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Protein

Glucan endo-1,3-beta-glucosidase 7

Gene

At4g34480

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641NucleophileBy similarity
Active sitei327 – 3271Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT4G34480-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 7 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 7
Short name:
(1->3)-beta-glucanase 7
Beta-1,3-endoglucanase 7
Short name:
Beta-1,3-glucanase 7
Gene namesi
Ordered Locus Names:At4g34480
ORF Names:T4L20.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34480.

Subcellular locationi

Secreted 1 Publication. Secretedcell wall 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 504482Glucan endo-1,3-beta-glucosidase 7PRO_0000042684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi365 ↔ 427By similarity

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9M069.
PRIDEiQ9M069.

Expressioni

Gene expression databases

GenevestigatoriQ9M069.

Structurei

3D structure databases

ProteinModelPortaliQ9M069.
SMRiQ9M069. Positions 26-345, 351-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi449 – 46012Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG309748.
HOGENOMiHOG000238220.
InParanoidiQ9M069.
OMAiCCFVAAS.
PhylomeDBiQ9M069.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSISIYFL LIFLSHFPSS HAEPFIGVNY GQVADNLPPP SETVKLLQST
60 70 80 90 100
SIQKVRLYGA DPAIIKALAG TGVGIVIGAA NGDVPSLASD PNAATQWINS
110 120 130 140 150
NVLPFYPASK IMLITVGNEI LMSNDPNLVN QLLPAMQNVQ KALEAVSLGG
160 170 180 190 200
KIKVSTVNSM TVLGSSDPPS SGSFAAGYQT GLKGILQFLS DTGSPFAINP
210 220 230 240 250
YPFFAYQSDP RPETLAFCLF EPNAGRVDSK TGIKYTNMFD AQVDAVHSAL
260 270 280 290 300
KSMGFEKVEI VVAETGWASR GDANEVGASV DNAKAYNGNL IAHLRSMVGT
310 320 330 340 350
PLMPGKPVDT YIFALYDENL KPGPSSERAF GLFKTDLSMV YDVGLAKSSS
360 370 380 390 400
SSQTPSGKVT SSGWCVPKKG ATNEELQASL DWACGHGIDC GAIQPGGACF
410 420 430 440 450
EPNNVVSHAA YAMNMYFQKS PKQPTDCDFS KTATVTSQNP SYNNCVYPGG
460 470 480 490 500
GGGGGGGGGG SKAVMNKYVS SDKVEKKNGA TEPKVSSSLS FLLIFLSLIF

HVYM
Length:504
Mass (Da):53,121
Last modified:September 21, 2009 - v2
Checksum:i77FF26A6AF36C167
GO

Sequence cautioni

The sequence CAA18827.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80165.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023094 Genomic DNA. Translation: CAA18827.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80165.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86384.1.
PIRiD85406.
T05268.
RefSeqiNP_195174.6. NM_119613.7.
UniGeneiAt.48072.

Genome annotation databases

EnsemblPlantsiAT4G34480.1; AT4G34480.1; AT4G34480.
GeneIDi829599.
KEGGiath:AT4G34480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023094 Genomic DNA. Translation: CAA18827.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80165.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86384.1.
PIRiD85406.
T05268.
RefSeqiNP_195174.6. NM_119613.7.
UniGeneiAt.48072.

3D structure databases

ProteinModelPortaliQ9M069.
SMRiQ9M069. Positions 26-345, 351-450.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ9M069.
PRIDEiQ9M069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34480.1; AT4G34480.1; AT4G34480.
GeneIDi829599.
KEGGiath:AT4G34480.

Organism-specific databases

TAIRiAT4G34480.

Phylogenomic databases

eggNOGiNOG309748.
HOGENOMiHOG000238220.
InParanoidiQ9M069.
OMAiCCFVAAS.
PhylomeDBiQ9M069.

Enzyme and pathway databases

BioCyciARA:AT4G34480-MONOMER.

Gene expression databases

GenevestigatoriQ9M069.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia1 Publication.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
    Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
    J. Biol. Chem. 272:15841-15848(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-38, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiE137_ARATH
AccessioniPrimary (citable) accession number: Q9M069
Secondary accession number(s): O65675, P80829
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2005
Last sequence update: September 21, 2009
Last modified: January 6, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.