935 mRNA. Translation: AAM44983.1.
    AB047804 mRNA. Translation: BAB32881.1.
    PIRiT47837.
    RefSeqiNP_191572.1. NM_115876.4. [Q9M1D0-1]
    UniGeneiAt.24631.

    Genome annotation databases

    EnsemblPlantsiAT3G60130.1; AT3G60130.1; AT3G60130. [Q9M1D0-1]
    GeneIDi825183.
    KEGGiath:AT3G60130.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138658 Genomic DNA. Translation: CAB75928.1 .
    CP002686 Genomic DNA. Translation: AEE80014.1 .
    AY045953 mRNA. Translation: AAK76627.1 .
    AY113935 mRNA. Translation: AAM44983.1 .
    AB047804 mRNA. Translation: BAB32881.1 .
    PIRi T47837.
    RefSeqi NP_191572.1. NM_115876.4. [Q9M1D0-1 ]
    UniGenei At.24631.

    3D structure databases

    ProteinModelPortali Q9M1D0.
    SMRi Q9M1D0. Positions 30-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 10497. 1 interaction.
    IntActi Q9M1D0. 1 interaction.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q9M1D0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G60130.1 ; AT3G60130.1 ; AT3G60130 . [Q9M1D0-1 ]
    GeneIDi 825183.
    KEGGi ath:AT3G60130.

    Organism-specific databases

    TAIRi AT3G60130.

    Phylogenomic databases

    HOGENOMi HOG000088630.
    InParanoidi Q9M1D0.
    KOi K01188.
    OMAi CATENIT.
    PhylomeDBi Q9M1D0.

    Enzyme and pathway databases

    BioCyci ARA:AT3G60130-MONOMER.
    ARA:GQT-68-MONOMER.
    ARA:GQT-69-MONOMER.

    Gene expression databases

    ArrayExpressi Q9M1D0.
    Genevestigatori Q9M1D0.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana."
      Yoshida S., Ito M., Nishida I., Watanabe A.
      Plant Cell Physiol. 42:170-178(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 315-510 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Tissue: Leaf.
    5. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL16_ARATH
    AccessioniPrimary (citable) accession number: Q9M1D0
    Secondary accession number(s): Q9CAZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3