Q9M0X9 (4CLL7_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 64.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 4-coumarate--CoA ligase-like 7 EC=6.2.1.- Alternative name(s): 4-coumarate--CoA ligase isoform 6 Short name=At4CL6 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 544 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Contributes to jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of its precursors. Ref.6 |
| Subcellular location | |
| Induction | By methyl jasmonate. Ref.6 |
| Domain | Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity By similarity. |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. |
| Biophysicochemical properties | Kinetic parameters: KM=9 µM for nonanoic acid |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis |
| Cellular component | Peroxisome |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | jasmonic acid biosynthetic process Inferred from direct assay Ref.6. Source: TAIR |
| Cellular component | peroxisome Inferred from direct assay Ref.6. Source: TAIR |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fatty-acyl-CoA synthase activityInferred from direct assay Ref.6. Source: TAIR ligase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 544 | 544 | 4-coumarate--CoA ligase-like 7 | PRO_0000299180 | |||||
Regions | |||||||||
| Nucleotide binding | 198 – 206 | 9 | ATP By similarity | ||||||
| Nucleotide binding | 339 – 344 | 6 | ATP By similarity | ||||||
| Region | 269 – 338 | 70 | SBD1 By similarity | ||||||
| Region | 339 – 403 | 65 | SBD2 By similarity | ||||||
| Motif | 542 – 544 | 3 | Microbody targeting signal Potential | ||||||
Sites | |||||||||
| Binding site | 424 | 1 | ATP By similarity | ||||||
| Binding site | 439 | 1 | ATP By similarity | ||||||
| Binding site | 530 | 1 | ATP By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases." Shockey J.M., Fulda M.S., Browse J. Plant Physiol. 132:1065-1076(2003) [PubMed: 12805634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION. Strain: cv. Wassilewskija. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase." Schneider K., Hoevel K., Witzel K., Hamberger B., Schomburg D., Kombrink E., Stuible H.-P. Proc. Natl. Acad. Sci. U.S.A. 100:8601-8606(2003) [PubMed: 12819348] [Abstract] Cited for: GENE FAMILY ORGANIZATION. |
| [6] | "A new type of peroxisomal acyl-coenzyme A synthetase from Arabidopsis thaliana has the catalytic capacity to activate biosynthetic precursors of jasmonic acid." Schneider K., Kienow L., Schmelzer E., Colby T., Bartsch M., Miersch O., Wasternack C., Kombrink E., Stuible H.-P. J. Biol. Chem. 280:13962-13972(2005) [PubMed: 15677481] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY250839 mRNA. Translation: AAP03022.1. AL161502 Genomic DNA. Translation: CAB81058.1. CP002687 Genomic DNA. Translation: AEE82486.1. AY091079 mRNA. Translation: AAM13899.1. AY122950 mRNA. Translation: AAM67483.1. |
| IPI | IPI00536809. |
| PIR | H85064. |
| RefSeq | NP_192425.1. NM_116755.4. |
| UniGene | At.33913. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LCI based on UniProtKB P08659. |
| ProteinModelPortal | Q9M0X9. |
| SMR | Q9M0X9. Positions 10-539. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9M0X9. |
Proteomic databases | |
| PRIDE | Q9M0X9. |
| ProMEX | Q9M0X9. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G05160.1; AT4G05160.1; AT4G05160. |
| GeneID | 825864. |
| GenomeReviews | Gene locus AT4G05160 in contig CT486007_GR. |
| KEGG | ath:AT4G05160. |
| NMPDR | fig|3702.1.peg.18335. |
Organism-specific databases | |
| TAIR | At4g05160. |
Phylogenomic databases | |
| eggNOG | KOG1176. |
| GeneTree | EPGT00070000028073. |
| HOGENOM | HBG547964. |
| InParanoid | Q9M0X9. |
| OMA | YRSLRPT. |
| PhylomeDB | Q9M0X9. |
| ProtClustDB | CLSN2685605. |
Gene expression databases | |
| Genevestigator | Q9M0X9. |
Family and domain databases | |
| InterPro | IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view] |
| KO | K01904. |
| Pfam | PF00501. AMP-binding. 1 hit. [Graphical view] |
| PROSITE | PS00455. AMP_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | 4CLL7_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9M0X9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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