SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9LZS3

- GLGB2_ARATH

UniProt

Q9LZS3 - GLGB2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic
Gene
SBE2.2, BE2, At5g03650, F17C15.70
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei451 – 4511Nucleophile By similarity
Active sitei506 – 5061Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: TAIR
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. amylopectin biosynthetic process Source: TAIR
  2. cellular response to fructose stimulus Source: UniProtKB
  3. cellular response to glucose stimulus Source: UniProtKB
  4. cellular response to light stimulus Source: UniProtKB
  5. cellular response to sucrose stimulus Source: UniProtKB
  6. glycogen biosynthetic process Source: InterPro
  7. starch biosynthetic process Source: UniProtKB-UniPathway
  8. starch metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT5G03650-MONOMER.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic (EC:2.4.1.18)
Short name:
AtSBE II-2
Alternative name(s):
Branching enzyme 2
Short name:
AtBE2
Starch-branching enzyme 2-2
Gene namesi
Name:SBE2.2
Synonyms:BE2
Ordered Locus Names:At5g03650
ORF Names:F17C15.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G03650.

Subcellular locationi

Plastidchloroplast stroma. Plastidamyloplast By similarity 4 Publications

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Modified starch composition. This phenotype is enhanced when associated with SBE2.1 and SBE3 disruptions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Chloroplast By similarity
Add
BLAST
Chaini33 – 8057731,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic
PRO_0000415336Add
BLAST

Proteomic databases

PRIDEiQ9LZS3.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, inflorescences, seeds and flowers.2 Publications

Inductioni

Induced by light when associated with glucose, fructose or sucrose treatment. Induction by glucose is mediated by the transcription factor ABI4.2 Publications

Gene expression databases

GenevestigatoriQ9LZS3.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi3702.AT5G03650.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LZS3.
SMRiQ9LZS3. Positions 121-799.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000175159.
InParanoidiQ9LZS3.
KOiK00700.
OMAiNRRWSEK.
PhylomeDBiQ9LZS3.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LZS3-1 [UniParc]FASTAAdd to Basket

« Hide

MVVIHGVSLT PRFTLPSRPL NTGFNAGNST LSFFFKKHPL SRKIFAGKQS    50
AEFDSSSQAI SASEKVLVPD NLDDDPRGFS QIFDLESQTM EYTEAVRTED 100
QTMNVVKERG VKPRIVPPPG DGKKIYEIDP MLRTYNNHLD YRYGQYKRLR 150
EEIDKYEGGL EAFSRGYEKL GFSRSDAGIT YREWAPGAKA ASLIGDFNNW 200
NSNADIMTRN EFGVWEIFLP NNTDGSPAIP HGSRVKIRMD TPSGIKDSIP 250
AWIKFSVQAP GEIPFNGIYY DPPEEEKYVF KHPQPKRPKS LRIYEAHVGM 300
SSTEPMVNTY ANFRDDVLPR IKKLGYNAVQ IMAIQEHSYY ASFGYHVTNF 350
FAPSSRCGTP EELKSLIDRA HELGLVVLMD IVHSHASKNT LDGLNMFDGT 400
DAHYFHSGPR GYHWMWDSRL FNYGSWEVLR YLLSNARWWL EEYKFDGFRF 450
DGVTSMMYTH HGLSVGFTGN YTEYFGLETD VDAVNYLMLV NDMIHGLYPE 500
AITVGEDVSG MPTFCIPVQD GGVGFDYRLH MAIADKWIEM LKKRDEDWQM 550
GDIIYTLTNR RWSEKCISYA ESHDQALVGD KTIAFWLMDK DMYDFMAVDR 600
PSTPLIDRGI ALHKMIRLIT MGLGGEGYLN FMGNEFGHPE WIDFPRGEQR 650
LSDGSVIPGN NFSYDKCRRR FDLGDADYLR YRGLQEFDQA MQHLEENYGF 700
MTSEHQFISR KDEADRVIVF ERGDLVFVFN FHWTSSYFDY RIGCSKPGKY 750
KIVLDSDDPL FGGFNRLDRK AEYFTYDGLY DERPCSFMVY APCRTAVVYA 800
LANHD 805
Length:805
Mass (Da):92,591
Last modified:October 1, 2000 - v1
Checksum:i7CE130BD9C4941D0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 72GV → RS in AAB03100. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL162506 Genomic DNA. Translation: CAB82930.1.
CP002688 Genomic DNA. Translation: AED90637.1.
AJ005130 Genomic DNA. Translation: CAA06392.1.
U22428 mRNA. Translation: AAB03100.1.
AK117729 mRNA. Translation: BAC42378.1.
PIRiS65046.
T48392.
RefSeqiNP_195985.3. NM_120446.3.
UniGeneiAt.24317.
At.4765.

