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Protein

UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase MGP4

Gene

MGP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of D-xylose from UDP-alpha-D-xylose onto L-fucose. Probably involved in the biosynthesis of rhamnogalacturonan II (RG-II) through xylosylation of the internal fucose moiety of the A-chain of RG-II, a structurally complex pectic polysaccharide of the primary cell wall. RG-II is essential for the cell wall integrity of rapidly growing tissues such as roots and pollen tube growth and elongation.1 Publication

Cofactori

Mn2+By similarity, Mg2+By similarity

GO - Molecular functioni

  • UDP-xylosyltransferase activity Source: GO_Central
  • xylosyltransferase activity Source: TAIR

GO - Biological processi

  • pollen tube development Source: TAIR
  • rhamnogalacturonan II biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGT77. Glycosyltransferase Family 77.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase MGP4Curated (EC:2.4.2.-Curated)
Alternative name(s):
Protein MALE GAMETOPHYTE DEFECTIVE 41 Publication
Rhamnogalacturonan xylosyltransferase MGP4Curated
Gene namesi
Name:MGP41 Publication
Ordered Locus Names:At4g01220
ORF Names:F2N1.35
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G01220.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence analysisAdd
BLAST
Transmembranei42 – 6221Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini63 – 360298LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced fertility due to impaired growth of pollen tubes in the transmitting tract during fertilization.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase MGP4PRO_0000423716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence analysis
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence analysis
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M146.
PRIDEiQ9M146.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

ExpressionAtlasiQ9M146. baseline and differential.
GenevisibleiQ9M146. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G01220.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933DXD motifCurated

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 77 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFDF. Eukaryota.
ENOG4111Z77. LUCA.
HOGENOMiHOG000241399.
InParanoidiQ9M146.
KOiK11714.
OMAiKRFRDYG.
PhylomeDBiQ9M146.

Family and domain databases

InterProiIPR005069. Nucl-diP-sugar_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF03407. Nucleotid_trans. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9M146-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQKFLHQR PIQNPFTNPF SSSPLSTSSI SNRPISLLSR NGLLLLLALL
60 70 80 90 100
VILGVFLPWA GSPLFPSPNK LSPSQSKWRD YSLPQAVKFV AKNGTVIVCA
110 120 130 140 150
VSYPYLPFLN NWLISVSRQK HQDQVLVIAE DYATLYKVNE KWPGHAVLIP
160 170 180 190 200
PALDSQTAHK FGSQGFFNFT ARRPQHLLEI LELGYNVMYN DVDMVWLQDP
210 220 230 240 250
FQYLEGKHDA YFMDDMTAIK PLDHSHDLPP PGKKGRTYIC SCMIFLRPTN
260 270 280 290 300
GAKLLMKKWI EELETQPWSR AKKANDQPGF NWALNKTANQ VDMYLLSQAA
310 320 330 340 350
FPTGGLYFKN KTWVKETKGK HAIIHNNYIV GFEKKIKRFR DFNLWLVDDH
360
ASESPLGKLE
Length:360
Mass (Da):41,121
Last modified:October 1, 2000 - v1
Checksum:iA7E4CBAE9369D931
GO
Isoform 2 (identifier: Q9M146-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-299: DMYLLSQA → CSFFLVPQ
     300-360: Missing.

Note: No experimental confirmation available.
Show »
Length:299
Mass (Da):34,004
Checksum:i2FF9FD0898E3C82C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711L → H in AAK43874 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei292 – 2998DMYLLSQA → CSFFLVPQ in isoform 2. 2 PublicationsVSP_053260
Alternative sequencei300 – 36061Missing in isoform 2. 2 PublicationsVSP_053261Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161491 Genomic DNA. Translation: CAB80931.1.
CP002687 Genomic DNA. Translation: AEE81995.1.
AF370497 mRNA. Translation: AAK43874.1.
CP002687 Genomic DNA. Translation: AEE81996.1.
BT020481 mRNA. Translation: AAW38982.1.
BT033130 mRNA. Translation: ACF28391.1.
PIRiA85016.
RefSeqiNP_567211.1. NM_116352.2. [Q9M146-2]
NP_849279.1. NM_178948.2. [Q9M146-1]
UniGeneiAt.20197.

Genome annotation databases

EnsemblPlantsiAT4G01220.1; AT4G01220.1; AT4G01220. [Q9M146-1]
AT4G01220.2; AT4G01220.2; AT4G01220. [Q9M146-2]
GeneIDi828028.
KEGGiath:AT4G01220.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161491 Genomic DNA. Translation: CAB80931.1.
CP002687 Genomic DNA. Translation: AEE81995.1.
AF370497 mRNA. Translation: AAK43874.1.
CP002687 Genomic DNA. Translation: AEE81996.1.
BT020481 mRNA. Translation: AAW38982.1.
BT033130 mRNA. Translation: ACF28391.1.
PIRiA85016.
RefSeqiNP_567211.1. NM_116352.2. [Q9M146-2]
NP_849279.1. NM_178948.2. [Q9M146-1]
UniGeneiAt.20197.

3D structure databases

ProteinModelPortaliQ9M146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G01220.1.

Protein family/group databases

CAZyiGT77. Glycosyltransferase Family 77.

Proteomic databases

PaxDbiQ9M146.
PRIDEiQ9M146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G01220.1; AT4G01220.1; AT4G01220. [Q9M146-1]
AT4G01220.2; AT4G01220.2; AT4G01220. [Q9M146-2]
GeneIDi828028.
KEGGiath:AT4G01220.

Organism-specific databases

TAIRiAT4G01220.

Phylogenomic databases

eggNOGiENOG410IFDF. Eukaryota.
ENOG4111Z77. LUCA.
HOGENOMiHOG000241399.
InParanoidiQ9M146.
KOiK11714.
OMAiKRFRDYG.
PhylomeDBiQ9M146.

Miscellaneous databases

PROiQ9M146.

Gene expression databases

ExpressionAtlasiQ9M146. baseline and differential.
GenevisibleiQ9M146. AT.

Family and domain databases

InterProiIPR005069. Nucl-diP-sugar_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF03407. Nucleotid_trans. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.
    Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Male gametophyte defective 4 encodes a rhamnogalacturonan II xylosyltransferase and is important for growth of pollen tubes and roots in Arabidopsis."
    Liu X.L., Liu L., Niu Q.K., Xia C., Yang K.Z., Li R., Chen L.Q., Zhang X.Q., Zhou Y., Ye D.
    Plant J. 65:647-660(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  7. "A putative Arabidopsis thaliana glycosyltransferase, At4g01220, which is closely related to three plant cell wall-specific xylosyltransferases, is differentially expressed spatially and temporally."
    Fangel J.U., Petersen B.L., Jensen N.B., Willats W.G., Bacic A., Egelund J.
    Plant Sci. 180:470-479(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMGP4_ARATH
AccessioniPrimary (citable) accession number: Q9M146
Secondary accession number(s): Q5HZ43, Q94K03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.