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Q9M111

- SUS3_ARATH

UniProt

Q9M111 - SUS3_ARATH

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Protein
Sucrose synthase 3
Gene
SUS3, At4g02280, T2H3.8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Modulates metabolic homeostasis and direct carbon towards starch synthesis in developing seeds.1 Publication

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.

Kineticsi

  1. KM=41.96 mM for D-fructose (synthetic reaction) at pH 9.4 (1 Publication)2 Publications
  2. KM=0.2 mM for UDP-glucose (synthetic reaction) at pH 9.4 (1 Publication)
  3. KM=108.2 mM for sucrose (degradative reaction) at pH 6 (1 Publication)
  4. KM=48 mM for sucrose (degradative reaction) at pH 7 (1 Publication)
  5. KM=0.09 mM for UDP (degradative reaction) at pH 6 (1 Publication)
  6. KM=0.25 mM for UDP (degradative reaction) at pH 7 (1 Publication)
  7. KM=0.15 mM for ADP (degradative reaction) at pH 7 (1 Publication)

Vmax=7.32 µmol/min/mg enzyme for synthetic reaction at pH 9.4 (1 Publication

)

Vmax=3.03 µmol/min/mg enzyme for degradative reaction at pH 6 (1 Publication

)

Vmax=950 µmol/min/mg enzyme for degradative reaction at pH 7 (1 Publication

)

pH dependencei

Optimum pH is 6.0-7.0 for degradative reaction and 7.0 (1 Publication) or 9.0-9.5 (1 Publication) for synthetic reaction.

GO - Molecular functioni

  1. sucrose synthase activity Source: UniProtKB

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. response to mannitol Source: UniProtKB
  3. response to water deprivation Source: UniProtKB
  4. seed maturation Source: TAIR
  5. starch metabolic process Source: TAIR
  6. sucrose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciMetaCyc:AT4G02280-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose synthase 3 (EC:2.4.1.13)
Short name:
AtSUS3
Alternative name(s):
Sucrose-UDP glucosyltransferase 3
Gene namesi
Name:SUS3
Ordered Locus Names:At4g02280
ORF Names:T2H3.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G02280.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Diminution of the starch content of developing seeds and increased lipid accumulation early during seed development.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Sucrose synthase 3
PRO_0000418802Add
BLAST

Proteomic databases

PRIDEiQ9M111.

Expressioni

Tissue specificityi

Detected in the whole plant with highest expression in developing siliques, vasculature of cotyledons and stomatal guard cells. Also detected throughout the mature parts of the root but not in the expanding zone.3 Publications

Developmental stagei

Highly expressed in late-maturing seeds and in geminating seeds (at the protein level).2 Publications

Inductioni

By drought stress. By mannitol, an osmotic agent. Positively regulated by LEC2.2 Publications

Gene expression databases

ArrayExpressiQ9M111.
GenevestigatoriQ9M111.

Structurei

3D structure databases

ProteinModelPortaliQ9M111.
SMRiQ9M111. Positions 25-808.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 755479GT-B glycosyltransferase By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000240125.
InParanoidiQ9M111.
KOiK00695.
OMAiFERCKED.
PhylomeDBiQ9M111.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PANTHERiPTHR12526:SF27. PTHR12526:SF27. 1 hit.
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M111-1 [UniParc]FASTAAdd to Basket

