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Protein

Malate synthase

Gene

MLS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Does not seem to be essential for lipid utilization and gluconeogenesis in seedlings.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.1 Publication

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL)
  2. Malate synthase, Malate synthase (MLS), Malate synthase, Malate synthase, Malate synthase (AXX17_At5g03190)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177Proton acceptorCurated1
Active sitei463Proton donorCurated1

GO - Molecular functioni

  • malate synthase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMetaCyc:AT5G03860-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase1 Publication (EC:2.3.3.91 Publication)
Gene namesi
Name:MLS1 Publication
Ordered Locus Names:At5g03860Imported
ORF Names:F8F6.70Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G03860
TAIRilocus:2150625 AT5G03860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but the frequency of mutant seedlings to establish into plantlets with true leaves is decreased under short day conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004308761 – 562Malate synthaseAdd BLAST562

Proteomic databases

PaxDbiQ9LZC3

Expressioni

Developmental stagei

Expressed from 1 to 5 days after seed imbibition (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9LZC3 baseline and differential
GenevisibleiQ9LZC3 AT

Interactioni

Protein-protein interaction databases

BioGridi16966, 1 interactor
STRINGi3702.AT5G03860.1

Structurei

3D structure databases

ProteinModelPortaliQ9LZC3
SMRiQ9LZC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi560 – 562Microbody targeting signalCurated3

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiKOG1261 Eukaryota
COG2225 LUCA
HOGENOMiHOG000238464
InParanoidiQ9LZC3
KOiK01638
OMAiETAHIRA
OrthoDBiEOG093605DO
PhylomeDBiQ9LZC3

Family and domain databases

CDDicd00727 malate_synt_A, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR006252 Malate_synthA
IPR001465 Malate_synthase
IPR019830 Malate_synthase_CS
PANTHERiPTHR42902 PTHR42902, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
PIRSFiPIRSF001363 Malate_synth, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01344 malate_syn_A, 1 hit
PROSITEiView protein in PROSITE
PS00510 MALATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LZC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELETSVYRP NVAVYDSPDG VEVRGRYDQI FAKILTREAL SFVAELQREF
60 70 80 90 100
RGHVKYAMEC RREARRRYNS GAVPGFDPST KFIRDGDWSC ASVPPAVADR
110 120 130 140 150
RVEITGPVER KMIINALNSG AKVFMADFED ALSPSWENLM RGHVNLKDAV
160 170 180 190 200
DGSITFHDKS RNRVYKLNDQ TAKLFVRPRG WHLPEAHILI DGEPATGCLV
210 220 230 240 250
DFGLYFFHNY AKFRQTQGSG FGPFFYLPKM EHSREAKIWN SVFERAEKMA
260 270 280 290 300
GIERGSIRAT VLIETLPAVF QMNEILYELR DHSVGLNCGR WDYIFSYVKT
310 320 330 340 350
FQAHPDRLLP DRVLVGMGQH FMRSYSDLLI RTCHKRGVHA MGGMAAQIPI
360 370 380 390 400
RDDPKANEMA LDLVRKDKLR EVRAGHDGTW AAHPGLIPIC MEAFTGHMGK
410 420 430 440 450
SPNQIKSVKR EDAAAITEED LLQIPRGVRT LEGLRLNTRV GIQYLAAWLT
460 470 480 490 500
GSGSVPLYNL MEDAATAEIS RVQNWQWIRY GVELDGDGLG VRVSKELFGR
510 520 530 540 550
VVEEEMERIE KEVGKDKFKN GMYKEACKMF TKQCTAPELD DFLTLAVYNH
560
IVAHYPINVS RL
Length:562
Mass (Da):63,887
Last modified:October 1, 2000 - v1
Checksum:iF8FAB0D5F5E08049
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005235 Genomic DNA Translation: BAB08612.1
AL162873 Genomic DNA Translation: CAB85506.1
CP002688 Genomic DNA Translation: AED90663.1
CP002688 Genomic DNA Translation: AED90664.1
AF360126 mRNA Translation: AAK25836.1
AY051027 mRNA Translation: AAK93704.1
PIRiT48413
RefSeqiNP_001190219.1, NM_001203290.1
NP_196006.1, NM_120467.4
UniGeneiAt.4884

Genome annotation databases

EnsemblPlantsiAT5G03860.1; AT5G03860.1; AT5G03860
AT5G03860.2; AT5G03860.2; AT5G03860
GeneIDi831690
GrameneiAT5G03860.1; AT5G03860.1; AT5G03860
AT5G03860.2; AT5G03860.2; AT5G03860
KEGGiath:AT5G03860

Similar proteinsi

Entry informationi

Entry nameiMASY_ARATH
AccessioniPrimary (citable) accession number: Q9LZC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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