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Protein

3-isopropylmalate dehydratase small subunit 2

Gene

IPMI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. Functions redundantly with LEUD1 in aliphatic glucosinolate biosynthesis.1 Publication

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase 2, chloroplastic (IPMS2), 2-isopropylmalate synthase 1, chloroplastic (IPMS1)
  2. 3-isopropylmalate dehydratase small subunit 1 (IPMI2), 3-isopropylmalate dehydratase small subunit 3 (At2g43090), 3-isopropylmalate dehydratase small subunit 2 (IPMI1), 3-isopropylmalate dehydratase large subunit, chloroplastic (IIL1)
  3. 3-isopropylmalate dehydrogenase (AXX17_At5g13660), 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase (IMD3), 3-isopropylmalate dehydrogenase (At1g31180/F28K20_14), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (IMD1), 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3), 3-isopropylmalate dehydrogenase 2, chloroplastic (IMDH2), 3-isopropylmalate dehydrogenase 1, chloroplastic (IMDH1), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase (AXX17_At1g31810), 3-isopropylmalate dehydrogenase
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • 3-isopropylmalate dehydratase activity Source: UniProtKB

GO - Biological processi

  • glucosinolate biosynthetic process Source: UniProtKB
  • leucine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G58990-MONOMER
BRENDAi4.2.1.33 399
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunit 2 (EC:4.2.1.33)
Alternative name(s):
AtLEUD2
Isopropylmalate isomerase small subunit 1
Gene namesi
Name:IPMI1
Ordered Locus Names:At3g58990
ORF Names:F17J16.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G58990
TAIRilocus:2077685 AT3G58990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56ChloroplastSequence analysisAdd BLAST56
ChainiPRO_000042581157 – 2533-isopropylmalate dehydratase small subunit 2Add BLAST197

Proteomic databases

PaxDbiQ9LYT7
PRIDEiQ9LYT7

Expressioni

Gene expression databases

ExpressionAtlasiQ9LYT7 baseline and differential
GenevisibleiQ9LYT7 AT

Interactioni

Subunit structurei

Heterodimer of LEUC/IIL1 and LEUD (IPMI1, IPMI2 or At2g43090).1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G58990.1

Structurei

3D structure databases

ProteinModelPortaliQ9LYT7
SMRiQ9LYT7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IQBY Eukaryota
COG0066 LUCA
HOGENOMiHOG000222940
InParanoidiQ9LYT7
KOiK21359
OMAiITPGRYN
OrthoDBiEOG09360R9R
PhylomeDBiQ9LYT7

Family and domain databases

Gene3Di3.20.19.10, 1 hit
InterProiView protein in InterPro
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PfamiView protein in Pfam
PF00694 Aconitase_C, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LYT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSQQFLNP TLFKSLASSN KNSCTLCPSP FLQLKSASTI FNYKPLTSSS
60 70 80 90 100
ATIITRVAAS SSDSGESITR ETFHGLCFVL KDNIDTDQII PAEYGTLIPS
110 120 130 140 150
IPEDREKLGS FALNGLPKFY NERFVVPGEM KSKYSVIIGG DNFGCGSSRE
160 170 180 190 200
HAPVCLGAAG AKAVVAESYA RIFFRNCVAT GEIFPLESEV RICDECKTGD
210 220 230 240 250
VVTIEHKEDG SSLLINHTTR KEYKLKPLGD AGPVIDAGGI FAYARKAGMI

PSA
Length:253
Mass (Da):27,208
Last modified:October 1, 2000 - v1
Checksum:iA99AB88C4656B0DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163527 Genomic DNA Translation: CAB86927.1
CP002686 Genomic DNA Translation: AEE79857.1
AY065366 mRNA Translation: AAL38807.1
BT006060 mRNA Translation: AAP04045.1
PIRiT47781
RefSeqiNP_191458.1, NM_115761.3
UniGeneiAt.26842

Genome annotation databases

EnsemblPlantsiAT3G58990.1; AT3G58990.1; AT3G58990
GeneIDi825068
GrameneiAT3G58990.1; AT3G58990.1; AT3G58990
KEGGiath:AT3G58990

Similar proteinsi

Entry informationi

Entry nameiLEUD2_ARATH
AccessioniPrimary (citable) accession number: Q9LYT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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