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Protein

Sucrose synthase 3

Gene

SUS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Modulates metabolic homeostasis and direct carbon towards starch synthesis in developing seeds.1 Publication

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.

Kineticsi

  1. KM=41.96 mM for D-fructose (synthetic reaction) at pH 9.42 Publications
  2. KM=0.2 mM for UDP-glucose (synthetic reaction) at pH 9.42 Publications
  3. KM=108.2 mM for sucrose (degradative reaction) at pH 62 Publications
  4. KM=48 mM for sucrose (degradative reaction) at pH 72 Publications
  5. KM=0.09 mM for UDP (degradative reaction) at pH 62 Publications
  6. KM=0.25 mM for UDP (degradative reaction) at pH 72 Publications
  7. KM=0.15 mM for ADP (degradative reaction) at pH 72 Publications
  1. Vmax=7.32 µmol/min/mg enzyme for synthetic reaction at pH 9.42 Publications
  2. Vmax=3.03 µmol/min/mg enzyme for degradative reaction at pH 62 Publications
  3. Vmax=950 µmol/min/mg enzyme for degradative reaction at pH 72 Publications

pH dependencei

Optimum pH is 6.0-7.0 for degradative reaction and 7.0 (PubMed:22184213) or 9.0-9.5 (PubMed:17257168) for synthetic reaction.2 Publications

GO - Molecular functioni

  • sucrose synthase activity Source: UniProtKB

GO - Biological processi

  • biosynthetic process Source: InterPro
  • response to mannitol Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • seed maturation Source: TAIR
  • starch metabolic process Source: TAIR
  • sucrose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciMetaCyc:AT4G02280-MONOMER.
BRENDAi2.4.1.13. 399.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose synthase 3 (EC:2.4.1.13)
Short name:
AtSUS3
Alternative name(s):
Sucrose-UDP glucosyltransferase 3
Gene namesi
Name:SUS3
Ordered Locus Names:At4g02280
ORF Names:T2H3.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02280.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Diminution of the starch content of developing seeds and increased lipid accumulation early during seed development.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Sucrose synthase 3PRO_0000418802Add
BLAST

Proteomic databases

PRIDEiQ9M111.

Expressioni

Tissue specificityi

Detected in the whole plant with highest expression in developing siliques, vasculature of cotyledons and stomatal guard cells. Also detected throughout the mature parts of the root but not in the expanding zone.3 Publications

Developmental stagei

Highly expressed in late-maturing seeds and in geminating seeds (at the protein level).2 Publications

Inductioni

By drought stress. By mannitol, an osmotic agent. Positively regulated by LEC2.2 Publications

Gene expression databases

ExpressionAtlasiQ9M111. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi13372. 1 interaction.
STRINGi3702.AT4G02280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M111.
SMRiQ9M111. Positions 25-808.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 755479GT-B glycosyltransferaseBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000240125.
InParanoidiQ9M111.
KOiK00695.
OMAiFQRCKED.
PhylomeDBiQ9M111.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PANTHERiPTHR12526:SF27. PTHR12526:SF27. 1 hit.
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPKLTRVL STRDRVQDTL SAHRNELVAL LSRYVDQGKG ILQPHNLIDE
60 70 80 90 100
LESVIGDDET KKSLSDGPFG EILKSAMEAI VVPPFVALAV RPRPGVWEYV
110 120 130 140 150
RVNVFELSVE QLTVSEYLRF KEELVDGPNS DPFCLELDFE PFNANVPRPS
160 170 180 190 200
RSSSIGNGVQ FLNRHLSSVM FRNKDCLEPL LDFLRVHKYK GHPLMLNDRI
210 220 230 240 250
QSISRLQIQL SKAEDHISKL SQETPFSEFE YALQGMGFEK GWGDTAGRVL
260 270 280 290 300
EMMHLLSDIL QAPDPSSLEK FLGMVPMVFN VVILSPHGYF GQANVLGLPD
310 320 330 340 350
TGGQVVYILD QVRALETEML LRIKRQGLDI SPSILIVTRL IPDAKGTTCN
360 370 380 390 400
QRLERVSGTE HTHILRVPFR SEKGILRKWI SRFDVWPYLE NYAQDAASEI
410 420 430 440 450
VGELQGVPDF IIGNYSDGNL VASLMAHRMG VTQCTIAHAL EKTKYPDSDI
460 470 480 490 500
YWKDFDNKYH FSCQFTADLI AMNNADFIIT STYQEIAGTK NTVGQYESHG
510 520 530 540 550
AFTLPGLYRV VHGIDVFDPK FNIVSPGADM TIYFPYSEET RRLTALHGSI
560 570 580 590 600
EEMLYSPDQT DEHVGTLSDR SKPILFSMAR LDKVKNISGL VEMYSKNTKL
610 620 630 640 650
RELVNLVVIA GNIDVNKSKD REEIVEIEKM HNLMKNYKLD GQFRWITAQT
660 670 680 690 700
NRARNGELYR YIADTRGAFA QPAFYEAFGL TVVEAMTCGL PTFATCHGGP
710 720 730 740 750
AEIIEHGLSG FHIDPYHPEQ AGNIMADFFE RCKEDPNHWK KVSDAGLQRI
760 770 780 790 800
YERYTWKIYS ERLMTLAGVY GFWKYVSKLE RRETRRYLEM FYILKFRDLV

