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Protein

Vesicle-fusing ATPase

Gene

NSF

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in vesicle-mediated transport. The ATPase activity of NSF serves to disassemble the SNARE complex, freeing the components for subsequent pairing and fusion events.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi544MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi499 – 504ATPBy similarity6
Nucleotide bindingi539 – 546ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processER-Golgi transport, Protein transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G04910-MONOMER
ReactomeiR-ATH-204005 COPII-mediated vesicle transport
R-ATH-6807878 COPI-mediated anterograde transport
R-ATH-6811438 Intra-Golgi traffic
R-ATH-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:NSF
Ordered Locus Names:At4g04910
ORF Names:T1J1.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G04910
TAIRilocus:2135378 AT4G04910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845681 – 742Vesicle-fusing ATPaseAdd BLAST742

Proteomic databases

PaxDbiQ9M0Y8
PRIDEiQ9M0Y8

PTM databases

iPTMnetiQ9M0Y8

Expressioni

Gene expression databases

ExpressionAtlasiQ9M0Y8 baseline and differential
GenevisibleiQ9M0Y8 AT

Interactioni

Subunit structurei

Hexamer. Binds to SNARE-SNAP complexes to form 20S particles (By similarity).By similarity

Protein-protein interaction databases

BioGridi11141, 8 interactors
IntActiQ9M0Y8, 4 interactors
STRINGi3702.AT4G04910.1

Structurei

3D structure databases

ProteinModelPortaliQ9M0Y8
SMRiQ9M0Y8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741 Eukaryota
COG0464 LUCA
HOGENOMiHOG000198544
InParanoidiQ9M0Y8
KOiK06027
OMAiIDWVPIG
OrthoDBiEOG093604B5
PhylomeDBiQ9M0Y8

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRYGSQVM TMTVTNTPSA DLAFTNLAYC SSSDLRQFSV PGSDLFLANV
60 70 80 90 100
ADSFILSLCG HGSIRDGNIA LNAIQRRHAR VSTGDMVSVS RFVPPENFDL
110 120 130 140 150
AMLTLELEFV KKGTKSEQVD AALLSTQLKR KYTNQVLTVG QKATFEYHGT
160 170 180 190 200
NYILTVNRAD VEGQDHTNGI ERGLLSKDTY IVFEASNASG IKIVNQREAA
210 220 230 240 250
SSNIFKHKEF NLESLGIGGL GAEFADIFRR AFASRVFPPH VTSRLGIKHV
260 270 280 290 300
KGMLLFGPPG TGKTLMARQI GKMLNGKDPK IVNGPEVLSK FVGETEKNVR
310 320 330 340 350
DLFADAEQDQ RTLGDASELH VIIFDEIDAI CKSRGSTRDG TGVHDSIVNQ
360 370 380 390 400
LLTKIDGVEA LNNVLLIGMT NRKDLLDEAL LRPGRLEVQV EISLPDEAGR
410 420 430 440 450
LQILQIHTNK MKENSFLGTD INLQELAART KNYSGAELEG VVKSATSYAL
460 470 480 490 500
NRQLSMDDLT KPVEEENIKI TMEDFLHAIY EVQPAFGAST DDLERCRLNG
510 520 530 540 550
MVDCGHRHNH IYKRAMLLVE QVKVSTRSPL VTCLLEGPSG SGKTALAATI
560 570 580 590 600
GIDSDFPYVK IVSAETMIGL SESTKCAHIV KVFEDAYKSP MSIIILDDIE
610 620 630 640 650
RLLEFIAIGP RFSNIISQTL MVLLKRLPPK GKKLLVFGTT SEVTFLESVG
660 670 680 690 700
ISDCFSVTHS VPTLQKEDAK KVLNQLNLFS EDDVDSAAEA LNDMPIKKIY
710 720 730 740
MLIEMAAQGE NGGSAEAIYA GREKININHF YDCLGDFIRF TG
Length:742
Mass (Da):81,487
Last modified:February 1, 2003 - v2
Checksum:i39611058DEAC4A32
GO

Sequence cautioni

The sequence AAD17345 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81033 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128393 Genomic DNA Translation: AAD17345.1 Sequence problems.
AL161502 Genomic DNA Translation: CAB81033.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82440.1
AY102111 mRNA Translation: AAM26681.1
PIRiG85061
RefSeqiNP_192400.2, NM_116729.4
UniGeneiAt.33942
At.67369

Genome annotation databases

EnsemblPlantsiAT4G04910.1; AT4G04910.1; AT4G04910
GeneIDi825830
GrameneiAT4G04910.1; AT4G04910.1; AT4G04910
KEGGiath:AT4G04910

Similar proteinsi

Entry informationi

Entry nameiNSF_ARATH
AccessioniPrimary (citable) accession number: Q9M0Y8
Secondary accession number(s): Q9ZPH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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