Reviewed, UniProtKB/Swiss-Prot Q9LZJ3 (XT1_ARATH)

Names and origin

Protein names

Recommended name:
    Xyloglucan 6-xylosyltransferase
      Short name=AtXT1
    EC=2.4.2.39

Gene names

Name:	XT1
Ordered Locus Names:	At3g62720
ORF Names:	F26K9_150

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfer one xylose mainly to the second glucose residue from the non-reducing end. The acceptor should have a minimum of four glucose residues. Ref.3
Catalytic activity	Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-glucose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	root hair elongation Inferred from genetic interaction. Source: TAIR xyloglucan biosynthetic process Inferred from genetic interaction. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-xylosyltransferase activity Inferred from direct assay. Source: TAIR transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro xyloglucan 6-xylosyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Xyloglucan 6-xylosyltransferase		PRO_0000215169
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 40	20	Signal-anchor for type II membrane protein Potential
	Topological domain	41 – 460	420	Lumenal Potential
	Compositional bias	457 – 460	4	Poly-Thr
Amino acid modifications
	Glycosylation	431	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LZJ3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 60227F8D009D9768	FASTA	460	53,382
	10 20 30 40 50 60 MIEKCIGAHR FRRLQRFMRQ GKVTILCLVL TVIVLRGTIG AGKFGTPEKD IEEIREHFFY 70 80 90 100 110 120 TRKRGEPHRV LVEVSSKTTS SEDGGNGGNS YETFDINKLF VDEGDEEKSR DRTNKPYSLG 130 140 150 160 170 180 PKISDWDEQR RDWLKQNPSF PNFVAPNKPR VLLVTGSAPK PCENPVGDHY LLKSIKNKID 190 200 210 220 230 240 YCRIHGIEIF YNMALLDAEM AGFWAKLPLI RKLLLSHPEI EFLWWMDSDA MFTDMVFELP 250 260 270 280 290 300 WERYKDYNLV MHGWNEMVYD QKNWIGLNTG SFLLRNSQWS LDLLDAWAPM GPKGKIREEA 310 320 330 340 350 360 GKVLTRELKD RPAFEADDQS AMVYLLATER EKWGGKVYLE SGYYLHGYWG ILVDRYEEMI 370 380 390 400 410 420 ENHKPGFGDH RWPLVTHFVG CKPCGKFGDY PVERCLRQMD RAFNFGDNQI LQMYGFTHKS 430 440 450 460 LGSRRVKPTR NQTDRPLDAK DEFGLLHPPF KAAKLSTTTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"An Arabidopsis gene encoding an alpha-xylosyltransferase involved in xyloglucan biosynthesis." Faik A., Price N.J., Raikhel N.V., Keegstra K. Proc. Natl. Acad. Sci. U.S.A. 99:7797-7802(2002) [PubMed: 12032363] [Abstract] Cited for: FUNCTION, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL162651 Genomic DNA. Translation: CAB83122.1. AF424587 mRNA. Translation: AAL11581.1. BT002298 mRNA. Translation: AAN73295.1.
IPI	IPI00536037.
PIR	T48061.
RefSeq	NP_001030917.1. NP_191831.1.
UniGene	At.4829
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Proteomic databases
PRIDE	Q9LZJ3.
Genome annotation databases
GeneID	825446.
GenomeReviews	Gene locus AT3G62720 in contig BA000014_GR.
KEGG	ath:AT3G62720.
NMPDR	fig|3702.1.peg.17657.
Organism-specific databases
TAIR	At3g62720.
Enzyme and pathway databases
BRENDA	2.4.2.39. 302.
Gene expression databases
ArrayExpress	Q9LZJ3.
GermOnline	AT3G62720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XT1_ARATH

Accession

Primary (citable) accession number: Q9LZJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)