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Protein

Mannan endo-1,4-beta-mannosidase 5

Gene

MAN5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei214Proton donorBy similarity1
Active sitei334NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • mannan catabolic process Source: GO_Central
  • seed germination Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT4G28320-MONOMER.
BRENDAi3.2.1.78. 399.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 5 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 5
Endo-beta-1,4-mannanase 5
Short name:
AtMAN5
Gene namesi
Name:MAN5
Ordered Locus Names:At4g28320
ORF Names:F26K10.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G28320.
TAIRilocus:2123628. AT4G28320.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000027747825 – 431Mannan endo-1,4-beta-mannosidase 5Add BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M0H6.
PRIDEiQ9M0H6.

PTM databases

iPTMnetiQ9M0H6.

Expressioni

Tissue specificityi

Expressed in stems.1 Publication

Gene expression databases

ExpressionAtlasiQ9M0H6. baseline and differential.
GenevisibleiQ9M0H6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G28320.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M0H6.
SMRiQ9M0H6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IKPI. Eukaryota.
COG3934. LUCA.
HOGENOMiHOG000169952.
InParanoidiQ9M0H6.
KOiK19355.
OMAiEEYGWTD.
OrthoDBiEOG09360BHK.
PhylomeDBiQ9M0H6.

Family and domain databases

InterProiView protein in InterPro
IPR001547. Glyco_hydro_5.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00150. Cellulase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M0H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPTRNRPML RILGFFICAA FIYLSFRDLW LNHKGKAKLG FVKRNGTQFV
60 70 80 90 100
VDDKPLYVNG WNSYWFMDHA VDEHSRNLVG EMLEAGAKMG LTVCRTWAFN
110 120 130 140 150
DGGYNALQIS PGRFDERVFQ ALDHVIAEAR KHDVRLLLSL VNNLQAYGGK
160 170 180 190 200
TQYVKWAWQE GVGLSSSNDS FFFDPSIRNY FKNYLKVLLT RKNSVTGIEY
210 220 230 240 250
RNDPTIFAWE LINEPRCTTD VSGKTLQDWI DEMTGFIKSI DDKHLLTVGL
260 270 280 290 300
EGFYGPNSPK GLTVNPEQWA SQLGTDFVQN SNSSNIDFAS VHIYPDHWFH
310 320 330 340 350
NQTFEEKLKF VVKWMQSHIE DGLKELKKPV LFTEFGLSNQ NKDYEPSQRD
360 370 380 390 400
KFYRIIFDVV YKSAKRKKSG AGTLVWQLFM EGMETFNDDF GIVPHEQDSI
410 420 430
YKLMIEQSCR LGKVTGRLKE QKLKELCSHR H
Length:431
Mass (Da):49,899
Last modified:October 1, 2000 - v1
Checksum:i3EEDCC0BE61EC90A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161572 Genomic DNA. Translation: CAB79634.1.
CP002687 Genomic DNA. Translation: AEE85469.1.
AK228732 mRNA. Translation: BAF00634.1.
BT026124 mRNA. Translation: ABG48480.1.
PIRiT09048.
RefSeqiNP_194561.1. NM_118972.5.
UniGeneiAt.48910.
At.71248.

Genome annotation databases

EnsemblPlantsiAT4G28320.1; AT4G28320.1; AT4G28320.
GeneIDi828947.
GrameneiAT4G28320.1; AT4G28320.1; AT4G28320.
KEGGiath:AT4G28320.

Similar proteinsi

Entry informationi

Entry nameiMAN5_ARATH
AccessioniPrimary (citable) accession number: Q9M0H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome