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Protein

Aspartate--tRNA ligase 2, cytoplasmic

Gene

IBI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (By similarity). Involved in the perception of beta-aminobutyric acid (BABA) and required for BABA priming effect in disease resistance (PubMed:24776930).By similarity1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei286AspartateBy similarity1
Binding sitei330AspartateBy similarity1
Metal bindingi481Magnesium 2By similarity1
Metal bindingi481Magnesium 3By similarity1
Binding sitei481ATPBy similarity1
Metal bindingi484Magnesium 2By similarity1
Binding sitei484AspartateBy similarity1
Binding sitei488AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi110 – 195OBSequence analysisAdd BLAST86
Nucleotide bindingi330 – 332ATPBy similarity3
Nucleotide bindingi338 – 340ATPBy similarity3
Nucleotide bindingi529 – 532ATPBy similarity4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • aspartyl-tRNA aminoacylation Source: InterPro
  • defense response to fungus Source: TAIR
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, DNA-binding, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase 2, cytoplasmicCurated (EC:6.1.1.12Curated)
Alternative name(s):
Aspartyl-tRNA synthetaseCurated
Short name:
AspRSCurated
Protein IMPAIRED IN BABA-INDUCED DISEASE IMMUNITY 11 Publication
Gene namesi
Name:IBI11 Publication
Ordered Locus Names:At4g31180Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G31180
TAIRilocus:2126306 AT4G31180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004335602 – 558Aspartate--tRNA ligase 2, cytoplasmicAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9M084
PRIDEiQ9M084

PTM databases

iPTMnetiQ9M084

Expressioni

Gene expression databases

ExpressionAtlasiQ9M084 baseline and differential
GenevisibleiQ9M084 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G31180.1

Structurei

3D structure databases

ProteinModelPortaliQ9M084
SMRiQ9M084
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni308 – 311AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0556 Eukaryota
COG0017 LUCA
HOGENOMiHOG000226032
KOiK22503
OMAiWVHEIRD
OrthoDBiEOG09360866
PhylomeDBiQ9M084

Family and domain databases

HAMAPiMF_02075 Asp_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004523 Asp-tRNA_synthase_2
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR43450 PTHR43450, 1 hit
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00458 aspS_nondisc, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSESEIPPL SSSTAAAEES GEKTSKKAAK KEAAKLEKLR RRQEQEEATR
60 70 80 90 100
RTASISLEEN DEFSNNYGDV TLTELQSSAD PKAGKWIEAV EGKEWTDVSD
110 120 130 140 150
LVEEMLESEV LIRGRVHTNR PTSNKLGFVV LRESGSTVQC VVSQSEKTKV
160 170 180 190 200
GANMVKYLKQ LSRESFVDVI GVVTLPKEPL TGTTQQVEIQ VRKVYCINKS
210 220 230 240 250
LAKLPLSVED AARSEADIEA SLQTPSPAAR VNQDTRLNYR VLDLRTPANQ
260 270 280 290 300
AIFQLQYEVE YAFREKLRFK NFVGIHTPKL MAGSSEGGSA VFRLEYKGQP
310 320 330 340 350
ACLAQSPQLH KQMAICGDLR RVFEVGPVFR AEDSFTHRHL CEFVGLDVEM
360 370 380 390 400
EIRKHYSEIM DLVDELFVFI FTSLNERCKK ELQAVGKQYP FEPLKFLPKT
410 420 430 440 450
LRLTFEEGVQ MLKEAGVEVD PLGDLNTESE RKLGQLVLEK YNTEFYILHR
460 470 480 490 500
YPKAVRPFYT MTCADNPLYS NSFDVFIRGE EIISGAQRVH IPEVLEQRAG
510 520 530 540 550
ECGIDVKTIS TYIDSFRYGA PLHGGFGVGL ERVVMLFCAL NNIRKTSLFP

RDPQRLSP
Length:558
Mass (Da):62,918
Last modified:October 1, 2000 - v1
Checksum:i598518C65AD9A4B3
GO

Sequence cautioni

The sequence AAL61938 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161578 Genomic DNA Translation: CAB79836.1
CP002687 Genomic DNA Translation: AEE85872.1
CP002687 Genomic DNA Translation: AEE85873.1
AK317607 mRNA Translation: BAH20270.1
AY072331 mRNA Translation: AAL61938.1 Sequence problems.
BT002567 mRNA Translation: AAO00927.1
PIRiT10672
RefSeqiNP_194847.3, NM_119268.4
NP_849558.1, NM_179227.3
UniGeneiAt.4593

Genome annotation databases

EnsemblPlantsiAT4G31180.1; AT4G31180.1; AT4G31180
AT4G31180.2; AT4G31180.2; AT4G31180
GeneIDi829246
GrameneiAT4G31180.1; AT4G31180.1; AT4G31180
AT4G31180.2; AT4G31180.2; AT4G31180
KEGGiath:AT4G31180

Similar proteinsi

Entry informationi

Entry nameiSYDC2_ARATH
AccessioniPrimary (citable) accession number: Q9M084
Secondary accession number(s): Q8VYA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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