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Protein

Xyloglucan endotransglucosylase/hydrolase protein 19

Gene

XTH19

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses xyloglucan endotransglucosylase (XET) activity in vitro. Does not possess xyloglucan endohydrolysis (XEH) activity (PubMed:20732879). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (PubMed:23585673). Involved in cell proliferation in the tissue reunion process of wounded inflorescence stems. Maybe a downstream target of NAC071 as a consequence of auxin action in wounded stems (PubMed:25182467).1 Publication2 Publications

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99NucleophilePROSITE-ProRule annotation1
Active sitei103Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: TAIR
  • cellular response to auxin stimulus Source: TAIR
  • cell wall biogenesis Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • xyloglucan metabolic process Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT4G30290-MONOMER
BRENDAi2.4.1.207 399

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 19Curated (EC:2.4.1.2071 Publication)
Short name:
At-XTH191 Publication
Short name:
XTH-191 Publication
Gene namesi
Name:XTH191 Publication
Ordered Locus Names:At4g30290
ORF Names:F17I23.370
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30290
TAIRilocus:2118751 AT4G30290

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001181922 – 277Xyloglucan endotransglucosylase/hydrolase protein 19Add BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9M0D1
PRIDEiQ9M0D1

Expressioni

Tissue specificityi

Root specific.1 Publication

Inductioni

By auxin.1 Publication

Gene expression databases

ExpressionAtlasiQ9M0D1 baseline and differential
GenevisibleiQ9M0D1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G30290.1

Structurei

3D structure databases

ProteinModelPortaliQ9M0D1
SMRiQ9M0D1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 213GH16PROSITE-ProRule annotationAdd BLAST192

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHDJ Eukaryota
COG2273 LUCA
HOGENOMiHOG000236368
InParanoidiQ9M0D1
KOiK08235
OMAiPANSQWF
OrthoDBiEOG093614W3
PhylomeDBiQ9M0D1

Family and domain databases

InterProiView protein in InterPro
IPR008264 Beta_glucanase
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
PRINTSiPR00737 GLHYDRLASE16
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M0D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFTFLILF LFAAQSISVY AGSFHKDVKI HWGDGRGKIH DNQGKLLSLS
60 70 80 90 100
LDKSSGSGFQ SNQEFLYGKA EVQMKLVPGN SAGTVTTFYL KSPGTTWDEI
110 120 130 140 150
DFEFLGNISG HPYTLHTNVY TKGSGDKEQQ FHLWFDPTAN FHTYCITWNP
160 170 180 190 200
QRIIFTVDGI PIREFMNAES RGVPFPTKQP MRLYASLWEA EHWATRGGLE
210 220 230 240 250
KTDWSKAPFT AYYRNYNVEG CVWVNGKSVC PANSQWFTQK LDSNGQTRMK
260 270
GVQSKYMVYN YCSDKKRFPR GVPPECS
Length:277
Mass (Da):31,563
Last modified:October 1, 2000 - v1
Checksum:iB801F67E0CD72E53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112P → T in AAK91391 (PubMed:14593172).Curated1
Sequence conflicti112P → T in AAN28826 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161576 Genomic DNA Translation: CAB81022.1
CP002687 Genomic DNA Translation: AEE85747.1
AY050373 mRNA Translation: AAK91391.1
AY143887 mRNA Translation: AAN28826.1
PIRiB85354
RefSeqiNP_194758.1, NM_119175.3
UniGeneiAt.23039

Genome annotation databases

EnsemblPlantsiAT4G30290.1; AT4G30290.1; AT4G30290
GeneIDi829152
GrameneiAT4G30290.1; AT4G30290.1; AT4G30290
KEGGiath:AT4G30290

Similar proteinsi

Entry informationi

Entry nameiXTH19_ARATH
AccessioniPrimary (citable) accession number: Q9M0D1
Secondary accession number(s): Q94A49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health