Reviewed, UniProtKB/Swiss-Prot Q9LZJ5 (AB14C_ARATH)

Names and origin

Protein names

Recommended name:
    ABC transporter C family member 14
      Short name=ABC transporter ABCC.14
      Short name=AtABCC14
    EC=3.6.3.44
Alternative name(s):
    Multidrug resistance-associated protein 10
    Glutathione S-conjugate-transporting ATPase 10
    ATP-energized glutathione S-conjugate pump 10

Gene names

Name:	ABCC14
Synonyms:	MRP10, MRP14
Ordered Locus Names:	At3g62700
ORF Names:	F26K9.130

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pump for glutathione S-conjugates By similarity.
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Ubiquitous. Ref.4
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type vacuole Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1539	1539	ABC transporter C family member 14		PRO_0000226081
Regions
	Transmembrane	45 – 65	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	131 – 151	21	Potential
	Transmembrane	165 – 185	21	Potential
	Transmembrane	197 – 217	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	363 – 383	21	Potential
	Transmembrane	437 – 457	21	Potential
	Transmembrane	461 – 481	21	Potential
	Transmembrane	549 – 569	21	Potential
	Transmembrane	976 – 996	21	Potential
	Transmembrane	1015 – 1035	21	Potential
	Transmembrane	1088 – 1108	21	Potential
	Transmembrane	1112 – 1132	21	Potential
	Transmembrane	1209 – 1229	21	Potential
	Transmembrane	1238 – 1258	21	Potential
	Domain	324 – 605	282	ABC transmembrane type-1 1
	Domain	639 – 862	224	ABC transporter 1
	Domain	980 – 1260	281	ABC transmembrane type-1 2
	Domain	1297 – 1531	235	ABC transporter 2
	Nucleotide binding	674 – 681	8	ATP 1 Potential
	Nucleotide binding	1331 – 1338	8	ATP 2 Potential
Amino acid modifications
	Modified residue	894	1	Phosphoserine Ref.6
	Modified residue	897	1	Phosphoserine Ref.6
	Modified residue	922	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9LZJ5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BF953EC320330648	FASTA	1,539	172,137
	10 20 30 40 50 60 MRWLSSTWLS DLSCSSSSVI EPSSSLPAPI QWLRFILLSP CPQRLLSSTV DVLFLLILFF 70 80 90 100 110 120 FAIQKLCSSS SSRTNGEADI TKPLLGRRTR TRTTGLFKTT VVVTIVLSFC SLVLCVSAFF 130 140 150 160 170 180 TTRTKLKLVD TLFWLIHAVT NVVIAVLVLH LKRFASPSHP LTLRIYWVFN FVVTTLFTVS 190 200 210 220 230 240 GILHLLSDDP AAASLRADDV ASFISFPLTA VLLLVSIKGS TGVVVTTSNV TAPAKSNDVV 250 260 270 280 290 300 VEKSENVSLY ASASFISKTF WLWMNPLLRK GYKSPLNLDQ VPTLSPEHRA EKLATLFESK 310 320 330 340 350 360 WPKPQENSRN PVRTTLIRCF WKEIAFTAVL AIIRLSVIYV GPVLIQSFVD FTSGKRSSPS 370 380 390 400 410 420 QGYYLVLILL IAKFVEVLST HQFNFNSQKL GMLIRSTLIT ALYKKGLKLT GSARQNHGVG 430 440 450 460 470 480 QIVNYMAVDA QQLSDMMLQL HAIWLMPLQV AAAIVLLYNT LGPSVVTTVI GLTGIFVFIL 