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Protein

Serine/threonine-protein kinase VPS15

Gene

VPS15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I (By similarity). Required for pollen development and germination, probably via the modulation of phosphatidylinositol 3-phosphate (PI3P) formation and vacuolar organization (PubMed:21833541, PubMed:22361507).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 41ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • autophagy of peroxisome Source: GO_Central
  • late endosome to vacuole transport Source: GO_Central
  • macroautophagy Source: GO_Central
  • phosphatidylinositol-mediated signaling Source: UniProtKB
  • pollen development Source: TAIR
  • pollen germination Source: UniProtKB
  • protein phosphorylation Source: GO_Central
  • protein targeting to vacuole Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAutophagy, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-1632852. Macroautophagy.
R-ATH-1660514. Synthesis of PIPs at the Golgi membrane.
R-ATH-1660516. Synthesis of PIPs at the early endosome membrane.
R-ATH-1660517. Synthesis of PIPs at the late endosome membrane.
R-ATH-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase VPS151 Publication (EC:2.7.11.1PROSITE-ProRule annotation)
Alternative name(s):
Vacuolar protein sorting-associated protein 151 Publication
Short name:
AtVPS151 Publication
Gene namesi
Name:VPS151 Publication
Ordered Locus Names:At4g29380Imported
ORF Names:F17A13.200Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G29380.
TAIRilocus:2118234. AT4G29380.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced male gametophyte transmission. Abnormal pollen grains with unusual large vacuoles. Reduced pollen germination rescued by phosphatidylinositol 3-phosphate (PI3P) treatment.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00004419312 – 1494Serine/threonine-protein kinase VPS15Add BLAST1493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

PTM databases

iPTMnetiQ9M0E5.

Expressioni

Tissue specificityi

Mainly expressed in anthers, pollen grains and pollen tubes, and, to a lower extent, in other tissues and organs including seedlings, roots, stems, leaves, flowers, pitils and siliques.2 Publications

Developmental stagei

In anthers, barely detected in microsporocytes at stage 6, but accumulates strongly in tetrads at stage 7, microspores, mature pollen grains, and tapetum cells. Also detectable in the growing pollen tubes on the stigma.2 Publications

Gene expression databases

ExpressionAtlasiQ9M0E5. baseline and differential.

