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Protein

UDP-glycosyltransferase 72B1

Gene

UGT72B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional O-glycosyltransferase and N-glycosyltransferase that can detoxify xenobiotics. Possesses high activity to metabolize the peristent pollutants 2,4,5-trichlorophenol (TCP) and 3,4-dichloroaniline (DCA). Also active on benzoates and benzoate derivatives in vitro.3 Publications

Catalytic activityi

UDP-glucose + hydroquinone = UDP + hydroquinone-O-beta-D-glucopyranoside.

Kineticsi

  1. KM=34.3 µM for 3,4-dichlorophenol1 Publication
  2. KM=16.5 µM for 3,4-dichloroaniline1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei277UDP-glucoseCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • flavonoid biosynthetic process Source: GO_Central
    • flavonoid glucuronidation Source: GO_Central
    • lignin biosynthetic process Source: TAIR
    • response to salt stress Source: TAIR
    • response to toxic substance Source: TAIR
    • xenobiotic catabolic process Source: TAIR
    • xenobiotic metabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Detoxification

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glycosyltransferase 72B1 (EC:2.4.1.-)
    Alternative name(s):
    Arbutin synthase
    Probable hydroquinone glucosyltransferase (EC:2.4.1.218)
    Gene namesi
    Name:UGT72B1
    Ordered Locus Names:At4g01070
    ORF Names:F2N1.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G01070.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi14S → A: Loss of activity. 1 Publication1
    Mutagenesisi19H → A: Loss of activity. 1 Publication1
    Mutagenesisi19H → Q: Reduces N-glycosyltransferase activity. Loss of O-glycosyltransferase activity. 1 Publication1
    Mutagenesisi117D → A: Loss of activity. 1 Publication1
    Mutagenesisi312N → D: Decreases activity. Loss of N-glycosyltransferase activity; when associated with F-315. 1 Publication1
    Mutagenesisi315Y → F: Decreases activity. Loss of N-glycosyltransferase activity; when associated with D-312. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000741601 – 480UDP-glycosyltransferase 72B1Add BLAST480

    Proteomic databases

    PaxDbiQ9M156.
    PRIDEiQ9M156.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ9M156. baseline and differential.
    GenevisibleiQ9M156. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi13203. 1 interactor.
    STRINGi3702.AT4G01070.1.

    Structurei

    Secondary structure

    1480
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi8 – 12Combined sources5
    Helixi17 – 34Combined sources18
    Beta strandi37 – 42Combined sources6
    Beta strandi45 – 47Combined sources3
    Helixi52 – 56Combined sources5
    Beta strandi63 – 67Combined sources5
    Helixi82 – 91Combined sources10
    Helixi94 – 106Combined sources13
    Beta strandi112 – 116Combined sources5
    Helixi121 – 123Combined sources3
    Helixi124 – 129Combined sources6
    Beta strandi134 – 138Combined sources5
    Helixi142 – 157Combined sources16
    Helixi162 – 164Combined sources3
    Helixi179 – 181Combined sources3
    Helixi184 – 186Combined sources3
    Helixi192 – 203Combined sources12
    Helixi204 – 206Combined sources3
    Beta strandi208 – 213Combined sources6
    Turni216 – 218Combined sources3
    Helixi220 – 227Combined sources8
    Beta strandi236 – 238Combined sources3
    Helixi257 – 263Combined sources7
    Beta strandi270 – 274Combined sources5
    Turni276 – 278Combined sources3
    Helixi283 – 295Combined sources13
    Beta strandi299 – 304Combined sources6
    Turni311 – 316Combined sources6
    Helixi324 – 327Combined sources4
    Helixi332 – 335Combined sources4
    Turni336 – 339Combined sources4
    Beta strandi340 – 345Combined sources6
    Helixi349 – 354Combined sources6
    Beta strandi358 – 363Combined sources6
    Helixi367 – 376Combined sources10
    Beta strandi380 – 382Combined sources3
    Helixi389 – 398Combined sources10
    Helixi416 – 427Combined sources12
    Helixi431 – 449Combined sources19
    Helixi455 – 475Combined sources21

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2VCEX-ray1.90A1-480[»]
    2VCHX-ray1.45A1-480[»]
    2VG8X-ray1.75A1-480[»]
    ProteinModelPortaliQ9M156.
    SMRiQ9M156.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9M156.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni346 – 347UDP-glucose bindingCombined sources1 Publication2
    Regioni364 – 372UDP-glucose bindingCombined sources1 Publication9
    Regioni386 – 389UDP-glucose bindingCombined sources1 Publication4