490 500 510 520 530 540 LGTKRNNRYQ FSLMMNRDSR MKATNEMLNY MRVIKFQAWE DHFNERILKF REMEFGWLSK 550 560 570 580 590 600 FLYSIAGNII VLWSTPVLIS ALTFTTAVFL GVKLDAGTVF TTTTIFKILQ EPIRTFPQSM 610 620 630 640 650 660 ISLSQAMISL GRLDAYMMSR ELSEETVERS QGCDGNVAVE IKDGSFSWDD EDDEPAIENI 670 680 690 700 710 720 NFEVKKGELA AIVGTVGSGK SSLLASVLGE MHKLSGKVRV CGTTAYVAQT SWIQNGTVQD 730 740 750 760 770 780 NILFGLPMNR SKYNEVLKVC CLEKDMQIME FGDQTEIGER GINLSGGQKQ RIQLARAVYQ 790 800 810 820 830 840 ESDVYLLDDV FSAVDAHTGS DIFKKCVRGA LKGKTILLVT HQVDFLHNVD RILVMRDGMI 850 860 870 880 890 900 VQSGKYDELV SSGLDFGELV AAHETSMELV EAGSASATAA NVPMASPITQ RSISIESPRQ 910 920 930 940 950 960 PKSPKVHRTT SMESPRVLRT TSMESPRLSE LNDESIKSFL GSNIPEDGSR LIKEEEREVG 970 980 990 1000 1010 1020 QVSFQVYKLY STEAYGWWGM ILVVFFSVAW QASLMASDYW LAYETSAKNE VSFDATVFIR 1030 1040 1050 1060 1070 1080 VYVIIAAVSI VLVCLRAFYV THLGLKTAQI FFKQILNSLV HAPMSFFDTT PSGRILSRAS 1090 1100 1110 1120 1130 1140 TDQTNVDIFI PFMIGLVATM YTTLLSIFIV TCQYAWPTVF FIIPLGWLNI WYRGYYLASS 1150 1160 1170 1180 1190 1200 RELTRLDSIT KAPVIHHFSE SIAGVMTIRA FKKQPMFRQE NVKRVNANLR MDFHNNGSNE 1210 1220 1230 1240 1250 1260 WLGFRLELIG SWVLCISALF MVMLPSNIIK PENVGLSLSY GLSLNGVLFW AIYLSCFIEN 1270 1280 1290 1300 1310 1320 KMVSVERIKQ FTDIPAEAKW EIKESRPPPN WPYKGNIRLE DVKVRYRPNT PLVLKGLTID 1330 1340 1350 1360 1370 1380 IKGGEKIGVV GRTGSGKSTL IQVLFRLVEP SGGKIIIDGI DICTLGLHDL RSRFGIIPQE 1390 1400 1410 1420 1430 1440 PVLFEGTVRS NIDPTEKYSD EEIWKSLERC QLKDVVASKP EKLDSLVADN GENWSVGQRQ 1450 1460 1470 1480 1490 1500 LLCLGRVMLK RSRILFLDEA TASVDSQTDA MIQKIIREDF SDCTIISIAH RIPTVMDCDR 1510 1520 1530 VLVIDAGKAK EYDSPVRLLE RQSLFAALVQ EYALRSAGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[3]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: GENE FAMILY.
[4]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-894; SER-897 AND SER-922, MASS SPECTROMETRY. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL162651 Genomic DNA. Translation: CAB83120.1.
IPI	IPI00543716.
PIR	T48059.
RefSeq	NP_191829.1.
UniGene	At.4827
3D structure databases
HSSP	HSSP built from PDB template 1R0W based on UniProtKB P26361.
ModBase	Search...
Proteomic databases
PRIDE	Q9LZJ5.
ProMEX	Q9LZJ5.
Genome annotation databases
GeneID	825444.
GenomeReviews	Gene locus AT3G62700 in contig BA000014_GR.
KEGG	ath:AT3G62700.
NMPDR	fig|3702.1.peg.17655.
Organism-specific databases
GeneFarm	2284. 252.
TAIR	At3g62700.
Phylogenomic databases
OMA	NSQKLGM.
Enzyme and pathway databases
BRENDA	3.6.3.44. 302.
Gene expression databases
Genevestigator	Q9LZJ5.
GermOnline	AT3G62700. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB14C_ARATH

Accession

Primary (citable) accession number: Q9LZJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)