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Protein

Serine carboxypeptidase 24

Gene

SCPL24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Active serine carboxypeptidase with broad substrate preference, including basic and hydrophilic groups. Processes a protein involved in an early event in the brassinosteroid signaling pathway.2 Publications

Miscellaneous

Was isolated as a suppressor of bri1 mutant phenotype. The serine carboxypeptidase activity is necessary for suppression of bri1 mutant phenotype.

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Enzyme regulationi

Completely inhibited by phenylmethylsulfonyl fluoride (PMSF) and partially by leupeptin.1 Publication

pH dependencei

Optimum pH is 5.5.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei181By similarity1
Active sitei386By similarity1
Active sitei438By similarity1

GO - Molecular functioni

  • serine-type carboxypeptidase activity Source: TAIR

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: TAIR
  • proteolysis Source: TAIR
  • proteolysis involved in cellular protein catabolic process Source: GO_Central

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-ATH-2132295 MHC class II antigen presentation
R-ATH-6798695 Neutrophil degranulation

Protein family/group databases

ESTHERiarath-AT4g30610 Carboxypeptidase_S10
MEROPSiS10.015

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 24 (EC:3.4.16.6)
Alternative name(s):
Bri1 suppressor 1
Carboxypeptidase D
Serine carboxypeptidase II
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase II chain A
Alternative name(s):
Serine carboxypeptidase II chain B
Gene namesi
Name:SCPL24
Synonyms:BRS1
Ordered Locus Names:At4g30610
ORF Names:F17I23.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30610
TAIRilocus:2118706 AT4G30610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181S → F: Loss of activity. 1 Publication1
Mutagenesisi438H → A: Loss of activity. Decrease in A and B chains cleavage efficiency. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000430625 – 286Serine carboxypeptidase 24 chain ABy similarityAdd BLAST262
PropeptideiPRO_0000004307287 – 316Linker peptideBy similarityAdd BLAST30
ChainiPRO_0000004308317 – 465Serine carboxypeptidase 24 chain BBy similarityAdd BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi88 ↔ 349Interchain (between A and B chains)By similarity
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi249 ↔ 260By similarity
Glycosylationi250N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi285 ↔ 317Interchain (between A and B chains)By similarity
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi338N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9M099
PRIDEiQ9M099

Expressioni

Tissue specificityi

Expressed in shoots, leaves, cauline leaves, siliques and flowers. Expressed a low levels in roots and stems.2 Publications

Gene expression databases

ExpressionAtlasiQ9M099 baseline and differential
GenevisibleiQ9M099 AT

Interactioni

Subunit structurei

Heterodimer.Curated

Protein-protein interaction databases

STRINGi3702.AT4G30610.1

Structurei

3D structure databases

ProteinModelPortaliQ9M099
SMRiQ9M099
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282 Eukaryota
COG2939 LUCA
HOGENOMiHOG000198295
InParanoidiQ9M099
KOiK16297
OMAiVRMKNTL
OrthoDBiEOG09360880
PhylomeDBiQ9M099

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR001563 Peptidase_S10
IPR033124 Ser_caboxypep_his_AS
IPR018202 Ser_caboxypep_ser_AS
PANTHERiPTHR11802 PTHR11802, 1 hit
PfamiView protein in Pfam
PF00450 Peptidase_S10, 1 hit
PRINTSiPR00724 CRBOXYPTASEC
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00560 CARBOXYPEPT_SER_HIS, 1 hit
PS00131 CARBOXYPEPT_SER_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTHFIFLL LVALLSTTFP SSSSSREQEK DRIKALPGQP KVAFSQYSGY
60 70 80 90 100
VNVNQSHGRA LFYWLTESSS PSPHTKPLLL WLNGGPGCSS IAYGASEEIG
110 120 130 140 150
PFRINKTGSN LYLNKFAWNK DANLLFLESP AGVGYSYTNT SSDLKDSGDE
160 170 180 190 200
RTAQDNLIFL IKWLSRFPQY KYRDFYIAGE SYAGHYVPQL AKKINDYNKA
210 220 230 240 250
FSKPIINLKG FLVGNAVTDN QYDSIGTVTY WWTHAIISDK SYKSILKYCN
260 270 280 290 300
FTVERVSDDC DNAVNYAMNH EFGDIDQYSI YTPTCVAAQQ KKNTTGFFVR
310 320 330 340 350
MKNTLLRRRL VSGYDPCTES YAEKYFNRPD VQRAMHANVT GIRYKWTACS
360 370 380 390 400
DVLIKTWKDS DKTMLPIYKE LAASGLRIWI FSGDTDSVVP VTATRFSLSH
410 420 430 440 450
LNLPVKTRWY PWYTDNQVGG WTEVYKGLTF ATVRGAGHEV PLFEPKRALI
460
LFRSFLAGKE LPRSY
Length:465
Mass (Da):52,845
Last modified:October 1, 2000 - v1
Checksum:i6403510C34110033
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107T → N in AAK44013 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161577 Genomic DNA Translation: CAB79779.1
CP002687 Genomic DNA Translation: AEE85786.1
BT002334 mRNA Translation: AAN86167.1
AF370198 mRNA Translation: AAK44013.1
PIRiB85358
RefSeqiNP_194790.1, NM_119207.3
UniGeneiAt.31819
At.4582

Genome annotation databases

EnsemblPlantsiAT4G30610.1; AT4G30610.1; AT4G30610
GeneIDi829184
GrameneiAT4G30610.1; AT4G30610.1; AT4G30610
KEGGiath:AT4G30610

Similar proteinsi

Entry informationi

Entry nameiSCP24_ARATH
AccessioniPrimary (citable) accession number: Q9M099
Secondary accession number(s): Q94K84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health