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Protein

UDP-glucuronate 4-epimerase 1

Gene

GAE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.1 Publication

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Enzyme regulationi

Inhibited by UDP-Xylose.

Kineticsi

Equilibrium between UDP-glucuronate and UDP-D-galacturonate established at 1:1.3. No activity with UDP-Galactose, UDP-Glucose or UDP-Xylose.

  1. KM=0.19 mM for UDP-glucuronate1 Publication

    pH dependencei

    Optimum pH is 7.6. Active from pH 6 to 8.9.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei239 – 2391Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi89 – 12032NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    • UDP-glucuronate 4-epimerase activity Source: TAIR

    GO - Biological processi

    • defense response to fungus Source: TAIR
    • defense response to Gram-negative bacterium Source: TAIR
    • galacturonate biosynthetic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi5.1.3.6. 399.
    SABIO-RKQ9M0B6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronate 4-epimerase 1 (EC:5.1.3.6)
    Alternative name(s):
    UDP-glucuronic acid epimerase 1
    Short name:
    AtUGlcAE3
    Gene namesi
    Name:GAE1
    Synonyms:UGlcAE3
    Ordered Locus Names:At4g30440
    ORF Names:F17I23.220
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G30440.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621HelicalSequence analysisAdd
    BLAST
    Transmembranei87 – 10721HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • endosome Source: TAIR
    • Golgi apparatus Source: TAIR
    • Golgi cisterna membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW
    • trans-Golgi network Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 429429UDP-glucuronate 4-epimerase 1PRO_0000292596Add
    BLAST

    Proteomic databases

    PaxDbiQ9M0B6.
    PRIDEiQ9M0B6.

    PTM databases

    iPTMnetiQ9M0B6.
    SwissPalmiQ9M0B6.

    Expressioni

    Tissue specificityi

    In root stele, leaves, siliques, flowers, pollen and stems.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9M0B6. baseline and differential.
    GenevisibleiQ9M0B6. AT.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G30440.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M0B6.
    SMRiQ9M0B6. Positions 88-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi213 – 2164Poly-Ser

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IU3F. Eukaryota.
    COG0451. LUCA.
    HOGENOMiHOG000168000.
    InParanoidiQ9M0B6.
    KOiK08679.
    OMAiQVRNSAQ.
    PhylomeDBiQ9M0B6.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR008089. Nuc_sugar_epim.
    [Graphical view]
    PfamiPF16363. GDP_Man_Dehyd. 1 hit.
    [Graphical view]
    PRINTSiPR01713. NUCEPIMERASE.
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9M0B6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSIEDELFP STPGKFKIDR SNRQLHRCFA STSTMFLWAL FLIALTASYL
    60 70 80 90 100
    SFQSFVDSGS RYLTASWGGI QWEKQVRTSA QIHRSGGISV LVTGATGFVG
    110 120 130 140 150
    SHVSLALRKR GDGVVGLDNF NNYYDPSLKR ARRSLLSSRG IFVVEGDLND
    160 170 180 190 200
    AKLLAKLFDV VAFTHVMHLA AQAGVRYALE NPQSYVHSNI AGLVNLLEIC
    210 220 230 240 250
    KAANPQPAIV WASSSSVYGL NEKVPFSESD RTDQPASLYA ATKKAGEEIT
    260 270 280 290 300
    HTYNHIYGLA ITGLRFFTVY GPWGRPDMAY FSFTRNILQG KPITIYRGKN
    310 320 330 340 350
    RVDLARDFTY IDDIVKGCLG SLDSSGKSTG SGGKKRGAAP YRIFNLGNTS
    360 370 380 390 400
    PVTVPILVDI LEKHLKVKAK RNFVEMPGNG DVPFTHANIS SARNEFGYKP
    410 420
    TTDLETGLKK FVRWYLSYYG YNTKAKLVH
    Length:429
    Mass (Da):47,458
    Last modified:October 1, 2000 - v1
    Checksum:i1FDD14BC3FDABCD4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY661562 Genomic DNA. Translation: AAT77233.1.
    AL161577 Genomic DNA. Translation: CAB79762.1.
    CP002687 Genomic DNA. Translation: AEE85765.1.
    AY056303 mRNA. Translation: AAL07152.1.
    AY099855 mRNA. Translation: AAM20706.1.
    BT000308 mRNA. Translation: AAN15627.1.
    AY085505 mRNA. Translation: AAM62729.1.
    PIRiA85356.
    RefSeqiNP_194773.1. NM_119190.3.
    UniGeneiAt.20969.
    At.67043.