KTVPSTADD
Length:809
Mass (Da):92,002
Last modified:October 1, 2000 - v1
Checksum:iBC5151F2FBF265D5
GO

Sequence cautioni

The sequence AAC28175.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075597 Genomic DNA. Translation: AAC28175.1. Sequence problems.
AL161494 Genomic DNA. Translation: CAB80721.1.
CP002687 Genomic DNA. Translation: AEE82150.1.
AY051001 mRNA. Translation: AAK93678.1.
AY056784 mRNA. Translation: AAL09730.1.
AY142511 mRNA. Translation: AAN13112.1.
PIRiB85029.
T01420.
RefSeqiNP_192137.1. NM_116461.3.
UniGeneiAt.3877.

Genome annotation databases

EnsemblPlantsiAT4G02280.1; AT4G02280.1; AT4G02280.
GeneIDi828081.
KEGGiath:AT4G02280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075597 Genomic DNA. Translation: AAC28175.1. Sequence problems.
AL161494 Genomic DNA. Translation: CAB80721.1.
CP002687 Genomic DNA. Translation: AEE82150.1.
AY051001 mRNA. Translation: AAK93678.1.
AY056784 mRNA. Translation: AAL09730.1.
AY142511 mRNA. Translation: AAN13112.1.
PIRiB85029.
T01420.
RefSeqiNP_192137.1. NM_116461.3.
UniGeneiAt.3877.

3D structure databases

ProteinModelPortaliQ9M111.
SMRiQ9M111. Positions 25-808.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13372. 1 interaction.
STRINGi3702.AT4G02280.1.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEiQ9M111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02280.1; AT4G02280.1; AT4G02280.
GeneIDi828081.
KEGGiath:AT4G02280.

Organism-specific databases

TAIRiAT4G02280.

Phylogenomic databases

HOGENOMiHOG000240125.
InParanoidiQ9M111.
KOiK00695.
OMAiFQRCKED.
PhylomeDBiQ9M111.

Enzyme and pathway databases

BioCyciMetaCyc:AT4G02280-MONOMER.
BRENDAi2.4.1.13. 399.

Miscellaneous databases

PROiQ9M111.

Gene expression databases

ExpressionAtlasiQ9M111. baseline and differential.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PANTHERiPTHR12526:SF27. PTHR12526:SF27. 1 hit.
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Structure and expression profile of the sucrose synthase multigene family in Arabidopsis."
    Baud S., Vaultier M.N., Rochat C.
    J. Exp. Bot. 55:397-409(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  5. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. "Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development."
    Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.
    J. Exp. Bot. 59:3283-3295(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis and direct carbon towards starch synthesis in developing seeds."
    Angeles-Nunez J.G., Tiessen A.
    Planta 232:701-718(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Regulation of AtSUS2 and AtSUS3 by glucose and the transcription factor LEC2 in different tissues and at different stages of Arabidopsis seed development."
    Angeles-Nunez J.G., Tiessen A.
    Plant Mol. Biol. 78:377-392(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LEC2.
  9. "Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production."
    Baroja-Fernandez E., Munoz F.J., Li J., Bahaji A., Almagro G., Montero M., Etxeberria E., Hidalgo M., Sesma M.T., Pozueta-Romero J.
    Proc. Natl. Acad. Sci. U.S.A. 109:321-326(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiSUS3_ARATH
AccessioniPrimary (citable) accession number: Q9M111
Secondary accession number(s): Q9SBD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.