SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9LYT7

- LEUD2_ARATH

UniProt

Q9LYT7 - LEUD2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
3-isopropylmalate dehydratase small subunit 2
Gene
IPMI1, At3g58990, F17J16.40
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. Functions redundantly with LEUD1 in aliphatic glucosinolate biosynthesis.1 Publication

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Pathwayi

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB

GO - Biological processi

  1. glucosinolate biosynthetic process Source: UniProtKB
  2. leucine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G58990-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunit 2 (EC:4.2.1.33)
Alternative name(s):
AtLEUD2
Isopropylmalate isomerase small subunit 1
Gene namesi
Name:IPMI1
Ordered Locus Names:At3g58990
ORF Names:F17J16.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G58990.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB
  2. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5656Chloroplast Reviewed prediction
Add
BLAST
Chaini57 – 2531973-isopropylmalate dehydratase small subunit 2
PRO_0000425811Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriQ9LYT7.

Interactioni

Subunit structurei

Heterodimer of LEUC/IIL1 and LEUD (IPMI1, IPMI2 or At2g43090).1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G58990.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LYT7.
SMRiQ9LYT7. Positions 70-246.

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0066.
HOGENOMiHOG000222940.
InParanoidiQ9LYT7.
KOiK01704.
OMAiYCMEDAR.
PhylomeDBiQ9LYT7.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
InterProiIPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LYT7-1 [UniParc]FASTAAdd to Basket

« Hide

MATSQQFLNP TLFKSLASSN KNSCTLCPSP FLQLKSASTI FNYKPLTSSS    50
ATIITRVAAS SSDSGESITR ETFHGLCFVL KDNIDTDQII PAEYGTLIPS 100
IPEDREKLGS FALNGLPKFY NERFVVPGEM KSKYSVIIGG DNFGCGSSRE 150
HAPVCLGAAG AKAVVAESYA RIFFRNCVAT GEIFPLESEV RICDECKTGD 200
VVTIEHKEDG SSLLINHTTR KEYKLKPLGD AGPVIDAGGI FAYARKAGMI 250
PSA 253
Length:253
Mass (Da):27,208
Last modified:October 1, 2000 - v1
Checksum:iA99AB88C4656B0DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL163527 Genomic DNA. Translation: CAB86927.1.
CP002686 Genomic DNA. Translation: AEE79857.1.
AY065366 mRNA. Translation: AAL38807.1.
BT006060 mRNA. Translation: AAP04045.1.
PIRiT47781.
RefSeqiNP_191458.1. NM_115761.3.
UniGeneiAt.26842.

Genome annotation databases

EnsemblPlantsiAT3G58990.1; AT3G58990.1; AT3G58990.
GeneIDi825068.
KEGGiath:AT3G58990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL163527 Genomic DNA. Translation: CAB86927.1 .
CP002686 Genomic DNA. Translation: AEE79857.1 .
AY065366 mRNA. Translation: AAL38807.1 .
BT006060 mRNA. Translation: AAP04045.1 .
PIRi T47781.
RefSeqi NP_191458.1. NM_115761.3.
UniGenei At.26842.

3D structure databases

ProteinModelPortali Q9LYT7.
SMRi Q9LYT7. Positions 70-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G58990.1-P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G58990.1 ; AT3G58990.1 ; AT3G58990 .
GeneIDi 825068.
KEGGi ath:AT3G58990.

Organism-specific databases

TAIRi AT3G58990.

Phylogenomic databases

eggNOGi COG0066.
HOGENOMi HOG000222940.
InParanoidi Q9LYT7.
KOi K01704.
OMAi YCMEDAR.
PhylomeDBi Q9LYT7.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci ARA:AT3G58990-MONOMER.

Gene expression databases

Genevestigatori Q9LYT7.

Family and domain databases

Gene3Di 3.20.19.10. 1 hit.
InterProi IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00694. Aconitase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52016. SSF52016. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis."
    He Y., Chen B., Pang Q., Strul J.M., Chen S.
    Plant Cell Physiol. 51:1480-1487(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLEUD2_ARATH
AccessioniPrimary (citable) accession number: Q9LYT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi