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Protein

Dehydration-responsive element-binding protein 1A

Gene

DREB1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi50 – 107AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cold acclimation Source: TAIR
  • response to cold Source: TAIR
  • response to water deprivation Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydration-responsive element-binding protein 1A1 Publication
Short name:
Protein DREB1A1 Publication
Alternative name(s):
C-repeat/dehydration-responsive element-binding factor 31 Publication
Short name:
C-repeat-binding factor 31 Publication
Short name:
CRT/DRE-binding factor 31 Publication
Cold resistance-related AP2 transcription factor
Gene namesi
Name:DREB1A1 Publication
Synonyms:CBF31 Publication, CRAP2, ERF0721 Publication
Ordered Locus Names:At4g25480Imported
ORF Names:M7J2.150Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G25480.
TAIRilocus:2131849. AT4G25480.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63V → A: Affects the binding to the CRT/DRE cis-element. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125281 – 216Dehydration-responsive element-binding protein 1AAdd BLAST216

Proteomic databases

PaxDbiQ9M0L0.

Expressioni

Inductioni

By cold stress. Positively regulated by the transcription factor ICE1. Subject to degradation by the 26S proteasome pathway in freezing conditions (PubMed:28344081).4 Publications

Gene expression databases

ExpressionAtlasiQ9M0L0. baseline and differential.
GenevisibleiQ9M0L0. AT.

Interactioni

Subunit structurei

Interacts with 14-3-3 proteins GRF1, GRF3, GRF5, GRF6, GRF7, GRF9 and GRF10 in the nucleus upon freezing.1 Publication

Protein-protein interaction databases

BioGridi13939. 3 interactors.
STRINGi3702.AT4G25480.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M0L0.
SMRiQ9M0L0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 47Nuclear localization signalSequence analysisAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi205 – 210Poly-Asp6

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IVY9. Eukaryota.
ENOG410YK37. LUCA.
InParanoidiQ9M0L0.
KOiK09286.
OMAiAFQDEMC.
OrthoDBiEOG09360N5C.
PhylomeDBiQ9M0L0.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiView protein in InterPro
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
PfamiView protein in Pfam
PF00847. AP2. 1 hit.
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiView protein in SMART
SM00380. AP2. 1 hit.
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiView protein in PROSITE
PS51032. AP2_ERF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M0L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSFSAFSEM FGSDYESSVS SGGDYIPTLA SSCPKKPAGR KKFRETRHPI
60 70 80 90 100
YRGVRRRNSG KWVCEVREPN KKTRIWLGTF QTAEMAARAH DVAALALRGR
110 120 130 140 150
SACLNFADSA WRLRIPESTC AKDIQKAAAE AALAFQDEMC DATTDHGFDM
160 170 180 190 200
EETLVEAIYT AEQSENAFYM HDEAMFEMPS LLANMAEGML LPLPSVQWNH
210
NHEVDGDDDD VSLWSY
Length:216
Mass (Da):24,236
Last modified:May 24, 2004 - v2
Checksum:iC625E2D0FAE0FFFB
GO

Sequence cautioni

The sequence ABD72616 differs from that shown. Reason: Erroneous termination at position 152. Translated as Glu.Curated
The sequence CAA18178 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81358 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146H → Y in BAA33434 (PubMed:9735350).Curated1
Sequence conflicti146H → Y in AAU93686 (Ref. 6) Curated1
Sequence conflicti146H → Y in ABD14412 (Ref. 7) Curated1
Sequence conflicti193L → P in ABD72616 (Ref. 8) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2N → S in strain: cv. Cvi-1. 1 Publication1
Natural varianti19V → I in strain: cv. Cvi-1. 1 Publication1
Natural varianti142A → V in strain: cv. Cvi-1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007787 mRNA. Translation: BAA33791.1.
AB013815 Genomic DNA. Translation: BAA33434.1.
AF074602 mRNA. Translation: AAD15977.1.
AF076155 Genomic DNA. Translation: AAC99370.1.
AF062924 Genomic DNA. Translation: AAC78646.1.
AY667247 Genomic DNA. Translation: AAV80414.1.
AY691904 Genomic DNA. Translation: AAU93686.1.
DQ372533 Genomic DNA. Translation: ABD14412.1.
DQ415923 Genomic DNA. Translation: ABD72616.1. Sequence problems.
AL022197 Genomic DNA. Translation: CAA18178.1. Sequence problems.
AL161563 Genomic DNA. Translation: CAB81358.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85065.1.
BT024594 mRNA. Translation: ABD42992.1.
PIRiD85294.
JE0297.
T05799.
T51830.
RefSeqiNP_567720.1. NM_118680.2.
UniGeneiAt.231.

Genome annotation databases

EnsemblPlantsiAT4G25480.1; AT4G25480.1; AT4G25480.
GeneIDi828652.
GrameneiAT4G25480.1; AT4G25480.1; AT4G25480.
KEGGiath:AT4G25480.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDRE1A_ARATH
AccessioniPrimary (citable) accession number: Q9M0L0
Secondary accession number(s): O65612
, O82131, Q1ZZS0, Q2HII7, Q5QE70, Q5Y4C4, Q9SAZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: June 7, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families