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Protein

DNA topoisomerase 6 subunit A

Gene

TOP6A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity. Involved in cell-elongation processes.UniRule annotation2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170NucleophileUniRule annotation1
Metal bindingi256MagnesiumUniRule annotation1
Metal bindingi308MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 6 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Short name:
AtTOP6A
Alternative name(s):
Meiotic recombination protein SPO11-3
Short name:
AtSPO11-3
Protein BRASSINOSTEROID INSENSITIVE 5
Protein ROOT HAIRLESS 2
Gene namesi
Name:TOP6AUniRule annotation
Synonyms:BIN5, RHL2, SPO11-3
Ordered Locus Names:At5g02820
ORF Names:F9G14.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G02820
TAIRilocus:2151221 AT5G02820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants are defective in cell elongation and show a severe dwarf phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003461091 – 427DNA topoisomerase 6 subunit AAdd BLAST427

Proteomic databases

PaxDbiQ9LZ03
PRIDEiQ9LZ03

Expressioni

Tissue specificityi

Highly expressed in leaves, stems, flowers and seedlings.1 Publication

Gene expression databases

ExpressionAtlasiQ9LZ03 baseline and differential
GenevisibleiQ9LZ03 AT

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two TOP6A and two TOP6B subunits. Interacts with BIN4 and RHL1.UniRule annotation4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi16591, 1 interactor
DIPiDIP-40169N
IntActiQ9LZ03, 3 interactors
STRINGi3702.AT5G02820.1

Structurei

3D structure databases

ProteinModelPortaliQ9LZ03
SMRiQ9LZ03
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 80Ser-richAdd BLAST45

Sequence similaritiesi

Belongs to the TOP6A family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2795 Eukaryota
COG1697 LUCA
HOGENOMiHOG000225930
InParanoidiQ9LZ03
KOiK10878
OMAiFTDGDPY
OrthoDBiEOG09360BEL
PhylomeDBiQ9LZ03

Family and domain databases

CDDicd00223 TOPRIM_TopoIIB_SPO, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00132 Top6A, 1 hit
InterProiView protein in InterPro
IPR002815 Spo11/TopoVI_A
IPR013049 Spo11/TopoVI_A_N
IPR036078 Spo11/TopoVI_A_sf
IPR004085 TopoVI_A
IPR034136 TOPRIM_Topo6A/Spo11
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10848 PTHR10848, 1 hit
PfamiView protein in Pfam
PF04406 TP6A_N, 1 hit
PRINTSiPR01550 TOP6AFAMILY
PR01552 TPISMRASE6A
SUPFAMiSSF56726 SSF56726, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LZ03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKKRKRS KDDEAEELPF KSILESDDVI TELLKSYISS SIKAAAGAGG
60 70 80 90 100
ASSSSSKPLT LADLSLSSSC REVADLSLSS VQTEIETVIV QIARSILAGD
110 120 130 140 150
GFSFSVPSRA ASNQLYVPEL DRIVLKDKST LRPFASVSSV RKTTITTRIL
160 170 180 190 200
ALIHQLCLRN IHVTKRDLFY TDVKLFQDQT QSDAVLDDVS CMLGCTRSSL
210 220 230 240 250
NVIAAEKGVV VGRLIFSDNG DMIDCTKMGM GGKAIPPNID RVGDMQSDAM
260 270 280 290 300
FILLVEKDAA YMRLAEDRFY NRFPCIIVTA KGQPDVATRL FLRKMKMELK
310 320 330 340 350
LPVLALVDSD PYGLKILSVY GCGSKNMSYD SANLTTPDIK WLGIRPSDLD
360 370 380 390 400
KYKIPEQCRL PMTEQDIKTG KDMLEEDFVK KNPGWVEELN LMVKTKQKAE
410 420
IQALSSFGFQ YLSEVYLPLK LQQQDWL
Length:427
Mass (Da):47,446
Last modified:October 1, 2000 - v1
Checksum:i0EA41B8B4BA77441
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297842 mRNA Translation: CAC24689.1
AF323679 mRNA Translation: AAL01152.1
AL162973 Genomic DNA Translation: CAB86036.1
CP002688 Genomic DNA Translation: AED90522.1
BT028966 mRNA Translation: ABI54341.1
PIRiT48303
RefSeqiNP_195902.1, NM_120360.4
UniGeneiAt.33359
At.66674

Genome annotation databases

EnsemblPlantsiAT5G02820.1; AT5G02820.1; AT5G02820
GeneIDi831314
GrameneiAT5G02820.1; AT5G02820.1; AT5G02820
KEGGiath:AT5G02820

Similar proteinsi

Entry informationi

Entry nameiTOP6A_ARATH
AccessioniPrimary (citable) accession number: Q9LZ03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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