« Hide

MANPKLTRVL STRDRVQDTL SAHRNELVAL LSRYVDQGKG ILQPHNLIDE    50
LESVIGDDET KKSLSDGPFG EILKSAMEAI VVPPFVALAV RPRPGVWEYV 100
RVNVFELSVE QLTVSEYLRF KEELVDGPNS DPFCLELDFE PFNANVPRPS 150
RSSSIGNGVQ FLNRHLSSVM FRNKDCLEPL LDFLRVHKYK GHPLMLNDRI 200
QSISRLQIQL SKAEDHISKL SQETPFSEFE YALQGMGFEK GWGDTAGRVL 250
EMMHLLSDIL QAPDPSSLEK FLGMVPMVFN VVILSPHGYF GQANVLGLPD 300
TGGQVVYILD QVRALETEML LRIKRQGLDI SPSILIVTRL IPDAKGTTCN 350
QRLERVSGTE HTHILRVPFR SEKGILRKWI SRFDVWPYLE NYAQDAASEI 400
VGELQGVPDF IIGNYSDGNL VASLMAHRMG VTQCTIAHAL EKTKYPDSDI 450
YWKDFDNKYH FSCQFTADLI AMNNADFIIT STYQEIAGTK NTVGQYESHG 500
AFTLPGLYRV VHGIDVFDPK FNIVSPGADM TIYFPYSEET RRLTALHGSI 550
EEMLYSPDQT DEHVGTLSDR SKPILFSMAR LDKVKNISGL VEMYSKNTKL 600
RELVNLVVIA GNIDVNKSKD REEIVEIEKM HNLMKNYKLD GQFRWITAQT 650
NRARNGELYR YIADTRGAFA QPAFYEAFGL TVVEAMTCGL PTFATCHGGP 700
AEIIEHGLSG FHIDPYHPEQ AGNIMADFFE RCKEDPNHWK KVSDAGLQRI 750
YERYTWKIYS ERLMTLAGVY GFWKYVSKLE RRETRRYLEM FYILKFRDLV 800
KTVPSTADD 809
Length:809
Mass (Da):92,002
Last modified:October 1, 2000 - v1
Checksum:iBC5151F2FBF265D5
GO

Sequence cautioni

The sequence AAC28175.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF075597 Genomic DNA. Translation: AAC28175.1. Sequence problems.
AL161494 Genomic DNA. Translation: CAB80721.1.
CP002687 Genomic DNA. Translation: AEE82150.1.
AY051001 mRNA. Translation: AAK93678.1.
AY056784 mRNA. Translation: AAL09730.1.
AY142511 mRNA. Translation: AAN13112.1.
PIRiB85029.
T01420.
RefSeqiNP_192137.1. NM_116461.3.
UniGeneiAt.3877.

Genome annotation databases

EnsemblPlantsiAT4G02280.1; AT4G02280.1; AT4G02280.
GeneIDi828081.
KEGGiath:AT4G02280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF075597 Genomic DNA. Translation: AAC28175.1 . Sequence problems.
AL161494 Genomic DNA. Translation: CAB80721.1 .
CP002687 Genomic DNA. Translation: AEE82150.1 .
AY051001 mRNA. Translation: AAK93678.1 .
AY056784 mRNA. Translation: AAL09730.1 .
AY142511 mRNA. Translation: AAN13112.1 .
PIRi B85029.
T01420.
RefSeqi NP_192137.1. NM_116461.3.
UniGenei At.3877.

3D structure databases

ProteinModelPortali Q9M111.
SMRi Q9M111. Positions 25-808.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEi Q9M111.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G02280.1 ; AT4G02280.1 ; AT4G02280 .
GeneIDi 828081.
KEGGi ath:AT4G02280.

Organism-specific databases

TAIRi AT4G02280.

Phylogenomic databases

HOGENOMi HOG000240125.
InParanoidi Q9M111.
KOi K00695.
OMAi FERCKED.
PhylomeDBi Q9M111.

Enzyme and pathway databases

BioCyci MetaCyc:AT4G02280-MONOMER.

Gene expression databases

ArrayExpressi Q9M111.
Genevestigatori Q9M111.

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view ]
PANTHERi PTHR12526:SF27. PTHR12526:SF27. 1 hit.
Pfami PF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02470. sucr_synth. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Structure and expression profile of the sucrose synthase multigene family in Arabidopsis."
    Baud S., Vaultier M.N., Rochat C.
    J. Exp. Bot. 55:397-409(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  5. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. "Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development."
    Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.
    J. Exp. Bot. 59:3283-3295(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis and direct carbon towards starch synthesis in developing seeds."
    Angeles-Nunez J.G., Tiessen A.
    Planta 232:701-718(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Regulation of AtSUS2 and AtSUS3 by glucose and the transcription factor LEC2 in different tissues and at different stages of Arabidopsis seed development."
    Angeles-Nunez J.G., Tiessen A.
    Plant Mol. Biol. 78:377-392(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LEC2.
  9. "Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production."
    Baroja-Fernandez E., Munoz F.J., Li J., Bahaji A., Almagro G., Montero M., Etxeberria E., Hidalgo M., Sesma M.T., Pozueta-Romero J.
    Proc. Natl. Acad. Sci. U.S.A. 109:321-326(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiSUS3_ARATH
AccessioniPrimary (citable) accession number: Q9M111
Secondary accession number(s): Q9SBD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi