Q9LZ86 (P2C66_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 66
Short name=AtPP2C66
EC=3.1.3.16

Alternative name(s):
Protein POLTERGEIST-LIKE 2
Protein phosphatase 2C PLL2
Short name=PP2C PLL2

Gene names

Name:	PLL2
Ordered Locus Names:	At5g02400
ORF Names:	T1E22.160

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed at low level in seedlings, roots, leaves, stems, young inflorescences, flowers and siliques. Ref.3
Domain	The conserved PP2C phosphatase domain (244-663) is interrupted by an insertion of approximately 100 amino acids.
Disruption phenotype	No visible phenotype. Ref.3
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Probable protein phosphatase 2C 66		PRO_0000301260
Regions
	Domain	322 – 637	316	PP2C-like
Sites
	Metal binding	282	1	Manganese 1 By similarity
	Metal binding	282	1	Manganese 2 By similarity
	Metal binding	283	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	593	1	Manganese 2 By similarity
	Metal binding	656	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LZ86 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CA2DE97E69791B0D	FASTA	674	74,838
	10 20 30 40 50 60 MGNGVTTLTG CCTGTLAGEI SRRYDVSLVH DGLGHSFCYI RPDLPGVVLP SPESPLRSDH 70 80 90 100 110 120 IQETTFRSIS GASVSANPST ALSGALSSDS DCPYSSAVSA SAFESSGNFA SLPLQPVPRG 130 140 150 160 170 180 STWQSGPIVN ESGLGSAPFE RRFLSGPIES GLYSGPIEST KKTEKEKPKK IRKKPKSKKN 190 200 210 220 230 240 FLTFKTLFAN LISNNNKPRL KKSVIEPING SDSSDSGRLH HEPVITSSRS NENPKSDLEE 250 260 270 280 290 300 EDEKQSMNSV LDVQWAQGKA GEDRVHVVVS EDNGWVFVGI YDGFSGPDAP DYLLNNLYTA 310 320 330 340 350 360 VQKELNGLLW NDEKLRSLGE NGMTKTGKCS DEEDPESGKE NCPVINNDDA VASGARNQAK 370 380 390 400 410 420 SLKWRCEWEK KSNNKTKSDN RCDQKGSNST TTNHKDVLKA LLQALRKTED AYLELADQMV 430 440 450 460 470 480 KENPELALMG SCVLVTLMKG EDVYVMNVGD SRAVLGRKPN LATGRKRQKE LERIREDSSL 490 500 510 520 530 540 EDKEILMNGA MRNTLVPLQL NMEHSTRIEE EVRRIKKEHP DDDCAVENDR VKGYLKVTRA 550 560 570 580 590 600 FGAGFLKQPK WNDALLEMFR IDYIGTSPYI TCSPSLCHHK LTSRDKFLIL SSDGLYEYFS 610 620 630 640 650 660 NQEAIFEVES FISAFPEGDP AQHLIQEVLL RAANKFGMDF HELLEIPQGD RRRYHDDVSV 670 IVISLEGRIW RSSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis." Song S.-K., Clark S.E. Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE, DISRUPTION PHENOTYPE.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL162874 Genomic DNA. Translation: CAB85545.1. CP002688 Genomic DNA. Translation: AED90468.1.
IPI	IPI00546042.
PIR	T48261.
RefSeq	NP_195860.1. NM_120318.1.
UniGene	At.54686.
3D structure databases
ProteinModelPortal	Q9LZ86.
SMR	Q9LZ86. Positions 396-669.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G02400.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G02400.1; AT5G02400.1; AT5G02400.
GeneID	830937.
KEGG	ath:AT5G02400.
Organism-specific databases
TAIR	At5g02400.
Phylogenomic databases
eggNOG	NOG289757.
HOGENOM	HOG000239375.
InParanoid	Q9LZ86.
OMA	CAVENDR.
PhylomeDB	Q9LZ86.
ProtClustDB	CLSN2685134.
Gene expression databases
Genevestigator	Q9LZ86.
Family and domain databases
Gene3D	3.60.40.10. 3 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C66_ARATH

Accession

Primary (citable) accession number: Q9LZ86

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program