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Protein

DNA topoisomerase 6 subunit A

Gene

TOP6A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity. Involved in cell-elongation processes.UniRule annotation2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170NucleophileUniRule annotation1
Metal bindingi256MagnesiumUniRule annotation1
Metal bindingi308MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 6 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Short name:
AtTOP6A
Alternative name(s):
Meiotic recombination protein SPO11-3
Short name:
AtSPO11-3
Protein BRASSINOSTEROID INSENSITIVE 5
Protein ROOT HAIRLESS 2
Gene namesi
Name:TOP6AUniRule annotation
Synonyms:BIN5, RHL2, SPO11-3
Ordered Locus Names:At5g02820
ORF Names:F9G14.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G02820.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

  • DNA topoisomerase complex (ATP-hydrolyzing) Source: UniProtKB-HAMAP
  • nuclear chromosome Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants are defective in cell elongation and show a severe dwarf phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003461091 – 427DNA topoisomerase 6 subunit AAdd BLAST427

Proteomic databases

PaxDbiQ9LZ03.
PRIDEiQ9LZ03.

Expressioni

Tissue specificityi

Highly expressed in leaves, stems, flowers and seedlings.1 Publication

Gene expression databases

GenevisibleiQ9LZ03. AT.

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two TOP6A and two TOP6B subunits. Interacts with BIN4 and RHL1.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHL1O812422EBI-1772104,EBI-1772159
TOP6BQ9C5V63EBI-1772104,EBI-1772132

GO - Molecular functioni

  • identical protein binding Source: TAIR

Protein-protein interaction databases

BioGridi16591. 1 interactor.
DIPiDIP-40169N.
IntActiQ9LZ03. 2 interactors.
STRINGi3702.AT5G02820.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LZ03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 80Ser-richAdd BLAST45

Sequence similaritiesi

Belongs to the TOP6A family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000225930.
InParanoidiQ9LZ03.
KOiK10878.
OMAiWEEQIEL.
OrthoDBiEOG09360BEL.
PhylomeDBiQ9LZ03.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LZ03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKKRKRS KDDEAEELPF KSILESDDVI TELLKSYISS SIKAAAGAGG
60 70 80 90 100
ASSSSSKPLT LADLSLSSSC REVADLSLSS VQTEIETVIV QIARSILAGD
110 120 130 140 150
GFSFSVPSRA ASNQLYVPEL DRIVLKDKST LRPFASVSSV RKTTITTRIL
160 170 180 190 200
ALIHQLCLRN IHVTKRDLFY TDVKLFQDQT QSDAVLDDVS CMLGCTRSSL
210 220 230 240 250
NVIAAEKGVV VGRLIFSDNG DMIDCTKMGM GGKAIPPNID RVGDMQSDAM
260 270 280 290 300
FILLVEKDAA YMRLAEDRFY NRFPCIIVTA KGQPDVATRL FLRKMKMELK
310 320 330 340 350
LPVLALVDSD PYGLKILSVY GCGSKNMSYD SANLTTPDIK WLGIRPSDLD
360 370 380 390 400
KYKIPEQCRL PMTEQDIKTG KDMLEEDFVK KNPGWVEELN LMVKTKQKAE
410 420
IQALSSFGFQ YLSEVYLPLK LQQQDWL
Length:427
Mass (Da):47,446
Last modified:October 1, 2000 - v1
Checksum:i0EA41B8B4BA77441
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297842 mRNA. Translation: CAC24689.1.
AF323679 mRNA. Translation: AAL01152.1.
AL162973 Genomic DNA. Translation: CAB86036.1.
CP002688 Genomic DNA. Translation: AED90522.1.
BT028966 mRNA. Translation: ABI54341.1.
PIRiT48303.
RefSeqiNP_195902.1. NM_120360.4.
UniGeneiAt.33359.
At.66674.

Genome annotation databases

EnsemblPlantsiAT5G02820.1; AT5G02820.1; AT5G02820.
GeneIDi831314.
GrameneiAT5G02820.1; AT5G02820.1; AT5G02820.
KEGGiath:AT5G02820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297842 mRNA. Translation: CAC24689.1.
AF323679 mRNA. Translation: AAL01152.1.
AL162973 Genomic DNA. Translation: CAB86036.1.
CP002688 Genomic DNA. Translation: AED90522.1.
BT028966 mRNA. Translation: ABI54341.1.
PIRiT48303.
RefSeqiNP_195902.1. NM_120360.4.
UniGeneiAt.33359.
At.66674.

3D structure databases

ProteinModelPortaliQ9LZ03.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16591. 1 interactor.
DIPiDIP-40169N.
IntActiQ9LZ03. 2 interactors.
STRINGi3702.AT5G02820.1.

Proteomic databases

PaxDbiQ9LZ03.
PRIDEiQ9LZ03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G02820.1; AT5G02820.1; AT5G02820.
GeneIDi831314.
GrameneiAT5G02820.1; AT5G02820.1; AT5G02820.
KEGGiath:AT5G02820.

Organism-specific databases

TAIRiAT5G02820.

Phylogenomic databases

eggNOGiKOG2795. Eukaryota.
COG1697. LUCA.
HOGENOMiHOG000225930.
InParanoidiQ9LZ03.
KOiK10878.
OMAiWEEQIEL.
OrthoDBiEOG09360BEL.
PhylomeDBiQ9LZ03.

Miscellaneous databases

PROiQ9LZ03.

Gene expression databases

GenevisibleiQ9LZ03. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOP6A_ARATH
AccessioniPrimary (citable) accession number: Q9LZ03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.