Q9LZS3 (GLGB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic
Short name=AtSBE II-2
EC=2.4.1.18

Alternative name(s):
Branching enzyme 2
Short name=AtBE2
Starch-branching enzyme 2-2

Gene names

Name:	SBE2.2
Synonyms:	BE2
Ordered Locus Names:	At5g03650
ORF Names:	F17C15.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Ref.7
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; starch biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast stroma. Plastid › amyloplast By similarity Ref.6 Ref.9 Ref.11 Ref.12.
Tissue specificity	Expressed in seedlings, roots, stems, leaves, inflorescences, seeds and flowers. Ref.4 Ref.8
Induction	Induced by light when associated with glucose, fructose or sucrose treatment. Induction by glucose is mediated by the transcription factor ABI4. Ref.3 Ref.10
Disruption phenotype	Modified starch composition. This phenotype is enhanced when associated with SBE2.1 and SBE3 disruptions. Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Cellular component	Amyloplast Chloroplast Plastid
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amylopectin biosynthetic process Traceable author statement Ref.4. Source: TAIR cellular response to fructose stimulus Inferred from expression pattern. Source: UniProtKB cellular response to glucose stimulus Inferred from expression pattern. Source: UniProtKB cellular response to light stimulus Inferred from expression pattern. Source: UniProtKB cellular response to sucrose stimulus Inferred from expression pattern. Source: UniProtKB glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro starch biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway starch metabolic process Inferred from mutant phenotype Ref.7. Source: TAIR
Cellular_component	amyloplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from direct assay Ref.6PubMed 18633119. Source: TAIR
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from direct assay Ref.7. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Chloroplast By similarity
	Chain	33 – 805	773	1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic		PRO_0000415336
Sites
	Active site	451	1	Nucleophile By similarity
	Active site	506	1	Proton donor By similarity
Experimental info
	Sequence conflict	6 – 7	2	GV → RS in AAB03100. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9LZS3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7CE130BD9C4941D0	FASTA	805	92,591
	10 20 30 40 50 60 MVVIHGVSLT PRFTLPSRPL NTGFNAGNST LSFFFKKHPL SRKIFAGKQS AEFDSSSQAI 70 80 90 100 110 120 SASEKVLVPD NLDDDPRGFS QIFDLESQTM EYTEAVRTED QTMNVVKERG VKPRIVPPPG 130 140 150 160 170 180 DGKKIYEIDP MLRTYNNHLD YRYGQYKRLR EEIDKYEGGL EAFSRGYEKL GFSRSDAGIT 190 200 210 220 230 240 YREWAPGAKA ASLIGDFNNW NSNADIMTRN EFGVWEIFLP NNTDGSPAIP HGSRVKIRMD 250 260 270 280 290 300 TPSGIKDSIP AWIKFSVQAP GEIPFNGIYY DPPEEEKYVF KHPQPKRPKS LRIYEAHVGM 310 320 330 340 350 360 SSTEPMVNTY ANFRDDVLPR IKKLGYNAVQ IMAIQEHSYY ASFGYHVTNF FAPSSRCGTP 370 380 390 400 410 420 EELKSLIDRA HELGLVVLMD IVHSHASKNT LDGLNMFDGT DAHYFHSGPR GYHWMWDSRL 430 440 450 460 470 480 FNYGSWEVLR YLLSNARWWL EEYKFDGFRF DGVTSMMYTH HGLSVGFTGN YTEYFGLETD 490 500 510 520 530 540 VDAVNYLMLV NDMIHGLYPE AITVGEDVSG MPTFCIPVQD GGVGFDYRLH MAIADKWIEM 550 560 570 580 590 600 LKKRDEDWQM GDIIYTLTNR RWSEKCISYA ESHDQALVGD KTIAFWLMDK DMYDFMAVDR 610 620 630 640 650 660 PSTPLIDRGI ALHKMIRLIT MGLGGEGYLN FMGNEFGHPE WIDFPRGEQR LSDGSVIPGN 670 680 690 700 710 720 NFSYDKCRRR FDLGDADYLR YRGLQEFDQA MQHLEENYGF MTSEHQFISR KDEADRVIVF 730 740 750 760 770 780 ERGDLVFVFN FHWTSSYFDY RIGCSKPGKY KIVLDSDDPL FGGFNRLDRK AEYFTYDGLY 790 800 DERPCSFMVY APCRTAVVYA LANHD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Differential accumulation of Arabidopsis thaliana Sbe 2.1 and Sbe 2.2 transcripts in response to light." Khoshnoodi J., Larsson C.-T., Larsson H., Rask L. Plant Sci. 135:183-193(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81, INDUCTION BY LIGHT. Strain: cv. Columbia.
[4]	"Two closely related cDNAs encoding starch branching enzyme from Arabidopsis thaliana." Fisher D.K., Gao M., Kim K.-N., Boyer C.D., Guiltinan M.J. Plant Mol. Biol. 30:97-108(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-805, TISSUE SPECIFICITY. Strain: cv. Columbia. Tissue: Seedling hypocotyl.
[5]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 589-805. Strain: cv. Columbia.
[6]	"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts." Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J. Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation." Dumez S., Wattebled F., Dauvillee D., Delvalle D., Planchot V., Ball S.G., D'Hulst C. Plant Cell 18:2694-2709(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Wassilewskija.
[8]	"Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants." Han Y., Sun F.-J., Rosales-Mendoza S., Korban S.S. Gene 401:123-130(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[9]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: cv. Columbia.
[10]	"The Arabidopsis ABA-INSENSITIVE (ABI) 4 factor acts as a central transcription activator of the expression of its own gene, and for the induction of ABI5 and SBE2.2 genes during sugar signaling." Bossi F., Cordoba E., Dupre P., Mendoza M.S., Roman C.S., Leon P. Plant J. 59:359-374(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY GLUCOSE. Strain: cv. Columbia.
[11]	"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins." Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N. Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY. Strain: cv. Wassilewskija.
[12]	"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering." Olinares P.D., Ponnala L., van Wijk K.J. Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL162506 Genomic DNA. Translation: CAB82930.1. CP002688 Genomic DNA. Translation: AED90637.1. AJ005130 Genomic DNA. Translation: CAA06392.1. U22428 mRNA. Translation: AAB03100.1. AK117729 mRNA. Translation: BAC42378.1.
IPI	IPI00518558.
PIR	S65046. T48392.
RefSeq	NP_195985.3. NM_120446.3.
UniGene	At.24317. At.4765.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ProteinModelPortal	Q9LZS3.
SMR	Q9LZS3. Positions 121-799.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G03650.1-P.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Proteomic databases
PRIDE	Q9LZS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G03650.1; AT5G03650.1; AT5G03650.
GeneID	831769.
KEGG	ath:AT5G03650.
Organism-specific databases
TAIR	At5g03650.
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000175159.
InParanoid	Q9LZS3.
KO	K00700.
OMA	NDANWIE.
PhylomeDB	Q9LZS3.
ProtClustDB	PLN02447.
Enzyme and pathway databases
UniPathway	UPA00152.
Gene expression databases
ArrayExpress	Q9LZS3.
Genevestigator	Q9LZS3.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_ARATH

Accession

Primary (citable) accession number: Q9LZS3
Secondary accession number(s): O81711, Q42531, Q8GYC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program