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237568.
    InParanoidiQ9M156.
    KOiK08237.
    OMAiHGARYRE.
    OrthoDBiEOG09360AZC.
    PhylomeDBiQ9M156.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9M156-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEESKTPHVA IIPSPGMGHL IPLVEFAKRL VHLHGLTVTF VIAGEGPPSK
    60 70 80 90 100
    AQRTVLDSLP SSISSVFLPP VDLTDLSSST RIESRISLTV TRSNPELRKV
    110 120 130 140 150
    FDSFVEGGRL PTALVVDLFG TDAFDVAVEF HVPPYIFYPT TANVLSFFLH
    160 170 180 190 200
    LPKLDETVSC EFRELTEPLM LPGCVPVAGK DFLDPAQDRK DDAYKWLLHN
    210 220 230 240 250
    TKRYKEAEGI LVNTFFELEP NAIKALQEPG LDKPPVYPVG PLVNIGKQEA
    260 270 280 290 300
    KQTEESECLK WLDNQPLGSV LYVSFGSGGT LTCEQLNELA LGLADSEQRF
    310 320 330 340 350
    LWVIRSPSGI ANSSYFDSHS QTDPLTFLPP GFLERTKKRG FVIPFWAPQA
    360 370 380 390 400
    QVLAHPSTGG FLTHCGWNST LESVVSGIPL IAWPLYAEQK MNAVLLSEDI
    410 420 430 440 450
    RAALRPRAGD DGLVRREEVA RVVKGLMEGE EGKGVRNKMK ELKEAACRVL
    460 470 480
    KDDGTSTKAL SLVALKWKAH KKELEQNGNH
    Length:480
    Mass (Da):52,930
    Last modified:October 1, 2000 - v1
    Checksum:i3E0315C1D71D2DB0
    GO

    Sequence cautioni

    The sequence AAB61023 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF007269 Genomic DNA. Translation: AAB61023.1. Sequence problems.
    AL161491 Genomic DNA. Translation: CAB80916.1.
    CP002687 Genomic DNA. Translation: AEE81977.1.
    AF360262 mRNA. Translation: AAK25972.1.
    AY040075 mRNA. Translation: AAK64133.1.
    AY084892 mRNA. Translation: AAM61455.1.
    PIRiB85014.
    T01732.
    RefSeqiNP_192016.1. NM_116337.3. [Q9M156-1]
    UniGeneiAt.22609.

    Genome annotation databases

    EnsemblPlantsiAT4G01070.1; AT4G01070.1; AT4G01070. [Q9M156-1]
    GeneIDi827912.
    GrameneiAT4G01070.1; AT4G01070.1; AT4G01070.
    KEGGiath:AT4G01070.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF007269 Genomic DNA. Translation: AAB61023.1. Sequence problems.
    AL161491 Genomic DNA. Translation: CAB80916.1.
    CP002687 Genomic DNA. Translation: AEE81977.1.
    AF360262 mRNA. Translation: AAK25972.1.
    AY040075 mRNA. Translation: AAK64133.1.
    AY084892 mRNA. Translation: AAM61455.1.
    PIRiB85014.
    T01732.
    RefSeqiNP_192016.1. NM_116337.3. [Q9M156-1]
    UniGeneiAt.22609.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2VCEX-ray1.90A1-480[»]
    2VCHX-ray1.45A1-480[»]
    2VG8X-ray1.75A1-480[»]
    ProteinModelPortaliQ9M156.
    SMRiQ9M156.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi13203. 1 interactor.
    STRINGi3702.AT4G01070.1.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Proteomic databases

    PaxDbiQ9M156.
    PRIDEiQ9M156.

    Protocols and materials databases

    DNASUi827912.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G01070.1; AT4G01070.1; AT4G01070. [Q9M156-1]
    GeneIDi827912.
    GrameneiAT4G01070.1; AT4G01070.1; AT4G01070.
    KEGGiath:AT4G01070.

    Organism-specific databases

    TAIRiAT4G01070.

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237568.
    InParanoidiQ9M156.
    KOiK08237.
    OMAiHGARYRE.
    OrthoDBiEOG09360AZC.
    PhylomeDBiQ9M156.

    Miscellaneous databases

    EvolutionaryTraceiQ9M156.
    PROiQ9M156.

    Gene expression databases

    ExpressionAtlasiQ9M156. baseline and differential.
    GenevisibleiQ9M156. AT.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiU72B1_ARATH
    AccessioniPrimary (citable) accession number: Q9M156
    Secondary accession number(s): O04622
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 12, 2003
    Last sequence update: October 1, 2000
    Last modified: November 30, 2016
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.