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Q9M0Y8

- NSF_ARATH

UniProt

Q9M0Y8 - NSF_ARATH

Protein

Vesicle-fusing ATPase

Gene

NSF

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Feb 2003)
      Previous versions | rss
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    Functioni

    Involved in vesicle-mediated transport. The ATPase activity of NSF serves to disassemble the SNARE complex, freeing the components for subsequent pairing and fusion events.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi544 – 5441Magnesium

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi539 – 5468ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleoside-triphosphatase activity Source: InterPro
    4. protein binding Source: TAIR

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G04910-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
    Vesicular-fusion protein NSF
    Gene namesi
    Name:NSF
    Ordered Locus Names:At4g04910
    ORF Names:T1J1.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G04910.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: TAIR
    2. plasma membrane Source: TAIR
    3. plasmodesma Source: TAIR
    4. vacuole Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 742742Vesicle-fusing ATPasePRO_0000084568Add
    BLAST

    Proteomic databases

    PaxDbiQ9M0Y8.
    PRIDEiQ9M0Y8.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9M0Y8.

    Interactioni

    Subunit structurei

    Hexamer. Binds to SNARE-SNAP complexes to form 20S particles By similarity.By similarity

    Protein-protein interaction databases

    BioGridi11141. 2 interactions.
    IntActiQ9M0Y8. 4 interactions.
    MINTiMINT-8061068.
    STRINGi3702.AT4G04910.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M0Y8.
    SMRiQ9M0Y8. Positions 1-708.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    HOGENOMiHOG000198544.
    InParanoidiQ9M0Y8.
    KOiK06027.
    OMAiMTIELEF.
    PhylomeDBiQ9M0Y8.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9M0Y8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRYGSQVM TMTVTNTPSA DLAFTNLAYC SSSDLRQFSV PGSDLFLANV    50
    ADSFILSLCG HGSIRDGNIA LNAIQRRHAR VSTGDMVSVS RFVPPENFDL 100
    AMLTLELEFV KKGTKSEQVD AALLSTQLKR KYTNQVLTVG QKATFEYHGT 150
    NYILTVNRAD VEGQDHTNGI ERGLLSKDTY IVFEASNASG IKIVNQREAA 200
    SSNIFKHKEF NLESLGIGGL GAEFADIFRR AFASRVFPPH VTSRLGIKHV 250
    KGMLLFGPPG TGKTLMARQI GKMLNGKDPK IVNGPEVLSK FVGETEKNVR 300
    DLFADAEQDQ RTLGDASELH VIIFDEIDAI CKSRGSTRDG TGVHDSIVNQ 350
    LLTKIDGVEA LNNVLLIGMT NRKDLLDEAL LRPGRLEVQV EISLPDEAGR 400
    LQILQIHTNK MKENSFLGTD INLQELAART KNYSGAELEG VVKSATSYAL 450
    NRQLSMDDLT KPVEEENIKI TMEDFLHAIY EVQPAFGAST DDLERCRLNG 500
    MVDCGHRHNH IYKRAMLLVE QVKVSTRSPL VTCLLEGPSG SGKTALAATI 550
    GIDSDFPYVK IVSAETMIGL SESTKCAHIV KVFEDAYKSP MSIIILDDIE 600
    RLLEFIAIGP RFSNIISQTL MVLLKRLPPK GKKLLVFGTT SEVTFLESVG 650
    ISDCFSVTHS VPTLQKEDAK KVLNQLNLFS EDDVDSAAEA LNDMPIKKIY 700
    MLIEMAAQGE NGGSAEAIYA GREKININHF YDCLGDFIRF TG 742
    Length:742
    Mass (Da):81,487
    Last modified:February 1, 2003 - v2
    Checksum:i39611058DEAC4A32
    GO

    Sequence cautioni

    The sequence AAD17345.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB81033.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF128393 Genomic DNA. Translation: AAD17345.1. Sequence problems.
    AL161502 Genomic DNA. Translation: CAB81033.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82440.1.
    AY102111 mRNA. Translation: AAM26681.1.
    PIRiG85061.
    RefSeqiNP_192400.2. NM_116729.3.
    UniGeneiAt.33942.
    At.67369.

    Genome annotation databases

    EnsemblPlantsiAT4G04910.1; AT4G04910.1; AT4G04910.
    GeneIDi825830.
    KEGGiath:AT4G04910.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF128393 Genomic DNA. Translation: AAD17345.1 . Sequence problems.
    AL161502 Genomic DNA. Translation: CAB81033.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82440.1 .
    AY102111 mRNA. Translation: AAM26681.1 .
    PIRi G85061.
    RefSeqi NP_192400.2. NM_116729.3.
    UniGenei At.33942.
    At.67369.

    3D structure databases

    ProteinModelPortali Q9M0Y8.
    SMRi Q9M0Y8. Positions 1-708.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 11141. 2 interactions.
    IntActi Q9M0Y8. 4 interactions.
    MINTi MINT-8061068.
    STRINGi 3702.AT4G04910.1-P.

    Proteomic databases

    PaxDbi Q9M0Y8.
    PRIDEi Q9M0Y8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G04910.1 ; AT4G04910.1 ; AT4G04910 .
    GeneIDi 825830.
    KEGGi ath:AT4G04910.

    Organism-specific databases

    GeneFarmi 2444. 154.
    TAIRi AT4G04910.

    Phylogenomic databases

    eggNOGi COG0464.
    HOGENOMi HOG000198544.
    InParanoidi Q9M0Y8.
    KOi K06027.
    OMAi MTIELEF.
    PhylomeDBi Q9M0Y8.

    Enzyme and pathway databases

    BioCyci ARA:AT4G04910-MONOMER.

    Miscellaneous databases

    PROi Q9M0Y8.

    Gene expression databases

    Genevestigatori Q9M0Y8.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiNSF_ARATH
    AccessioniPrimary (citable) accession number: Q9M0Y8
    Secondary accession number(s): Q9ZPH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: February 1, 2003
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3