Interactioni

Subunit structurei

Interacts with VPS34.1 Publication

Protein-protein interaction databases

STRINGi3702.AT4G29380.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 307Protein kinasePROSITE-ProRule annotationAdd BLAST281
Repeati383 – 421HEAT 1Sequence analysisAdd BLAST39
Repeati480 – 517HEAT 2Sequence analysisAdd BLAST38
Repeati524 – 562HEAT 3Sequence analysisAdd BLAST39
Repeati610 – 646HEAT 4Sequence analysisAdd BLAST37
Repeati648 – 685HEAT 5Sequence analysisAdd BLAST38
Repeati687 – 724HEAT 6Sequence analysisAdd BLAST38
Repeati727 – 764HEAT 7Sequence analysisAdd BLAST38
Repeati1079 – 1118WD 1Sequence analysisAdd BLAST40
Repeati1127 – 1166WD 2Sequence analysisAdd BLAST40
Repeati1184 – 1226WD 3Sequence analysisAdd BLAST43
Repeati1231 – 1270WD 4Sequence analysisAdd BLAST40
Repeati1276 – 1323WD 5Sequence analysisAdd BLAST48
Repeati1371 – 1409WD 6Sequence analysisAdd BLAST39
Repeati1466 – 1494WD 7Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi953 – 1065Ser-richPROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1240. Eukaryota.
ENOG410XPDN. LUCA.
HOGENOMiHOG000030331.
OMAiWCILYPS.
OrthoDBiEOG09360091.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.130.10.10. 3 hits.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF02985. HEAT. 1 hit.
PF00069. Pkinase. 1 hit.
PF00400. WD40. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SM00320. WD40. 5 hits.
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF50978. SSF50978. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50077. HEAT_REPEAT. 2 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M0E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNKIARTTQ VSATEYYLHD LPSSYNLVLK EVLGRGRFLK SIQCKHDEGL
60 70 80 90 100
VVVKVYFKRG DSIDLREYER RLVKIKDVFL SLEHPHVWPF QFWQETDKAA
110 120 130 140 150
YLVRQYFYSN LHDRLSTRPF LSLVEKKWLA FQLLLAVKQC HEKDICHGDI
160 170 180 190 200
KCENVLLTSW NWLYLADFAS FKPTYIPYDD PSDFSFFFDT RGQRLCYLAP
210 220 230 240 250
ERFYEHGGET QVAQDAPLKP SMDIFAVGCV IAELFLEGQP LFELAQLLAY
260 270 280 290 300
RRGQHDPSQH LEKIPDPGIR KMILHMIQLE PEARLSAEDY LQNYVGVVFP
310 320 330 340 350
NYFSPFLHTL YCCWNPLPSD MRVATCQGIF QEILKKMMEN KSGDEIGVDS
360 370 380 390 400
PVTSNPMNAS TVQETFANHK LNSSKDLIRN TVNSKDEIFY SISDALKKNR
410 420 430 440 450
HPFLKKITMD DLGTLMSLYD SRSDTYGTPF LPVEGNMRCE GMVLIASMLC
460 470 480 490 500
SCIRNIKLPH LRREAILLLR SCSLYIDDDD RLQRVLPYVV ALLSDPTAIV
510 520 530 540 550
RCAAMETLCD ILPLVRDFPP SDAKIFPEYI FPMLSMLPED TEESVRICYA
560 570 580 590 600
SNIAKLALTA YGFLIHSFQL SDVGVLNELN SQQISTTPAS ETPSHLQKAN
610 620 630 640 650
GNAQLQQLRK TIAEVVQELV MGPKQTPNVR RALLQDIGEL CFFFGQRQSN
660 670 680 690 700
DFLLPILPAF LNDRDEQLRS VFFEKIVYVC FFVGQRSVEE YLLPYIDQAL
710 720 730 740 750
SDQTEAVIVN ALECLSTLCK SSFLRKRALL QMIECVYPLL CYPSQWVRRA
760 770 780 790 800
VVTFIAASSE CLGAVDSYAF IAPVIRSYLS RLPASIASEE GLLSCLKPPV
810 820 830 840 850
TREVVYRIFE KTRNPEFMAK QRKMWYSSSP QSKDWESVDL FDKDAGELNS
860 870 880 890 900
VECRAEQKQS VEGKKQIKSA SKQPEVQGKY AEKDAKLRIP RNPRPNASNT
910 920 930 940 950
VELRDPVYPE KLQFSGFMAP YVSGANSFIE PENIPLYSFS MDKRAATNPP
960 970 980 990 1000
VASESSLQMN SLGMGSLSVP WMDSMSKSFN LASSVPVPKL ISGSFHVGTN
1010 1020 1030 1040 1050
PKQFYRVVHE PESRENDQIS SAISKFQDLG VSSSSKSASV TSEDASSPAD
1060 1070 1080 1090 1100
LVGEPSLSRT SVPDSGWKPR GVLVAHLQEH RSAVNDIATS SDHSFFVSAS
1110 1120 1130 1140 1150
DDSTVKVWDS RKLEKDISFR SRLTYHLEGS RGMCTTMLRN STQVVVGASD
1160 1170 1180 1190 1200
GVIHMFSIDH ISRGLGNVVE KYSGIVDIKK KDVKEGALVS LLNYTADSLS
1210 1220 1230 1240 1250
GPMVMYSTQN CGIHLWDTRS DLDAWTLKAN PEEGYVSSLV TSPCGNWFVS
1260 1270 1280 1290 1300
GSSRGVLTLW DLRFRVPVNS WQYPIICPIE KMCLCFLPPS VSVSTTMKPL
1310 1320 1330 1340 1350
IYVAAGCNEV SLWNAEGGSC HQVLRVANYE NETDVSEFQW KLPSNKVNPK
1360 1370 1380 1390 1400
PNHRQNMSSK YRIEELNEPP PRLPGIRSLL PLPGGDLLTG GTDLKIRRWD
1410 1420 1430 1440 1450
YSSPERSYCI CGPSLKGVGN DDFYELKTNT GVQFVQETKR RPLATKLTAK
1460 1470 1480 1490
AVLAAAATDT AGCHRDSVQS LASVKLNQRL LISSSRDGAI KVWK
Length:1,494
Mass (Da):168,124
Last modified:October 1, 2000 - v1
Checksum:i78D71855073E8BFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161574 Genomic DNA. Translation: CAB79696.1.
CP002687 Genomic DNA. Translation: AEE85624.1.
RefSeqiNP_194667.1. NM_119083.3.
UniGeneiAt.27874.

Genome annotation databases

EnsemblPlantsiAT4G29380.1; AT4G29380.1; AT4G29380.
GeneIDi829059.
GrameneiAT4G29380.1; AT4G29380.1; AT4G29380.

Similar proteinsi

Entry informationi

Entry nameiVPS15_ARATH
AccessioniPrimary (citable) accession number: Q9M0E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2017
Last sequence update: October 1, 2000
Last modified: November 22, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families