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Protein

4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic

Gene

DHDPS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).By similarity

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. AK1 (AXX17_At5g12690), Aspartokinase 2, chloroplastic (AK2), Aspartokinase (AXX17_At5g13510), Aspartokinase 1, chloroplastic (AK1), Aspartokinase (CARAB-AK-LYS), Aspartokinase 3, chloroplastic (AK3), Aspartokinase (AXX17_At3g01160), Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic (AKHSDH2), Aspartokinase (AXX17_At3g01160), Aspartokinase (AXX17_At3g01160), Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic (AKHSDH1)
  2. no protein annotated in this organism
  3. 4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic (DHDPS1), 4-hydroxy-tetrahydrodipicolinate synthase 2, chloroplastic (DHDPS2)
  4. 4-hydroxy-tetrahydrodipicolinate reductase 2, chloroplastic (DAPB2), 4-hydroxy-tetrahydrodipicolinate reductase 1, chloroplastic (DAPB1)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Part of a proton relay during catalysisBy similarity1
Binding sitei108PyruvateBy similarity1
Sitei170Part of a proton relay during catalysisBy similarity1
Active sitei194Proton donor/acceptorBy similarity1
Active sitei222Schiff-base intermediate with substrateBy similarity1
Binding sitei261Pyruvate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • 4-hydroxy-tetrahydrodipicolinate synthase Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciARA:AT3G60880-MONOMER
UniPathwayiUPA00034; UER00017

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic (EC:4.3.3.7)
Short name:
HTPA synthase 1
Gene namesi
Name:DHDPS1
Synonyms:DHDPS, DHPS1
Ordered Locus Names:At3g60880
ORF Names:T4C21_290
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G60880
TAIRilocus:2101921 AT3G60880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39ChloroplastSequence analysisAdd BLAST39
ChainiPRO_000000719740 – 3654-hydroxy-tetrahydrodipicolinate synthase 1, chloroplasticAdd BLAST326

Proteomic databases

PaxDbiQ9LZX6
PRIDEiQ9LZX6

Expressioni

Gene expression databases

ExpressionAtlasiQ9LZX6 baseline and differential
GenevisibleiQ9LZX6 AT

Interactioni

Protein-protein interaction databases

BioGridi10573, 1 interactor
STRINGi3702.AT3G60880.2

Structurei

3D structure databases

ProteinModelPortaliQ9LZX6
SMRiQ9LZX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IJ4Q Eukaryota
COG0329 LUCA
HOGENOMiHOG000173604
InParanoidiQ9LZX6
KOiK01714
OMAiGDEMKAM
OrthoDBiEOG09360ENI
PhylomeDBiQ9LZX6

Family and domain databases

CDDicd00950 DHDPS, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00418 DapA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005263 DapA
IPR002220 DapA-like
IPR020625 Schiff_base-form_aldolases_AS
IPR020624 Schiff_base-form_aldolases_CS
PANTHERiPTHR12128 PTHR12128, 1 hit
PfamiView protein in Pfam
PF00701 DHDPS, 1 hit
PRINTSiPR00146 DHPICSNTHASE
SMARTiView protein in SMART
SM01130 DHDPS, 1 hit
TIGRFAMsiTIGR00674 dapA, 1 hit
PROSITEiView protein in PROSITE
PS00665 DHDPS_1, 1 hit
PS00666 DHDPS_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LZX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSALKNYGLI SIDSALHFPR SNQLQSYKRR NAKWVSPIAA VVPNFHLPMR
60 70 80 90 100
SLEDKNRTNT DDIRSLRVIT AIKTPYLPDG RFDLQAYDDL VNTQIENGAE
110 120 130 140 150
GVIVGGTTGE GQLMSWDEHI MLIGHTVNCF GGRIKVIGNT GSNSTREAIH
160 170 180 190 200
ATEQGFAMGM HGALHINPYY GKTSIEGMNA HFQTVLHMGP TIIYNVPGRT
210 220 230 240 250
CQDIPPQVIF KLSQNPNMAG VKECVGNNRV EEYTEKGIVV WSGNDDQCHD
260 270 280 290 300
SRWDHGATGV ISVTSNLVPG LMRKLMFEGR NSALNAKLLP LMDWLFQEPN
310 320 330 340 350
PIGVNTALAQ LGVARPVFRL PYVPLPLSKR IEFVKLVKEI GREHFVGDRD
360
VQVLDDDDFI LIGRY
Note: May be due to a competing acceptor splice site.
Length:365
Mass (Da):40,620
Last modified:November 21, 2003 - v2
Checksum:iF07BBAE68F004484
GO
Isoform 2 (identifier: Q9LZX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     30-30: Missing.

Show »
Length:364
Mass (Da):40,464
Checksum:i73895B17AA79E6EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286A → E in CAB45642 (PubMed:8049377).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00900030Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72971 mRNA Translation: CAB45642.1
AL162295 Genomic DNA Translation: CAB82692.1
CP002686 Genomic DNA Translation: AEE80120.1
CP002686 Genomic DNA Translation: AEE80121.1
AY063943 mRNA Translation: AAL36299.1
AY096442 mRNA Translation: AAM20082.1
AY087718 mRNA Translation: AAM65255.1
X98080 Genomic DNA Translation: CAA66703.1
PIRiS46304
T47899
RefSeqiNP_191647.1, NM_115952.2 [Q9LZX6-2]
NP_850730.1, NM_180399.3 [Q9LZX6-1]
UniGeneiAt.437

Genome annotation databases

EnsemblPlantsiAT3G60880.1; AT3G60880.1; AT3G60880 [Q9LZX6-2]
AT3G60880.2; AT3G60880.2; AT3G60880 [Q9LZX6-1]
GeneIDi825259
GrameneiAT3G60880.1; AT3G60880.1; AT3G60880 [Q9LZX6-2]
AT3G60880.2; AT3G60880.2; AT3G60880 [Q9LZX6-1]
KEGGiath:AT3G60880

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDAPA1_ARATH
AccessioniPrimary (citable) accession number: Q9LZX6
Secondary accession number(s): O49355, Q8VZQ1, Q9SW58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 21, 2003
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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