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Q9LZX6

- DAPA1_ARATH

UniProt

Q9LZX6 - DAPA1_ARATH

Protein

4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic

Gene

DHDPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (21 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).By similarity

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei107 – 1071Part of a proton relay during catalysisBy similarity
    Binding sitei108 – 1081PyruvateBy similarity
    Sitei170 – 1701Part of a proton relay during catalysisBy similarity
    Active sitei194 – 1941Proton donor/acceptorBy similarity
    Active sitei222 – 2221Schiff-base intermediate with substrateBy similarity
    Binding sitei261 – 2611Pyruvate; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: TAIR

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-KW
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciARA:AT3G60880-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic (EC:4.3.3.7)
    Short name:
    HTPA synthase 1
    Gene namesi
    Name:DHDPS1
    Synonyms:DHDPS, DHPS1
    Ordered Locus Names:At3g60880
    ORF Names:T4C21_290
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G60880.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939ChloroplastSequence AnalysisAdd
    BLAST
    Chaini40 – 3653264-hydroxy-tetrahydrodipicolinate synthase 1, chloroplasticPRO_0000007197Add
    BLAST

    Proteomic databases

    PaxDbiQ9LZX6.
    PRIDEiQ9LZX6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LZX6.

    Interactioni

    Protein-protein interaction databases

    BioGridi10573. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LZX6.
    SMRiQ9LZX6. Positions 69-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    InParanoidiQ9LZX6.
    KOiK01714.
    OMAiQQRASCG.
    PhylomeDBiQ9LZX6.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LZX6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSALKNYGLI SIDSALHFPR SNQLQSYKRR NAKWVSPIAA VVPNFHLPMR    50
    SLEDKNRTNT DDIRSLRVIT AIKTPYLPDG RFDLQAYDDL VNTQIENGAE 100
    GVIVGGTTGE GQLMSWDEHI MLIGHTVNCF GGRIKVIGNT GSNSTREAIH 150
    ATEQGFAMGM HGALHINPYY GKTSIEGMNA HFQTVLHMGP TIIYNVPGRT 200
    CQDIPPQVIF KLSQNPNMAG VKECVGNNRV EEYTEKGIVV WSGNDDQCHD 250
    SRWDHGATGV ISVTSNLVPG LMRKLMFEGR NSALNAKLLP LMDWLFQEPN 300
    PIGVNTALAQ LGVARPVFRL PYVPLPLSKR IEFVKLVKEI GREHFVGDRD 350
    VQVLDDDDFI LIGRY 365

    Note: May be due to a competing acceptor splice site.

    Length:365
    Mass (Da):40,620
    Last modified:November 21, 2003 - v2
    Checksum:iF07BBAE68F004484
    GO
    Isoform 2 (identifier: Q9LZX6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         30-30: Missing.

    Show »
    Length:364
    Mass (Da):40,464
    Checksum:i73895B17AA79E6EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti286 – 2861A → E in CAB45642. (PubMed:8049377)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei30 – 301Missing in isoform 2. 2 PublicationsVSP_009000

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72971 mRNA. Translation: CAB45642.1.
    AL162295 Genomic DNA. Translation: CAB82692.1.
    CP002686 Genomic DNA. Translation: AEE80120.1.
    CP002686 Genomic DNA. Translation: AEE80121.1.
    AY063943 mRNA. Translation: AAL36299.1.
    AY096442 mRNA. Translation: AAM20082.1.
    AY087718 mRNA. Translation: AAM65255.1.
    X98080 Genomic DNA. Translation: CAA66703.1.
    PIRiS46304.
    T47899.
    RefSeqiNP_191647.1. NM_115952.2. [Q9LZX6-2]
    NP_850730.1. NM_180399.2. [Q9LZX6-1]
    UniGeneiAt.437.

    Genome annotation databases

    EnsemblPlantsiAT3G60880.2; AT3G60880.2; AT3G60880. [Q9LZX6-1]
    GeneIDi825259.
    KEGGiath:AT3G60880.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72971 mRNA. Translation: CAB45642.1 .
    AL162295 Genomic DNA. Translation: CAB82692.1 .
    CP002686 Genomic DNA. Translation: AEE80120.1 .
    CP002686 Genomic DNA. Translation: AEE80121.1 .
    AY063943 mRNA. Translation: AAL36299.1 .
    AY096442 mRNA. Translation: AAM20082.1 .
    AY087718 mRNA. Translation: AAM65255.1 .
    X98080 Genomic DNA. Translation: CAA66703.1 .
    PIRi S46304.
    T47899.
    RefSeqi NP_191647.1. NM_115952.2. [Q9LZX6-2 ]
    NP_850730.1. NM_180399.2. [Q9LZX6-1 ]
    UniGenei At.437.

    3D structure databases

    ProteinModelPortali Q9LZX6.
    SMRi Q9LZX6. Positions 69-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 10573. 1 interaction.

    Proteomic databases

    PaxDbi Q9LZX6.
    PRIDEi Q9LZX6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G60880.2 ; AT3G60880.2 ; AT3G60880 . [Q9LZX6-1 ]
    GeneIDi 825259.
    KEGGi ath:AT3G60880.

    Organism-specific databases

    TAIRi AT3G60880.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    InParanoidi Q9LZX6.
    KOi K01714.
    OMAi QQRASCG.
    PhylomeDBi Q9LZX6.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci ARA:AT3G60880-MONOMER.

    Gene expression databases

    Genevestigatori Q9LZX6.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone."
      Vauterin M., Jacobs M.
      Plant Mol. Biol. 25:545-550(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. Vauterin M.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 1-159 (ISOFORM 2).

    Entry informationi

    Entry nameiDAPA1_ARATH
    AccessioniPrimary (citable) accession number: Q9LZX6
    Secondary accession number(s): O49355, Q8VZQ1, Q9SW58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: November 21, 2003
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3