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Protein

Mannan endo-1,4-beta-mannosidase 5

Gene

MAN5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei214Proton donorBy similarity1
Active sitei334NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • mannan catabolic process Source: GO_Central
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT4G28320-MONOMER.
BRENDAi3.2.1.78. 399.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 5 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 5
Endo-beta-1,4-mannanase 5
Short name:
AtMAN5
Gene namesi
Name:MAN5
Ordered Locus Names:At4g28320
ORF Names:F26K10.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G28320.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000027747825 – 431Mannan endo-1,4-beta-mannosidase 5Add BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...)Sequence analysis1
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M0H6.
PRIDEiQ9M0H6.

PTM databases

iPTMnetiQ9M0H6.

Expressioni

Tissue specificityi

Expressed in stems.1 Publication

Gene expression databases

GenevisibleiQ9M0H6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G28320.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M0H6.
SMRiQ9M0H6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IKPI. Eukaryota.
COG3934. LUCA.
HOGENOMiHOG000169952.
InParanoidiQ9M0H6.
KOiK19355.
OMAiQDWIDEM.
OrthoDBiEOG09360BHK.
PhylomeDBiQ9M0H6.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M0H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPTRNRPML RILGFFICAA FIYLSFRDLW LNHKGKAKLG FVKRNGTQFV
60 70 80 90 100
VDDKPLYVNG WNSYWFMDHA VDEHSRNLVG EMLEAGAKMG LTVCRTWAFN
110 120 130 140 150
DGGYNALQIS PGRFDERVFQ ALDHVIAEAR KHDVRLLLSL VNNLQAYGGK
160 170 180 190 200
TQYVKWAWQE GVGLSSSNDS FFFDPSIRNY FKNYLKVLLT RKNSVTGIEY
210 220 230 240 250
RNDPTIFAWE LINEPRCTTD VSGKTLQDWI DEMTGFIKSI DDKHLLTVGL
260 270 280 290 300
EGFYGPNSPK GLTVNPEQWA SQLGTDFVQN SNSSNIDFAS VHIYPDHWFH
310 320 330 340 350
NQTFEEKLKF VVKWMQSHIE DGLKELKKPV LFTEFGLSNQ NKDYEPSQRD
360 370 380 390 400
KFYRIIFDVV YKSAKRKKSG AGTLVWQLFM EGMETFNDDF GIVPHEQDSI
410 420 430
YKLMIEQSCR LGKVTGRLKE QKLKELCSHR H
Length:431
Mass (Da):49,899
Last modified:October 1, 2000 - v1
Checksum:i3EEDCC0BE61EC90A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161572 Genomic DNA. Translation: CAB79634.1.
CP002687 Genomic DNA. Translation: AEE85469.1.
AK228732 mRNA. Translation: BAF00634.1.
BT026124 mRNA. Translation: ABG48480.1.
PIRiT09048.
RefSeqiNP_194561.1. NM_118972.5.
UniGeneiAt.48910.
At.71248.

Genome annotation databases

EnsemblPlantsiAT4G28320.1; AT4G28320.1; AT4G28320.
GeneIDi828947.
GrameneiAT4G28320.1; AT4G28320.1; AT4G28320.
KEGGiath:AT4G28320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161572 Genomic DNA. Translation: CAB79634.1.
CP002687 Genomic DNA. Translation: AEE85469.1.
AK228732 mRNA. Translation: BAF00634.1.
BT026124 mRNA. Translation: ABG48480.1.
PIRiT09048.
RefSeqiNP_194561.1. NM_118972.5.
UniGeneiAt.48910.
At.71248.

3D structure databases

ProteinModelPortaliQ9M0H6.
SMRiQ9M0H6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G28320.1.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

PTM databases

iPTMnetiQ9M0H6.

Proteomic databases

PaxDbiQ9M0H6.
PRIDEiQ9M0H6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G28320.1; AT4G28320.1; AT4G28320.
GeneIDi828947.
GrameneiAT4G28320.1; AT4G28320.1; AT4G28320.
KEGGiath:AT4G28320.

Organism-specific databases

TAIRiAT4G28320.

Phylogenomic databases

eggNOGiENOG410IKPI. Eukaryota.
COG3934. LUCA.
HOGENOMiHOG000169952.
InParanoidiQ9M0H6.
KOiK19355.
OMAiQDWIDEM.
OrthoDBiEOG09360BHK.
PhylomeDBiQ9M0H6.

Enzyme and pathway databases

BioCyciARA:AT4G28320-MONOMER.
BRENDAi3.2.1.78. 399.

Miscellaneous databases

PROiQ9M0H6.

Gene expression databases

GenevisibleiQ9M0H6. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAN5_ARATH
AccessioniPrimary (citable) accession number: Q9M0H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.