Reviewed, UniProtKB/Swiss-Prot Q9LZX6 (DAPA1_ARATH)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase 1, chloroplastic
      Short name=DHDPS 1
    EC=4.2.1.52

Gene names

Name:	DHDPS1
Synonyms:	DHDPS, DHPS1
Ordered Locus Names:	At3g60880
ORF Names:	T4C21_290

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LZX6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: May be due to a competing acceptor splice site.

Isoform 2 (identifier: Q9LZX6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     30-30: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Chloroplast Potential
	Chain	40 – 365	326	Dihydrodipicolinate synthase 1, chloroplastic		PRO_0000007197
Sites
	Active site	222	1	Schiff-base intermediate with substrate By similarity
	Binding site	169	1	Pyruvate By similarity
	Site	194	1	Involved in proton transfer during cleavage By similarity
Natural variations
	Alternative sequence	30	1	Missing in isoform 2.		VSP_009000
Experimental info
	Sequence conflict	286	1	A → E in CAB45642. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: F07BBAE68F004484	FASTA	365	40,620
	10 20 30 40 50 60 MSALKNYGLI SIDSALHFPR SNQLQSYKRR NAKWVSPIAA VVPNFHLPMR SLEDKNRTNT 70 80 90 100 110 120 DDIRSLRVIT AIKTPYLPDG RFDLQAYDDL VNTQIENGAE GVIVGGTTGE GQLMSWDEHI 130 140 150 160 170 180 MLIGHTVNCF GGRIKVIGNT GSNSTREAIH ATEQGFAMGM HGALHINPYY GKTSIEGMNA 190 200 210 220 230 240 HFQTVLHMGP TIIYNVPGRT CQDIPPQVIF KLSQNPNMAG VKECVGNNRV EEYTEKGIVV 250 260 270 280 290 300 WSGNDDQCHD SRWDHGATGV ISVTSNLVPG LMRKLMFEGR NSALNAKLLP LMDWLFQEPN 310 320 330 340 350 360 PIGVNTALAQ LGVARPVFRL PYVPLPLSKR IEFVKLVKEI GREHFVGDRD VQVLDDDDFI LIGRY
	Isoform 2. Checksum: 73895B17AA79E6EE	FASTA	364	40,464
	10 20 30 40 50 60 MSALKNYGLI SIDSALHFPR SNQLQSYKRN AKWVSPIAAV VPNFHLPMRS LEDKNRTNTD 70 80 90 100 110 120 DIRSLRVITA IKTPYLPDGR FDLQAYDDLV NTQIENGAEG VIVGGTTGEG QLMSWDEHIM 130 140 150 160 170 180 LIGHTVNCFG GRIKVIGNTG SNSTREAIHA TEQGFAMGMH GALHINPYYG KTSIEGMNAH 190 200 210 220 230 240 FQTVLHMGPT IIYNVPGRTC QDIPPQVIFK LSQNPNMAGV KECVGNNRVE EYTEKGIVVW 250 260 270 280 290 300 SGNDDQCHDS RWDHGATGVI SVTSNLVPGL MRKLMFEGRN SALNAKLLPL MDWLFQEPNP 310 320 330 340 350 360 IGVNTALAQL GVARPVFRLP YVPLPLSKRI EFVKLVKEIG REHFVGDRDV QVLDDDDFIL IGRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a poplar and an Arabidopsis thaliana dihydrodipicolinate synthase cDNA clone." Vauterin M., Jacobs M. Plant Mol. Biol. 25:545-550(1994) [PubMed: 8049377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	Vauterin M. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-159 (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72971 mRNA. Translation: CAB45642.1. AL162295 Genomic DNA. Translation: CAB82692.1. AY063943 mRNA. Translation: AAL36299.1. AY096442 mRNA. Translation: AAM20082.1. AY087718 mRNA. Translation: AAM65255.1. X98080 Genomic DNA. Translation: CAA66703.1.
IPI	IPI00534659. IPI00545708.
PIR	S46304. T47899.
RefSeq	NP_191647.1. NP_850730.1.
UniGene	At.437
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LZX6.
Proteomic databases
PRIDE	Q9LZX6.
Genome annotation databases
GeneID	825259.
GenomeReviews	Gene locus AT3G60880 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.17458.
Organism-specific databases
TAIR	At3g60880.
Phylogenomic databases
OMA	QKGLIEH.
Enzyme and pathway databases
BRENDA	4.2.1.52. 302.
Gene expression databases
ArrayExpress	Q9LZX6.
Genevestigator	Q9LZX6.
GermOnline	AT3G60880. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA1_ARATH

Accession

Primary (citable) accession number: Q9LZX6
Secondary accession number(s): O49355, Q8VZQ1, Q9SW58

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 21, 2003
Last modified:	November 3, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)