    Genome annotation databases

    EnsemblPlantsiAT4G30440.1; AT4G30440.1; AT4G30440.
    GeneIDi829167.
    GrameneiAT4G30440.1; AT4G30440.1; AT4G30440.
    KEGGiath:AT4G30440.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY661562 Genomic DNA. Translation: AAT77233.1.
    AL161577 Genomic DNA. Translation: CAB79762.1.
    CP002687 Genomic DNA. Translation: AEE85765.1.
    AY056303 mRNA. Translation: AAL07152.1.
    AY099855 mRNA. Translation: AAM20706.1.
    BT000308 mRNA. Translation: AAN15627.1.
    AY085505 mRNA. Translation: AAM62729.1.
    PIRiA85356.
    RefSeqiNP_194773.1. NM_119190.3.
    UniGeneiAt.20969.
    At.67043.

    3D structure databases

    ProteinModelPortaliQ9M0B6.
    SMRiQ9M0B6. Positions 88-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G30440.1.

    PTM databases

    iPTMnetiQ9M0B6.
    SwissPalmiQ9M0B6.

    Proteomic databases

    PaxDbiQ9M0B6.
    PRIDEiQ9M0B6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G30440.1; AT4G30440.1; AT4G30440.
    GeneIDi829167.
    GrameneiAT4G30440.1; AT4G30440.1; AT4G30440.
    KEGGiath:AT4G30440.

    Organism-specific databases

    TAIRiAT4G30440.

    Phylogenomic databases

    eggNOGiENOG410IU3F. Eukaryota.
    COG0451. LUCA.
    HOGENOMiHOG000168000.
    InParanoidiQ9M0B6.
    KOiK08679.
    OMAiQVRNSAQ.
    PhylomeDBiQ9M0B6.

    Enzyme and pathway databases

    BRENDAi5.1.3.6. 399.
    SABIO-RKQ9M0B6.

    Miscellaneous databases

    PROiQ9M0B6.

    Gene expression databases

    ExpressionAtlasiQ9M0B6. baseline and differential.
    GenevisibleiQ9M0B6. AT.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR008089. Nuc_sugar_epim.
    [Graphical view]
    PfamiPF16363. GDP_Man_Dehyd. 1 hit.
    [Graphical view]
    PRINTSiPR01713. NUCEPIMERASE.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The biosynthesis of D-galacturonate in plants. Functional cloning and characterization of a membrane-anchored UDP-D-glucuronate 4-epimerase from Arabidopsis."
      Moelhoej M., Verma R., Reiter W.-D.
      Plant Physiol. 135:1221-1230(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Molecular genetics of nucleotide sugar interconversion pathways in plants."
      Reiter W.-D., Vanzin G.F.
      Plant Mol. Biol. 47:95-113(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, NOMENCLATURE.
    7. "Identification and characterization of a UDP-D-glucuronate 4-epimerase in Arabidopsis."
      Usadel B., Schlueter U., Moelhoej M., Gipmans M., Verma R., Kossmann J., Reiter W.-D., Pauly M.
      FEBS Lett. 569:327-331(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "The biosynthesis of UDP-galacturonic acid in plants. Functional cloning and characterization of Arabidopsis UDP-D-glucuronic acid 4-epimerase."
      Gu X., Bar-Peled M.
      Plant Physiol. 136:4256-4264(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, TISSUE SPECIFICITY, NOMENCLATURE.
    9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGAE1_ARATH
    AccessioniPrimary (citable) accession number: Q9M0B6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: October 1, 2000
    Last modified: May 11, 2016
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.