SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9M0Y8

- NSF_ARATH

UniProt

Q9M0Y8 - NSF_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Vesicle-fusing ATPase
Gene
NSF, At4g04910, T1J1.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in vesicle-mediated transport. The ATPase activity of NSF serves to disassemble the SNARE complex, freeing the components for subsequent pairing and fusion events.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi544 – 5441Magnesium

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi539 – 5468ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleoside-triphosphatase activity Source: InterPro
  4. protein binding Source: TAIR

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G04910-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:NSF
Ordered Locus Names:At4g04910
ORF Names:T1J1.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G04910.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. plasma membrane Source: TAIR
  3. plasmodesma Source: TAIR
  4. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 742742Vesicle-fusing ATPase
PRO_0000084568Add
BLAST

Proteomic databases

PaxDbiQ9M0Y8.
PRIDEiQ9M0Y8.

Expressioni

Gene expression databases

GenevestigatoriQ9M0Y8.

Interactioni

Subunit structurei

Hexamer. Binds to SNARE-SNAP complexes to form 20S particles By similarity.

Protein-protein interaction databases

BioGridi11141. 2 interactions.
IntActiQ9M0Y8. 4 interactions.
MINTiMINT-8061068.
STRINGi3702.AT4G04910.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M0Y8.
SMRiQ9M0Y8. Positions 1-708.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000198544.
InParanoidiQ9M0Y8.
KOiK06027.
OMAiMTIELEF.
PhylomeDBiQ9M0Y8.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M0Y8-1 [UniParc]FASTAAdd to Basket

« Hide

MAGRYGSQVM TMTVTNTPSA DLAFTNLAYC SSSDLRQFSV PGSDLFLANV    50
ADSFILSLCG HGSIRDGNIA LNAIQRRHAR VSTGDMVSVS RFVPPENFDL 100
AMLTLELEFV KKGTKSEQVD AALLSTQLKR KYTNQVLTVG QKATFEYHGT 150
NYILTVNRAD VEGQDHTNGI ERGLLSKDTY IVFEASNASG IKIVNQREAA 200
SSNIFKHKEF NLESLGIGGL GAEFADIFRR AFASRVFPPH VTSRLGIKHV 250
KGMLLFGPPG TGKTLMARQI GKMLNGKDPK IVNGPEVLSK FVGETEKNVR 300
DLFADAEQDQ RTLGDASELH VIIFDEIDAI CKSRGSTRDG TGVHDSIVNQ 350
LLTKIDGVEA LNNVLLIGMT NRKDLLDEAL LRPGRLEVQV EISLPDEAGR 400
LQILQIHTNK MKENSFLGTD INLQELAART KNYSGAELEG VVKSATSYAL 450
NRQLSMDDLT KPVEEENIKI TMEDFLHAIY EVQPAFGAST DDLERCRLNG 500
MVDCGHRHNH IYKRAMLLVE QVKVSTRSPL VTCLLEGPSG SGKTALAATI 550
GIDSDFPYVK IVSAETMIGL SESTKCAHIV KVFEDAYKSP MSIIILDDIE 600
RLLEFIAIGP RFSNIISQTL MVLLKRLPPK GKKLLVFGTT SEVTFLESVG 650
ISDCFSVTHS VPTLQKEDAK KVLNQLNLFS EDDVDSAAEA LNDMPIKKIY 700
MLIEMAAQGE NGGSAEAIYA GREKININHF YDCLGDFIRF TG 742
Length:742
Mass (Da):81,487
Last modified:February 1, 2003 - v2
Checksum:i39611058DEAC4A32
GO

Sequence cautioni

The sequence AAD17345.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB81033.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF128393 Genomic DNA. Translation: AAD17345.1. Sequence problems.
AL161502 Genomic DNA. Translation: CAB81033.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82440.1.
AY102111 mRNA. Translation: AAM26681.1.
PIRiG85061.
RefSeqiNP_192400.2. NM_116729.3.
UniGeneiAt.33942.
At.67369.

Genome annotation databases

EnsemblPlantsiAT4G04910.1; AT4G04910.1; AT4G04910.
GeneIDi825830.
KEGGiath:AT4G04910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF128393 Genomic DNA. Translation: AAD17345.1 . Sequence problems.
AL161502 Genomic DNA. Translation: CAB81033.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE82440.1 .
AY102111 mRNA. Translation: AAM26681.1 .
PIRi G85061.
RefSeqi NP_192400.2. NM_116729.3.
UniGenei At.33942.
At.67369.

3D structure databases

ProteinModelPortali Q9M0Y8.
SMRi Q9M0Y8. Positions 1-708.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 11141. 2 interactions.
IntActi Q9M0Y8. 4 interactions.
MINTi MINT-8061068.
STRINGi 3702.AT4G04910.1-P.

Proteomic databases

PaxDbi Q9M0Y8.
PRIDEi Q9M0Y8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G04910.1 ; AT4G04910.1 ; AT4G04910 .
GeneIDi 825830.
KEGGi ath:AT4G04910.

Organism-specific databases

GeneFarmi 2444. 154.
TAIRi AT4G04910.

Phylogenomic databases

eggNOGi COG0464.
HOGENOMi HOG000198544.
InParanoidi Q9M0Y8.
KOi K06027.
OMAi MTIELEF.
PhylomeDBi Q9M0Y8.

Enzyme and pathway databases

BioCyci ARA:AT4G04910-MONOMER.

Miscellaneous databases

PROi Q9M0Y8.

Gene expression databases

Genevestigatori Q9M0Y8.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiNSF_ARATH
AccessioniPrimary (citable) accession number: Q9M0Y8
Secondary accession number(s): Q9ZPH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi