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Protein

Assimilatory sulfite reductase (ferredoxin), chloroplastic

Gene

SIR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth.1 Publication
DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication.By similarity

Catalytic activityi

Hydrogen sulfide + 6 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O = sulfite + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • sirohemeBy similarityNote: Binds 1 siroheme per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi503Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi509Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi549Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi553Iron (siroheme axial ligand)By similarity1
Metal bindingi553Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • DNA binding Source: UniProtKB
  • heme binding Source: InterPro
  • sulfite reductase (ferredoxin) activity Source: UniProtKB
  • sulfite reductase activity Source: TAIR

GO - Biological processi

  • DNA packaging Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of DNA replication Source: UniProtKB
  • response to cold Source: TAIR
  • response to salt stress Source: TAIR
  • sulfide oxidation, using siroheme sulfite reductase Source: UniProtKB
  • sulfur compound metabolic process Source: TAIR

Keywordsi

Molecular functionDNA-binding, Oxidoreductase
Ligand4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT5G04590-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Assimilatory sulfite reductase (ferredoxin), chloroplastic (EC:1.8.7.11 Publication)
Short name:
AtSiR
Alternative name(s):
Sulfite reductase (ferredoxin)
Gene namesi
Name:SIR
Ordered Locus Names:At5g04590
ORF Names:T32M21.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G04590
TAIRilocus:2184457 AT5G04590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 61ChloroplastSequence analysisAdd BLAST61
ChainiPRO_000041684462 – 642Assimilatory sulfite reductase (ferredoxin), chloroplasticAdd BLAST581

Post-translational modificationi

Phosphorylated; this phosphorylation reduces DNA-binding.By similarity

Keywords - PTMi

Thioether bond

Proteomic databases

PaxDbiQ9LZ66
PRIDEiQ9LZ66

PTM databases

SwissPalmiQ9LZ66

Expressioni

Tissue specificityi

Present in leaves and roots.1 Publication

Inductioni

Rapidly induced by sulfur dioxide SO2 in a sulfite oxidase (SO)-dependent manner.1 Publication

Gene expression databases

ExpressionAtlasiQ9LZ66 baseline and differential
GenevisibleiQ9LZ66 AT

Interactioni

Subunit structurei

Monomer. Interacts with ferredoxin (By similarity).By similarity

Protein-protein interaction databases

BioGridi15615, 1 interactor
STRINGi3702.AT5G04590.1

Structurei

3D structure databases

ProteinModelPortaliQ9LZ66
SMRiQ9LZ66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0560 Eukaryota
COG0155 LUCA
HOGENOMiHOG000218417
InParanoidiQ9LZ66
KOiK00392
OMAiINRNVMA
OrthoDBiEOG093603A5
PhylomeDBiQ9LZ66

Family and domain databases

InterProiView protein in InterPro
IPR005117 NiRdtase/SiRdtase_haem-b_fer
IPR036136 Nit/Sulf_reduc_fer_like_dom_sf
IPR006067 NO2/SO3_Rdtase_4Fe4S_dom
IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
IPR011787 SiR_ferredoxin-dep
PfamiView protein in Pfam
PF01077 NIR_SIR, 2 hits
PF03460 NIR_SIR_ferr, 2 hits
PRINTSiPR00397 SIROHAEM
SUPFAMiSSF55124 SSF55124, 2 hits
TIGRFAMsiTIGR02042 sir, 1 hit
PROSITEiView protein in PROSITE
PS00365 NIR_SIR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LZ66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTFRAPAG AATVFTADQK IRLGRLDALR SSHSVFLGRY GRGGVPVPPS
60 70 80 90 100
ASSSSSSPIQ AVSTPAKPET ATKRSKVEII KEKSNFIRYP LNEELLTEAP
110 120 130 140 150
NVNESAVQLI KFHGSYQQYN REERGGRSYS FMLRTKNPSG KVPNQLYLTM
160 170 180 190 200
DDLADEFGIG TLRLTTRQTF QLHGVLKQNL KTVMSSIIKN MGSTLGACGD
210 220 230 240 250
LNRNVLAPAA PYVKKDYLFA QETADNIAAL LSPQSGFYYD MWVDGEQFMT
260 270 280 290 300
AEPPEVVKAR NDNSHGTNFV DSPEPIYGTQ FLPRKFKVAV TVPTDNSVDL
310 320 330 340 350
LTNDIGVVVV SDENGEPQGF NIYVGGGMGR THRMESTFAR LAEPIGYVPK
360 370 380 390 400
EDILYAVKAI VVTQREHGRR DDRKYSRMKY LISSWGIEKF RDVVEQYYGK
410 420 430 440 450
KFEPSRELPE WEFKSYLGWH EQGDGAWFCG LHVDSGRVGG IMKKTLREVI
460 470 480 490 500
EKYKIDVRIT PNQNIVLCDI KTEWKRPITT VLAQAGLLQP EFVDPLNQTA
510 520 530 540 550
MACPAFPLCP LAITEAERGI PSILKRVRAM FEKVGLDYDE SVVIRVTGCP
560 570 580 590 600
NGCARPYMAE LGLVGDGPNS YQVWLGGTPN LTQIARSFMD KVKVHDLEKV
610 620 630 640
CEPLFYHWKL ERQTKESFGE YTTRMGFEKL KELIDTYKGV SQ
Length:642
Mass (Da):71,950
Last modified:October 1, 2000 - v1
Checksum:iA00E4F12C278CFA3
GO

Sequence cautioni

The sequence AAK82552 differs from that shown. Reason: Erroneous termination at position 417. Translated as Leu.Curated
The sequence AAN18162 differs from that shown. Reason: Erroneous termination at position 417. Translated as Leu.Curated
The sequence CAA71239 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203R → K in AAK82552 (PubMed:14593172).Curated1
Sequence conflicti203R → K in AAN18162 (PubMed:14593172).Curated1
Sequence conflicti426A → T in CAA89154 (PubMed:8695637).Curated1
Sequence conflicti554A → R in CAA71239 (PubMed:9661674).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49217 mRNA Translation: CAA89154.1
Y10157 Genomic DNA Translation: CAA71239.1 Sequence problems.
AL162875 Genomic DNA Translation: CAB85565.1
CP002688 Genomic DNA Translation: AED90762.1
AF325027 mRNA Translation: AAG40379.1
AY048290 mRNA Translation: AAK82552.1 Sequence problems.
BT000593 mRNA Translation: AAN18162.1 Sequence problems.
AK229873 mRNA Translation: BAF01702.1
PIRiS71437
T48455
RefSeqiNP_196079.1, NM_120541.4
UniGeneiAt.25240

Genome annotation databases

EnsemblPlantsiAT5G04590.1; AT5G04590.1; AT5G04590
GeneIDi830336
GrameneiAT5G04590.1; AT5G04590.1; AT5G04590
KEGGiath:AT5G04590

Similar proteinsi

Entry informationi

Entry nameiSIR_ARATH
AccessioniPrimary (citable) accession number: Q9LZ66
Secondary accession number(s): O23650
, Q0WMF4, Q42590, Q94AB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health