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Protein

ATP-dependent 6-phosphofructokinase 6

Gene

PFK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 6, cytosolic (FAB6), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATP; via amide nitrogenUniRule annotation1
Metal bindingi191Magnesium; catalyticUniRule annotation1
Sitei192Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei221Proton acceptorUniRule annotation1
Binding sitei320SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 166ATPUniRule annotation2
Nucleotide bindingi190 – 193ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G32840-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 6UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 6UniRule annotation
Short name:
Phosphofructokinase 6UniRule annotation
Alternative name(s):
Phosphohexokinase 6UniRule annotation
Gene namesi
Name:PFK6UniRule annotation
Ordered Locus Names:At4g32840
ORF Names:T16I18.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G32840.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: May be associated with the outer membrane of mitochondria.

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003307732 – 462ATP-dependent 6-phosphofructokinase 6Add BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei71PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9M076.
PRIDEiQ9M076.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevisibleiQ9M076. AT.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT4G32840.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M076.
SMRiQ9M076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 221Substrate bindingUniRule annotation3
Regioni264 – 266Substrate bindingUniRule annotation3
Regioni374 – 377Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiQ9M076.
KOiK00850.
OMAiAIYKEIR.
OrthoDBiEOG093609PL.
PhylomeDBiQ9M076.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNGVDEQI KLVEGPAGYV LEDVPHLSDY ILDLPTYPNP LQSNAAYSVV
60 70 80 90 100
RQYFVDEDDT VQEKIVVHKD SPRGTHFRRA GPRQKVYFKP SDVRACIVTC
110 120 130 140 150
GGLCPGLNTV IREIVCGLHF MYGVTEVIGV DCGFRGFYSK NTVALTPKTV
160 170 180 190 200
SDIHKRGGTI LGTSRGGHDT SKIVDNIQDR EINQVYIIGG DGTQKGANAI
210 220 230 240 250
YKEIRRRGLK VAVAGIPKTI DNDIPVIDKS FGFDTAVEEA QRAINAAHVE
260 270 280 290 300
ATSVENGIGI VKLMGRYSGF IAMYATLASR DVDCCLIPES PFYLEGKGGL
310 320 330 340 350
YEFIAKRLRE NGHMVIVIAE GAGQDLVAES IEQQDASGNK LLKDVGLWMS
360 370 380 390 400
LKIKEYFAKH NVMDITLKYI DPTYMIRAIP ANASDNVYST LLAQSAVHGA
410 420 430 440 450
MAGYTGFVSG LVNGRHTYIP FNRITERQNK VVITDRMWAR MLSSTNQPSF
460
MNPPKGTTEF TD
Length:462
Mass (Da):50,788
Last modified:October 1, 2000 - v1
Checksum:i283E7758C5DB8C24
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti282V → A in AAM65566 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161582 Genomic DNA. Translation: CAB80001.1.
CP002687 Genomic DNA. Translation: AEE86125.1.
BT006001 mRNA. Translation: AAO64936.1.
AK227471 mRNA. Translation: BAE99473.1.
AY088020 mRNA. Translation: AAM65566.1.
PIRiT10691.
RefSeqiNP_195010.1. NM_119437.3.
UniGeneiAt.31631.

Genome annotation databases

EnsemblPlantsiAT4G32840.1; AT4G32840.1; AT4G32840.
GeneIDi829420.
GrameneiAT4G32840.1; AT4G32840.1; AT4G32840.
KEGGiath:AT4G32840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161582 Genomic DNA. Translation: CAB80001.1.
CP002687 Genomic DNA. Translation: AEE86125.1.
BT006001 mRNA. Translation: AAO64936.1.
AK227471 mRNA. Translation: BAE99473.1.
AY088020 mRNA. Translation: AAM65566.1.
PIRiT10691.
RefSeqiNP_195010.1. NM_119437.3.
UniGeneiAt.31631.

3D structure databases

ProteinModelPortaliQ9M076.
SMRiQ9M076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G32840.1.

Proteomic databases

PaxDbiQ9M076.
PRIDEiQ9M076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G32840.1; AT4G32840.1; AT4G32840.
GeneIDi829420.
GrameneiAT4G32840.1; AT4G32840.1; AT4G32840.
KEGGiath:AT4G32840.

Organism-specific databases

TAIRiAT4G32840.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiQ9M076.
KOiK00850.
OMAiAIYKEIR.
OrthoDBiEOG093609PL.
PhylomeDBiQ9M076.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciARA:AT4G32840-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.

Miscellaneous databases

PROiQ9M076.

Gene expression databases

GenevisibleiQ9M076. AT.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA6_ARATH
AccessioniPrimary (citable) accession number: Q9M076
Secondary accession number(s): Q8LA55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.