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Q9LYT7

- LEUD2_ARATH

UniProt

Q9LYT7 - LEUD2_ARATH

Protein

3-isopropylmalate dehydratase small subunit 2

Gene

IPMI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. Functions redundantly with LEUD1 in aliphatic glucosinolate biosynthesis.1 Publication

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

    Pathwayi

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB

    GO - Biological processi

    1. glucosinolate biosynthetic process Source: UniProtKB
    2. leucine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT3G58990-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase small subunit 2 (EC:4.2.1.33)
    Alternative name(s):
    AtLEUD2
    Isopropylmalate isomerase small subunit 1
    Gene namesi
    Name:IPMI1
    Ordered Locus Names:At3g58990
    ORF Names:F17J16.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G58990.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB
    2. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5656ChloroplastSequence AnalysisAdd
    BLAST
    Chaini57 – 2531973-isopropylmalate dehydratase small subunit 2PRO_0000425811Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LYT7.

    Interactioni

    Subunit structurei

    Heterodimer of LEUC/IIL1 and LEUD (IPMI1, IPMI2 or At2g43090).1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT3G58990.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LYT7.
    SMRiQ9LYT7. Positions 70-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LeuD family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0066.
    HOGENOMiHOG000222940.
    InParanoidiQ9LYT7.
    KOiK01704.
    OMAiYCMEDAR.
    PhylomeDBiQ9LYT7.

    Family and domain databases

    Gene3Di3.20.19.10. 1 hit.
    InterProiIPR015937. Acoase/IPM_deHydtase.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00694. Aconitase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52016. SSF52016. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LYT7-1 [UniParc]FASTAAdd to Basket

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    MATSQQFLNP TLFKSLASSN KNSCTLCPSP FLQLKSASTI FNYKPLTSSS    50
    ATIITRVAAS SSDSGESITR ETFHGLCFVL KDNIDTDQII PAEYGTLIPS 100
    IPEDREKLGS FALNGLPKFY NERFVVPGEM KSKYSVIIGG DNFGCGSSRE 150
    HAPVCLGAAG AKAVVAESYA RIFFRNCVAT GEIFPLESEV RICDECKTGD 200
    VVTIEHKEDG SSLLINHTTR KEYKLKPLGD AGPVIDAGGI FAYARKAGMI 250
    PSA 253
    Length:253
    Mass (Da):27,208
    Last modified:October 1, 2000 - v1
    Checksum:iA99AB88C4656B0DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163527 Genomic DNA. Translation: CAB86927.1.
    CP002686 Genomic DNA. Translation: AEE79857.1.
    AY065366 mRNA. Translation: AAL38807.1.
    BT006060 mRNA. Translation: AAP04045.1.
    PIRiT47781.
    RefSeqiNP_191458.1. NM_115761.3.
    UniGeneiAt.26842.

    Genome annotation databases

    EnsemblPlantsiAT3G58990.1; AT3G58990.1; AT3G58990.
    GeneIDi825068.
    KEGGiath:AT3G58990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163527 Genomic DNA. Translation: CAB86927.1 .
    CP002686 Genomic DNA. Translation: AEE79857.1 .
    AY065366 mRNA. Translation: AAL38807.1 .
    BT006060 mRNA. Translation: AAP04045.1 .
    PIRi T47781.
    RefSeqi NP_191458.1. NM_115761.3.
    UniGenei At.26842.

    3D structure databases

    ProteinModelPortali Q9LYT7.
    SMRi Q9LYT7. Positions 70-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G58990.1-P.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G58990.1 ; AT3G58990.1 ; AT3G58990 .
    GeneIDi 825068.
    KEGGi ath:AT3G58990.

    Organism-specific databases

    TAIRi AT3G58990.

    Phylogenomic databases

    eggNOGi COG0066.
    HOGENOMi HOG000222940.
    InParanoidi Q9LYT7.
    KOi K01704.
    OMAi YCMEDAR.
    PhylomeDBi Q9LYT7.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci ARA:AT3G58990-MONOMER.

    Gene expression databases

    Genevestigatori Q9LYT7.

    Family and domain databases

    Gene3Di 3.20.19.10. 1 hit.
    InterProi IPR015937. Acoase/IPM_deHydtase.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00694. Aconitase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52016. SSF52016. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis."
      He Y., Chen B., Pang Q., Strul J.M., Chen S.
      Plant Cell Physiol. 51:1480-1487(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLEUD2_ARATH
    AccessioniPrimary (citable) accession number: Q9LYT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 19, 2014
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3