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Protein

UDP-glucuronate 4-epimerase 1

Gene

GAE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.1 Publication

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Enzyme regulationi

Inhibited by UDP-Xylose.

Kineticsi

Equilibrium between UDP-glucuronate and UDP-D-galacturonate established at 1:1.3. No activity with UDP-Galactose, UDP-Glucose or UDP-Xylose.
  1. KM=0.19 mM for UDP-glucuronate1 Publication

    pH dependencei

    Optimum pH is 7.6. Active from pH 6 to 8.9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei239Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi89 – 120NADBy similarityAdd BLAST32

    GO - Molecular functioni

    • UDP-glucuronate 4-epimerase activity Source: TAIR

    GO - Biological processi

    • defense response to fungus Source: TAIR
    • defense response to Gram-negative bacterium Source: TAIR
    • galacturonate biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionIsomerase
    Biological processCarbohydrate metabolism
    LigandNAD

    Enzyme and pathway databases

    BRENDAi5.1.3.6 399
    SABIO-RKiQ9M0B6

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronate 4-epimerase 1 (EC:5.1.3.6)
    Alternative name(s):
    UDP-glucuronic acid epimerase 1
    Short name:
    AtUGlcAE3
    Gene namesi
    Name:GAE1
    Synonyms:UGlcAE3
    Ordered Locus Names:At4g30440
    ORF Names:F17I23.220
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G30440
    TAIRilocus:2118711 AT4G30440

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
    Transmembranei87 – 107HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002925961 – 429UDP-glucuronate 4-epimerase 1Add BLAST429

    Proteomic databases

    PaxDbiQ9M0B6

    PTM databases

    iPTMnetiQ9M0B6
    SwissPalmiQ9M0B6

    Expressioni

    Tissue specificityi

    In root stele, leaves, siliques, flowers, pollen and stems.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9M0B6 baseline and differential
    GenevisibleiQ9M0B6 AT

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G30440.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M0B6
    SMRiQ9M0B6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi213 – 216Poly-Ser4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IU3F Eukaryota
    COG0451 LUCA
    HOGENOMiHOG000168000
    InParanoidiQ9M0B6
    KOiK08679
    OMAiWHTTAST
    OrthoDBiEOG09360AZR
    PhylomeDBiQ9M0B6

    Family and domain databases

    InterProiView protein in InterPro
    IPR016040 NAD(P)-bd_dom
    IPR036291 NAD(P)-bd_dom_sf
    IPR008089 Nuc_sugar_epim
    PfamiView protein in Pfam
    PF16363 GDP_Man_Dehyd, 1 hit
    PRINTSiPR01713 NUCEPIMERASE
    SUPFAMiSSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9M0B6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSIEDELFP STPGKFKIDR SNRQLHRCFA STSTMFLWAL FLIALTASYL
    60 70 80 90 100
    SFQSFVDSGS RYLTASWGGI QWEKQVRTSA QIHRSGGISV LVTGATGFVG
    110 120 130 140 150
    SHVSLALRKR GDGVVGLDNF NNYYDPSLKR ARRSLLSSRG IFVVEGDLND
    160 170 180 190 200
    AKLLAKLFDV VAFTHVMHLA AQAGVRYALE NPQSYVHSNI AGLVNLLEIC
    210 220 230 240 250
    KAANPQPAIV WASSSSVYGL NEKVPFSESD RTDQPASLYA ATKKAGEEIT
    260 270 280 290 300
    HTYNHIYGLA ITGLRFFTVY GPWGRPDMAY FSFTRNILQG KPITIYRGKN
    310 320 330 340 350
    RVDLARDFTY IDDIVKGCLG SLDSSGKSTG SGGKKRGAAP YRIFNLGNTS
    360 370 380 390 400
    PVTVPILVDI LEKHLKVKAK RNFVEMPGNG DVPFTHANIS SARNEFGYKP
    410 420
    TTDLETGLKK FVRWYLSYYG YNTKAKLVH
    Length:429
    Mass (Da):47,458
    Last modified:October 1, 2000 - v1
    Checksum:i1FDD14BC3FDABCD4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY661562 Genomic DNA Translation: AAT77233.1
    AL161577 Genomic DNA Translation: CAB79762.1
    CP002687 Genomic DNA Translation: AEE85765.1
    AY056303 mRNA Translation: AAL07152.1
    AY099855 mRNA Translation: AAM20706.1
    BT000308 mRNA Translation: AAN15627.1
    AY085505 mRNA Translation: AAM62729.1
    PIRiA85356
    RefSeqiNP_194773.1, NM_119190.4
    UniGeneiAt.20969
    At.67043

    Genome annotation databases

    EnsemblPlantsiAT4G30440.1; AT4G30440.1; AT4G30440
    GeneIDi829167
    GrameneiAT4G30440.1; AT4G30440.1; AT4G30440
    KEGGiath:AT4G30440

    Similar proteinsi

    Entry informationi

    Entry nameiGAE1_ARATH
    AccessioniPrimary (citable) accession number: Q9M0B6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 109 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health