Genome annotation databases

EnsemblPlantsiAT5G03650.1; AT5G03650.1; AT5G03650.
GeneIDi831769.
KEGGiath:AT5G03650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL162506 Genomic DNA. Translation: CAB82930.1 .
CP002688 Genomic DNA. Translation: AED90637.1 .
AJ005130 Genomic DNA. Translation: CAA06392.1 .
U22428 mRNA. Translation: AAB03100.1 .
AK117729 mRNA. Translation: BAC42378.1 .
PIRi S65046.
T48392.
RefSeqi NP_195985.3. NM_120446.3.
UniGenei At.24317.
At.4765.

3D structure databases

ProteinModelPortali Q9LZS3.
SMRi Q9LZS3. Positions 121-799.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G03650.1-P.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEi Q9LZS3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G03650.1 ; AT5G03650.1 ; AT5G03650 .
GeneIDi 831769.
KEGGi ath:AT5G03650.

Organism-specific databases

TAIRi AT5G03650.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000175159.
InParanoidi Q9LZS3.
KOi K00700.
OMAi NRRWSEK.
PhylomeDBi Q9LZS3.

Enzyme and pathway databases

UniPathwayi UPA00152 .
BioCyci ARA:AT5G03650-MONOMER.

Miscellaneous databases

PROi Q9LZS3.

Gene expression databases

Genevestigatori Q9LZS3.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Differential accumulation of Arabidopsis thaliana Sbe 2.1 and Sbe 2.2 transcripts in response to light."
    Khoshnoodi J., Larsson C.-T., Larsson H., Rask L.
    Plant Sci. 135:183-193(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81, INDUCTION BY LIGHT.
    Strain: cv. Columbia.
  4. "Two closely related cDNAs encoding starch branching enzyme from Arabidopsis thaliana."
    Fisher D.K., Gao M., Kim K.-N., Boyer C.D., Guiltinan M.J.
    Plant Mol. Biol. 30:97-108(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-805, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Seedling hypocotyl.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 589-805.
    Strain: cv. Columbia.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Mutants of Arabidopsis lacking starch branching enzyme II substitute plastidial starch synthesis by cytoplasmic maltose accumulation."
    Dumez S., Wattebled F., Dauvillee D., Delvalle D., Planchot V., Ball S.G., D'Hulst C.
    Plant Cell 18:2694-2709(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.
  8. "Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants."
    Han Y., Sun F.-J., Rosales-Mendoza S., Korban S.S.
    Gene 401:123-130(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  9. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  10. "The Arabidopsis ABA-INSENSITIVE (ABI) 4 factor acts as a central transcription activator of the expression of its own gene, and for the induction of ABI5 and SBE2.2 genes during sugar signaling."
    Bossi F., Cordoba E., Dupre P., Mendoza M.S., Roman C.S., Leon P.
    Plant J. 59:359-374(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY GLUCOSE.
    Strain: cv. Columbia.
  11. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Wassilewskija.
  12. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGLGB2_ARATH
AccessioniPrimary (citable) accession number: Q9LZS3
Secondary accession number(s): O81711, Q42531, Q8GYC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi