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Protein

CBL-interacting serine/threonine-protein kinase 14

Gene

CIPK14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 36ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 14 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.15
SOS2-like protein kinase PKS24
Serine/threonine-protein kinase SR1
Short name:
AtSR1
Gene namesi
Name:CIPK14
Synonyms:PKS24, SnRK3.15, SR1
Ordered Locus Names:At5g01820
ORF Names:T20L15.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G01820.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Targeted to the tonoplast when interacting with CBL2 or CBL3 and to the cell membrane when interacting with CBL8.

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi311N → A: No effect on binding to CBL2. 1 Publication1
Mutagenesisi313F → A: Loss of binding to CBL2. 1 Publication1
Mutagenesisi316I → A: No effect on binding to CBL2. 1 Publication1
Mutagenesisi319S → A or D: No effect on binding to CBL2. 1 Publication1
Mutagenesisi322F → A: Loss of binding to CBL2. 1 Publication1
Mutagenesisi324L → A: No effect on binding to CBL2. 1
Mutagenesisi327L → A: No effect on binding to CBL2. 1 Publication1
Mutagenesisi328F → A: No effect on binding to CBL2. 1 Publication1
Mutagenesisi336R → A: No effect on binding to CBL2. 1
Mutagenesisi339R → A: No effect on binding to CBL2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003372161 – 442CBL-interacting serine/threonine-protein kinase 14Add BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei166PhosphoserineBy similarity1
Modified residuei180PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LZW4.
PRIDEiQ9LZW4.

PTM databases

iPTMnetiQ9LZW4.

Expressioni

Tissue specificityi

Predominant in roots, cauline leaves, and flowers.1 Publication

Developmental stagei

First observed in imbibed seeds. Mostly localized in hypocotyls during germination and in seedlings. In mature plants, confined to vascular tissues of leaves and roots. In flowers, expressed in the vascular bundle of the stamen filament and in the stigma, where the filament joins the pistil.1 Publication

Inductioni

By light in a cytokinin-dependent manner and N(6)-benzylaminopurine (BA). Also induced by sucrose, glucose and fructose.2 Publications

Gene expression databases

GenevisibleiQ9LZW4. AT.

Interactioni

Subunit structurei

Interacts with CBL2,CBL3 and CBL8.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBL2Q8LAS79EBI-307576,EBI-485991

Protein-protein interaction databases

BioGridi17041. 49 interactors.
IntActiQ9LZW4. 13 interactors.
STRINGi3702.AT5G01820.1.

Structurei

Secondary structure

1442
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi312 – 317Combined sources6
Beta strandi322 – 324Combined sources3
Helixi325 – 331Combined sources7
Beta strandi338 – 344Combined sources7
Helixi346 – 359Combined sources14
Beta strandi363 – 368Combined sources6
Beta strandi371 – 376Combined sources6
Helixi377 – 379Combined sources3
Beta strandi381 – 389Combined sources9
Beta strandi391 – 393Combined sources3
Beta strandi395 – 404Combined sources10
Helixi412 – 415Combined sources4
Helixi417 – 423Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZFDX-ray1.20B305-427[»]
ProteinModelPortaliQ9LZW4.
SMRiQ9LZW4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LZW4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 276Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini305 – 329NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni162 – 191Activation loopBy similarityAdd BLAST30
Regioni335 – 365PPIBy similarityAdd BLAST31

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9LZW4.
OMAiEIAIMHR.
OrthoDBiEOG09360AX0.
PhylomeDBiQ9LZW4.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LZW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSDPVEFP PENRRGQLFG KYEVGKLVGC GAFAKVYHGR STATGQSVAI
60 70 80 90 100
KVVSKQRLQK GGLNGNIQRE IAIMHRLRHP SIVRLFEVLA TKSKIFFVME
110 120 130 140 150
FAKGGELFAK VSKGRFCEDL SRRYFQQLIS AVGYCHSRGI FHRDLKPENL
160 170 180 190 200
LLDEKLDLKI SDFGLSALTD QIRPDGLLHT LCGTPAYVAP EVLAKKGYDG
210 220 230 240 250
AKIDIWSCGI ILFVLNAGYL PFNDHNLMVM YRKIYKGEFR IPKWTSPDLR
260 270 280 290 300
RLLTRLLDTN PQTRITIEEI IHDPWFKQGY DDRMSKFHLE DSDMKLPADE
310 320 330 340 350
TDSEMGARRM NAFDIISGSP GFNLSGLFGD ARKYDRVERF VSAWTAERVV
360 370 380 390 400
ERLEEIVSAE NLTVAKKETW GMKIEGQKGN FAMVVEINQL TDELVMIEVR
410 420 430 440
KRQRAAASGR DLWTDTLRPF FVELVHESDQ TDPEPTQVHT TS
Length:442
Mass (Da):50,298
Last modified:October 1, 2000 - v1
Checksum:i319532FEFB7244C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295669 mRNA. Translation: AAK16689.1.
AB035147 mRNA. Translation: BAB11737.1.
AL162351 Genomic DNA. Translation: CAB82752.1.
CP002688 Genomic DNA. Translation: AED90397.1.
AF360189 mRNA. Translation: AAK25899.1.
AY142684 mRNA. Translation: AAN13222.1.
PIRiT48203.
RefSeqiNP_195802.1. NM_120260.3.
UniGeneiAt.23738.

Genome annotation databases

EnsemblPlantsiAT5G01820.1; AT5G01820.1; AT5G01820.
GeneIDi831765.
GrameneiAT5G01820.1; AT5G01820.1; AT5G01820.
KEGGiath:AT5G01820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295669 mRNA. Translation: AAK16689.1.
AB035147 mRNA. Translation: BAB11737.1.
AL162351 Genomic DNA. Translation: CAB82752.1.
CP002688 Genomic DNA. Translation: AED90397.1.
AF360189 mRNA. Translation: AAK25899.1.
AY142684 mRNA. Translation: AAN13222.1.
PIRiT48203.
RefSeqiNP_195802.1. NM_120260.3.
UniGeneiAt.23738.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZFDX-ray1.20B305-427[»]
ProteinModelPortaliQ9LZW4.
SMRiQ9LZW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17041. 49 interactors.
IntActiQ9LZW4. 13 interactors.
STRINGi3702.AT5G01820.1.

PTM databases

iPTMnetiQ9LZW4.

Proteomic databases

PaxDbiQ9LZW4.
PRIDEiQ9LZW4.

Protocols and materials databases

DNASUi831765.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G01820.1; AT5G01820.1; AT5G01820.
GeneIDi831765.
GrameneiAT5G01820.1; AT5G01820.1; AT5G01820.
KEGGiath:AT5G01820.

Organism-specific databases

TAIRiAT5G01820.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9LZW4.
OMAiEIAIMHR.
OrthoDBiEOG09360AX0.
PhylomeDBiQ9LZW4.

Miscellaneous databases

EvolutionaryTraceiQ9LZW4.
PROiQ9LZW4.

Gene expression databases

GenevisibleiQ9LZW4. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPKE_ARATH
AccessioniPrimary (citable) accession number: Q9LZW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.