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Protein

Isoamylase 3, chloroplastic

Gene

ISA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in starch catabolism. ISA3 removes different branches than ISA1-ISA2, namely short chains that prevent amylopectin crystallization. May be the debranching enzyme required to assist beta-amylases for starch degradation in leaves at night.3 Publications

Miscellaneous

Double mutant shows that ISA3 and PU1 have redundant function for starch degradation.

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.

Pathwayi: starch degradation

This protein is involved in the pathway starch degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway starch degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei428NucleophileBy similarity1
Active sitei465Proton donorBy similarity1
Sitei538Transition state stabilizerBy similarity1

GO - Molecular functioni

  • isoamylase activity Source: TAIR

GO - Biological processi

  • starch catabolic process Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT4G09020-MONOMER
MetaCyc:AT4G09020-MONOMER
BRENDAi3.2.1.68 399
UniPathwayiUPA00153

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 3, chloroplastic (EC:3.2.1.68)
Short name:
AtISA3
Gene namesi
Name:ISA3
Ordered Locus Names:At4g09020
ORF Names:F23J3.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G09020
TAIRilocus:2122343 AT4G09020

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Strong increase of the starch level in leaves at the end of both day and night periods, but no modification in water-soluble polysaccharides content. No alteration of the amylase-to-amylopectin ratio.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastSequence analysisAdd BLAST70
ChainiPRO_000037952971 – 764Isoamylase 3, chloroplasticAdd BLAST694

Proteomic databases

PaxDbiQ9M0S5
PRIDEiQ9M0S5

PTM databases

iPTMnetiQ9M0S5

Expressioni

Gene expression databases

ExpressionAtlasiQ9M0S5 baseline and differential
GenevisibleiQ9M0S5 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G09020.1

Structurei

3D structure databases

ProteinModelPortaliQ9M0S5
SMRiQ9M0S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
HOGENOMiHOG000239197
InParanoidiQ9M0S5
KOiK01214
OMAiSEPWDCG
OrthoDBiEOG093602GP
PhylomeDBiQ9M0S5

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M0S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR
60 70 80 90 100
KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
110 120 130 140 150
GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH
160 170 180 190 200
ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
210 220 230 240 250
DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE
260 270 280 290 300
SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
310 320 330 340 350
DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI
360 370 380 390 400
LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
410 420 430 440 450
NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA
460 470 480 490 500
IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
510 520 530 540 550
GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF
560 570 580 590 600
KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
610 620 630 640 650
SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS
660 670 680 690 700
EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
710 720 730 740 750
IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT
760
YNVAPFSSIL LQSK
Length:764
Mass (Da):86,322
Last modified:July 7, 2009 - v2
Checksum:iEE9F163AE7F5E052
GO

Sequence cautioni

The sequence CAB78026 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161513 Genomic DNA Translation: CAB78026.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82713.1
AY091058 mRNA Translation: AAM13879.1
AY133739 mRNA Translation: AAM91673.1
AK227049 mRNA Translation: BAE99109.1
PIRiB85091
RefSeqiNP_192641.2, NM_116971.6
UniGeneiAt.33717

Genome annotation databases

EnsemblPlantsiAT4G09020.1; AT4G09020.1; AT4G09020
GeneIDi826481
GrameneiAT4G09020.1; AT4G09020.1; AT4G09020
KEGGiath:AT4G09020

Similar proteinsi

Entry informationi

Entry nameiISOA3_ARATH
AccessioniPrimary (citable) accession number: Q9M0S5
Secondary accession number(s): Q8RWW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 7, 2009
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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